EV MemProt Atlas

Basic Information11

Protein Names

Disabled homolog 2 (Adaptor molecule disabled-2) (Differentially expressed in ovarian carcinoma 2) (DOC-2) (Differentially-expressed protein 2)

Protein Class (2)

Plasma proteinsPredicted intracellular proteins

Protein Function

Predicted intracellular proteins

Ensembl

ENSG00000153071

Entrez Gene Symbol

DAB2

Gene Synonym

DOC-2

Gene Description

DAB adaptor protein 2

Chromosome

5

Position

39371675-39462300

Supporting publications (n)

14

EVMP confidence score

0.60

Fluorescence & Localization2

Cell SpecificMyonuclei

Function & Pathway8

Protein Function

Predicted intracellular proteins

Cellular Component (11)

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Molecular Function (5)

Biological Process (3)

KEGG

hsa04144 Endocytosis

Reactome (6)

Canonical Pathways (3)

M266 Pid ncadherin pathway
M142 Pid ajdiss 2pathway
M50 Pid ptp1b pathway

Mediation Categories (3)

Adhesion and uptake mediationFusion and delivery mediationReceptor-signaling mediation

Relations & Evidence41

Enzyme-Mediated Modification (13)

13 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
DAB2	PRKCD	Q05655	S	24	phosphorylation	BEL-Large-Corpus_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperKEASIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	KEA:11812785 SIGNOR:10542228 ProtMapper:15280374 KEA:10542228 ProtMapper:10542228 SIGNOR:15280374
DAB2	PRKCG	P05129	S	24	phosphorylation	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperKEASIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	KEA:11812785 ProtMapper:10542228 KEA:10542228 SIGNOR:10542228
DAB2	PRKCB	P05771	S	24	phosphorylation	BEL-Large-Corpus_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperKEASIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	KEA:11812785 ProtMapper:10542228 KEA:10542228 SIGNOR:10542228
DAB2	ROCK2	O75116	S	723	phosphorylation	PhosphoSitePhosphoSite_ProtMapperProtMapper
DAB2	CAMK2G	Q13555	S	723	phosphorylation	PhosphoSitePhosphoSite_ProtMapperProtMapper
DAB2	PRKCA	P17252	S	24	phosphorylation	BEL-Large-Corpus_ProtMapperPhosphoNetworksphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPProtMapperKEA	KEA:17570479 KEA:11812785 KEA:10542228 ProtMapper:15212693
DAB2	PRKCE	Q02156	S	24	phosphorylation	MIMPHPRD_MIMPphosphoELM_MIMPPhosphoSite_MIMP
DAB2	CDK2	P24941	S	227	phosphorylation	KEA	KEA:17570479
DAB2	CDK2	P24941	S	401	phosphorylation	KEA	KEA:17570479
DAB2	GSK3B	P49841	S	401	phosphorylation	KEA	KEA:17570479

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Ligand-Receptor Signaling (5)

5 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No

Regulatory Interaction Network (12)

12 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
DAB2	P98082	SMAD2	Q15796	Yes	Yes	No	WangNetPathHPRDCui2007SignaLink3CancerCellMapSPIKE_LCSPIKE	SPIKE:11387212 CancerCellMap:11387212 HPRD:11387212 NetPath:11387212 SPIKE_LC:16713569 SignaLink3:23331499 SPIKE_LC:11387212 SignaLink3:11387212 SPIKE:16713569
DAB2	P98082	DVL3	Q92997	Yes	Yes	Yes	WangNetPathHPRDCui2007SignaLink3ACSNCancerCellMapSPIKE_LCSPIKE	SPIKE:16713569 ACSN:19581931 ACSN:21304492 SPIKE_LC:12805222 SPIKE:12805222 NetPath:12805222 ACSN:17318175 ACSN:15518237 ACSN:21498506 SPIKE_LC:16713569 ACSN:20460648 SignaLink3:23331499 SignaLink3:12805222 CancerCellMap:12805222 SignaLink3:21071413 ACSN:19561074 HPRD:12805222
DAB2	P98082	TGFR2	P37173	Yes	Yes	No	WangNetPathHPRDCui2007SignaLink3CancerCellMapSPIKE_LCSPIKE	SPIKE:11387212 CancerCellMap:11387212 HPRD:11387212 NetPath:11387212 SPIKE_LC:16713569 SignaLink3:23331499 SPIKE_LC:11387212 SignaLink3:11387212 SPIKE:16713569
DAB2	P98082	TGFR1	P36897	Yes	Yes	No	WangNetPathHPRDCui2007SignaLink3SPIKE_LCSPIKE	SPIKE:11387212 HPRD:11387212 NetPath:11387212 SPIKE_LC:16713569 SignaLink3:23331499 SPIKE_LC:11387212 SignaLink3:11387212 SPIKE:16713569
DAB2	P98082	CSK	P41240	Yes	Yes	No	HPRDSPIKE_LCSignaLink3	SignaLink3:23331499 SignaLink3:12473651 SPIKE_LC:16713569 HPRD:12473651
KPCD	Q05655	DAB2	P98082	Yes	Yes	Yes	HPRD_MIMPSIGNORProtMapperPhosphoSite_KEAHPRDCui2007CancerCellMapWangPhosphoSite_ProtMapperBEL-Large-Corpus_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPiPTMnetPhosphoPointKEAHPRD_KEASIGNOR_ProtMapperREACH_ProtMapperPhosphoSite	KEA:11812785 SIGNOR:10542228 PhosphoSite:15280374 CancerCellMap:10542228 PhosphoSite:10542228 ProtMapper:15280374 KEA:10542228 PhosphoSite:11812785 ProtMapper:10542228 ProtMapper:30845955 SIGNOR:15280374 HPRD:10542228
	COMPLEX:O94973_P53680_P63010_Q96CW1	DAB2	P98082	Yes	Yes	No	SIGNOR	SIGNOR:11247302
DAB2	P98082	LRP6	O75581	Yes	No	Yes	SIGNORACSN	ACSN:19581931 ACSN:21304492 ACSN:17318175 ACSN:15518237 ACSN:21498506 ACSN:20460648 SIGNOR:22491013 ACSN:19561074
KPCG	P05129	DAB2	P98082	Yes	Yes	No	WangphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetPhosphoPointSIGNORProtMapperHPRDCui2007PhosphoSite_KEAKEAHPRD_KEACancerCellMapSIGNOR_ProtMapperREACH_ProtMapperPhosphoSite_ProtMapper	KEA:11812785 SIGNOR:10542228 CancerCellMap:10542228 KEA:10542228 ProtMapper:10542228 ProtMapper:30845955 HPRD:10542228
DAB2	P98082	DAB2P	Q5VWQ8	Yes	Yes	No	SIGNOR	SIGNOR:27858941

