Protein detail

GRB7

Growth factor receptor-bound protein 7 (B47) (Epidermal growth factor receptor GRB-7) (GRB7 adapter protein)

Entry name GRB7	UniProt ID Q14451	EVMP confidence score 0.72
Supporting publications (n) 15	Transmembrane count	Protein classification Cancer-related genesPredicted intracellular proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information10

Protein Names

Growth factor receptor-bound protein 7 (B47) (Epidermal growth factor receptor GRB-7) (GRB7 adapter protein)

Protein Class (2)

Cancer-related genesPredicted intracellular proteins

Protein Function (2)

Cancer-related genes:Candidate cancer biomarkers
Predicted intracellular proteins

Ensembl

ENSG00000141738

Entrez Gene Symbol

GRB7

Gene Description

Growth factor receptor bound protein 7

Chromosome

Position

39737927-39747291

Supporting publications (n)

EVMP confidence score

0.72

Fluorescence & Localization1

Function & Pathway6

Protein Function (2)

Cancer-related genes:Candidate cancer biomarkers
Predicted intracellular proteins

Cellular Component (5)

Molecular Function (5)

Biological Process (3)

Reactome (14)

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Mediation Categories (3)

Adhesion and uptake mediationImmune mediationReceptor-signaling mediation

Relations & Evidence29

Enzyme-Mediated Modification (5)

5 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
GRB7	PTK2	Q05397	Y	188	phosphorylation	dbPTMPhosphoSitePhosphoSite_ProtMapperProtMapper	dbPTM:19473962
GRB7	PTK2	Q05397	Y	338	phosphorylation	dbPTMPhosphoSitePhosphoSite_ProtMapperProtMapper	dbPTM:19473962
GRB7	PTK2	Q05397	Y	211	phosphorylation	MIMPPhosphoSite_MIMP
GRB7	PTK2	Q05397	Y	361	phosphorylation	MIMPPhosphoSite_MIMP
GRB7	MAPK8	P45983	S	194	phosphorylation	PhosphoSitePhosphoSite_ProtMapperProtMapper

Ligand-Receptor Signaling (12)

12 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
cell_adhesion	cell_adhesion	Cellinker	Yes	Yes	No	No	No
cell_adhesion	cell_adhesion	OmniPath	Yes	Yes	No	No	No
adhesion_cytoskeleton_adaptor	intracellular_intercellular_related	Adhesome	Yes	No	No	No	No
intracellular_intercellular_related	intracellular_intercellular_related	OmniPath	Yes	No	No	No	No
plasma_membrane	plasma_membrane	UniProt_location	No	No	No	No	No

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Regulatory Interaction Network (9)

9 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
KIT	P10721	GRB7	Q14451	Yes	Yes	No	WangNetPathPhosphoPointSIGNORHPRDCui2007ELMHINTCancerCellMapSPIKE_LCLit-BM-17SPIKE	HINT:10377264 SPIKE:10377264 CancerCellMap:10377264 Lit-BM-17:24728074 Lit-BM-17:10377264 SIGNOR:10377264 HPRD:10377264 SPIKE_LC:10377264 NetPath:10377264 ELM:10377264
EPHB1	P54762	GRB7	Q14451	Yes	Yes	No	Li2012Lit-BM-17SignaLink3	Lit-BM-17:12223469 SignaLink3:23331499 SignaLink3:12223469
TIE2	Q02763	GRB7	Q14451	Yes	Yes	No	PhosphoPointHPRDSignaLink3Li2012Wang	HPRD:10521483 SignaLink3:10521483 SignaLink3:23331499
INSR	P06213	GRB7	Q14451	Yes	Yes	No	AdhesomePhosphoPointSIGNORHPRDSignaLink3Li2012WangLit-BM-17	Lit-BM-17:10803466 SignaLink3:10803466 Adhesome:10803466 SIGNOR:10803466 SignaLink3:23331499 HPRD:10803466
RET	P07949	GRB7	Q14451	Yes	Yes	No	PhosphoPointSIGNORHPRDSignaLink3Li2012SPIKE_LCLit-BM-17	SignaLink3:8631863 HPRD:8631863 SignaLink3:23331499 Lit-BM-17:8631863 SIGNOR:8631863 SPIKE_LC:16189514
GRB7	Q14451	RND1	Q92730	Yes	Yes	No	HPRDLit-BM-17SIGNORSignaLink3	SIGNOR:10664463 Lit-BM-17:10664463 HPRD:10664463 SignaLink3:10664463 SignaLink3:23331499
MK08	P45983	GRB7	Q14451	Yes	No	Yes	SIGNORPhosphoSitePhosphoSite_ProtMapperProtMapper	SIGNOR:27658202 PhosphoSite:27658202
PTN11	Q06124	GRB7	Q14451	Yes	Yes	No	Li2012SIGNORAdhesome	Adhesome:8622870 SIGNOR:20142421
FAK1	Q05397	GRB7	Q14451	Yes	Yes	No	WangAdhesomeMIMPPhosphoSite_MIMPPhosphoSite_norefPhosphoPointiPTMnetProtMapperHPRDCui2007HINTdbPTMLi2012SPIKE_LCLit-BM-17PhosphoSitePhosphoSite_ProtMapper	Lit-BM-17:25814554 Lit-BM-17:10446223 Adhesome:10446223 PhosphoSite:19473962 Adhesome:12021278 HINT:10446223 dbPTM:19473962 HPRD:10446223 Adhesome:17318180 HINT:11119718 SPIKE_LC:16189514

Protein Complex Composition (2)

