Protein detail

ARHG6

Rho guanine nucleotide exchange factor 6 (Alpha-Pix) (COOL-2) (PAK-interacting exchange factor alpha) (Rac/Cdc42 guanine nucleotide exchange factor 6)

Entry name ARHG6	UniProt ID Q15052	EVMP confidence score 0.38
Supporting publications (n) 5	Transmembrane count	Protein classification Cancer-related genesPredicted intracellular proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

Rho guanine nucleotide exchange factor 6 (Alpha-Pix) (COOL-2) (PAK-interacting exchange factor alpha) (Rac/Cdc42 guanine nucleotide exchange factor 6)

Protein Class (2)

Cancer-related genesPredicted intracellular proteins

Protein Function (2)

Cancer-related genes:Mutational cancer driver genes
Predicted intracellular proteins

Ensembl

ENSG00000129675

Entrez Gene Symbol

ARHGEF6

Gene Synonym (6)

alpha-PIXalphaPIXCool-2Cool2KIAA0006MRX46

Gene Description

Rac/Cdc42 guanine nucleotide exchange factor 6

Chromosome

Position

136665547-136780932

Supporting publications (n)

EVMP confidence score

0.38

Function & Pathway7

Protein Function (2)

Cancer-related genes:Mutational cancer driver genes
Predicted intracellular proteins

Cellular Component (4)

Molecular Function (3)

Biological Process (3)

KEGG (3)

Reactome (17)

Page 1 of 2

Mediation Categories

Receptor-signaling mediation

Relations & Evidence18

Enzyme-Mediated Modification (6)

6 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
ARHGEF6	PRKCQ	Q04759	S	225	phosphorylation	PhosphoSitePhosphoSite_ProtMapperProtMapper
ARHGEF6	PRKCQ	Q04759	S	488	phosphorylation	PhosphoSitePhosphoSite_ProtMapperProtMapper
ARHGEF6	PRKACA	P17612	S	684	phosphorylation	PhosphoSitePhosphoSite_ProtMapperProtMapper
ARHGEF6	PRKG1	Q13976	S	684	phosphorylation	PhosphoSitePhosphoSite_ProtMapperProtMapper
ARHGEF6	GSK3B	P49841	S	225	phosphorylation	KEA	KEA:17570479
ARHGEF6	MAPK9	P45984	S	225	phosphorylation	KEA	KEA:17570479

Ligand-Receptor Signaling (4)

4 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No

Regulatory Interaction Network (5)

5 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
KAPCA	P17612	ARHG6	Q15052	Yes	No	Yes	iPTMnetPhosphoSite_norefSIGNORProtMapperPhosphoSite_ProtMapper	SIGNOR:26507661
ASAP3	Q8TDY4	ARHG6	Q15052	Yes	Yes	No	SIGNOR	SIGNOR:23776207
KPCT	Q04759	ARHG6	Q15052	Yes	Yes	No	PhosphoSite_norefSIGNORiPTMnetProtMapperPhosphoSitePhosphoSite_ProtMapper	PhosphoSite:25694429 SIGNOR:25694429
GIT1	Q9Y2X7	ARHG6	Q15052	Yes	Yes	No	AdhesomeSIGNORHPRDHINTWangLit-BM-17	Adhesome:10428811 Adhesome:21900206 Lit-BM-17:21900206 Adhesome:10592173 HPRD:10896954 HINT:10896954 Adhesome:16373173 SIGNOR:26507661 Adhesome:10896954 Lit-BM-17:10428811 HINT:33961781
ARHG6	Q15052	RAC1	P63000	Yes	Yes	No	WangSIGNORAdhesome	Adhesome:16492808 SIGNOR:26507661 Adhesome:10592173

Protein Complex Composition (2)

2 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
HT_DM_Cluster449	ARHGEF6 ARHGEF7 GIT1 GIT2	Q14155 Q14161 Q15052 Q9Y2X7	1:1:1:1	Compleathu.MAP2	Compleat:HC2946	22036573
	ARHGEF6 DOK1 NCK2 WIPF3	A6NGB9 O43639 Q15052 Q99704	0:0:0:0	hu.MAP

