Protein detail
PAK1
Serine/threonine-protein kinase PAK 1 (EC 2.7.11.1) (Alpha-PAK) (p21-activated kinase 1) (PAK-1) (p65-PAK)
Entry name PAK1 | UniProt ID | EVMP confidence score 0.40 |
Supporting publications (n) 4 | Transmembrane count | Protein classification Disease related genesEnzymesHuman disease related genesPlasma proteinsPotential drug targetsPredicted intracellular proteinsRAS pathway related proteins |
Annotation confidence score; open for threshold definitions.
Extremely high >= 0.70High >= 0.60Medium >= 0.40Low >= 0.30Basic Information
Protein Names
Serine/threonine-protein kinase PAK 1 (EC 2.7.11.1) (Alpha-PAK) (p21-activated kinase 1) (PAK-1) (p65-PAK)
Protein Class (7)
Disease related genesEnzymesHuman disease related genesPlasma proteinsPotential drug targetsPredicted intracellular proteinsRAS pathway related proteins
Protein Function (8)
- Human disease related genes:Other diseases:Mental and behavioural disorders
- Kinases:STE Ser/Thr protein kinases
- Predicted intracellular proteins
- ENZYME proteins:Transferases
- Potential drug targets
- RAS pathway related proteins
- Enzymes
- Disease related genes
Ensembl
Entrez Gene Symbol
Gene Description
P21 (RAC1) activated kinase 1
Chromosome
11
Position
77322017-77474635
Supporting publications (n)
4
EVMP confidence score
0.40
Fluorescence & Localization
Tissue SpecificliverCell SpecificAstrocytesBlood Cell SpecificneutrophilBlood Lineage Specificgranulocytes
Function & Pathway
Protein Function (8)
- Human disease related genes:Other diseases:Mental and behavioural disorders
- Kinases:STE Ser/Thr protein kinases
- Predicted intracellular proteins
- ENZYME proteins:Transferases
- Potential drug targets
- RAS pathway related proteins
- Enzymes
- Disease related genes
Cellular Component (18)
- GO:0001726 ruffle
- GO:0005654 nucleoplasm
- GO:0005694 chromosome
- GO:0005737 cytoplasm
- GO:0005813 centrosome
- GO:0005829 cytosol
- GO:0005884 actin filament
- GO:0005886 plasma membrane
- GO:0005911 cell-cell junction
- GO:0005925 focal adhesion
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Molecular Function (10)
- GO:0004672 protein kinase activity
- GO:0004674 protein serine/threonine kinase activity
- GO:0005515 protein binding
- GO:0005518 collagen binding
- GO:0005524 ATP binding
- GO:0031267 small GTPase binding
- GO:0042802 identical protein binding
- GO:0043015 gamma-tubulin binding
- GO:0044024 histone H2AS1 kinase activity
- GO:0106310 protein serine kinase activity
Biological Process (3)
KEGG (20)
- hsa04010 MAPK signaling pathway
- KEGG:hsa04012 ErbB signaling pathway
- KEGG:hsa04014 Ras signaling pathway
- KEGG:hsa04024 cAMP signaling pathway
- KEGG:hsa04062 Chemokine signaling pathway
- KEGG:hsa04360 Axon guidance
- KEGG:hsa04392 Hippo signaling pathway - multiple species
- KEGG:hsa04510 Focal adhesion
- KEGG:hsa04517 IgSF CAM signaling
- KEGG:hsa04625 C-type lectin receptor signaling pathway
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Reactome (47)
- R-hsa-428540 activation of rac1
- R-hsa-1280218 adaptive immune system
- R-hsa-5621575 cd209 dc sign signaling
- R-hsa-389359 cd28 dependent vav1 pathway
- R-hsa-9013148 cdc42 gtpase cycle
- R-hsa-389356 co stimulation by cd28
- R-hsa-5621481 c type lectin receptors clrs
- R-hsa-3928662 ephb mediated forward signaling
- R-hsa-3928664 ephrin signaling
- R-hsa-2682334 eph ephrin signaling
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Mediation Categories (4)
Adhesion and uptake mediationFusion and delivery mediationImmune mediationReceptor-signaling mediation
Relations & Evidence198
Enzyme-Mediated Modification (92)
92 records.
