Protein detail
SLAF1
Signaling lymphocytic activation molecule (CDw150) (IPO-3) (SLAM family member 1) (CD antigen CD150)
Entry name SLAF1 | UniProt ID | EVMP confidence score 0.25 |
Supporting publications (n) 1 | Transmembrane count 1 | Protein classification CD markersPredicted membrane proteinsPredicted secreted proteins |
EVMP confidence score
Annotation confidence score; open for threshold definitions.
Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40Basic Information13
Protein Names
Signaling lymphocytic activation molecule (CDw150) (IPO-3) (SLAM family member 1) (CD antigen CD150)
Protein Class (3)
CD markersPredicted membrane proteinsPredicted secreted proteins
Protein Function (2)
- Predicted secreted proteins
- CD markers
Transmembrane
238..258; Helical
Transmembrane Count
1
Ensembl
Entrez Gene Symbol
Gene Synonym (2)
CD150SLAM
Gene Description
Signaling lymphocytic activation molecule family member 1
Chromosome
1
Position
160608106-160647044
Supporting publications (n)
1
EVMP confidence score
0.25
Fluorescence & Localization2
Tissue SpecificesophagusCell SpecificAlveolar cells type 1
Function & Pathway6
Protein Function (2)
- Predicted secreted proteins
- CD markers
Cellular Component (4)
Molecular Function (8)
Biological Process (3)
KEGG (3)
Mediation Categories (4)
Adhesion and uptake mediationFusion and delivery mediationImmune mediationReceptor-signaling mediation
Relations & Evidence63
Enzyme-Mediated Modification (9)
9 records.
| Substrate Gene Symbol | Enzyme Gene Symbol | Enzyme UniProt ID | Residue Type | Residue Offset | Modification | Database | References |
|---|---|---|---|---|---|---|---|
| SLAMF1 | FYN | P06241 | Y | 281 | phosphorylation | PhosphoNetworksphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperHPRDKEAphosphoELMSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper | HPRD:11806999KEA:11806999phosphoELM:11806999ProtMapper:11806999SIGNOR:11806999 |
| SLAMF1 | FYN | P06241 | Y | 307 | phosphorylation | PhosphoNetworksphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperHPRDKEAphosphoELMSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper | HPRD:11806999KEA:11806999phosphoELM:11806999ProtMapper:11806999SIGNOR:11806999 |
| SLAMF1 | FYN | P06241 | Y | 327 | phosphorylation | PhosphoNetworksphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperHPRDKEAphosphoELMSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper | HPRD:11806999KEA:11806999phosphoELM:11806999KEA:15315965ProtMapper:11806999SIGNOR:11806999 |
| SLAMF1 | LCK | P06239 | Y | 307 | phosphorylation | phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPProtMapperKEAphosphoELMPhosphoSitePhosphoSite_ProtMapper | phosphoELM:11806999KEA:11806999 |
| SLAMF1 | LYN | P07948 | Y | 327 | phosphorylation | BEL-Large-Corpus_ProtMapperPhosphoNetworksSparser_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperKEAphosphoELMSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper | ProtMapper:15212693SIGNOR:15315965KEA:11806999phosphoELM:15315965KEA:15315965ProtMapper:15315965 |
| SLAMF1 | LYN | P07948 | Y | 281 | phosphorylation | PhosphoNetworks | |
| SLAMF1 | SRC | P12931 | Y | 327 | phosphorylation | Li2012 | |
| SLAMF1 | SRC | P12931 | Y | 281 | phosphorylation | Li2012 | |
| SLAMF1 | SRC | P12931 | Y | 307 | phosphorylation | Li2012 |
Ligand-Receptor Signaling (46)
46 records.
| Category | Parent | Database | Transmitter | Receiver | Secreted | Plasma Membrane (Transmembrane) | Plasma Membrane (Peripheral) |
|---|---|---|---|---|---|---|---|
| receptor | receptor | connectomeDB2020 | No | Yes | Yes | Yes | No |
| receptor | receptor | CellCellInteractions | No | Yes | Yes | Yes | No |
| transmembrane | transmembrane_predicted | Phobius | No | No | Yes | Yes | No |
| transmembrane_phobius | transmembrane_predicted | Almen2009 | No | No | Yes | Yes | No |
| transmembrane_sosui | transmembrane_predicted | Almen2009 | No | No | Yes | Yes | No |
| transmembrane_tmhmm | transmembrane_predicted | Almen2009 | No | No | Yes | Yes | No |
Page 5 of 5Previous
Regulatory Interaction Network (4)
4 records.
