Protein detail

M3K12

Mitogen-activated protein kinase kinase kinase 12 (EC 2.7.11.25) (Dual leucine zipper bearing kinase) (DLK) (Leucine-zipper protein kinase) (ZPK) (MAPK-upstream kinase) (MUK) (Mixed lineage kinase)

Entry name M3K12	UniProt ID Q12852	EVMP confidence score 0.50
Supporting publications (n) 2	Transmembrane count	Protein classification EnzymesPredicted intracellular proteinsTransporters

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

Mitogen-activated protein kinase kinase kinase 12 (EC 2.7.11.25) (Dual leucine zipper bearing kinase) (DLK) (Leucine-zipper protein kinase) (ZPK) (MAPK-upstream kinase) (MUK) (Mixed lineage kinase)

Protein Class (3)

EnzymesPredicted intracellular proteinsTransporters

Protein Function (5)

Predicted intracellular proteins
Kinases:STE Ser/Thr protein kinases
ENZYME proteins:Transferases
Enzymes
Transporters:Accessory Factors Involved in Transport

Ensembl

ENSG00000139625

Entrez Gene Symbol

MAP3K12

Gene Synonym (5)

DLKMEKK12MUKZPKZPKP1

Gene Description

Mitogen-activated protein kinase kinase kinase 12

Chromosome

Position

53479669-53500063

Supporting publications (n)

EVMP confidence score

0.50

Fluorescence & Localization2

Tissue Specificskeletal muscleCell SpecificEarly spermatids

Function & Pathway6

Protein Function (5)

Predicted intracellular proteins
Kinases:STE Ser/Thr protein kinases
ENZYME proteins:Transferases
Enzymes
Transporters:Accessory Factors Involved in Transport

Cellular Component (5)

Molecular Function (9)

Biological Process (3)

KEGG

hsa04010 MAPK signaling pathway

Mediation Categories

Receptor-signaling mediation

Relations & Evidence15

Ligand-Receptor Signaling (8)

8 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
receptor	receptor	OmniPath	No	Yes	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
plasma_membrane	plasma_membrane	UniProt_location	No	No	No	No	No
plasma_membrane	plasma_membrane	OmniPath	No	No	No	No	No
receptor	receptor	scConnect	No	Yes	No	No	No

Regulatory Interaction Network (6)

6 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
M3K12	Q12852	MP2K4	P45985	Yes	Yes	No	Sparser_ProtMapperKEGG-MEDICUSSIGNORProtMapperHPRDCui2007SignaLink3WangREACH_ProtMapper	HPRD:10187804 SignaLink3:8637721 ProtMapper:25594179 SignaLink3:23331499 SIGNOR:25594179
JIP1	Q9UQF2	M3K12	Q12852	Yes	Yes	Yes	HPRDSIGNORSignaLink3	HPRD:10490659 SignaLink3:10490659 SignaLink3:23331499 SignaLink3:9868371 SIGNOR:11432832
MBIP1	Q9NS73	M3K12	Q12852	Yes	Yes	No	HPRDLit-BM-17SignaLink3	SignaLink3:10801814 SignaLink3:23331499 Lit-BM-17:10801814 HPRD:10801814
SH3R1	Q7Z6J0	M3K12	Q12852	Yes	Yes	No	HPRDSIGNORSignaLink3	HPRD:14504284 SIGNOR:12514131 SignaLink3:23331499 SIGNOR:11416147 SignaLink3:12514131
M3K12	Q12852	MP2K7	O14733	Yes	Yes	No	KEGG-MEDICUSSIGNORHPRDCui2007SignaLink3Wang	HPRD:10187804 SignaLink3:23331499 SIGNOR:28111074 SignaLink3:15749080
M3K12	Q12852	ML12B	O14950	Yes	Yes	No	PhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetSIGNORProtMapperSIGNOR_ProtMapperPhosphoSite_ProtMapper	SIGNOR:11781833 ProtMapper:11781833