| Substrate Gene Symbol | Enzyme Gene Symbol | Enzyme UniProt ID | Residue Type | Residue Offset | Modification | Database | References |
|---|---|---|---|---|---|---|---|
| PAK1 | CSF1 | P09603 | T | 423 | phosphorylation | REACH_ProtMapperSparser_ProtMapperProtMapper | ProtMapper:18940914ProtMapper:18668521 |
| PAK1 | IL1B | P01584 | T | 423 | phosphorylation | REACH_ProtMapperSparser_ProtMapperProtMapper | ProtMapper:26036343 |
| PAK1 | IFNG | P01579 | T | 423 | phosphorylation | REACH_ProtMapperSparser_ProtMapperProtMapper | ProtMapper:26036343 |
| PAK1 | CDC42 | P60953 | T | 423 | phosphorylation | REACH_ProtMapperProtMapper | ProtMapper:19812368 |
| PAK1 | CDC42 | P60953 | S | 204 | phosphorylation | NCI-PID_ProtMapperProtMapper | ProtMapper:19628037ProtMapper:15788770 |
| PAK1 | CDC42 | P60953 | S | 199 | phosphorylation | NCI-PID_ProtMapperProtMapper | ProtMapper:19628037ProtMapper:15788770 |
| PAK1 | BRSK2 | Q8IWQ3 | T | 423 | phosphorylation | RLIMS-P_ProtMapperSparser_ProtMapperProtMapper | ProtMapper:22669945 |
| PAK1 | F2 | P00734 | T | 423 | phosphorylation | Sparser_ProtMapperProtMapper | ProtMapper:19359598 |
| PAK1 | F2 | P00734 | S | 144 | phosphorylation | Sparser_ProtMapperProtMapper | ProtMapper:19359598 |
| PAK1 | TNF | P01375 | T | 423 | phosphorylation | Sparser_ProtMapperProtMapper | ProtMapper:26036343 |
Ligand-Receptor Signaling (9)
9 records.
| Category | Parent | Database | Transmitter | Receiver | Secreted | Plasma Membrane (Transmembrane) | Plasma Membrane (Peripheral) |
|---|---|---|---|---|---|---|---|
| receptor | receptor | OmniPath | Yes | ||||
| intracellular | intracellular | LOCATE | |||||
| intracellular | intracellular | ComPPI | |||||
| intracellular | intracellular | GO_Intercell | |||||
| intracellular | intracellular | UniProt_location | |||||
| intracellular | intracellular | OmniPath | |||||
| plasma_membrane | plasma_membrane | UniProt_location | |||||
| plasma_membrane | plasma_membrane | OmniPath | |||||
| receptor | receptor | scConnect | Yes |
Regulatory Interaction Network (84)
84 records.
| Source Protein Symbol | Source UniProt ID | Target Protein Symbol | Target UniProt ID | Is Directed | Is Stimulation | Is Inhibition | Database | References |
|---|---|---|---|---|---|---|---|---|
| CDK19 | Q9BWU1 | PAK1 | Q13153 | Yes | phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetSIGNORProtMapperSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper | SIGNOR:19520772ProtMapper:19520772PhosphoSite:19520772 | ||
| VIME | P08670 | PAK1 | Q13153 | Yes | Yes | SIGNORAdhesome | Adhesome:11895474SIGNOR:15766329 | |
| PAK1 | Q13153 | VIME | P08670 | Yes | Yes | WangPhosphoNetworksSparser_ProtMapperphosphoELM_MIMPAdhesomeMIMPPhosphoSite_MIMPHPRD_MIMPiPTMnetSIGNORProtMapperPhosphoSite_KEAKEAphosphoELM_KEASIGNOR_ProtMapperREACH_ProtMapperPhosphoSite_ProtMapper | KEA:14722118ProtMapper:28390425ProtMapper:27457922ProtMapper:28096473ProtMapper:26369929KEA:15766329ProtMapper:15766329KEA:12761892KEA:17081983KEA:11895474ProtMapper:26956613KEA:16964243Adhesome:11895474KEA:15345747KEA:11420699KEA:7983050KEA:12458200KEA:16565220SIGNOR:15766329ProtMapper:32328567KEA:15713670 | |
| PAK1 | Q13153 | PA2G4 | Q9UQ80 | Yes | Yes | Sparser_ProtMapperPhosphoSite_MIMPMIMPiPTMnetSIGNORProtMapperRLIMS-P_ProtMapperSIGNOR_ProtMapperREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper | PhosphoSite:18283314ProtMapper:23361053ProtMapper:19557173ProtMapper:18283314ProtMapper:20159994SIGNOR:18283314 | |
