Protein detail

AAK1

AP2-associated protein kinase 1 (EC 2.7.11.1) (Adaptor-associated kinase 1)

Entry name AAK1	UniProt ID Q2M2I8	EVMP confidence score 0.50
Supporting publications (n) 3	Transmembrane count	Protein classification EnzymesPredicted intracellular proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

AP2-associated protein kinase 1 (EC 2.7.11.1) (Adaptor-associated kinase 1)

Protein Class (2)

EnzymesPredicted intracellular proteins

Protein Function (4)

ENZYME proteins:Transferases
Enzymes
Kinases
Predicted intracellular proteins

Ensembl

ENSG00000115977

Entrez Gene Symbol

AAK1

Gene Synonym (2)

DKFZp686K16132KIAA1048

Gene Description

AP2 associated kinase 1

Chromosome

Position

69457997-69674349

Supporting publications (n)

EVMP confidence score

0.50

Fluorescence & Localization1

Function & Pathway7

Protein Function (4)

ENZYME proteins:Transferases
Enzymes
Kinases
Predicted intracellular proteins

Cellular Component (8)

Molecular Function (7)

Biological Process (3)

Reactome (4)

Canonical Pathways (3)

M5887 Naba basement membranes
M3008 Naba ecm glycoproteins
M5884 Naba core matrisome

Mediation Categories (3)

Adhesion and uptake mediationClinical-translation mediationFusion and delivery mediation

Relations & Evidence20

Enzyme-Mediated Modification (4)

4 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
AAK1	STK38	Q15208	S	637	phosphorylation	REACH_ProtMapperSIGNORProtMapper	ProtMapper:22445341 SIGNOR:22445341
AAK1	CDK1	P06493	T	389	phosphorylation	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPProtMapperKEAPhosphoSitePhosphoSite_ProtMapper	KEA:18691976
AAK1	CDK2	P24941	T	640	phosphorylation	KEA	KEA:17570479
AAK1	MAPK8	P45983	T	640	phosphorylation	KEA	KEA:17570479

Ligand-Receptor Signaling (8)

8 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
receptor	receptor	OmniPath	No	Yes	No	No	No
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
plasma_membrane	plasma_membrane	UniProt_location	No	No	No	No	No
plasma_membrane	plasma_membrane	OmniPath	No	No	No	No	No
receptor	receptor	scConnect	No	Yes	No	No	No

Regulatory Interaction Network (5)

5 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
AAK1	Q2M2I8	NUMB	P49757	Yes	Yes	No	phosphoELM_MIMPPhosphoSite_MIMPMIMPiPTMnetSIGNORProtMapperphosphoELMSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	PhosphoSite:18657069 SIGNOR:18657069 phosphoELM:18657069 ProtMapper:18657069
AAK1	Q2M2I8	AP1M1	Q9BXS5	Yes	Yes	No	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetSIGNORProtMapperphosphoELMSIGNOR_ProtMapper	ProtMapper:11877461 SIGNOR:11877461 phosphoELM:11877461
AAK1	Q2M2I8	AP2M1	Q96CW1	Yes	Yes	No	Sparser_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetSIGNORProtMapperELMHINTphosphoELMSIGNOR_ProtMapperPhosphoSite_ProtMapper	SIGNOR:11877461 ProtMapper:34315807 ProtMapper:31905947 phosphoELM:11877457 ELM:11877457 ELM:34315807 ELM:33137362 ProtMapper:11877461 phosphoELM:11877461 HINT:25852190 HINT:33961781
	COMPLEX:O94973_P53680_P63010_Q96CW1	AAK1	Q2M2I8	Yes	Yes	No	SIGNOR	SIGNOR:15496985
STK38	Q15208	AAK1	Q2M2I8	Yes	Yes	No	iPTMnetSIGNORProtMapperSIGNOR_ProtMapperREACH_ProtMapper	ProtMapper:22445341 SIGNOR:22445341

Protein Complex Composition (2)

2 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
	AAK1 CIR1 GEMIN2 RNPS1 SNRNP70 SREK1 SRSF1 SRSF2 U2AF1 U2AF2 ZRSR2	O14893 P08621 P26368 Q01081 Q01130 Q07955 Q15287 Q15696 Q2M2I8 Q86X95 Q8WXA9	1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC8432
	AAK1	Q2M2I8	2	PDB	PDB:4wsqPDB:5l4qPDB:8gmdPDB:9qb5PDB:8gmc

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationUltrafiltration / Tangential Flow Filtration	Mass spectrometry	1	32795414

