Protein detail

CD47

Leukocyte surface antigen CD47 (Antigenic surface determinant protein OA3) (Integrin-associated protein) (IAP) (Protein MER6) (CD antigen CD47)

Entry name CD47	UniProt ID Q08722	EVMP confidence score 0.88
Supporting publications (n) 29	Transmembrane count 5	Protein classification CD markersPredicted intracellular proteinsPredicted membrane proteinsTransporters

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information13

Protein Names

Leukocyte surface antigen CD47 (Antigenic surface determinant protein OA3) (Integrin-associated protein) (IAP) (Protein MER6) (CD antigen CD47)

Protein Class (4)

CD markersPredicted intracellular proteinsPredicted membrane proteinsTransporters

Protein Function (3)

Transporters:Transporter channels and pores
CD markers
Predicted intracellular proteins

Transmembrane

142..162; Helical; 177..197; Helical; 208..228; Helical; 236..256; Helical; 269..289; Helical

Transmembrane Count

Ensembl

ENSG00000196776

Entrez Gene Symbol

CD47

Gene Synonym (3)

IAPMER6OA3

Gene Description

CD47 molecule

Chromosome

Position

108043091-108091862

Supporting publications (n)

EVMP confidence score

0.88

Fluorescence & Localization6

Tissue Specificadipose tissueCell SpecificMegakaryocytesSingle-Nuclei Brain Specificendothelial cellBlood Cell SpecificbasophilBlood Lineage Specificgranulocytes

Function & Pathway7

Protein Function (3)

Transporters:Transporter channels and pores
CD markers
Predicted intracellular proteins

Cellular Component (5)

Molecular Function (5)

Biological Process (3)

KEGG (2)

Reactome (8)

Mediation Categories (5)

Adhesion and uptake mediationClinical-translation mediationFusion and delivery mediationImmune mediationReceptor-signaling mediation

Relations & Evidence56

Ligand-Receptor Signaling (54)

54 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
receptor	receptor	CellPhoneDB	No	Yes	No	Yes	No
receptor	receptor	GO_Intercell	No	Yes	No	Yes	No
receptor	receptor	HPMR	No	Yes	No	Yes	No
receptor	receptor	ICELLNET	No	Yes	No	Yes	No
receptor	receptor	CellChatDB	No	Yes	No	Yes	No
receptor	receptor	CellTalkDB	No	Yes	No	Yes	No
receptor	receptor	Ramilowski2015	No	Yes	No	Yes	No
receptor	receptor	Guide2Pharma	No	Yes	No	Yes	No
receptor	receptor	LRdb	No	Yes	No	Yes	No
other_receptor_family	receptor	HPMR	No	Yes	No	Yes	No

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Regulatory Interaction Network (1)

1 record.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
TSP2	P35442	CD47	Q08722	Yes	Yes	No	CellChatDBFantom5_LRdbCellTalkDBHPMR_LRdbiTALKHPMR_CellinkertalklrRamilowski2015SIGNORconnectomeDB2020UniProt_LRdbCellinkerWangLRdbHPMR_talklr	Cellinker:8550562 LRdb:8550562 connectomeDB2020:8550562 CellTalkDB:8550562 SIGNOR:8550562

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential Ultracentrifugation	Mass spectrometry	1	37438638

Sequence, Structure & Domains10

Sequences

Length

323

Mass

35,214

Sequence

MWPLVAALLLGSACCGSAQLLFNKTKSVEFTFCNDTVVIPCFVTNMEAQNTTEVYVKWKFKGRDIYTFDGALNKSTVPTDFSSAKIEVSQLLKGDASLKMDKSDAVSHTGNYTCEVTELTREGETIIELKYRVVSWFSPNENILIVIFPIFAILLFWGQFGIKTLKYRSGGMDEKTIALLVAGLVITVIVIVGAILFVPGEYSLKNATGLGLIVTSTGILILLHYYVFSTAIGLTSFVIAILVIQVIAYILAVVGLSLCIAACIPMHGPLLISGLSILALAQLLGLVYMKFVASNQKTIQPPRKAVEEPLNAFKESKGMMNDE

Alternative Products

Event=Alternative splicing; Named isoforms=4; Name=OA3-323; IsoId=Q08722-1; Sequence=Displayed; Name=OA3-293; IsoId=Q08722-2; Sequence=VSP_002535; Name=OA3-305; IsoId=Q08722-3; Sequence=VSP_002536, VSP_002537; Name=OA3-312; IsoId=Q08722-4; Sequence=VSP_002538