| PAK1 | Q13153 | ATG5 | Q9H1Y0 | Yes | Yes | Sparser_ProtMapperSIGNORProtMapperSPIKE_LCREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper | ProtMapper:33796531ProtMapper:32186433SPIKE_LC:17145710PhosphoSite:32186433SIGNOR:36528756 | |
| PAK1 | Q13153 | GIT1 | Q9Y2X7 | Yes | WangSparser_ProtMapperphosphoELM_MIMPAdhesomeMIMPPhosphoSite_MIMPPhosphoSite_norefiPTMnetProtMapperHINTIntActSPIKE_LCREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper | PhosphoSite:16797488ProtMapper:27012601SPIKE_LC:16628223HINT:16628223Adhesome:16628223HINT:25852190ProtMapper:17467235Adhesome:12006652IntAct:16628223 | ||
| PAK1 | Q13153 | GDIB | P50395 | Yes | PhosphoSite_norefPhosphoSitePhosphoSite_ProtMapperProtMapper | PhosphoSite:33914026 | ||
| PAK1 | Q13153 | PCBP1 | Q15365 | Yes | PhosphoSite_MIMPMIMPPhosphoSite_norefiPTMnetProtMapperIntActDIPREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper | DIP:17389360PhosphoSite:17389360ProtMapper:28007610IntAct:17389360 | ||
| PAK1 | Q13153 | MYL9 | P24844 | Yes | phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetProtMapperReactome_ProtMapperPhosphoSitePhosphoSite_ProtMapper | PhosphoSite:21336303PhosphoSite:10704379PhosphoSite:15695393PhosphoSite:26152729PhosphoSite:11673466PhosphoSite:10092231PhosphoSite:17895359PhosphoSite:24610576PhosphoSite:34532864PhosphoSite:3839510PhosphoSite:22137581PhosphoSite:12081505 | ||
| JAK2 | O60674 | PAK1 | Q13153 | Yes | Sparser_ProtMapperPhosphoSite_MIMPMIMPPhosphoSite_norefiPTMnetProtMapperRLIMS-P_ProtMapperKinexus_KEAKEAREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper | PhosphoSite:21719533ProtMapper:25472536ProtMapper:26944939ProtMapper:27542844ProtMapper:25466889PhosphoSite:21566085KEA:17726028ProtMapper:17726028PhosphoSite:17726028PhosphoSite:25125660 |
Protein Complex Composition (12)
12 records.
| Component Name | Component Gene Symbols | Component UniProt ID | Stoichiometry | Database | Database IDs | References |
|---|---|---|---|---|---|---|
| ARHGEF7-GIT2-PAK1 complex | ARHGEF7GIT2PAK1 | Q13153Q14155Q14161 | 0:0:0 | CORUM | CORUM:6928 | 11157752 |
| Cdc42p/Ste20p complex | CDC42PAK1PAK2PAK3PAK6 | O75914P60953Q13153Q13177Q9NQU5 | 1:1:1:1:1 | Compleat | Compleat:HC1807 | 9003780 |
| GIT1-ARHGEF7-PAK1-PXN complex | ARHGEF7GIT1PAK1PXN | P49023Q13153Q14155Q9Y2X7 | 0:0:0:0 | CORUM | CORUM:6341 | 16944954 |
| ACTA1ACTBCDC42FHOD1FMNL2NRAPPAK1PFN1RAC1TBC1D3F | A6NER0P07737P60709P60953P63000P68133Q13153Q86VF7Q96PY5Q9Y613 | 1:1:1:1:1:1:1:1:1:1 | NetworkBlastCompleat | Compleat:HC7706 | ||
| AKT1AKT2AKT3CDC37CDK11BHSP90AA1PAK1PDPK1PRKCDPRKCIPRKCZUBC | O15530P07900P0CG48P21127P31749P31751P41743Q05513Q05655Q13153Q16543Q9Y243 | 1:1:1:1:1:1:1:1:1:1:1:1 | NetworkBlastCompleat | Compleat:HC6406 | ||
| CCNB1CCND3CDK11BFOXL2FOXO1PAK1 | P14635P21127P30281P58012Q12778Q13153 | 1:1:1:1:1:1 | NetworkBlastCompleat | Compleat:HC5826 | ||
| CSNK2A1MAZPAK1 | P56270P68400Q13153 | 1:1:1 | CompleatCFinder | Compleat:HC3716 | ||
| PAK1RAC3 | P60763Q13153 | 1:1 | PDB | PDB:2qme | ||
| ARHGEF7GIT1GIT2PAK1PAK2YWHAG | P61981Q13153Q13177Q14155Q14161Q9Y2X7 | 1:1:1:1:1:1 | CompleatCFinder | Compleat:HC7135 | ||
| PAK1 | Q13153 | 4 | PDB | PDB:1f3mPDB:5deyPDB:9d4xPDB:9n48PDB:4zjiPDB:4o0tPDB:5kbrPDB:4zy5PDB:4zjjPDB:9nbxPDB:9d4wPDB:9d50PDB:3q4zPDB:9d4vPDB:4zy4PDB:5imePDB:4zy6PDB:5dewPDB:5kbqPDB:6b16PDB:7vtoPDB:4p90PDB:9n4uPDB:4zloPDB:9d4yPDB:4o0r |
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Isolation & Detection Technology (1)
1 record.
