Protein detail

MKRN3

E3 ubiquitin-protein ligase makorin-3 (EC 2.3.2.27) (RING finger protein 63) (RING-type E3 ubiquitin transferase makorin-3) (Zinc finger protein 127)

Entry name MKRN3	UniProt ID Q13064	EVMP confidence score 0.38
Supporting publications (n) 1	Transmembrane count	Protein classification Disease related genesEnzymesHuman disease related genesMetabolic proteinsPotential drug targetsPredicted intracellular proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

E3 ubiquitin-protein ligase makorin-3 (EC 2.3.2.27) (RING finger protein 63) (RING-type E3 ubiquitin transferase makorin-3) (Zinc finger protein 127)

Protein Class (6)

Disease related genesEnzymesHuman disease related genesMetabolic proteinsPotential drug targetsPredicted intracellular proteins

Protein Function (6)

Predicted intracellular proteins
ENZYME proteins:Transferases
Human disease related genes:Endocrine and metabolic diseases:Hypothalamus and pituitary gland diseases
Potential drug targets
Enzymes
Disease related genes

Ensembl

ENSG00000179455

Entrez Gene Symbol

MKRN3

Gene Synonym (5)

D15S9MGC88288RNF63ZFP127ZNF127

Gene Description

Makorin ring finger protein 3

Chromosome

Position

23565674-23630075

Supporting publications (n)

EVMP confidence score

0.38

Fluorescence & Localization3

Cell SpecificEndometrial ciliated cellsSingle-Nuclei Brain Specificpericyte

Function & Pathway5

Protein Function (6)

Predicted intracellular proteins
ENZYME proteins:Transferases
Human disease related genes:Endocrine and metabolic diseases:Hypothalamus and pituitary gland diseases
Potential drug targets
Enzymes
Disease related genes

Cellular Component

GO:1990904 ribonucleoprotein complex

Molecular Function (5)

Biological Process (3)

Mediation Categories (4)

Clinical-translation mediationFusion and delivery mediationImmune mediationReceptor-signaling mediation

Relations & Evidence148

Ligand-Receptor Signaling (5)

5 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No

Regulatory Interaction Network (137)

137 records.

Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
COMPLEX:P0CG47_P60604	MKRN3	Q13064	Yes	Yes	No	SIGNOR	SIGNOR:34199813
COMPLEX:P62253_P62987	MKRN3	Q13064	Yes	Yes	No	SIGNOR	SIGNOR:34199813
COMPLEX:P62987_Q13404	MKRN3	Q13064	Yes	Yes	No	SIGNOR	SIGNOR:34199813
COMPLEX:P62987_Q16763	MKRN3	Q13064	Yes	Yes	No	SIGNOR	SIGNOR:34199813
COMPLEX:P0CG47_P62256	MKRN3	Q13064	Yes	Yes	No	SIGNOR	SIGNOR:34199813
COMPLEX:P0CG48_Q13404	MKRN3	Q13064	Yes	Yes	No	SIGNOR	SIGNOR:34199813
COMPLEX:P0CG48_Q96B02	MKRN3	Q13064	Yes	Yes	No	SIGNOR	SIGNOR:34199813
COMPLEX:P0CG47_Q5VVX9	MKRN3	Q13064	Yes	Yes	No	SIGNOR	SIGNOR:34199813
COMPLEX:P62987_Q9NPD8	MKRN3	Q13064	Yes	Yes	No	SIGNOR	SIGNOR:34199813
COMPLEX:P62979_P63279	MKRN3	Q13064	Yes	Yes	No	SIGNOR	SIGNOR:34199813

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Protein Complex Composition (5)

5 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
HT_DM_Cluster289	LMNA LMNB1 LMNB2 MKRN1 MKRN2 MKRN3	P02545 P20700 Q03252 Q13064 Q9H000 Q9UHC7	1:1:1:1:1:1	Compleat	Compleat:HC912	22036573
Ub:RING_E3	MKRN3 UBB	P0CG47 Q13064	0:0	SIGNOR	SIGNOR:SIGNOR-C519
Ub:RING_E3	MKRN3 UBC	P0CG48 Q13064	0:0	SIGNOR	SIGNOR:SIGNOR-C519
Ub:RING_E3	MKRN3 RPS27A	P62979 Q13064	0:0	SIGNOR	SIGNOR:SIGNOR-C519
Ub:RING_E3	MKRN3 UBA52	P62987 Q13064	0:0	SIGNOR	SIGNOR:SIGNOR-C519

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential Ultracentrifugation	Mass spectrometry	1	39251709

Sequence, Structure & Domains6

Sequences

Length

507

Mass

55,645

Sequence

MEEPAAPSEAHEAAGAQAGAEAAREGVSGPDLPVCEPSGESAAPDSALPHAARGWAPFPVAPVPAHLRRGGLRPAPASGGGAWPSPLPSRSSGIWTKQIICRYYIHGQCKEGENCRYSHDLSGRKMATEGGVSPPGASAGGGPSTAAHIEPPTQEVAEAPPAASSLSLPVIGSAAERGFFEAERDNADRGAAGGAGVESWADAIEFVPGQPYRGRWVASAPEAPLQSSETERKQMAVGSGLRFCYYASRGVCFRGESCMYLHGDICDMCGLQTLHPMDAAQREEHMRACIEAHEKDMELSFAVQRGMDKVCGICMEVVYEKANPNDRRFGILSNCNHSFCIRCIRRWRSARQFENRIVKSCPQCRVTSELVIPSEFWVEEEEEKQKLIQQYKEAMSNKACRYFAEGRGNCPFGDTCFYKHEYPEGWGDEPPGPGGGSFSAYWHQLVEPVRMGEGNMLYKSIKKELVVLRLASLLFKRFLSLRDELPFSEDQWDLLHYELEEYFNLIL

Domain & Motif Annotations

Compositional Bias

1..21; Low complexity

Zinc Finger

95..122; C3H1-type 1; 238..265; C3H1-type 2; 311..365; RING-type; 394..423; C3H1-type 3

Region

1..48; Disordered; 69..89; Disordered; 126..149; Disordered; 266..293; Makorin-type Cys-His

Clinical Relevance5

Disease Involvement

Disease variant

Antibody

HPA029494

Interaction Protein (4)

ENSG00000072401ENSG00000107929ENSG00000198625ENSG00000229809

Interaction Count

Interaction Dataset (2)

intact_biogridbiogrid_bioplex

Supporting Publications1

PMID	Title	Abstract
34185707	Monocyte-released HERV-K dUTPase engages TLR4 and MCAM causing endothelial mesenchymal transition.	Here we show that monocytes overexpressing HERV-K dUTPase, as opposed to GFP, can release HERV-K dUTPase in extracellular vesicles (EVs) that cause pulmonary hypertension in mice in association with endothelial mesenchymal transition (EndMT) related to induction of SNAIL/SLUG and proinflammatory molecules IL-6 as well as VCAM1.