2 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
GRB7-PTK2 complex	GRB7 PTK2	Q05397 Q14451	0:0	CORUM	CORUM:7116	10446223
	GRB7	Q14451	4	PDB	PDB:5d0jPDB:5u06PDB:4x6sPDB:3pqzPDB:4wwqPDB:5eeqPDB:2qmsPDB:5u1qPDB:5tyiPDB:5eelPDB:7mp3

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationDensity Gradient CentrifugationUltrafiltration / Tangential Flow FiltrationSize Exclusion Chromatography	Mass spectrometry	6	40563475 35611462 34817906 28986585 27894104 38321535

Sequence, Structure & Domains15

Sequences

Length

532

Mass

59,681

Sequence

MELDLSPPHLSSSPEDLCPAPGTPPGTPRPPDTPLPEEVKRSQPLLIPTTGRKLREEERRATSLPSIPNPFPELCSPPSQSPILGGPSSARGLLPRDASRPHVVKVYSEDGACRSVEVAAGATARHVCEMLVQRAHALSDETWGLVECHPHLALERGLEDHESVVEVQAAWPVGGDSRFVFRKNFAKYELFKSSPHSLFPEKMVSSCLDAHTGISHEDLIQNFLNAGSFPEIQGFLQLRGSGRKLWKRFFCFLRRSGLYYSTKGTSKDPRHLQYVADVNESNVYVVTQGRKLYGMPTDFGFCVKPNKLRNGHKGLRIFCSEDEQSRTCWLAAFRLFKYGVQLYKNYQQAQSRHLHPSCLGSPPLRSASDNTLVAMDFSGHAGRVIENPREALSVALEEAQAWRKKTNHRLSLPMPASGTSLSAAIHRTQLWFHGRISREESQRLIGQQGLVDGLFLVRESQRNPQGFVLSLCHLQKVKHYLILPSEEEGRLYFSMDDGQTRFTDLLQLVEFHQLNRGILPCLLRHCCTRVAL

Alternative Products

Event=Alternative splicing; Named isoforms=4; Comment=At least 2 isoforms are produced.; Name=1; IsoId=Q14451-1; Sequence=Displayed; Name=2; Synonyms=Grb7V; IsoId=Q14451-2; Sequence=VSP_035500, VSP_035501; Name=3; IsoId=Q14451-3; Sequence=VSP_041665; Name=4; IsoId=Q14451-4; Sequence=VSP_041666

Alternative Sequence

1; M -> MGKWRPGQGHTTGSVKPLSCSDAM (in isoform 3); 48..81; Missing (in isoform 4); 425..447; IHRTQLWFHGRISREESQRLIGQ -> CSWSGRVSGTPRALSSLCATCRK (in isoform 2); 448..532; Missing (in isoform 2)

3D Structural Models

Turn

150..152

Helix

124..132; 164..168; 421..424; 425..428; 429..431; 438..447; 505..512

Beta Strand

102..108; 113..118; 134..136; 145..148; 155..158; 160..162; 173..175; 178..181; 432..436; 455..459; 461..463; 467..473; 476..487; 490..496; 502..504; 513..515; 519..521

3D Structure

NMR spectroscopy (3); X-ray crystallography (11)

Domain & Motif Annotations

Compositional Bias

1..20; Low complexity; 21..34; Pro residues

Domain (CC)

The PH domain mediates interaction with membranes containing phosphoinositides.

Domain (FT)

100..186; Ras-associating; 229..338; PH; 431..527; SH2

Region

1..96; Disordered

Protein Families

GRB7/10/14 family

Sequence Similarities

Belongs to the GRB7/10/14 family.

Clinical Relevance6

Disease Involvement

Cancer-related genes

Drugs (2)

GSK180 GSK065

Antibody (3)

CAB005226 HPA057084 CAB073538

Interaction Protein (8)

ENSG00000065361ENSG00000141736ENSG00000154928ENSG00000157404ENSG00000169398ENSG00000172602ENSG00000184221ENSG00000204424

Interaction Count

Interaction Dataset

intact_biogrid

Supporting Publications15

PMID	Title	Abstract
26826536	[Cardiovascular risk study in patients with renin-angiotensin system blockade by means of the proteone of circulating extracellular vesicles].	No abstract available
32560723	T2 and T17 cytokines alter the cargo and function of airway epithelium-derived extracellular vesicles.	No abstract available
32854315	Proteomic Profiling of Extracellular Vesicles Derived from Cerebrospinal Fluid of Alzheimer's Disease Patients: A Pilot Study.	Recent studies have highlighted the importance of Aβ and tau-containing extracellular vesicles (EVs) in AD.
33592500	A Proteomic Approach to Understand the Clinical Significance of Acute Myeloid Leukemia-Derived Extracellular Vesicles Reflecting Essential Characteristics of Leukemia.	No abstract available
34064677	Ubiquinone Metabolism and Transcription HIF-1 Targets Pathway Are Toxicity Signature Pathways Present in Extracellular Vesicles of Paraquat-Exposed Human Brain Microvascular Endothelial Cells.	No abstract available
36064647	Systemic proteomics and miRNA profile analysis of exosomes derived from human pluripotent stem cells.	No abstract available
36146834	Human Cytomegalovirus Modifies Placental Small Extracellular Vesicle Composition to Enhance Infection of Fetal Neural Cells In Vitro.	No abstract available
37322475	Comprehensive profiling of extracellular vesicles in uveitis and scleritis enables biomarker discovery and mechanism exploration.	No abstract available
37926756	Extracellular vesicles from non-neuroendocrine SCLC cells promote adhesion and survival of neuroendocrine SCLC cells.	No abstract available
38113368	In-Depth Proteome Profiling of Small Extracellular Vesicles Isolated from Cancer Cell Lines and Patient Serum.	No abstract available

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