Protein detail

AKA12

A-kinase anchor protein 12 (AKAP-12) (A-kinase anchor protein 250 kDa) (AKAP 250) (Gravin) (Myasthenia gravis autoantigen)

Protein symbol AKA12	UniProt ID Q02952	EVMP score 0.50
Frequency 1	Transmembrane count	Protein classification Cancer-related genesPlasma proteinsPredicted intracellular proteins

EVMP score: annotation confidence score.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information

Protein Names

A-kinase anchor protein 12 (AKAP-12) (A-kinase anchor protein 250 kDa) (AKAP 250) (Gravin) (Myasthenia gravis autoantigen)

Protein Class

Cancer-related genesPlasma proteinsPredicted intracellular proteins

Protein Function

Cancer-related genes:Candidate cancer biomarkers
Predicted intracellular proteins

Ensembl

ENSG00000131016

Entrez Gene Symbol

AKAP12

Gene Synonym

AKAP250SSeCKS

Gene Description

A-kinase anchoring protein 12

Chromosome

Position

151239967-151358559

Frequency

EVMP Score

0.50

Fluorescence & Localization

Tissue SpecifictestisCell SpecificEarly spermatids

Function & Pathway

Protein Function

Cancer-related genes:Candidate cancer biomarkers
Predicted intracellular proteins

Cellular Component

Molecular Function

Biological Process

Reactome

Mediation Categories

Adhesion and uptake mediationFusion and delivery mediationImmune mediationReceptor-signaling mediation

Relations & Evidence

Enzyme-Mediated Modification

9 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
AKAP12	PRKACA	P17612	S	698	phosphorylation	SIGNORPhosphoSitePhosphoSite_ProtMapperProtMapper	SIGNOR:14657015
AKAP12	PRKACA	P17612	S	627	phosphorylation	SIGNORPhosphoSitePhosphoSite_ProtMapperProtMapper	SIGNOR:14657015
AKAP12	PRKACA	P17612	S	772	phosphorylation	SIGNORPhosphoSitePhosphoSite_ProtMapperProtMapper	SIGNOR:14657015
AKAP12	PRKACA	P17612	S	696	phosphorylation	SIGNORPhosphoSitePhosphoSite_ProtMapperProtMapper	SIGNOR:14657015
AKAP12	CDK1	P06493	T	767	phosphorylation	Sparser_ProtMapperSIGNORProtMapperREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper	ProtMapper:24239028 ProtMapper:23787043 ProtMapper:23063527 SIGNOR:23063527
AKAP12	CDK1	P06493	T	618	phosphorylation	PhosphoSite
AKAP12	CCNB1	P14635	T	767	phosphorylation	REACH_ProtMapperSIGNORSparser_ProtMapperProtMapper	ProtMapper:23063527 SIGNOR:23063527
AKAP12	ATR	Q13535	S	732	phosphorylation	Sparser_ProtMapperProtMapperRLIMS-P_ProtMapperREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper	ProtMapper:27683220
AKAP12	EGF	P01133	S	910	phosphorylation	BEL-Large-Corpus_ProtMapperProtMapper	ProtMapper:17081983

Ligand-Receptor Signaling

5 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No

Regulatory Interaction Network

6 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
KAPCA	P17612	AKA12	Q02952	Yes	Yes	No	PhosphoSite_norefSIGNORiPTMnetProtMapperPhosphoSitePhosphoSite_ProtMapper	PhosphoSite:14657015 SIGNOR:14657015
AKA12	Q02952	KAPCA	P17612	Yes	Yes	No	SIGNOR	SIGNOR:14657015
	COMPLEX:P06493_P14635	AKA12	Q02952	Yes	Yes	No	SIGNOR	SIGNOR:23063527
AKA12	Q02952	KPCA	P17252	Yes	Yes	No	HPRDSIGNOR	SIGNOR:14657015 HPRD:11316952
ATR	Q13535	AKA12	Q02952	Yes	Yes	No	Sparser_ProtMapperSIGNORProtMapperRLIMS-P_ProtMapperSPIKE_LCREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper	ProtMapper:27683220 SIGNOR:27683220 PhosphoSite:27683220 SPIKE_LC:18077418
CDK1	P06493	AKA12	Q02952	Yes	Yes	No	Sparser_ProtMapperPhosphoSite_norefSIGNORiPTMnetProtMapperREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper	ProtMapper:24239028 PhosphoSite:23063527 ProtMapper:30414309 ProtMapper:23063527 ProtMapper:23787043 SIGNOR:23063527

