Protein detail
STIM1
Stromal interaction molecule 1
Entry name STIM1 | UniProt ID | EVMP score 0.47 |
Frequency 15 | Transmembrane count 1 | Protein classification Disease related genesHuman disease related genesPlasma proteinsPotential drug targetsPredicted intracellular proteinsPredicted membrane proteinsTransporters |
EVMP score: annotation confidence score.
Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40
Basic Information
Protein Names
Stromal interaction molecule 1
Protein Class
Disease related genesHuman disease related genesPlasma proteinsPotential drug targetsPredicted intracellular proteinsPredicted membrane proteinsTransporters
Protein Function
- Human disease related genes:Cardiovascular diseases:Hematologic diseases
- Predicted intracellular proteins
- Potential drug targets
- Human disease related genes:Musculoskeletal diseases:Muscular diseases
- Human disease related genes:Immune system diseases:Primary immunodeficiency
- Transporters:Transporter channels and pores
- Disease related genes
Transmembrane
214..234; Helical
Transmembrane Count
1
Ensembl
Entrez Gene Symbol
Gene Synonym
D11S4896EGOK
Gene Description
Stromal interaction molecule 1
Chromosome
11
Position
3854527-4093210
Frequency
15
EVMP Score
0.47
Fluorescence & Localization
Tissue SpecificliverCell SpecificBreast lactating cellsSingle-Nuclei Brain SpecificBergmann gliaBlood Cell SpecificneutrophilBlood Lineage Specificgranulocytes
Function & Pathway
Protein Function
- Human disease related genes:Cardiovascular diseases:Hematologic diseases
- Predicted intracellular proteins
- Potential drug targets
- Human disease related genes:Musculoskeletal diseases:Muscular diseases
- Human disease related genes:Immune system diseases:Primary immunodeficiency
- Transporters:Transporter channels and pores
- Disease related genes
Cellular Component
Molecular Function
Biological Process
Reactome
- R-hsa-1280218 adaptive immune system
- R-hsa-983695 antigen activates b cell receptor bcr leading to generation of second messengers
- R-hsa-5576891 cardiac conduction
- R-hsa-139853 elevation of cytosolic ca2 levels
- R-hsa-109582 hemostasis
- R-hsa-5578775 ion homeostasis
- R-hsa-397014 muscle contraction
- R-hsa-418360 platelet calcium homeostasis
- R-hsa-418346 platelet homeostasis
- R-hsa-983705 signaling by the b cell receptor bcr
Mediation Categories
Fusion and delivery mediationImmune mediationMetabolism mediationReceptor-signaling mediation
Relations & Evidence
Enzyme-Mediated Modification
20 records.
| Substrate Gene Symbol | Enzyme Gene Symbol | Enzyme UniProt ID | Residue Type | Residue Offset | Modification | Database | References |
|---|---|---|---|---|---|---|---|
| STIM1 | PRKAB1 | Q9Y478 | S | 521 | phosphorylation | PhosphoSite | |
| STIM1 | PRKAB1 | Q9Y478 | S | 257 | phosphorylation | PhosphoSite | |
| STIM1 | EGF | P01133 | S | 575 | phosphorylation | REACH_ProtMapperProtMapper | ProtMapper:25447552 |
| STIM1 | EGF | P01133 | S | 608 | phosphorylation | REACH_ProtMapperProtMapper | ProtMapper:25447552 |
| STIM1 | EGF | P01133 | S | 621 | phosphorylation | REACH_ProtMapperProtMapper | ProtMapper:25447552 |
| STIM1 | HRAS | P01112 | S | 575 | phosphorylation | REACH_ProtMapperProtMapper | ProtMapper:25447552 |
| STIM1 | HRAS | P01112 | S | 608 | phosphorylation | REACH_ProtMapperProtMapper | ProtMapper:25447552 |
| STIM1 | HRAS | P01112 | S | 621 | phosphorylation | REACH_ProtMapperProtMapper | ProtMapper:25447552 |
| STIM1 | NFATC3 | Q12968 | S | 575 | phosphorylation | REACH_ProtMapperProtMapper | ProtMapper:22298426 |
| STIM1 | ESR1 | P03372 | Y | 361 | phosphorylation | REACH_ProtMapperProtMapper | ProtMapper:28218251 |
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Ligand-Receptor Signaling
24 records.
