Protein detail

PAK1

Serine/threonine-protein kinase PAK 1 (EC 2.7.11.1) (Alpha-PAK) (p21-activated kinase 1) (PAK-1) (p65-PAK)

Entry name PAK1	UniProt ID Q13153	EVMP confidence score 0.40
Supporting publications (n) 4	Transmembrane count	Protein classification Disease related genesEnzymesHuman disease related genesPlasma proteinsPotential drug targetsPredicted intracellular proteinsRAS pathway related proteins

Basic Information

Protein Names

Serine/threonine-protein kinase PAK 1 (EC 2.7.11.1) (Alpha-PAK) (p21-activated kinase 1) (PAK-1) (p65-PAK)

Protein Class (7)

Disease related genesEnzymesHuman disease related genesPlasma proteinsPotential drug targetsPredicted intracellular proteinsRAS pathway related proteins

Protein Function (8)

Human disease related genes:Other diseases:Mental and behavioural disorders
Kinases:STE Ser/Thr protein kinases
Predicted intracellular proteins
ENZYME proteins:Transferases
Potential drug targets
RAS pathway related proteins
Enzymes
Disease related genes

Ensembl

ENSG00000149269

Entrez Gene Symbol

PAK1

Gene Description

P21 (RAC1) activated kinase 1

Chromosome

Position

77322017-77474635

Supporting publications (n)

EVMP confidence score

0.40

Fluorescence & Localization

Tissue SpecificliverCell SpecificAstrocytesBlood Cell SpecificneutrophilBlood Lineage Specificgranulocytes

Function & Pathway

Protein Function (8)

Human disease related genes:Other diseases:Mental and behavioural disorders
Kinases:STE Ser/Thr protein kinases
Predicted intracellular proteins
ENZYME proteins:Transferases
Potential drug targets
RAS pathway related proteins
Enzymes
Disease related genes

Cellular Component (18)

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Molecular Function (10)

Biological Process (3)

KEGG (20)

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Reactome (47)

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Mediation Categories (4)

Adhesion and uptake mediationFusion and delivery mediationImmune mediationReceptor-signaling mediation

Relations & Evidence198

Enzyme-Mediated Modification (92)

92 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
PAK1	JAK2	O60674	Y	285	phosphorylation	Sparser_ProtMapperPhosphoSite_MIMPMIMPProtMapperRLIMS-P_ProtMapperREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper	ProtMapper:25472536 ProtMapper:25466889
PAK1	JAK2	O60674	Y	153	phosphorylation	Sparser_ProtMapperPhosphoSite_MIMPMIMPProtMapperRLIMS-P_ProtMapperPhosphoSitePhosphoSite_ProtMapper	ProtMapper:25472536 ProtMapper:26944939 ProtMapper:27542844 ProtMapper:25466889 ProtMapper:17726028
PAK1	JAK2	O60674	Y	201	phosphorylation	PhosphoSite_MIMPMIMPProtMapperRLIMS-P_ProtMapperKEAPhosphoSitePhosphoSite_ProtMapper	ProtMapper:27542844 KEA:17726028 ProtMapper:17726028
PAK1	MAP3K11	Q16584	S	204	phosphorylation	REACH_ProtMapperPhosphoSiteProtMapper	ProtMapper:30664689
PAK1	MAP3K11	Q16584	S	133	phosphorylation	REACH_ProtMapperPhosphoSiteProtMapper	ProtMapper:30664689
PAK1	CDK1	P06493	T	212	phosphorylation	Sparser_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPProtMapperKEAREACH_ProtMapper	ProtMapper:19465939 KEA:11604394 ProtMapper:19165420 ProtMapper:19298660 KEA:17570479 KEA:12176333 KEA:17114649
PAK1	CDK2	P24941	T	212	phosphorylation	MIMPHPRD_MIMPphosphoELM_MIMPPhosphoSite_MIMP
PAK1	CDK2	P24941	S	220	phosphorylation	KEA	KEA:17570479
PAK1	CDK3	Q00526	T	212	phosphorylation	MIMPHPRD_MIMPphosphoELM_MIMPPhosphoSite_MIMP
PAK1	CDK10	Q15131	T	212	phosphorylation	MIMPHPRD_MIMPphosphoELM_MIMPPhosphoSite_MIMP

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Ligand-Receptor Signaling (9)

9 records.