Protein Complex Composition

3 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
HT_DM_Cluster210	AKAP12 DYNLT1 DYNLT4	P63172 Q02952 Q5JR98	1:1:1	Compleat	Compleat:HC2423	22036573
	AKAP12 PRPF40B RBM25 SLC30A4	O14863 P49756 Q02952 Q6NWY9	0:0:0:0	hu.MAP
	AKAP12 KMT5A RAD51 SAE1 SUMO2 SUMO3 TP53 UBA2 UBE2I	P04637 P55854 P61956 P63279 Q02952 Q06609 Q9NQR1 Q9UBE0 Q9UBT2	1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC3764

Isolation & Detection Technology

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion ChromatographyImmunoaffinity Capture	Mass spectrometryWestern blotting	4	38113368 32854315 32560723 38207106

Sequence, Structure & Domains

Sequences

Length

1,782

Mass

191,482

Sequence

MGAGSSTEQRSPEQPPEGSSTPAEPEPSGGGPSAEAAPDTTADPAIAASDPATKLLQKNGQLSTINGVAEQDELSLQEGDLNGQKGALNGQGALNSQEEEEVIVTEVGQRDSEDVSKRDSDKEMATKSAVVHDITDDGQEETPEIIEQIPSSESNLEELTQPTESQANDIGFKKVFKFVGFKFTVKKDKTEKPDTVQLLTVKKDEGEGAAGAGDHKDPSLGAGEAASKESEPKQSTEKPEETLKREQSHAEISPPAESGQAVEECKEEGEEKQEKEPSKSAESPTSPVTSETGSTFKKFFTQGWAGWRKKTSFRKPKEDEVEASEKKKEQEPEKVDTEEDGKAEVASEKLTASEQAHPQEPAESAHEPRLSAEYEKVELPSEEQVSGSQGPSEEKPAPLATEVFDEKIEVHQEEVVAEVHVSTVEERTEEQKTEVEETAGSVPAEELVEMDAEPQEAEPAKELVKLKETCVSGEDPTQGADLSPDEKVLSKPPEGVVSEVEMLSSQERMKVQGSPLKKLFTSTGLKKLSGKKQKGKRGGGDEESGEHTQVPADSPDSQEEQKGESSASSPEEPEEITCLEKGLAEVQQDGEAEEGATSDGEKKREGVTPWASFKKMVTPKKRVRRPSESDKEDELDKVKSATLSSTESTASEMQEEMKGSVEEPKPEEPKRKVDTSVSWEALICVGSSKKRARRGSSSDEEGGPKAMGGDHQKADEAGKDKETGTDGILAGSQEHDPGQGSSSPEQAGSPTEGEGVSTWESFKRLVTPRKKSKSKLEEKSEDSIAGSGVEHSTPDTEPGKEESWVSIKKFIPGRRKKRPDGKQEQAPVEDAGPTGANEDDSDVPAVVPLSEYDAVEREKMEAQQAQKSAEQPEQKAATEVSKELSESQVHMMAAAVADGTRAATIIEERSPSWISASVTEPLEQVEAEAALLTEEVLEREVIAEEEPPTVTEPLPENREARGDTVVSEAELTPEAVTAAETAGPLGAEEGTEASAAEETTEMVSAVSQLTDSPDTTEEATPVQEVEGGVPDIEEQERRTQEVLQAVAEKVKEESQLPGTGGPEDVLQPVQRAEAERPEEQAEASGLKKETDVVLKVDAQEAKTEPFTQGKVVGQTTPESFEKAPQVTESIESSELVTTCQAETLAGVKSQEMVMEQAIPPDSVETPTDSETDGSTPVADFDAPGTTQKDEIVEIHEENEVASGTQSGGTEAEAVPAQKERPPAPSSFVFQEETKEQSKMEDTLEHTDKEVSVETVSILSKTEGTQEADQYADEKTKDVPFFEGLEGSIDTGITVSREKVTEVALKGEGTEEAECKKDDALELQSHAKSPPSPVEREMVVQVEREKTEAEPTHVNEEKLEHETAVTVSEEVSKQLLQTVNVPIIDGAKEVSSLEGSPPPCLGQEEAVCTKIQVQSSEASFTLTAAAEEEKVLGETANILETGETLEPAGAHLVLEEKSSEKNEDFAAHPGEDAVPTGPDCQAKSTPVIVSATTKKGLSSDLEGEKTTSLKWKSDEVDEQVACQEVKVSVAIEDLEPENGILELETKSSKLVQNIIQTAVDQFVRTEETATEMLTSELQTQAHVIKADSQDAGQETEKEGEEPQASAQDETPITSAKEESESTAVGQAHSDISKDMSEASEKTMTVEVEGSTVNDQQLEEVVLPSEEEGGGAGTKSVPEDDGHALLAERIEKSLVEPKEDEKGDDVDDPENQNSALADTDASGGLTKESPDTNGPKQKEKEDAQEVELQEGKVHSESDKAITPQAQEELQKQERESAKSELTES