| Category | Parent | Database | Transmitter | Receiver | Secreted | Plasma Membrane (Transmembrane) | Plasma Membrane (Peripheral) |
|---|---|---|---|---|---|---|---|
| transmembrane | transmembrane | TopDB | No | No | No | Yes | No |
| transmembrane | transmembrane | LOCATE | No | No | No | Yes | No |
| transmembrane | transmembrane | Ramilowski_location | No | No | No | Yes | No |
| transmembrane | transmembrane | OmniPath | No | No | No | Yes | No |
| plasma_membrane | plasma_membrane | UniProt_location | No | No | No | Yes | No |
| plasma_membrane | plasma_membrane | OmniPath | No | No | No | Yes | No |
| plasma_membrane_transmembrane | plasma_membrane_transmembrane | CSPA | No | No | No | Yes | No |
| plasma_membrane_transmembrane | plasma_membrane_transmembrane | OmniPath | No | No | No | Yes | No |
| cell_surface | cell_surface | Surfaceome | No | No | No | Yes | No |
| cell_surface | cell_surface | OmniPath | No | No | No | Yes | No |
Regulatory Interaction Network
9 records.
| Source Protein Symbol | Source UniProt ID | Target Protein Symbol | Target UniProt ID | Is Directed | Is Stimulation | Is Inhibition | Database | References |
|---|---|---|---|---|---|---|---|---|
| FA20C | Q8IXL6 | STIM1 | Q13586 | Yes | Yes | No | SIGNORSparser_ProtMapperProtMapper | SIGNOR:30520731ProtMapper:30520731 |
| PAK1 | Q13153 | STIM1 | Q13586 | Yes | Yes | No | SIGNOR | SIGNOR:29780159 |
| AAKB1 | Q9Y478 | STIM1 | Q13586 | Yes | No | Yes | PhosphoSite_norefSIGNORPhosphoSite_ProtMapperProtMapper | SIGNOR:31381180 |
| CDK1 | P06493 | STIM1 | Q13586 | Yes | No | Yes | Sparser_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetSIGNORProtMapperSIGNOR_ProtMapperREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper | ProtMapper:19881501PhosphoSite:22748319ProtMapper:22298426SIGNOR:19881501 |
| AAPK1 | Q13131 | STIM1 | Q13586 | Yes | No | Yes | SIGNORPhosphoSitePhosphoSite_ProtMapperProtMapper | SIGNOR:31381180PhosphoSite:31381180 |
| MK01 | P28482 | STIM1 | Q13586 | Yes | Yes | No | phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetSIGNORProtMapperSIGNOR_ProtMapperREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper | PhosphoSite:19881501ProtMapper:22298426PhosphoSite:25447552PhosphoSite:33023909PhosphoSite:28935720PhosphoSite:22298426PhosphoSite:24114840SIGNOR:22298426 |
| AAKG2 | Q9UGJ0 | STIM1 | Q13586 | Yes | No | Yes | SIGNORPhosphoSite_ProtMapperProtMapper | SIGNOR:31381180 |
| FAK2 | Q14289 | STIM1 | Q13586 | Yes | No | No | Sparser_ProtMapperProtMapperRLIMS-P_ProtMapperREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper | PhosphoSite:30975919PhosphoSite:28218251ProtMapper:28218251 |
| KAPCA | P17612 | STIM1 | Q13586 | Yes | No | No | PhosphoSitePhosphoSite_ProtMapperProtMapper | PhosphoSite:29326165PhosphoSite:25504574 |
Protein Complex Composition
6 records.
| Component Name | Component Gene Symbols | Component UniProt ID | Stoichiometry | Database | Database IDs | References |
|---|---|---|---|---|---|---|
| STIM1-TRPV4 complex | STIM1TRPV4 | Q13586Q9HBA0 | 0:0 | CORUM | CORUM:6108 | 25972993 |
| OXCT1PPT1S100A16SEC22BSEPTIN11STIM1TCOF1TMED10TMEM9TSTXRCC1 | O75396P18887P49755P50897P55809Q13428Q13586Q16762Q96FQ6Q9NVA2Q9P0T7 | 0:0:0:0:0:0:0:0:0:0:0 | Havugimana2012 | Havugimana2012:C_597 | ||
| FERMT2MAD2L1BPS100A16SEC22BSEPTIN11SEPTIN9STIM1SUCLG1SUCLG2 | O75396P53597Q13586Q15013Q96AC1Q96FQ6Q96I99Q9NVA2Q9UHD8 | 0:0:0:0:0:0:0:0:0 | Havugimana2012 | Havugimana2012:C_574 | ||
| STIM1 | Q13586 | 2 | PDB | PDB:3teqPDB:2majPDB:4o9b | ||
| BRINP3STIM1STIM2 | Q13586Q76B58Q9P246 | 0:0:0 | hu.MAP2 | |||
| STIM1STIM2 | Q13586Q9P246 | 0:0 | hu.MAP2 |
Isolation & Detection Technology
1 record.