Category	Parent	Database	Receiver
receptor	receptor	OmniPath	Yes
intracellular	intracellular	LOCATE
intracellular	intracellular	ComPPI
intracellular	intracellular	GO_Intercell
intracellular	intracellular	UniProt_location
intracellular	intracellular	OmniPath
plasma_membrane	plasma_membrane	UniProt_location
plasma_membrane	plasma_membrane	OmniPath
receptor	receptor	scConnect	Yes

Regulatory Interaction Network (84)

84 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
PAK1	Q13153	PREX2	Q70Z35	Yes		Yes	iPTMnetSIGNORPhosphoSite_ProtMapperProtMapper	SIGNOR:26438819
PAK1	Q13153	ARAF	P10398	Yes	Yes		KEGG-MEDICUSiPTMnetSIGNORProtMapperSIGNOR_ProtMapper	SIGNOR:15710605 ProtMapper:15710605
	COMPLEX:P21127_P30281	PAK1	Q13153	Yes	Yes		SIGNOR	SIGNOR:19520772
PAK1	Q13153	SNAI1	O95863	Yes	Yes		Sparser_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPiPTMnetSIGNORphosphoELMProtMapperRLIMS-P_ProtMapperPhosphoSite_KEAKEAphosphoELM_KEAInnateDBSIGNOR_ProtMapperREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper	PhosphoSite:28423359 SIGNOR:15833848 ProtMapper:29597073 ProtMapper:31275381 ProtMapper:19923321 ProtMapper:19379710 ProtMapper:19860811 KEA:15833848 ProtMapper:24556840 phosphoELM:15833848 ProtMapper:25122124 ProtMapper:22595609 InnateDB:15833848 ProtMapper:21535025 ProtMapper:25084828 ProtMapper:24168186 ProtMapper:15833848 ProtMapper:26004407 ProtMapper:25472536 PhosphoSite:15833848
PAK1	Q13153	MERL	P35240	Yes	Yes	Yes	HPRD_MIMPSIGNORProtMapperRLIMS-P_ProtMapperHINTPhosphoSite_KEAdbPTMInnateDBHPRDKinexus_KEAWangPhosphoSite_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPiPTMnetPhosphoPointKEAHPRD_KEASIGNOR_ProtMapperREACH_ProtMapperSparser_ProtMapperAdhesomeHPRD-phos	Adhesome:14580336 HPRD-phos:18669648 HPRD-phos:19691289 ProtMapper:27285107 ProtMapper:25893302 ProtMapper:18071304 SIGNOR:18071304 ProtMapper:19165420 HINT:11719502 ProtMapper:23162742 ProtMapper:28090172 KEA:17081983 HPRD-phos:20068231 ProtMapper:20302846 KEA:11782491 dbPTM:18669648 KEA:14981079 KEA:15378014 HPRD:14580336 ProtMapper:22567403 HINT:14580336 dbPTM:17081983 ProtMapper:31652973 HPRD-phos:11719502 ProtMapper:19651622 KEA:11719502 ProtMapper:27345717 ProtMapper:18835652 ProtMapper:11719502 ProtMapper:19691289 ProtMapper:26555075 ProtMapper:22105362 InnateDB:11719502 HPRD:11719502 HINT:21481793 Adhesome:21481793 ProtMapper:19036346 Adhesome:11719502 ProtMapper:20068231 ProtMapper:18669648 HPRD-phos:19651622
PAK1	Q13153	HACE1	Q8IYU2	Yes		Yes	Sparser_ProtMapperiPTMnetSIGNORProtMapperSIGNOR_ProtMapperREACH_ProtMapperPhosphoSite_ProtMapper	SIGNOR:29362425 ProtMapper:30622235 ProtMapper:29362425
CD11A	Q9UQ88	PAK1	Q13153	Yes	Yes		HPRDLit-BM-17SIGNOR	Lit-BM-17:12624090 HPRD:12624090 SIGNOR:19520772
PAK1	Q13153	ESR1	P03372	Yes	Yes		HPRD_MIMPSIGNORProtMapperRLIMS-P_ProtMapperHINTPhosphoSite_KEAphosphoELM_KEAInnateDBPhosphoNetworksHPRDWangPhosphoSite_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPiPTMnetPhosphoPointKEABioGRIDHPRD_KEAphosphoELMSIGNOR_ProtMapperREACH_ProtMapperSparser_ProtMapperHPRD-phos	ProtMapper:20179234 InnateDB:12374744 ProtMapper:24529727 ProtMapper:19557173 ProtMapper:24505617 ProtMapper:16778166 ProtMapper:26554417 HPRD-phos:12374744 ProtMapper:18755239 ProtMapper:15178330 ProtMapper:28978098 ProtMapper:22595609 phosphoELM:12374744 ProtMapper:18521086 BioGRID:16452229 ProtMapper:26944939 ProtMapper:19205871 ProtMapper:21893191 InnateDB:16452229 ProtMapper:27003261 SIGNOR:12374744 KEA:15193262 HINT:12374744 HINT:16452229 HPRD:12374744 ProtMapper:17604944 KEA:12374744 ProtMapper:25447917 ProtMapper:12374744 ProtMapper:22295213
PAK1	Q13153	CENPR	Q13352	Yes	Yes		Sparser_ProtMapperPhosphoSite_MIMPMIMPiPTMnetSIGNORProtMapperRLIMS-P_ProtMapperSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	PhosphoSite:25409762 SIGNOR:18521086 ProtMapper:18521086
PAK1	Q13153	AURKA	O14965	Yes	Yes		Sparser_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetSIGNORProtMapperdbPTMSIGNOR_ProtMapperPhosphoSite_ProtMapper	dbPTM:16246726 ProtMapper:22864622 SIGNOR:24867643 ProtMapper:21589936 ProtMapper:24867643