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Protein Complex Composition (10)

10 records.

Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs
CLTC CZIB DAB2 EPS15 FCHO1 FCHO2 LRRC59 SLC25A41	O14526 P42566 P98082 Q00610 Q0JRZ9 Q8N5S1 Q96AG4 Q9NWV4	0:0:0:0:0:0:0:0	hu.MAP2
DAB2 EPS15 FCHO1 FCHO2 SLC25A41	O14526 P42566 P98082 Q0JRZ9 Q8N5S1	0:0:0:0:0	hu.MAP2
DAB2 FCHO1 FCHO2 SLC25A41	O14526 P98082 Q0JRZ9 Q8N5S1	0:0:0:0	hu.MAP2
ASNSD1 CADPS CLIP1 DAB2IP IDO2 RFPL1 RFPL2 RFPL3 ZNF410	O75677 O75678 O75679 P30622 Q5VWQ8 Q6ZQW0 Q86VK4 Q9NWL6 Q9ULU8	0:0:0:0:0:0:0:0:0	hu.MAP2
DAB2IP RFPL1 RFPL2 RFPL3	O75677 O75678 O75679 Q5VWQ8	0:0:0:0	hu.MAP
ASNSD1 DAB2IP RFPL1 RFPL2 RFPL3	O75677 O75678 O75679 Q5VWQ8 Q9NWL6	0:0:0:0:0	hu.MAPhu.MAP2
CADPS CLIP1 DAB2IP RFPL1 RFPL3	O75677 O75679 P30622 Q5VWQ8 Q9ULU8	0:0:0:0:0	hu.MAP2
DAB2	P98082	2	PDB	PDB:6o5oPDB:6ovf
DAB2 FCHO2	P98082 Q0JRZ9	0:0	hu.MAP2
DAB2 SLC25A41	P98082 Q8N5S1	0:0	hu.MAP

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion Chromatography	Mass spectrometry	1	27605433

Sequence, Structure & Domains14

Sequences

Length

770

Mass

82,448

Sequence

MSNEVETSATNGQPDQQAAPKAPSKKEKKKGPEKTDEYLLARFKGDGVKYKAKLIGIDDVPDARGDKMSQDSMMKLKGMAAAGRSQGQHKQRIWVNISLSGIKIIDEKTGVIEHEHPVNKISFIARDVTDNRAFGYVCGGEGQHQFFAIKTGQQAEPLVVDLKDLFQVIYNVKKKEEEKKKIEEASKAVENGSEALMILDDQTNKLKSGVDQMDLFGDMSTPPDLNSPTESKDILLVDLNSEIDTNQNSLRENPFLTNGITSCSLPRPTPQASFLPENAFSANLNFFPTPNPDPFRDDPFTQPDQSTPSSFDSLKSPDQKKENSSSSSTPLSNGPLNGDVDYFGQQFDQISNRTGKQEAQAGPWPFSSSQTQPAVRTQNGVSEREQNGFSVKSSPNPFVGSPPKGLSIQNGVKQDLESSVQSSPHDSIAIIPPPQSTKPGRGRRTAKSSANDLLASDIFAPPVSEPSGQASPTGQPTALQPNPLDLFKTSAPAPVGPLVGLGGVTVTLPQAGPWNTASLVFNQSPSMAPGAMMGGQPSGFSQPVIFGTSPAVSGWNQPSPFAASTPPPVPVVWGPSASVAPNAWSTTSPLGNPFQSNIFPAPAVSTQPPSMHSSLLVTPPQPPPRAGPPKDISSDAFTALDPLGDKEIKDVKEMFKDFQLRQPPAVPARKGEQTSSGTLSAFASYFNSKVGIPQENADHDDFDANQLLNKINEPPKPAPRQVSLPVTKSTDNAFENPFFKDSFGSSQASVASSQPVSSEMYRDPFGNPFA

