Protein detail
RBP1
RalA-binding protein 1 (RalBP1) (76 kDa Ral-interacting protein) (Dinitrophenyl S-glutathione ATPase) (DNP-SG ATPase) (EC 7.6.2.2, EC 7.6.2.3) (Ral-interacting protein 1)
Entry name RBP1 | UniProt ID | EVMP confidence score 0.50 |
Supporting publications (n) 5 | Transmembrane count | Protein classification EnzymesMetabolic proteinsPredicted intracellular proteinsRAS pathway related proteinsTransporters |
EVMP confidence score
Annotation confidence score; open for threshold definitions.
Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40Basic Information11
Protein Names
RalA-binding protein 1 (RalBP1) (76 kDa Ral-interacting protein) (Dinitrophenyl S-glutathione ATPase) (DNP-SG ATPase) (EC 7.6.2.2, EC 7.6.2.3) (Ral-interacting protein 1)
Protein Class (5)
EnzymesMetabolic proteinsPredicted intracellular proteinsRAS pathway related proteinsTransporters
Protein Function (5)
- Transporters
- Predicted intracellular proteins
- RAS pathway related proteins
- Enzymes
- ENZYME proteins
Ensembl
Entrez Gene Symbol
Gene Synonym (3)
RIPRIP1RLIP76
Gene Description
RalA binding protein 1
Chromosome
18
Position
9475009-9538114
Supporting publications (n)
5
EVMP confidence score
0.50
Fluorescence & Localization3
Tissue SpecificintestineCell SpecificFoveolar cells
Function & Pathway8
Protein Function (5)
- Transporters
- Predicted intracellular proteins
- RAS pathway related proteins
- Enzymes
- ENZYME proteins
Cellular Component (7)
Molecular Function (9)
- GO:0005096 GTPase activator activity
- GO:0005515 protein binding
- GO:0005524 ATP binding
- GO:0008559 ABC-type xenobiotic transporter activity
- GO:0015431 ABC-type glutathione S-conjugate transporter activity
- GO:0022857 transmembrane transporter activity
- GO:0031267 small GTPase binding
- GO:0042626 ATPase-coupled transmembrane transporter activity
- GO:0042910 xenobiotic transmembrane transporter activity
Biological Process (3)
KEGG (3)
Reactome (4)
Canonical Pathways
M68 Pid rhoa reg pathway
Mediation Categories (4)
Adhesion and uptake mediationClinical-translation mediationFusion and delivery mediationReceptor-signaling mediation
Relations & Evidence22
Enzyme-Mediated Modification (4)
4 records.
| Substrate Gene Symbol | Enzyme Gene Symbol | Enzyme UniProt ID | Residue Type | Residue Offset | Modification | Database | References |
|---|---|---|---|---|---|---|---|
| RALBP1 | PRKCA | P17252 | T | 297 | phosphorylation | PhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperKEAPhosphoSitePhosphoSite_ProtMapper | SIGNOR:16087181KEA:16087181 |
| RALBP1 | PRKCA | P17252 | S | 118 | phosphorylation | Sparser_ProtMapperPhosphoSite_MIMPMIMPProtMapperPhosphoSitePhosphoSite_ProtMapper | ProtMapper:21411502 |
| RALBP1 | PRKCA | P17252 | S | 353 | phosphorylation | PhosphoSite_MIMPMIMPProtMapperPhosphoSitePhosphoSite_ProtMapper | |
| RALBP1 | PRKCA | P17252 | S | 509 | phosphorylation | PhosphoSite_MIMPMIMPProtMapperPhosphoSitePhosphoSite_ProtMapper |
Ligand-Receptor Signaling (10)
10 records.