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Protein Complex Composition (12)

12 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
ARHGEF7-GIT2-PAK1 complex	ARHGEF7 GIT2 PAK1	Q13153 Q14155 Q14161	0:0:0	CORUM	CORUM:6928	11157752
Cdc42p/Ste20p complex	CDC42 PAK1 PAK2 PAK3 PAK6	O75914 P60953 Q13153 Q13177 Q9NQU5	1:1:1:1:1	Compleat	Compleat:HC1807	9003780
GIT1-ARHGEF7-PAK1-PXN complex	ARHGEF7 GIT1 PAK1 PXN	P49023 Q13153 Q14155 Q9Y2X7	0:0:0:0	CORUM	CORUM:6341	16944954
	ACTA1 ACTB CDC42 FHOD1 FMNL2 NRAP PAK1 PFN1 RAC1 TBC1D3F	A6NER0 P07737 P60709 P60953 P63000 P68133 Q13153 Q86VF7 Q96PY5 Q9Y613	1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC7706
	AKT1 AKT2 AKT3 CDC37 CDK11B HSP90AA1 PAK1 PDPK1 PRKCD PRKCI PRKCZ UBC	O15530 P07900 P0CG48 P21127 P31749 P31751 P41743 Q05513 Q05655 Q13153 Q16543 Q9Y243	1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC6406
	CCNB1 CCND3 CDK11B FOXL2 FOXO1 PAK1	P14635 P21127 P30281 P58012 Q12778 Q13153	1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC5826
	CSNK2A1 MAZ PAK1	P56270 P68400 Q13153	1:1:1	CompleatCFinder	Compleat:HC3716
	PAK1 RAC3	P60763 Q13153	1:1	PDB	PDB:2qme
	ARHGEF7 GIT1 GIT2 PAK1 PAK2 YWHAG	P61981 Q13153 Q13177 Q14155 Q14161 Q9Y2X7	1:1:1:1:1:1	CompleatCFinder	Compleat:HC7135
	PAK1	Q13153	4	PDB	PDB:1f3mPDB:5deyPDB:9d4xPDB:9n48PDB:4zjiPDB:4o0tPDB:5kbrPDB:4zy5PDB:4zjjPDB:9nbxPDB:9d4wPDB:9d50PDB:3q4zPDB:9d4vPDB:4zy4PDB:5imePDB:4zy6PDB:5dewPDB:5kbqPDB:6b16PDB:7vtoPDB:4p90PDB:9n4uPDB:4zloPDB:9d4yPDB:4o0r

