EV MemProt Atlas

Basic Information11

Protein Names

Tyrosine-protein phosphatase non-receptor type 12 (EC 3.1.3.48) (PTP-PEST) (Protein-tyrosine phosphatase G1) (PTPG1)

Protein Class (2)

EnzymesPredicted intracellular proteins

Protein Function (3)

Enzymes
Predicted intracellular proteins
ENZYME proteins:Hydrolases

Ensembl

ENSG00000127947

Entrez Gene Symbol

PTPN12

Gene Synonym (2)

PTP-PESTPTPG1

Gene Description

Protein tyrosine phosphatase non-receptor type 12

Chromosome

7

Position

77537295-77640069

Supporting publications (n)

12

EVMP confidence score

0.60

Function & Pathway6

Protein Function (3)

Enzymes
Predicted intracellular proteins
ENZYME proteins:Hydrolases

Cellular Component (7)

Molecular Function (5)

Biological Process (3)

Reactome (14)

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Mediation Categories (5)

Adhesion and uptake mediationClinical-translation mediationImmune mediationMetabolism mediationReceptor-signaling mediation

Relations & Evidence49

Enzyme-Mediated Modification (24)

24 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
PTPN12	PRKY	O43930	S	39	phosphorylation	MIMPHPRD_MIMPphosphoELM_MIMPPhosphoSite_MIMP
PTPN12	PRKY	O43930	S	435	phosphorylation	MIMPHPRD_MIMPphosphoELM_MIMPPhosphoSite_MIMP
PTPN12	PRKCB	P05771	S	39	phosphorylation	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPKEA	KEA:17570479
PTPN12	PRKCB	P05771	S	435	phosphorylation	MIMPHPRD_MIMPphosphoELM_MIMPPhosphoSite_MIMP
PTPN12	PRKCG	P05129	S	39	phosphorylation	MIMPHPRD_MIMPphosphoELM_MIMPPhosphoSite_MIMP
PTPN12	PRKCG	P05129	S	435	phosphorylation	MIMPHPRD_MIMPphosphoELM_MIMPPhosphoSite_MIMP
PTPN12	PRKCD	Q05655	S	39	phosphorylation	MIMPHPRD_MIMPphosphoELM_MIMPPhosphoSite_MIMP
PTPN12	PRKCD	Q05655	S	435	phosphorylation	MIMPHPRD_MIMPphosphoELM_MIMPPhosphoSite_MIMP
PTPN12	PRKCE	Q02156	S	39	phosphorylation	MIMPHPRD_MIMPphosphoELM_MIMPPhosphoSite_MIMP
PTPN12	PRKCE	Q02156	S	435	phosphorylation	MIMPHPRD_MIMPphosphoELM_MIMPPhosphoSite_MIMP

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Ligand-Receptor Signaling (5)

5 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No

Regulatory Interaction Network (18)