| Source Protein Symbol | Source UniProt ID | Target Protein Symbol | Target UniProt ID | Is Directed | Is Stimulation | Is Inhibition | Database | References |
|---|---|---|---|---|---|---|---|---|
| FYN | P06241 | SLAF1 | Q13291 | Yes | Yes | No | HPRD_MIMPSIGNORProtMapperPhosphoSite_KEAphosphoELM_KEAPhosphoNetworksHPRDWangPhosphoSite_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPPhosphoSite_norefPhosphoPointiPTMnetKEAHPRD_KEAphosphoELMSIGNOR_ProtMapperSPIKE_LCHPRD-phos | HPRD:12458214HPRD-phos:11806999HPRD:11806999KEA:11806999phosphoELM:11806999KEA:15315965ProtMapper:11806999SPIKE_LC:16713569SIGNOR:11806999 |
| MARH9 | Q86YJ5 | SLAF1 | Q13291 | Yes | No | Yes | SIGNOR | SIGNOR:19457934 |
| LYN | P07948 | SLAF1 | Q13291 | Yes | Yes | No | WangBEL-Large-Corpus_ProtMapperPhosphoNetworksSparser_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPPhosphoSite_norefSIGNORiPTMnetProtMapperPhosphoSite_KEAKEAphosphoELM_KEAphosphoELMSIGNOR_ProtMapperPhosphoSite_ProtMapper | ProtMapper:15212693SIGNOR:15315965KEA:11806999phosphoELM:15315965KEA:15315965ProtMapper:15315965 |
| LCK | P06239 | SLAF1 | Q13291 | Yes | Yes | No | phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetProtMapperPhosphoSite_KEAKEAphosphoELM_KEAphosphoELMWangPhosphoSitePhosphoSite_ProtMapper | phosphoELM:11806999KEA:11806999PhosphoSite:11806999 |
Protein Complex Composition (3)
3 records.
| Component Name | Component Gene Symbols | Component UniProt ID | Stoichiometry | Database | Database IDs | References |
|---|---|---|---|---|---|---|
| Slam-SAP complex | SH2D1ASLAMF1 | O60880Q13291 | 2:2 | CompleatPDB | PDB:1ka7Compleat:HC2828PDB:1d4tPDB:1d4wPDB:1ka6 | 11477403 |
| Slam-SAP-SHIP complex | INPP5DSH2D1ASLAMF1 | O60880Q13291Q92835 | 1:1:1 | Compleat | Compleat:HC728 | 11477403 |
| GK2NKIRAS1SLAMF1 | Q13291Q14410Q9NYS0 | 0:0:0 | hu.MAP2 |
Isolation & Detection Technology (1)
1 record.
| EV Isolation Method | Detection Method | Number of References | References |
|---|---|---|---|
| Protein Organic Solvent Precipitation | R SequencingMass spectrometry | 1 | 32384937 |
Sequence, Structure & Domains10
Sequences
Length
335
Mass
37,231
Sequence
MDPKGLLSLTFVLFLSLAFGASYGTGGRMMNCPKILRQLGSKVLLPLTYERINKSMNKSIHIVVTMAKSLENSVENKIVSLDPSEAGPPRYLGDRYKFYLENLTLGIRESRKEDEGWYLMTLEKNVSVQRFCLQLRLYEQVSTPEIKVLNKTQENGTCTLILGCTVEKGDHVAYSWSEKAGTHPLNPANSSHLLSLTLGPQHADNIYICTVSNPISNNSQTFSPWPGCRTDPSETKPWAVYAGLLGGVIMILIMVVILQLRRRGKTNHYQTTVEKKSLTIYAQVQKPGPLQKKLDSFPAQDPCTTIYVAATEPVPESVQETNSITVYASVTLPES
Alternative Products
Event=Alternative splicing; Named isoforms=4; Comment=Additional isoforms seem to exist.; Name=1; Synonyms=Long, mCD150; IsoId=Q13291-1; Sequence=Displayed; Name=2; Synonyms=Short, SURslam2, vmSLAM; IsoId=Q13291-2; Sequence=VSP_002568, VSP_002569; Name=3; Synonyms=Secreted, SECslam, sSLAM; IsoId=Q13291-3; Sequence=VSP_002567; Name=4; Synonyms=nCD150; IsoId=Q13291-4; Sequence=VSP_058033
Alternative Sequence
234..263; Missing (in isoform 3); 264..335; GKTNHYQTTVEKKSLTIYAQVQKPGPLQKKLDSFPAQDPCTTIYVAATEPVPESVQETNSITVYASVTLPES -> ATLTTTNQYWSQNVLTQDQERCPGCLPMVKRTITRQQWKKKALRSMPKSRNQVLFRRNLTPSQLRTLAPPYMLLPQSLSQSLSRKQIPSQSMLV (in isoform 4); 289..298; PLQKKLDSFP -> DTHHQTSDLF (in isoform 2); 299..335; Missing (in isoform 2)
3D Structural Models
Beta Strand
278..281
3D Structure
NMR spectroscopy (2); X-ray crystallography (4)
Domain & Motif Annotations
Motif
279..284; ITSM 1; 307..312; SH2-binding; 325..330; ITSM 2
Domain (CC)
The ITSMs (immunoreceptor tyrosine-based switch motifs) with the consensus sequence T-X-Y-X-X-[VI] present in SLAM family receptors have overlapping specificity for activating and inhibitory SH2 domain-containing binding partners. Especially they mediate the interaction with the SH2 domain of SH2D1A and SH2D1B. For SLAMF1 a 'two-out-of-three-pronged' mechanism is proposed involving threonine (position -2), phosphorylated tyrosine (position 0) and valine/isoleucine (position +3). SH2D1A binding is mediated by either three 'prongs' (for high affinity binding involving ITSM 1) or a combination of any two also including non-phosphorylated Tyr-281 of ITSM 1 thus providing a positive feedback loop implicating SH2D1A-dependent recruitment of activating FYN. ITSM 2 needs to be phosphorylated on Tyr-327 for SH2D1A binding.
Domain (FT)
29..138; Ig-like V-type; 144..223; Ig-like C2-type
Clinical Relevance5
Drugs
Interaction Protein
ENSG00000183918
Interaction Count
1
Interaction Dataset
intact_biogrid
Supporting Publications1
| PMID | Title | Abstract |
|---|---|---|
| 27894104 | Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers. | No abstract available |