| Category | Parent | Database | Transmitter | Receiver | Secreted | Plasma Membrane (Transmembrane) | Plasma Membrane (Peripheral) |
|---|---|---|---|---|---|---|---|
| intracellular | intracellular | LOCATE | No | No | No | No | No |
| intracellular | intracellular | ComPPI | No | No | No | No | No |
| intracellular | intracellular | GO_Intercell | No | No | No | No | No |
| intracellular | intracellular | UniProt_location | No | No | No | No | No |
| intracellular | intracellular | OmniPath | No | No | No | No | No |
| abc | transporter | DGIdb | No | Yes | No | No | No |
| transporter | transporter | DGIdb | No | Yes | No | No | No |
| transporter | transporter | OmniPath | No | Yes | No | No | No |
| plasma_membrane | plasma_membrane | UniProt_location | No | No | No | No | No |
| plasma_membrane | plasma_membrane | OmniPath | No | No | No | No | No |
Regulatory Interaction Network (2)
2 records.
| Source Protein Symbol | Source UniProt ID | Target Protein Symbol | Target UniProt ID | Is Directed | Is Stimulation | Is Inhibition | Database | References |
|---|---|---|---|---|---|---|---|---|
| RBP1 | Q15311 | RAC1 | P63000 | Yes | Yes | Yes | WangSIGNORHPRDCui2007HINTCA1SPIKE_LCSPIKE | HPRD:7673236HINT:7673236HINT:32203420SPIKE_LC:21686269SPIKE_LC:16189514SPIKE:21686269SIGNOR:32203420CA1:9395447 |
| KPCA | P17252 | RBP1 | Q15311 | Yes | Yes | No | Sparser_ProtMapperPhosphoSite_MIMPMIMPHPRD_MIMPPhosphoSite_norefPhosphoPointSIGNORProtMapperiPTMnetHPRDKEAHPRD_KEASIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapperSPIKE_LC | SIGNOR:16087181ProtMapper:21411502ProtMapper:16087181PhosphoSite:16087181HPRD:16087181SPIKE_LC:16087181KEA:16087181PhosphoSite:17706599 |
Protein Complex Composition (5)
5 records.
| Component Name | Component Gene Symbols | Component UniProt ID | Stoichiometry | Database | Database IDs | References |
|---|---|---|---|---|---|---|
| Caspase-2-TRAF2-RIP1 complex | CASP2RALBP1TRAF2 | P42575Q12933Q15311 | 1:1:1 | Compleat | Compleat:HC175 | 15590671 |
| RalBP1-CDC2-CCNB1 complex | CCNB1CDK1RALBP1 | P06493P14635Q15311 | 1:1:1 | CompleatCORUM | Compleat:HC1938CORUM:243 | 12775724 |
| RALBRALBP1 | P11234Q15311 | 2:2 | PDB | PDB:2kwiPDB:6zqtPDB:6zrn | ||
| MSANTD4PHLDB3RALBP1REPS2SKI | P12755Q15311Q6NSJ2Q8NCY6Q8NFH8 | 0:0:0:0:0 | hu.MAP2 | |||
| MSANTD4RALBP1REPS2 | Q15311Q8NCY6Q8NFH8 | 0:0:0 | hu.MAP2 |
Isolation & Detection Technology (1)
1 record.