18 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
PTN12	Q05209	FAK1	Q05397	Yes	No	Yes	SPIKEAdhesomeSIGNORProtMapperDEPODHPRDSIGNOR_ProtMapperREACH_ProtMapperHPRD-phosSPIKE_LC	Adhesome:9497381 HPRD-phos:10400703 SIGNOR:11432829 ProtMapper:19595712 HPRD:11314030 Adhesome:10400703 SPIKE:21876001 HPRD:9497381 HPRD-phos:11314030 SIGNOR:19595712 SPIKE_LC:21876001 HPRD:10400703 Adhesome:11314030 DEPOD:19595712
PTN12	Q05209	WASP	P42768	Yes	No	Yes	SPIKEWangNetPathSIGNORProtMapperDEPODHPRDHINTIntActSIGNOR_ProtMapperHPRD-phosSPIKE_LC	NetPath:14707117 DEPOD:14707117 IntAct:19167335 SIGNOR:11711533 ProtMapper:14707117 SPIKE_LC:19167335 SPIKE:19167335 SPIKE_LC:14707117 HPRD:15588985 HPRD:11711533 IntAct:14707117 HPRD-phos:11711533 HPRD:14707117 SPIKE:14707117 HINT:14707117 SIGNOR:14707117
PTN12	Q05209	JAK2	O60674	Yes	No	Yes	WangNetPathSIGNORProtMapperHPRDCancerCellMapSIGNOR_ProtMapperSPIKESPIKE_LC	SPIKE_LC:11731619 CancerCellMap:11731619 NetPath:11731619 SPIKE:11731619 SIGNOR:11731619 ProtMapper:11731619 HPRD:11731619
PTN12	Q05209	PPIP1	O43586	Yes	No	Yes	SPIKEWangNetPathSIGNORProtMapperDEPODHPRDHINTHuRIIntActSIGNOR_ProtMapperHPRD-phosSPIKE_LC	IntAct:32296183 SPIKE_LC:14707117 HPRD:11711533 HINT:35152348 HPRD-phos:11711533 HINT:9422760 IntAct:35152348 HPRD:9422760 SIGNOR:11711533 HPRD:11971877 SPIKE:14707117 DEPOD:11711533 NetPath:14707117 HINT:14707117 HINT:11711533 HINT:11971877 IntAct:14707117 HPRD-phos:11971877 IntAct:11971877 ProtMapper:11711533
PTN12	Q05209	INSR	P06213	Yes	No	Yes	AdhesomeSIGNORProtMapperHPRDSIGNOR_ProtMapper	Adhesome:19167335 Adhesome:8454633 SIGNOR:8454633 ProtMapper:10734133 ProtMapper:8454633 SIGNOR:10734133 HPRD:8454633
PTN12	Q05209	GIT2	Q14161	Yes	No	Yes	SIGNOR_ProtMapperSIGNORProtMapper	ProtMapper:16317044 SIGNOR:16317044
STK24	Q9Y6E0	PTN12	Q05209	Yes	No	Yes	SIGNOR	SIGNOR:26910843
PTN12	Q05209	BCAR1	P56945	Yes	No	Yes	DOMINOWangAdhesomeSIGNORDEPODHPRDHINTBioGRIDIntActSPIKE_LC	BioGRID:12714323 SIGNOR:11432829 HPRD:12714323 DEPOD:9748319 DOMINO:9285683 SPIKE_LC:9285683 HPRD:9285683 HINT:9748319 HPRD:9920935 Adhesome:9285683 Adhesome:11432829 DEPOD:8887669 Adhesome:8887669 HINT:9285683 IntAct:9285683 HINT:8887669 DEPOD:9920935 DEPOD:9285683 HPRD:8887669 HPRD:9748319 DEPOD:12714323
PTN12	Q05209	GDIR1	P52565	Yes	Yes	No	SIGNOR	SIGNOR:25666508
KPCA	P17252	PTN12	Q05209	Yes	Yes	Yes	HPRD_MIMPSIGNORProtMapperPhosphoSite_KEAphosphoELM_KEAPhosphoNetworksHPRDKinexus_KEAWangPhosphoSite_ProtMapperBEL-Large-Corpus_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPiPTMnetPhosphoPointKEAHPRD_KEAphosphoELMSIGNOR_ProtMapperAdhesomeHPRD-phos	HPRD-phos:18669648 SIGNOR:7520867 KEA:7520867 KEA:94349924 HPRD-phos:18767875 HPRD-phos:7520867 ProtMapper:15212693 ProtMapper:7520867 KEA:7909431 HPRD-phos:20068231 Adhesome:7909431 HPRD:7520867 Adhesome:94349924 ProtMapper:18767875 Adhesome:7520867 ProtMapper:19651622 phosphoELM:7909431 ProtMapper:20068231 ProtMapper:18669648 phosphoELM:7520867 HPRD-phos:19651622

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Protein Complex Composition (1)

1 record.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
	PTPN12	Q05209	4	PDB	PDB:5j8r

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential Ultracentrifugation	Mass spectrometryMass spectrometry [MALDI TOF]	1	40326690

Sequence, Structure & Domains14

Sequences

Length

780

Mass

88,106

Sequence

MEQVEILRKFIQRVQAMKSPDHNGEDNFARDFMRLRRLSTKYRTEKIYPTATGEKEENVKKNRYKDILPFDHSRVKLTLKTPSQDSDYINANFIKGVYGPKAYVATQGPLANTVIDFWRMIWEYNVVIIVMACREFEMGRKKCERYWPLYGEDPITFAPFKISCEDEQARTDYFIRTLLLEFQNESRRLYQFHYVNWPDHDVPSSFDSILDMISLMRKYQEHEDVPICIHCSAGCGRTGAICAIDYTWNLLKAGKIPEEFNVFNLIQEMRTQRHSAVQTKEQYELVHRAIAQLFEKQLQLYEIHGAQKIADGVNEINTENMVSSIEPEKQDSPPPKPPRTRSCLVEGDAKEEILQPPEPHPVPPILTPSPPSAFPTVTTVWQDNDRYHPKPVLHMVSSEQHSADLNRNYSKSTELPGKNESTIEQIDKKLERNLSFEIKKVPLQEGPKSFDGNTLLNRGHAIKIKSASPCIADKISKPQELSSDLNVGDTSQNSCVDCSVTQSNKVSVTPPEESQNSDTPPRPDRLPLDEKGHVTWSFHGPENAIPIPDLSEGNSSDINYQTRKTVSLTPSPTTQVETPDLVDHDNTSPLFRTPLSFTNPLHSDDSDSDERNSDGAVTQNKTNISTASATVSAATSTESISTRKVLPMSIARHNIAGTTHSGAEKDVDVSEDSPPPLPERTPESFVLASEHNTPVRSEWSELQSQERSEQKKSEGLITSENEKCDHPAGGIHYEMCIECPPTFSDKREQISENPTEATDIGFGNRCGKPKGPRDPPSEWT