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
	Mass spectrometry	0

Sequence, Structure & Domains15

Sequences

Length

859

Mass

93,219

Sequence

MACLHETRTPSPSFGGFVSTLSEASMRKLDPDTSDCTPEKDLTPTHVLQLHEQDAGGPGGAAGSPESRASRVRADEVRLQCQSGSGFLEGLFGCLRPVWTMIGKAYSTEHKQQQEDLWEVPFEEILDLQWVGSGAQGAVFLGRFHGEEVAVKKVRDLKETDIKHLRKLKHPNIITFKGVCTQAPCYCILMEFCAQGQLYEVLRAGRPVTPSLLVDWSMGIAGGMNYLHLHKIIHRDLKSPNMLITYDDVVKISDFGTSKELSDKSTKMSFAGTVAWMAPEVIRNEPVSEKVDIWSFGVVLWELLTGEIPYKDVDSSAIIWGVGSNSLHLPVPSSCPDGFKILLRQCWNSKPRNRPSFRQILLHLDIASADVLSTPQETYFKSQAEWREEVKLHFEKIKSEGTCLHRLEEELVMRRREELRHALDIREHYERKLERANNLYMELNALMLQLELKERELLRREQALERRCPGLLKPHPSRGLLHGNTMEKLIKKRNVPQKLSPHSKRPDILKTESLLPKLDAALSGVGLPGCPKGPPSPGRSRRGKTRHRKASAKGSCGDLPGLRTAVPPHEPGGPGSPGGLGGGPSAWEACPPALRGLHHDLLLRKMSSSSPDLLSAALGSRGRGATGGAGDPGSPPPARGDTPPSEGSAPGSTSPDSPGGAKGEPPPPVGPGEGVGLLGTGREGTSGRGGSRAGSQHLTPAALLYRAAVTRSQKRGISSEEEEGEVDSEVELTSSQRWPQSLNMRQSLSTFSSENPSDGEEGTASEPSPSGTPEVGSTNTDERPDERSDDMCSQGSEIPLDPPPSEVIPGPEPSSLPIPHQELLRERGPPNSEDSDCDSTELDNSNSVDALRPPASLPP

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q12852-1; Sequence=Displayed; Name=2; IsoId=Q12852-2; Sequence=VSP_044646

Alternative Sequence

46; H -> QCVLRDVVPLGGQGGGGPSPSPGGEPPPEPFANS (in isoform 2)

3D Structural Models

Turn

309..312

Helix

122..124; 157..160; 163..165; 198..203; 210..229; 239..241; 264..271; 272..276; 279..282; 290..305; 315..323; 337..346; 351..353; 357..372; 376..396

Beta Strand

126..134; 137..144; 147..153; 176..180; 187..191; 242..244; 250..252

3D Structure

X-ray crystallography (12)

Domain & Motif Annotations

Compositional Bias

539..551; Basic residues; 572..584; Gly residues; 621..631; Gly residues; 671..692; Gly residues; 719..730; Acidic residues; 732..756; Polar residues; 765..779; Polar residues; 780..790; Basic and acidic residues; 800..816; Pro residues

Domain (CC)

Interacts with MBIP through the leucine-zipper motif.

Domain (FT)

125..366; Protein kinase

Region

51..73; Disordered; 390..411; Leucine-zipper 1; 443..464; Leucine-zipper 2; 524..587; Disordered; 613..859; Disordered

Protein Families (3)

Protein kinase superfamily
STE Ser/Thr protein kinase family
MAP kinase kinase kinase subfamily

Sequence Similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

Clinical Relevance5

Drugs

CEP-1347

Antibody (2)

HPA039936 HPA071996

Interaction Protein

ENSG00000146648

Interaction Count

Interaction Dataset

intact_biogrid

Supporting Publications2

PMID	Title	Abstract
38490958	Proteomic analysis of ascitic extracellular vesicles describes tumour microenvironment and predicts patient survival in ovarian cancer.	No abstract available
38731868	The Deep Proteomics Approach Identified Extracellular Vesicular Proteins Correlated to Extracellular Matrix in Type One and Two Endometrial Cancer.	No abstract available