| EV Isolation Method | Detection Method | Number of References | References |
|---|---|---|---|
| Protein Organic Solvent Precipitation | R SequencingMass spectrometry | 1 | 32384937 |
Sequence, Structure & Domains
Sequences
Length
545
Mass
60,647
Sequence
MSNNGLDIQDKPPAPPMRNTSTMIGAGSKDAGTLNHGSKPLPPNPEEKKKKDRFYRSILPGDKTNKKKEKERPEISLPSDFEHTIHVGFDAVTGEFTGMPEQWARLLQTSNITKSEQKKNPQAVLDVLEFYNSKKTSNSQKYMSFTDKSAEDYNSSNALNVKAVSETPAVPPVSEDEDDDDDDATPPPVIAPRPEHTKSVYTRSVIEPLPVTPTRDVATSPISPTENNTTPPDALTRNTEKQKKKPKMSDEEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYTAMDVATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALYLIATNGTPELQNPEKLSAIFRDFLNRCLEMDVEKRGSAKELLQHQFLKIAKPLSSLTPLIAAAKEATKNNH
Alternative Products
Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q13153-1; Sequence=Displayed; Name=2; Synonyms=PAK1B; IsoId=Q13153-2; Sequence=VSP_017507
Alternative Sequence
518..545; HQFLKIAKPLSSLTPLIAAAKEATKNNH -> VRKLRFQVFSNFSMIAASIPEDCQAPLQPHSTDCCS (in isoform 2)
3D Structural Models
Turn
91..94; 290..292; 463..466; 506..508
Helix
101..108; 114..119; 121..135; 250..260; 266..268; 303..305; 309..321; 352..358; 363..382; 392..394; 428..430; 433..437; 444..459; 469..479; 487..489; 492..501; 512..515; 519..523; 527..530; 531..540
Beta Strand
79..87; 95..98; 195..198; 214..219; 261..264; 270..272; 274..279; 282..289; 295..302; 330..336; 339..345; 349..351; 395..397; 403..405; 408..410; 416..418
3D Structure
NMR spectroscopy (1); X-ray crystallography (36)
Domain & Motif Annotations
Compositional Bias
68..77; Basic and acidic residues; 174..184; Acidic residues; 220..231; Polar residues
Domain (FT)
75..88; CRIB; 270..521; Protein kinase
Region
1..77; Disordered; 70..140; Autoregulatory region; 75..105; GTPase-binding; 159..198; Disordered; 211..251; Disordered
Protein Families (3)
- Protein kinase superfamily
- STE Ser/Thr protein kinase family
- STE20 subfamily
Sequence Similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Clinical Relevance
Disease Involvement (3)
Disease variantEpilepsyIntellectual disability
Related Diseases (2)
Drugs (10)
Interaction Protein (12)
ENSG00000070831ENSG00000071051ENSG00000088986ENSG00000102606ENSG00000106683ENSG00000108262ENSG00000116574ENSG00000126785ENSG00000129675ENSG00000136238ENSG00000139436ENSG00000146648
Interaction Count
12
Interaction Dataset (2)
intact_biogridintact_biogrid_opencell
Supporting Publications4
| PMID | Title | Related sentences |
|---|---|---|
| 30301952 | A position on vision. | No related sentences available |
| 38343172 | Analysis of secreted small extracellular vesicles from activated human microglial cell lines reveals distinct pro- and anti-inflammatory proteomic profiles. | No related sentences available |
| 40222457 | Proteome alterations in peripheral immune cells of DLBCL patients and evidence of cancer extracellular vesicles involvement. | No related sentences available |
| 40595564 | Enrichment of extracellular vesicles using Mag-Net for the analysis of the plasma proteome. | No related sentences available |