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationDensity Gradient CentrifugationUltrafiltration / Tangential Flow FiltrationSize Exclusion ChromatographyPolymer PrecipitationImmunoaffinity Capture	Mass spectrometryMass spectrometry [LTQ-FT Ultra]Mass spectrometry [QTOF]Small R sequencing (Illumi HiSeq 2000 (Solexa)	52	31196968 32284825 32341357 33204424 35611462 36797805 32489530 32759820 32944193 34623918 36612172 39766158 39940641 29436780 30379855 36398564 37786918 38168906 36217952 40748658 39940923 39505756 39409015 37205098 34817906 33169421 27234505 28986585 40563475 21630462 22740476 27894104 28590090 29045505 29687628 29740045 30646616 30950185 31081149 31941606 32295833 34571828 38321535 39558134 39569406 34445582 20031610 29148239 30408591 32089743 29148239 39569406

Sequence, Structure & Domains12

Sequences

Length

776

Mass

87,499

Sequence

MNPEEQIVTWLISLGVLESPKKTICDPEEFLKSSLKNGVVLCKLINRLMPGSVEKFCLDPQTEADCINNINDFLKGCATLQVEIFDPDDLYSGVNFSKVLSTLLAVNKATEDQLSERPCGRSSSLSAANTSQTNPQGAVSSTVSGLQRQSKTVEMTENGSHQLIVKARFNFKQTNEDELSVCKGDIIYVTRVEEGGWWEGTLNGRTGWFPSNYVREIKSSERPLSPKAVKGFETAPLTKNYYTVVLQNILDTEKEYAKELQSLLVTYLRPLQSNNNLSTVEVTSLLGNFEEVCTFQQTLCQALEECSKFPENQHKVGGCLLSLMPHFKSMYLAYCANHPSAVNVLTQHSDELEQFMENQGASSPGILILTTNLSKPFMRLEKYVTLLQELERHMEDTHPDHQDILKAIVAFKTLMGQCQDLRKRKQLELQILSEPIQAWEGEDIKNLGNVIFMSQVMVQYGACEEKEERYLMLFSNVLIMLSASPRMSGFIYQGKIPIAGTVVTRLDEIEGNDCTFEITGNTVERIVVHCNNNQDFQEWLEQLNRLIRGPASCSSLSKTSSSSCSAHSSFSSTGQPRGPLEPPQIIKPWSLSCLRPAPPLRPSAALGYKERMSYILKESSKSPKTMKKFLHKRKTERKPSEEEYVIRKSTAALEEDAQILKVIEAYCTSANFQQGHGSSTRKDSIPQVLLPEEEKLIIEETRSNGQTIMEEKSLVDTVYALKDEVRELKQENKRMKQCLEEELKSRRDLEKLVRRLLKQTDECIRGESSSKTSILP

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q15052-1; Sequence=Displayed; Name=2; IsoId=Q15052-2; Sequence=VSP_015782

Alternative Sequence

1..154; Missing (in isoform 2)

3D Structural Models

Turn

211..213

Helix

4..14; 27..37; 39..48; 63..80; 87..91; 96..110; 219..222

Beta Strand

163..166; 187..191; 194..196; 198..202; 205..209; 214..216

3D Structure

NMR spectroscopy (2)

Domain & Motif Annotations

Compositional Bias

121..151; Polar residues; 561..572; Low complexity

Domain (FT)

1..111; Calponin-homology (CH); 160..219; SH3; 241..421; DH; 443..548; PH

Region

115..151; Disordered; 561..581; Disordered

Clinical Relevance5

Disease Involvement

Cancer-related genes

Antibody (2)

HPA003578 HPA071036

Interaction Protein (5)

ENSG00000065054ENSG00000102606ENSG00000108262ENSG00000149269ENSG00000180370

Interaction Count

Interaction Dataset (3)

intact_biogridbiogrid_opencellintact_biogrid_opencell

Supporting Publications5

PMID	Title	Abstract
32854315	Proteomic Profiling of Extracellular Vesicles Derived from Cerebrospinal Fluid of Alzheimer's Disease Patients: A Pilot Study.	Recent studies have highlighted the importance of Aβ and tau-containing extracellular vesicles (EVs) in AD.
37322475	Comprehensive profiling of extracellular vesicles in uveitis and scleritis enables biomarker discovery and mechanism exploration.	No abstract available
38207106	Proteomic, Metabolomic, and Fatty Acid Profiling of Small Extracellular Vesicles from Glioblastoma Stem-Like Cells and Their Role in Tumor Heterogeneity.	No abstract available
40985879	TurboID-Mediated Profiling of Glioblastoma-Derived Extracellular Vesicle Cargo Proteins.	No abstract available
41307968	Extracellular Vesicles Define Discrete Nano-Based Niches Within the Human Haematopoietic System.	No abstract available