Protein detail

NID2

Nidogen-2 (NID-2) (Osteonidogen)

Entry name NID2	UniProt ID Q14112	EVMP confidence score 0.50
Supporting publications (n) 2	Transmembrane count	Protein classification Plasma proteinsPredicted intracellular proteinsPredicted secreted proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information10

Protein Names

Nidogen-2 (NID-2) (Osteonidogen)

Protein Class (3)

Plasma proteinsPredicted intracellular proteinsPredicted secreted proteins

Protein Function (2)

Predicted secreted proteins
Predicted intracellular proteins

Ensembl

ENSG00000087303

Entrez Gene Symbol

NID2

Gene Description

Nidogen 2

Chromosome

Position

52004809-52069059

Supporting publications (n)

EVMP confidence score

0.50

Fluorescence & Localization5

Tissue SpecificbrainCell SpecificAdrenal medulla cellsBlood Cell SpecificbasophilBlood Lineage Specificgranulocytes

Function & Pathway8

Protein Function (2)

Predicted secreted proteins
Predicted intracellular proteins

Cellular Component (6)

Molecular Function (4)

Biological Process (3)

KEGG

hsa04820 Cytoskeleton in muscle cells

Reactome (2)

Canonical Pathways

M276 Pid fgf pathway

Mediation Categories

Adhesion and uptake mediation

Relations & Evidence36

Ligand-Receptor Signaling (32)

32 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
ecm	ecm	MatrixDB	Yes	No	Yes	No	No
ecm	ecm	GO_Intercell	Yes	No	Yes	No	No
ecm	ecm	Ramilowski_location	Yes	No	Yes	No	No
basement membrane	ecm	Ramilowski_location	Yes	No	Yes	No	No
ecm	ecm	UniProt_location	Yes	No	Yes	No	No
ecm	ecm	CellCellInteractions	Yes	No	Yes	No	No
ecm	ecm	OmniPath	Yes	No	Yes	No	No
extracellular	extracellular	LOCATE	No	No	Yes	No	No
extracellular	extracellular	OmniPath	No	No	Yes	No	No
matrix_adhesion	matrix_adhesion	Zhong2015	No	Yes	Yes	No	No

Page 1 of 4Next

Protein Complex Composition (3)

3 records.

Component Gene Symbols	Component UniProt ID	Stoichiometry	Database
B4GALNT4 LYG2 NID2 PILRA PILRB	Q14112 Q76KP1 Q86SG7 Q9UKJ0 Q9UKJ1	0:0:0:0:0	hu.MAP2
LYG2 NID2	Q14112 Q86SG7	0:0	hu.MAP2
LRRTM4 NID2 PILRA PILRB	Q14112 Q86VH4 Q9UKJ0 Q9UKJ1	0:0:0:0	hu.MAP

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion Chromatography	Mass spectrometry [LTQ-FT Ultra]R SequencingMass spectrometry	3	32384937 32301581 29603567