Alternative Products

Event=Alternative splicing; Named isoforms=3; Name=1; IsoId=Q05209-1; Sequence=Displayed; Name=2; IsoId=Q05209-2; Sequence=VSP_046274; Name=3; IsoId=Q05209-3; Sequence=VSP_054168

Alternative Sequence

1..130; Missing (in isoform 2); 1..119; Missing (in isoform 3)

3D Structural Models

Turn

43..46; 111..113; 270..272

Helix

3..17; 27..42; 51..54; 56..61; 71..73; 114..123; 207..219; 236..252; 262..269; 280..298

Beta Strand

74..76; 90..94; 102..106; 128..131; 135..137; 155..157; 160..170; 173..182; 185..194; 222..225; 227..230; 232..235

3D Structure

NMR spectroscopy (1); X-ray crystallography (2)

Domain & Motif Annotations

Compositional Bias

502..519; Polar residues; 521..533; Basic and acidic residues; 552..577; Polar residues; 587..601; Polar residues; 602..613; Basic and acidic residues; 622..639; Low complexity; 690..703; Polar residues; 704..725; Basic and acidic residues; 771..780; Basic and acidic residues

Domain (FT)

28..293; Tyrosine-protein phosphatase

Region

345..438; Interaction with TGFB1I1; 502..639; Disordered; 657..725; Disordered; 744..780; Disordered

Protein Families (2)

Protein-tyrosine phosphatase family
Non-receptor class 4 subfamily

Sequence Similarities

Belongs to the protein-tyrosine phosphatase family. Non-receptor class 4 subfamily.

Clinical Relevance5

Drugs (3)

SUNITINIB CHEMBL:CHEMBL601167 CRIZOTINIB

Antibody (2)

HPA007038 HPA007097

Interaction Protein (6)

ENSG00000050820ENSG00000089159ENSG00000111679ENSG00000140368ENSG00000146648ENSG00000160691

Interaction Count

6

Interaction Dataset (2)

intact_biogridintact_biogrid_opencell

Supporting Publications12

PMID	Title	Abstract
32854315	Proteomic Profiling of Extracellular Vesicles Derived from Cerebrospinal Fluid of Alzheimer's Disease Patients: A Pilot Study.	Recent studies have highlighted the importance of Aβ and tau-containing extracellular vesicles (EVs) in AD.
33441949	Utility of Claudin-3 in extracellular vesicles from human bile as biomarkers of cholangiocarcinoma.	No abstract available
34815502	The von Willebrand factor stamps plasmatic extracellular vesicles from glioblastoma patients.	No abstract available
36064647	Systemic proteomics and miRNA profile analysis of exosomes derived from human pluripotent stem cells.	No abstract available
36736734	Extracellular vesicle proteomics and phosphoproteomics identify pathways for increased risk in patients hospitalized with COVID-19 and type 2 diabetes mellitus.	No abstract available
37322475	Comprehensive profiling of extracellular vesicles in uveitis and scleritis enables biomarker discovery and mechanism exploration.	No abstract available
38113368	In-Depth Proteome Profiling of Small Extracellular Vesicles Isolated from Cancer Cell Lines and Patient Serum.	No abstract available
38207106	Proteomic, Metabolomic, and Fatty Acid Profiling of Small Extracellular Vesicles from Glioblastoma Stem-Like Cells and Their Role in Tumor Heterogeneity.	No abstract available
40189497	Small extracellular vesicle-based one-step high-throughput microfluidic platform for epithelial ovarian cancer diagnosis.	No abstract available
40465195	Extracellular vesicle proteomics uncovers energy metabolism, complement system, and endoplasmic reticulum stress response dysregulation postexercise in males with myalgic encephalomyelitis/chronic fatigue syndrome.	No abstract available