Protein detail

RGPA2

Ral GTPase-activating protein subunit alpha-2 (250 kDa substrate of Akt) (AS250) (p220)

Entry name RGPA2	UniProt ID Q2PPJ7	EVMP confidence score 0.38
Supporting publications (n) 1	Transmembrane count	Protein classification Predicted intracellular proteinsPredicted membrane proteinsRAS pathway related proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

Ral GTPase-activating protein subunit alpha-2 (250 kDa substrate of Akt) (AS250) (p220)

Protein Class (3)

Predicted intracellular proteinsPredicted membrane proteinsRAS pathway related proteins

Protein Function (2)

Predicted intracellular proteins
RAS pathway related proteins

Ensembl

ENSG00000188559

Entrez Gene Symbol

RALGAPA2

Gene Synonym (5)

AS250C20orf74dJ1049G11.4KIAA1272RapGAPalpha2

Gene Description

Ral GTPase activating protein catalytic subunit alpha 2

Chromosome

Position

20389530-20712644

Supporting publications (n)

EVMP confidence score

0.38

Fluorescence & Localization5

Tissue Specificendometrium 1Cell SpecificAdipocytesSingle-Nuclei Brain Specificchoroid plexus epithelial cellBlood Cell SpecificbasophilBlood Lineage Specificgranulocytes

Function & Pathway7

Protein Function (2)

Predicted intracellular proteins
RAS pathway related proteins

Cellular Component (5)

Molecular Function (3)

Biological Process (3)

KEGG

hsa04014 Ras signaling pathway

Reactome (3)

Mediation Categories (2)

Fusion and delivery mediationReceptor-signaling mediation

Relations & Evidence31

Enzyme-Mediated Modification (9)

9 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
RALGAPA2	AKT1	P31749	S	486	phosphorylation	dbPTMPhosphoSite	dbPTM:16490346
RALGAPA2	AKT1	P31749	S	696	phosphorylation	dbPTM	dbPTM:16490346
RALGAPA2	AKT1	P31749	T	715	phosphorylation	dbPTMPhosphoSite	dbPTM:16490346
RALGAPA2	AKT2	P31751	S	486	phosphorylation	Reactome_ProtMapperSIGNORProtMapper	SIGNOR:21148297
RALGAPA2	AKT2	P31751	T	715	phosphorylation	Reactome_ProtMapperSIGNORProtMapper	SIGNOR:21148297
RALGAPA2	AKT2	P31751	S	696	phosphorylation	Reactome_ProtMapperSIGNORProtMapper	SIGNOR:21148297
RALGAPA2	INS	P01308	S	486	phosphorylation	REACH_ProtMapperProtMapper	ProtMapper:21148297
RALGAPA2	INS	P01308	S	696	phosphorylation	REACH_ProtMapperProtMapper	ProtMapper:21148297
RALGAPA2	INS	P01308	T	715	phosphorylation	REACH_ProtMapperProtMapper	ProtMapper:21148297

Ligand-Receptor Signaling (10)

10 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
ecm	ecm	MatrixDB	Yes	No	No	No	No
ecm	ecm	OmniPath	Yes	No	No	No	No
extracellular	extracellular	OmniPath	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
transmembrane	transmembrane	Ramilowski_location	No	No	No	No	No
transmembrane	transmembrane	OmniPath	No	No	No	No	No
transmembrane	transmembrane_predicted	Phobius	No	No	No	No	No

Regulatory Interaction Network (2)

2 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
AKT2	P31751	RGPA2	Q2PPJ7	Yes	No	Yes	Reactome_ProtMapperSIGNORProtMapper	SIGNOR:21148297
AKT1	P31749	RGPA2	Q2PPJ7	Yes	No	No	PhosphoSite_norefProtMapperdbPTMPhosphoSitePhosphoSite_ProtMapper	PhosphoSite:23386617 dbPTM:16490346 PhosphoSite:16490346

Protein Complex Composition (9)