Sequence, Structure & Domains9

Sequences

Length

1,375

Mass

151,254

Sequence

MEGDRVAGRPVLSSLPVLLLLPLLMLRAAALHPDELFPHGESWGDQLLQEGDDESSAVVKLANPLHFYEARFSNLYVGTNGIISTQDFPRETQYVDYDFPTDFPAIAPFLADIDTSHGRGRVLYREDTSPAVLGLAARYVRAGFPRSARFTPTHAFLATWEQVGAYEEVKRGALPSGELNTFQAVLASDGSDSYALFLYPANGLQFLGTRPKESYNVQLQLPARVGFCRGEADDLKSEGPYFSLTSTEQSVKNLYQLSNLGIPGVWAFHIGSTSPLDNVRPAAVGDLSAAHSSVPLGRSFSHATALESDYNEDNLDYYDVNEEEAEYLPGEPEEALNGHSSIDVSFQSKVDTKPLEESSTLDPHTKEGTSLGEVGGPDLKGQVEPWDERETRSPAPPEVDRDSLAPSWETPPPYPENGSIQPYPDGGPVPSEMDVPPAHPEEEIVLRSYPASGHTTPLSRGTYEVGLEDNIGSNTEVFTYNAANKETCEHNHRQCSRHAFCTDYATGFCCHCQSKFYGNGKHCLPEGAPHRVNGKVSGHLHVGHTPVHFTDVDLHAYIVGNDGRAYTAISHIPQPAAQALLPLTPIGGLFGWLFALEKPGSENGFSLAGAAFTHDMEVTFYPGEETVRITQTAEGLDPENYLSIKTNIQGQVPYVSANFTAHISPYKELYHYSDSTVTSTSSRDYSLTFGAINQTWSYRIHQNITYQVCRHAPRHPSFPTTQQLNVDRVFALYNDEERVLRFAVTNQIGPVKEDSDPTPGNPCYDGSHMCDTTARCHPGTGVDYTCECASGYQGDGRNCVDENECATGFHRCGPNSVCINLPGSYRCECRSGYEFADDRHTCILITPPANPCEDGSHTCAPAGQARCVHHGGSTFSCACLPGYAGDGHQCTDVDECSENRCHPAATCYNTPGSFSCRCQPGYYGDGFQCIPDSTSSLTPCEQQQRHAQAQYAYPGARFHIPQCDEQGNFLPLQCHGSTGFCWCVDPDGHEVPGTQTPPGSTPPHCGPSPEPTQRPPTICERWRENLLEHYGGTPRDDQYVPQCDDLGHFIPLQCHGKSDFCWCVDKDGREVQGTRSQPGTTPACIPTVAPPMVRPTPRPDVTPPSVGTFLLYTQGQQIGYLPLNGTRLQKDAAKTLLSLHGSIIVGIDYDCRERMVYWTDVAGRTISRAGLELGAEPETIVNSGLISPEGLAIDHIRRTMYWTDSVLDKIESALLDGSERKVLFYTDLVNPRAIAVDPIRGNLYWTDWNREAPKIETSSLDGENRRILINTDIGLPNGLTFDPFSKLLCWADAGTKKLECTLPDGTGRRVIQNNLKYPFSIVSYADHFYHTDWRRDGVVSVNKHSGQFTDEYLPEQRSHLYGITAVYPYCPTGRK

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q14112-1; Sequence=Displayed; Name=2; IsoId=Q14112-2; Sequence=VSP_038779, VSP_038780

Alternative Sequence

7..59; Missing (in isoform 2); 844..892; LITPPANPCEDGSHTCAPAGQARCVHHGGSTFSCACLPGYAGDGHQCTD -> Y (in isoform 2)

Domain & Motif Annotations

Compositional Bias

386..403; Basic and acidic residues; 999..1014; Pro residues

Repeat

1154..1197; LDL-receptor class B 1; 1198..1240; LDL-receptor class B 2; 1241..1285; LDL-receptor class B 3; 1286..1327; LDL-receptor class B 4; 1329..1373; LDL-receptor class B 5

Domain (FT)

108..273; NIDO; 484..524; EGF-like 1; 528..758; Nidogen G2 beta-barrel; 759..800; EGF-like 2; 801..843; EGF-like 3; calcium-binding; 848..891; EGF-like 4; 892..930; EGF-like 5; calcium-binding; 937..1005; Thyroglobulin type-1 1; 1016..1084; Thyroglobulin type-1 2

Region

348..437; Disordered; 992..1016; Disordered

Clinical Relevance4

Antibody (2)

HPA052460 HPA058772

Interaction Protein

ENSG00000135862

Interaction Count

Interaction Dataset

biogrid_bioplex

Supporting Publications2

PMID	Title	Abstract
35119778	Optimization of small extracellular vesicle isolation from expressed prostatic secretions in urine for in-depth proteomic analysis.	No abstract available
38716512	Assessment of urine sample collection and processing variables for extracellular vesicle-based proteomics.	No abstract available