Protein detail

RAB35

Ras-related protein Rab-35 (EC 3.6.5.2) (GTP-binding protein RAY) (Ras-related protein Rab-1C)

Entry name RAB35	UniProt ID Q15286	EVMP confidence score 0.50
Supporting publications (n)	Transmembrane count	Protein classification Predicted intracellular proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information10

Protein Names

Ras-related protein Rab-35 (EC 3.6.5.2) (GTP-binding protein RAY) (Ras-related protein Rab-1C)

Protein Class

Predicted intracellular proteins

Protein Function

Predicted intracellular proteins

Ensembl

ENSG00000111737

Entrez Gene Symbol

RAB35

Gene Synonym

H-ray

Gene Description

RAB35, member RAS oncogene family

Chromosome

Position

120095099-120117502

EVMP confidence score

0.50

Fluorescence & Localization4

Tissue Specificblood vesselCell SpecificAlveolar cells type 1Blood Cell Specificnaive B-cellBlood Lineage SpecificB-cells

Function & Pathway7

Protein Function

Predicted intracellular proteins

Cellular Component (11)

Page 1 of 2

Molecular Function (5)

Biological Process (3)

KEGG

hsa04144 Endocytosis

Reactome (7)

Mediation Categories (4)

Fusion and delivery mediationImmune mediationMetabolism mediationReceptor-signaling mediation

Relations & Evidence15

Ligand-Receptor Signaling (6)

6 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
plasma_membrane	plasma_membrane	UniProt_location	No	No	No	No	No
plasma_membrane	plasma_membrane	OmniPath	No	No	No	No	No

Protein Complex Composition (8)

8 records.

Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs
ALDH7A1 COPB2 IDH3A IDH3B IDH3G RAB35 UBC	O43837 P0CG48 P35606 P49419 P50213 P51553 Q15286	1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC7331
COPB2 IDH3A IDH3B IDH3G RAB35 RBX1 UBC	O43837 P0CG48 P35606 P50213 P51553 P62877 Q15286	1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC6285
IDH3A IDH3B IDH3G RAB35 THOC6 UBC	O43837 P0CG48 P50213 P51553 Q15286 Q86W42	1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC4607
FTSJ1 IDH3A IDH3B IDH3G RAB35 UBC	O43837 P0CG48 P50213 P51553 Q15286 Q9UET6	1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC5795
GDI1 RAB12 RAB35 RAB4A	P20338 P31150 Q15286 Q6IQ22	0:0:0:0	hu.MAP
DENND1B RAB35	Q15286 Q6P3S1	2:2	PDB	PDB:3tw8
RAB35 RUSC2	Q15286 Q8N2Y8	1:1	PDB	PDB:6if2
DENND1A RAB35	Q15286 Q8TEH3	2:2	PDB	PDB:6ekk

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion ChromatographyImmunoaffinity Capture	Mass spectrometryFlow cytometryELISA	4	37862381 31508500 26775013 40098346

Sequence, Structure & Domains13

Sequences

Length

201

Mass

23,025

Sequence

MARDYDHLFKLLIIGDSGVGKSSLLLRFADNTFSGSYITTIGVDFKIRTVEINGEKVKLQIWDTAGQERFRTITSTYYRGTHGVIVVYDVTSAESFVNVKRWLHEINQNCDDVCRILVGNKNDDPERKVVETEDAYKFAGQMGIQLFETSAKENVNVEEMFNCITELVLRAKKDNLAKQQQQQQNDVVKLTKNSKRKKRCC

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q15286-1; Sequence=Displayed; Name=2; IsoId=Q15286-2; Sequence=VSP_042918

Alternative Sequence

118..201; VGNKNDDPERKVVETEDAYKFAGQMGIQLFETSAKENVNVEEMFNCITELVLRAKKDNLAKQQQQQQNDVVKLTKNSKRKKRCC -> DVQLHHGAGPPSKERQPGKTAAATTERCGEAHEEQ (in isoform 2)

3D Structural Models

Turn

151..154

Helix

21..30; 38..41; 68..70; 74..78; 93..97; 99..109; 125..127; 132..142; 157..173

Beta Strand

4..15; 42..52; 55..64; 82..89; 114..120; 145..148

3D Structure

X-ray crystallography (4)

Domain & Motif Annotations

Motif

30..42; Switch 1; 64..80; Switch 2

Domain (CC)

Switch 1, switch 2 and the interswitch regions are characteristic of Rab GTPases and mediate the interactions with Rab downstream effectors. The switch regions undergo conformational changes upon nucleotide binding which drives interaction with specific sets of effector proteins, with most effectors only binding to GTP-bound Rab.

Protein Families (2)

Small GTPase superfamily
Rab family

Sequence Similarities

Belongs to the small GTPase superfamily. Rab family.

Clinical Relevance4

Antibody

HPA054146

Interaction Protein (5)

ENSG00000057608ENSG00000076706ENSG00000117394ENSG00000203668ENSG00000203879

Interaction Count

Interaction Dataset (3)

biogrid_opencellbiogrid_bioplexbiogrid_opencell_bioplex