Alternative Sequence

293..323; Missing (in isoform OA3-293); 304..305; KA -> NN (in isoform OA3-305); 306..323; Missing (in isoform OA3-305); 312..323; Missing (in isoform OA3-312)

3D Structural Models

Turn

139..143; 254..260

Helix

51..53; 70..72; 79..81; 88..93; 102..106; 144..164; 178..196; 204..224; 238..253; 268..292

Beta Strand

26..30; 35..39; 42..45; 54..60; 63..69; 74..76; 98..101; 110..118; 121..131; 197..202; 261..264

3D Structure

X-ray crystallography (15)

Domain & Motif Annotations

Domain (FT)

19..127; Ig-like V-type

Clinical Relevance4

Related Diseases (17)

Acute myeloid leukaemia B-cell lymphoma Colorectal cancer Diffuse large B-cell lymphoma Leukaemia Lymphoma Malignant haematopoietic neoplasm Melanoma Multiple myeloma Mycosis fungoides Myelodysplastic syndrome Neuroendocrine carcinoma Non-small-cell lung cancer Ovarian cancer Solid tumour/cancer Squamous cell carcinoma Stomach cancer

Biomarker

Phase 1; Phase 1/2; Phase 2; Phase 3; Phase 2/3

Drugs (5)

EVORPACEPT ABT-510 ONTORPACEPT MAGROLIMAB ANTI-CD47 MONOCLONAL ANTIBODY CC-90002

Antibody (2)

CAB016055 HPA044659

Supporting Publications29

PMID	Title	Abstract
31746532	Responsive Exosome Nano-bioconjugates for Synergistic Cancer Therapy.	Azide-modified exosomes derived from M1 macrophages are conjugated with dibenzocyclooctyne-modified antibodies of CD47 and SIRPα (aCD47 and aSIRPα) through pH-sensitive linkers.
31806995	Checkpoint CD47 Function On Tumor Metastasis And Immune Therapy.	Meanwhile, tumor cells express high CD47 protein which may secrete exosomes with high CD47 expression. The high CD47 expression-exosomes could serve the tumor metastasis process and provide transfer convenience for tumors on the microenvironment.
32944191	A combined "eat me/don't eat me" strategy based on extracellular vesicles for anticancer nanomedicine.	Then, nanocarriers fused to CD47-enriched exosomes originated from human serum were administered to evade phagocytosis by MPS (don't eat me strategy).
33338939	Molecular cargo in myeloid-derived suppressor cells and their exosomes.	Bioassays showed that exosomes induce MDSC chemotaxis dependent on S100A8 and S100A9 in their cargo. Surface selective chemistry identified glycoproteins on MDSC and exosome surfaces, including CD47 and thrombospondin 1, which both facilitate exosome-catalyzed chemotaxis.
34016744	Exosomal CD47 Plays an Essential Role in Immune Evasion in Ovarian Cancer.	CD47 is overexpressed in tumors, facilitates tumor immune evasion, and is located on exosomes. Exosomes were collected from ovarian cancer cell lines, and CD47 expression on exosomes was confirmed via flow cytometry. Inhibition of exosome secretion with GW4869 and exosome uptake with 5-(N-ethyl-N-isopropyl)-amiloride inhibited the surface CD47 expression on ovarian cancer cells and promoted phagocytosis by macrophages.
34108659	Quantitative proteomics identifies the core proteome of exosomes with syntenin-1 as the highest abundant protein and a putative universal biomarker.	Syntenin-1 is also present in exosomes across different species and biofluids, highlighting its potential use as a putative universal biomarker of exosomes. We identified syntenin-1 as a consistently abundant protein in exosomes from different cellular origins.
34147721	Gene-engineered exosomes-thermosensitive liposomes hybrid nanovesicles by the blockade of CD47 signal for combined photothermal therapy and cancer immunotherapy.	No abstract available
34218049	Mononuclear phagocyte system blockade using extracellular vesicles modified with CD47 on membrane surface for myocardial infarction reperfusion injury treatment.	No abstract available
34239692	YAP-Dependent Induction of CD47-Enriched Extracellular Vesicles Inhibits Dendritic Cell Activation and Ameliorates Hepatic Ischemia-Reperfusion Injury.	No abstract available
34841476	CD47 interactions with exportin-1 limit the targeting of m7G-modified RNAs to extracellular vesicles.	CD47 is a marker of self and a signaling receptor for thrombospondin-1 that is also a component of extracellular vesicles (EVs) released by various cell types.

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