Alternative Products

Event=Alternative splicing; Named isoforms=3; Name=1; IsoId=P98082-1; Sequence=Displayed; Name=2; IsoId=P98082-2; Sequence=VSP_004181; Name=3; IsoId=P98082-3; Sequence=VSP_038401

Alternative Sequence

209..229; Missing (in isoform 3); 230..447; Missing (in isoform 2)

3D Structural Models

Turn

44..46; 107..109

Helix

36..43; 66..85; 118..120; 156..180

Beta Strand

29..31; 48..59; 61..63; 91..98; 101..106; 112..116; 121..126; 133..138; 145..153

3D Structure

NMR spectroscopy (1); X-ray crystallography (2)

Domain & Motif Annotations

Compositional Bias

1..16; Polar residues; 302..313; Polar residues; 366..396; Polar residues; 407..425; Polar residues; 466..480; Polar residues; 604..616; Polar residues; 745..758; Low complexity

Motif

293..295; DPF 1; 298..300; DPF 2

Domain (CC)

The PID domain binds to predominantly non-phosphorylated NPXY internalization motifs present in members of the LDLR and APP family; it also mediates simultaneous binding to phosphatidylinositol 4,5-bisphosphate.; DOMAIN: The Asn-Pro-Phe (NPF) motifs, which are found in proteins involved in the endocytic pathway, mediate the interaction with the EH domain of EPS15, EPS15R and ITSN1..

Domain (FT)

45..196; PID

Region

1..38; Disordered; 230..447; Required for localization to clathrin-coated pits; 284..482; Disordered; 604..732; Sufficient for interaction with GRB2; 604..629; Disordered; 619..627; Required for interaction with CSK; 649..770; Required for interaction with MYO6; 663..671; Required for interaction with GRB2 and CSK; 709..725; Sufficient for interaction with SH3KBP1 SH3 domain; 742..770; Disordered

Clinical Relevance5

Disease Involvement

Tumor suppressor

Antibody (2)

CAB009314 HPA028888

Interaction Protein (5)

ENSG00000147010ENSG00000166949ENSG00000175387ENSG00000177885ENSG00000196586

Interaction Count

5

Interaction Dataset

intact_biogrid

Supporting Publications14

PMID	Title	Abstract
26538482	Insights into immune responses in oral cancer through proteomic analysis of saliva and salivary extracellular vesicles.	No abstract available
30071318	Changes in the urinary extracellular vesicle proteome are associated with nephronophthisis-related ciliopathies.	No abstract available
31508500	Proteomic profiling of extracellular vesicles allows for human breast cancer subtyping.	No abstract available
32795414	Extracellular Vesicle and Particle Biomarkers Define Multiple Human Cancers.	Among traditional exosome markers, CD9, HSPA8, ALIX, and HSP90AB1 represent pan-EVP markers, while ACTB, MSN, and RAP1B are novel pan-EVP markers.
33114768	Proteomic Profiling of Two Distinct Populations of Extracellular Vesicles Isolated from Human Seminal Plasma.	No abstract available
33718342	Extracellular Vesicles Derived From Adult and Fetal Bone Marrow Mesenchymal Stromal Cells Differentially Promote ex vivo Expansion of Hematopoietic Stem and Progenitor Cells.	No abstract available
37033249	Altered ureido protein modification profiles in seminal plasma extracellular vesicles of non-normozoospermic men.	No abstract available
37686366	Identification of a Non-Invasive Urinary Exosomal Biomarker for Diabetic Nephropathy Using Data-Independent Acquisition Proteomics.	No abstract available
37702715	One-Pot Analytical Pipeline for Efficient and Sensitive Proteomic Analysis of Extracellular Vesicles.	No abstract available
37862381	Surfaceome analysis of extracellular vesicles from senescent cells uncovers uptake repressor DPP4.	No abstract available

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Entry name DAB2	UniProt ID P98082	EVMP confidence score 0.60
Supporting publications (n) 14	Transmembrane count	Protein classification Plasma proteinsPredicted intracellular proteins