Sequence, Structure & Domains14

Sequences

Length

961

Mass

103,885

Sequence

MKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINNVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKIITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPAKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKPQAPPSQPLPQTQAKQPQAPPTPQQTPSTQAQGLPAQAQATPQHQQQLFLKQQQQQQQPPPAQQQPAGTFYQQQQAQTQQFQAVHPATQKPAIAQFPVVSQGGSQQQLMQNFYQQQQQQQQQQQQQQLATALHQQQLMTQQAALQQKPTMAAGQQPQPQPAAAPQPAPAQEPAIQAPVRQQPKVQTTPPPAVQGQKVGSLTPPSSPKTQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRTSQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKHPEKLGGSAESLIPGFQSTQGDAFATTSFSAGTAEKRKGGQTVDSGLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLLDCSLLSNPTTDLLEEFAPTAISAPVHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGGHSRNSSGSSESSLPNLARSLLLVDQLIDL

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; Synonyms=AAK1L; IsoId=Q2M2I8-1; Sequence=Displayed; Name=2; Synonyms=AAK1S; IsoId=Q2M2I8-2; Sequence=VSP_039459

Alternative Sequence

823..961; EKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLLDCSLLSNPTTDLLEEFAPTAISAPVHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGGHSRNSSGSSESSLPNLARSLLLVDQLIDL -> GKVIISVSSVMHDMCACFKNDKYLVNQSLGNSPATPEAKAI (in isoform 2)

3D Structural Models

Turn

116..118; 142..144; 261..264; 298..300

Helix

31..34; 81..97; 134..139; 148..167; 179..181; 205..208; 210..220; 223..225; 228..231; 242..257; 266..271; 284..293; 304..315; 339..344

Beta Strand

38..41; 44..55; 58..64; 70..78; 106..113; 120..127; 168..170; 182..184; 190..192

3D Structure

X-ray crystallography (8)

Domain & Motif Annotations

Compositional Bias

1..11; Basic and acidic residues; 12..25; Gly residues; 417..427; Pro residues; 444..476; Low complexity; 483..493; Low complexity; 562..575; Low complexity; 576..588; Pro residues; 615..627; Polar residues; 672..696; Polar residues; 844..860; Polar residues; 931..944; Low complexity

Domain (FT)

46..315; Protein kinase

Region

1..25; Disordered; 327..493; Disordered; 562..632; Disordered; 664..701; Disordered; 823..960; Clathrin-binding domain (CBD); 836..860; Disordered; 921..945; Disordered

Protein Families (2)

Protein kinase superfamily
Ser/Thr protein kinase family

Sequence Similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Clinical Relevance7

Related Diseases (3)

General pain disorder COVID-19 Rheumatoid arthritis

Biomarker

Phase 1; Phase 3; Approved

Drugs (6)

COMPOUND 13 [PMID: 34333981]PILAVAPADIN COMPOUND 6 [PMID: 34333981]COMPOUND 21B [PMID: 31136173]BARICITINIB BMS-911172

Antibody (2)

HPA017931 HPA020289

Interaction Protein (5)

ENSG00000006125ENSG00000017797ENSG00000042753ENSG00000060237ENSG00000159363

Interaction Count

Interaction Dataset (2)

biogrid_opencellintact_biogrid

Supporting Publications3

PMID	Title	Abstract
33531104	MicroRNA-135a in ABCA1-labeled Exosome is a Serum Biomarker Candidate for Alzheimer's Disease.	In the present study, the ABCA1 was used as a label to capture specific exosomes, the level of ABCA1-labeled exosomal microRNA-135a (miR-135a) was evaluated for the diagnosis of Alzheimer's disease (AD), especially in patients with early stages of AD. The level of ABCA1 exosomes harvested from HT-22 cells and neuron culture medium was significantly higher compared to that of RBCs and WBCs ( This study outlines a method to capture specific exosomes and detect them using immunological methods, which is more efficient for early diagnosis of AD.
33763470	ABCA1-Labeled Exosomes in Serum Contain Higher MicroRNA-193b Levels in Alzheimer's Disease.	We aimed to establish a method to determine whether microRNA-193b (miR-193b) levels in ABCA1-labeled serum exosomes might serve as a marker for the diagnosis of Alzheimer's disease. ABCA1-labeled exosomal miR-193b levels were also evaluated in the cerebrospinal fluid (CSF) and serum of APP/PS1 double-transgenic mice, as well as control subjects ( ABCA1 levels of exosomes harvested from the medium of HT-22 cells and neurons were significantly higher than those of RBCs and WBCs ( This study provides a method to capture specific exosomes. Detection of serum exosomes labeled with ABCA1 may facilitate the early diagnosis of AD.
39016173	HIV-1 Nef is carried on the surface of extracellular vesicles.	No abstract available