| EV Isolation Method | Detection Method | Number of References | References |
|---|---|---|---|
| Differential UltracentrifugationUltrafiltration / Tangential Flow FiltrationSize Exclusion ChromatographyImmunoaffinity Capture | Mass spectrometryWestern blottingFlow cytometry | 9 | 315085003407177739436214386443523279541426884841400983463076053840091455 |
Sequence, Structure & Domains
Sequences
Length
685
Mass
77,423
Sequence
MDVCVRLALWLLWGLLLHQGQSLSHSHSEKATGTSSGANSEESTAAEFCRIDKPLCHSEDEKLSFEAVRNIHKLMDDDANGDVDVEESDEFLREDLNYHDPTVKHSTFHGEDKLISVEDLWKAWKSSEVYNWTVDEVVQWLITYVELPQYEETFRKLQLSGHAMPRLAVTNTTMTGTVLKMTDRSHRQKLQLKALDTVLFGPPLLTRHNHLKDFMLVVSIVIGVGGCWFAYIQNRYSKEHMKKMMKDLEGLHRAEQSLHDLQERLHKAQEEHRTVEVEKVHLEKKLRDEINLAKQEAQRLKELREGTENERSRQKYAEEELEQVREALRKAEKELESHSSWYAPEALQKWLQLTHEVEVQYYNIKKQNAEKQLLVAKEGAEKIKKKRNTLFGTFHVAHSSSLDDVDHKILTAKQALSEVTAALRERLHRWQQIEILCGFQIVNNPGIHSLVAALNIDPSWMGSTRPNPAHFIMTDDVDDMDEEIVSPLSMQSPSLQSSVRQRLTEPQHGLGSQRDLTHSDSESSLHMSDRQRVAPKPPQMSRAADEALNAMTSNGSHRLIEGVHPGSLVEKLPDSPALAKKALLALNHGLDKAHSLMELSPSAPPGGSPHLDSSRSHSPSSPDPDTPSPVGDSRALQASRNTRIPHLAGKKAVAEEDNGSIGEETDSSPGRKKFPLKIFKKPLKK
Alternative Products
Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q13586-1; Sequence=Displayed; Name=2; IsoId=Q13586-2; Sequence=VSP_055150, VSP_055151
Alternative Sequence
515..540; DLTHSDSESSLHMSDRQRVAPKPPQM -> GSSLKANRLSSKGFDPFRFGVLPPHE (in isoform 2); 541..685; Missing (in isoform 2)
3D Structural Models
Turn
85..90; 272..275
Helix
64..73; 91..95; 102..108; 117..126; 128..131; 134..145; 150..157; 163..167; 171..174; 183..199; 249..271; 276..334; 339..341; 345..391; 393..397; 400..404; 408..437
Beta Strand
80..83; 110..112
3D Structure
NMR spectroscopy (4); X-ray crystallography (2)
Domain & Motif Annotations
Compositional Bias
490..499; Low complexity; 515..532; Basic and acidic residues; 608..620; Low complexity; 655..666; Acidic residues; 670..685; Basic residues
Motif
642..645; Microtubule tip localization signal
Coiled Coil
248..442
Domain (CC)
The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.; DOMAIN: The EF-hand domain is responsible for Ca(2+) sensitivity. It consists of a canonical helix-loop-helix EF motif (alpha1beta1alpha2; EF-hand 1) paired to a second helix-loop-helix EF motif (alpha3beta2alpha4; EF-hand 2). EF-hand 1 binds Ca(2+) whereas EF-hand 2 mediates the interactions with SAM domain.; DOMAIN: The sterile alpha motif (SAM) domain folds into a characteristic 5-helix bundle (alpha6-alpha10) which interacts with the EF-hand pairs enabling concerted folding and stability of EF-hand and SAM domains.; DOMAIN: The STIM1 Orai-activating region/CRAC-activating domain (SOAR/CAD) directly interacts with ORAI1 subunits and mediates CRAC channel gating.; DOMAIN: The polybasic Lys-rich region (residues 672-685) functionally interacts with the Pro-rich region of ORAI1 (residues 3-47) and regulates CRAC channel gating at negative membrane potentials.
Domain (FT)
64..97; EF-hand 1; 102..126; EF-hand 2; 132..200; SAM
Region
344..442; SOAR/CAD; 475..483; Contributes to fast Ca(2+)-dependent inactivation of CRAC channels; 490..542; Disordered; 596..685; Disordered; 672..685; Required for generation of inwardly rectifying CRAC currents
Clinical Relevance
Disease Involvement
Disease variant
Drugs
Interaction Protein
ENSG00000101367ENSG00000109689ENSG00000139514ENSG00000170027ENSG00000198833ENSG00000276045
Interaction Count
6
Interaction Dataset
intact_biogridintact_biogrid_opencellbiogrid_bioplex