| EV Isolation Method | Detection Method | Number of References | References |
|---|---|---|---|
| Differential UltracentrifugationSize Exclusion ChromatographyImmunoaffinity Capture | Mass spectrometryMass spectrometry|Western blotting|FACSFACSMass spectrometry [LTQ-FT Ultra]Western blotting|Mass spectrometryWestern blotting | 7 | 29423001358925643635976036973758375625223781268738360687 |
Sequence, Structure & Domains11
Sequences
Length
655
Mass
76,063
Sequence
MTECFLPPTSSPSEHRRVEHGSGLTRTPSSEEISPTKFPGLYRTGEPSPPHDILHEPPDVVSDDEKDHGKKKGKFKKKEKRTEGYAAFQEDSSGDEAESPSKMKRSKGIHVFKKPSFSKKKEKDFKIKEKPKEEKHKEEKHKEEKHKEKKSKDLTAADVVKQWKEKKKKKKPIQEPEVPQIDVPNLKPIFGIPLADAVERTMMYDGIRLPAVFRECIDYVEKYGMKCEGIYRVSGIKSKVDELKAAYDREESTNLEDYEPNTVASLLKQYLRDLPENLLTKELMPRFEEACGRTTETEKVQEFQRLLKELPECNYLLISWLIVHMDHVIAKELETKMNIQNISIVLSPTVQISNRVLYVFFTHVQELFGNVVLKQVMKPLRWSNMATMPTLPETQAGIKEEIRRQEFLLNCLHRDLQGGIKDLSKEERLWEVQRILTALKRKLREAKRQECETKIAQEIASLSKEDVSKEEMNENEEVINILLAQENEILTEQEELLAMEQFLRRQIASEKEEIERLRAEIAEIQSRQQHGRSETEEYSSESESESEDEEELQIILEDLQRQNEELEIKNNHLNQAIHEEREAIIELRVQLRLLQMQRAKAEQQAQEDEEPEWRGGAVQPPRDGVLEPKAAKEQPKAGKEPAKPSPSRDRKETSI
3D Structural Models
Turn
223..225; 228..232; 417..419
Helix
194..200; 211..222; 237..248; 255..257; 260..273; 280..291; 296..309; 312..334; 339..350; 354..367; 396..416; 423..443
Beta Strand
184..187; 384..386
3D Structure
NMR spectroscopy (3); X-ray crystallography (2)
Domain & Motif Annotations
Compositional Bias
24..33; Polar residues; 52..68; Basic and acidic residues; 69..79; Basic residues; 102..118; Basic residues; 119..155; Basic and acidic residues; 536..551; Acidic residues; 624..655; Basic and acidic residues
Domain (CC)
The Rho-GAP domain mediates the GTPase activator activity toward CDC42..
Domain (FT)
192..380; Rho-GAP
Region
1..158; Disordered; 102..119; Nuclear localization signal; 154..219; Mediates association with membranes and could form transmembrane domains; 403..499; Mediates interaction with RALA and RALB; 500..655; Mediates interaction with REPS1 and REPS2; 525..551; Disordered; 601..655; Disordered
Clinical Relevance5
Drugs (7)
Interaction Protein (19)
ENSG00000006451ENSG00000042753ENSG00000095066ENSG00000100211ENSG00000105619ENSG00000108091ENSG00000115977ENSG00000118898ENSG00000131149ENSG00000132522ENSG00000135597ENSG00000137504ENSG00000138756ENSG00000144118ENSG00000157540ENSG00000161203ENSG00000169891ENSG00000170903ENSG00000186193
Interaction Count
19
Interaction Dataset (5)
intact_biogridbiogrid_opencellbiogrid_bioplexintact_biogrid_bioplexintact_biogrid_opencell
Supporting Publications5
| PMID | Title | Abstract |
|---|---|---|
| 30915084 | Extracellular Vesicles Mediate Mesenchymal Stromal Cell-Dependent Regulation of B Cell PI3K-AKT Signaling Pathway and Actin Cytoskeleton. | No abstract available |
| 36146834 | Human Cytomegalovirus Modifies Placental Small Extracellular Vesicle Composition to Enhance Infection of Fetal Neural Cells In Vitro. | No abstract available |
| 38207106 | Proteomic, Metabolomic, and Fatty Acid Profiling of Small Extracellular Vesicles from Glioblastoma Stem-Like Cells and Their Role in Tumor Heterogeneity. | No abstract available |
| 40189497 | Small extracellular vesicle-based one-step high-throughput microfluidic platform for epithelial ovarian cancer diagnosis. | No abstract available |
| 40985879 | TurboID-Mediated Profiling of Glioblastoma-Derived Extracellular Vesicle Cargo Proteins. | No abstract available |