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Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Protein Organic Solvent Precipitation	R SequencingMass spectrometry	1	32384937

Sequence, Structure & Domains

Sequences

Length

545

Mass

60,647

Sequence

MSNNGLDIQDKPPAPPMRNTSTMIGAGSKDAGTLNHGSKPLPPNPEEKKKKDRFYRSILPGDKTNKKKEKERPEISLPSDFEHTIHVGFDAVTGEFTGMPEQWARLLQTSNITKSEQKKNPQAVLDVLEFYNSKKTSNSQKYMSFTDKSAEDYNSSNALNVKAVSETPAVPPVSEDEDDDDDDATPPPVIAPRPEHTKSVYTRSVIEPLPVTPTRDVATSPISPTENNTTPPDALTRNTEKQKKKPKMSDEEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYTAMDVATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALYLIATNGTPELQNPEKLSAIFRDFLNRCLEMDVEKRGSAKELLQHQFLKIAKPLSSLTPLIAAAKEATKNNH

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q13153-1; Sequence=Displayed; Name=2; Synonyms=PAK1B; IsoId=Q13153-2; Sequence=VSP_017507

Alternative Sequence

518..545; HQFLKIAKPLSSLTPLIAAAKEATKNNH -> VRKLRFQVFSNFSMIAASIPEDCQAPLQPHSTDCCS (in isoform 2)

3D Structural Models

Turn

91..94; 290..292; 463..466; 506..508

Helix

101..108; 114..119; 121..135; 250..260; 266..268; 303..305; 309..321; 352..358; 363..382; 392..394; 428..430; 433..437; 444..459; 469..479; 487..489; 492..501; 512..515; 519..523; 527..530; 531..540

Beta Strand

79..87; 95..98; 195..198; 214..219; 261..264; 270..272; 274..279; 282..289; 295..302; 330..336; 339..345; 349..351; 395..397; 403..405; 408..410; 416..418

3D Structure

NMR spectroscopy (1); X-ray crystallography (36)

Domain & Motif Annotations

Compositional Bias

68..77; Basic and acidic residues; 174..184; Acidic residues; 220..231; Polar residues

Domain (FT)

75..88; CRIB; 270..521; Protein kinase

Region

1..77; Disordered; 70..140; Autoregulatory region; 75..105; GTPase-binding; 159..198; Disordered; 211..251; Disordered

Protein Families (3)

Protein kinase superfamily
STE Ser/Thr protein kinase family
STE20 subfamily

Sequence Similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Clinical Relevance

Disease Involvement (3)

Disease variantEpilepsyIntellectual disability

Related Diseases (2)

Epilepsy Intellectual disability

Drugs (10)

CENISERTIB PF-562271 BARASERTIB-HQPA NVP-TAE684 MLN-8054 LAUROGUADINE TOZASERTIB ILORASERTIB TAMATINIB RG-1530

Antibody (2)

HPA003565 CAB005312

Interaction Protein (12)

ENSG00000070831ENSG00000071051ENSG00000088986ENSG00000102606ENSG00000106683ENSG00000108262ENSG00000116574ENSG00000126785ENSG00000129675ENSG00000136238ENSG00000139436ENSG00000146648

Interaction Count

Interaction Dataset (2)

intact_biogridintact_biogrid_opencell

Supporting Publications4

PMID	Title	Related sentences
30301952	A position on vision.	No related sentences available
38343172	Analysis of secreted small extracellular vesicles from activated human microglial cell lines reveals distinct pro- and anti-inflammatory proteomic profiles.	No related sentences available
40222457	Proteome alterations in peripheral immune cells of DLBCL patients and evidence of cancer extracellular vesicles involvement.	No related sentences available
40595564	Enrichment of extracellular vesicles using Mag-Net for the analysis of the plasma proteome.	No related sentences available