Alternative Products

Event=Alternative splicing; Named isoforms=3; Name=1; Synonyms=Alpha; IsoId=Q02952-1; Sequence=Displayed; Name=2; Synonyms=Beta; IsoId=Q02952-2; Sequence=VSP_004110, VSP_004111; Name=3; Synonyms=Gamma; IsoId=Q02952-3; Sequence=VSP_028133, VSP_028134

Alternative Sequence

1..105; Missing (in isoform 3); 1..98; Missing (in isoform 2); 99..106; EEEVIVTE -> MLGTITIT (in isoform 2); 106; E -> M (in isoform 3)

3D Structural Models

Domain & Motif Annotations

Compositional Bias

16..53; Low complexity; 108..125; Basic and acidic residues; 145..154; Low complexity; 157..168; Polar residues; 226..249; Basic and acidic residues; 315..347; Basic and acidic residues; 363..379; Basic and acidic residues; 423..435; Basic and acidic residues; 446..456; Acidic residues; 458..468; Basic and acidic residues; 528..537; Basic residues; 625..639; Basic and acidic residues; 640..652; Low complexity; 655..674; Basic and acidic residues; 708..724; Basic and acidic residues; 739..749; Polar residues; 792..803; Basic and acidic residues; 986..997; Low complexity; 1072..1089; Basic and acidic residues; 1164..1174; Polar residues; 1187..1198; Basic and acidic residues; 1231..1251; Basic and acidic residues; 1253..1267; Polar residues; 1333..1355; Basic and acidic residues; 1603..1612; Polar residues; 1629..1639; Basic and acidic residues; 1675..1699; Basic and acidic residues; 1734..1757; Basic and acidic residues; 1766..1782; Basic and acidic residues

Motif

607..627; AKAP CaM-binding 1; 756..776; AKAP CaM-binding 2; 801..821; AKAP CaM-binding 3

Domain (CC)

Polybasic regions located between residues 266 and 557 are involved in binding PKC.

Region

1..53; Disordered; 71..169; Disordered; 189..400; Disordered; 266..557; Involved in PKC-binding; 421..886; Disordered; 938..1089; Disordered; 1105..1134; Disordered; 1157..1274; Disordered; 1305..1355; Disordered; 1541..1554; RII-binding; 1584..1782; Disordered

Clinical Relevance

Disease Involvement

Cancer-related genes

Antibody

HPA006344 CAB026379 HPA056230

Interaction Protein

ENSG00000022267ENSG00000114302ENSG00000165868

Interaction Count

Interaction Dataset

intact_biogrid_opencellbiogrid_opencell_bioplex