9 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs
RalGAP2	RALGAPA2 RALGAPB	Q2PPJ7 Q86X10	2:2	SIGNORPDB	SIGNOR:SIGNOR-C469PDB:9qwp
	DPH7 GFOD1 GFOD2 NKIRAS2 RALGAPA1 RALGAPA2 RALGAPB	Q2PPJ7 Q3B7J2 Q6GYQ0 Q86X10 Q9BTV6 Q9NXC2 Q9NYR9	0:0:0:0:0:0:0	hu.MAP2
	CARD10 GFOD1 GFOD2 NKIRAS2 RALGAPA1 RALGAPA2 RALGAPB	Q2PPJ7 Q3B7J2 Q6GYQ0 Q86X10 Q9BWT7 Q9NXC2 Q9NYR9	0:0:0:0:0:0:0	hu.MAP2
	CARD10 FBXO9 GFOD1 GFOD2 RALGAPA1 RALGAPA2 RALGAPB	Q2PPJ7 Q3B7J2 Q6GYQ0 Q86X10 Q9BWT7 Q9NXC2 Q9UK97	0:0:0:0:0:0:0	hu.MAP
	GFOD1 GFOD2 NKIRAS2 RALGAPA1 RALGAPA2 RALGAPB	Q2PPJ7 Q3B7J2 Q6GYQ0 Q86X10 Q9NXC2 Q9NYR9	0:0:0:0:0:0	hu.MAP2
	CARD10 GFOD1 GFOD2 RALGAPA2	Q2PPJ7 Q3B7J2 Q9BWT7 Q9NXC2	0:0:0:0	hu.MAP
	DPH7 RALGAPA2	Q2PPJ7 Q9BTV6	0:0	hu.MAP2
	GFOD1 RALGAPA2	Q2PPJ7 Q9NXC2	0:0	hu.MAP2
	NKIRAS1 RALGAPA2	Q2PPJ7 Q9NYS0	2:2	PDB	PDB:9qu1

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential Ultracentrifugation	Mass spectrometryWestern blotting	4	38731868 30071318 36635400 38136601

Sequence, Structure & Domains9

Sequences

Length

1,873

Mass

210,770

Sequence

MFSRRSHGDVKKSTQKVLDPKKDVLTRLKHLRALLDNVDANDLKQFFETNYSQIYFIFYENFIALENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQALQTNCAEEQVLIFACLVPGFPAVMSSRGPCTLETLINPSPSVADVKIYPEEITPLLPAISGEKIAEDQTCFFLQILLKYMVIQAASLEWKNKENQDTGFKFLFTLFRKYYLPHLFPSFTKLTNIYKPVLDIPHLRPKPVYITTTRDNENIYSTKIPYMAARVVFIKWIVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGAVQERAPELDGGGPTEQDKSHSNSSTLSDRRLSNSSLCSIEEEHRMVYEMVQRILLSTRGYVNFVNEVFHQAFLLPSCEIAVTRKVVQVYRKWILQDKPVFMEEPDRKDVAQEDAEKLGFSETDSKEASSESSGHKRSSSWGRTYSFTSAMSRGCVTEEENTNVKAGVQALLQVFLTNSANIFLLEPCAEVPVLLKEQVDACKAVLIIFRRMIMELTMNKKTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLMVAWIRANLCVYISRELWDDFLGVLSSLTEWEELINEWANIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVLDPQKGTTVGRSFSLSWRSHPDVTEPMRFRSATTSGAPGVEKARNIVRQKATEVEECQQSENAPAAGSGHLTVGQQQQVLRSSSTSDIPEPLCSDSSQGQKAENTQNSSSSEPQPIQENKGHVKREHEGITILVRRSSSPAELDLKDDLQQTQGKCRERQKSESTNSDTTLGCTNEAELSMGPWQTCEEDPELNTPTDVVADADARHWLQLSPTDASNLTDSSECLTDDCSIIAGGSLTGWHPDSAAVLWRRVLGILGDVNNIQSPKIHARVFCYLYELWYKLAKIRDNLAISLDNQSSPSPPVLIPPLRMFASWLFKAATLPNEYKEGKLQAYRLICAMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGFPGFSMLVGDFITAAARVLSTDILTAPRSEAVTVLGSLVCFPNTYQEIPLLQSVPEVNEAITGTEDVKHYLINILLKNATEEPNEYARCIAVCSLGVWICEELAQCTSHPQVKEAINVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKMAEILVATVAFLLPSAEYSSVETDKKFIVSLLLCLLDWCMALPVSVLLHPVSTAVLEEQHSARAPLLDYIYRVLHCCVCGSSTYTQQSHYILTLADLSSTDYDPFLPLANVKSSEPVQYHSSAELGNLLTVEEEKKRRSLELIPLTARMVMAHLVNHLGHYPLSGGPAILHSLVSENHDNAHVEGSELSFEVFRSPNLQLFVFNDSTLISYLQTPTEGPVGGSPVGSLSDVRVIVRDISGKYSWDGKVLYGPLEGCLAPNGRNPSFLISSWHRDTFGPQKDSSQVEEGDDVLDKLLENIGHTSPECLLPSQLNLNEPSLTPCGMNYDQEKEIIEVILRQNAQEDEYIQSHNFDSAMKVTSQGQPSPVEPRGPFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRRGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLVCATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFAAQVFSPSPSYSLSGTD

Alternative Products

Event=Alternative splicing; Named isoforms=3; Name=1; IsoId=Q2PPJ7-1; Sequence=Displayed; Name=2; IsoId=Q2PPJ7-2; Sequence=VSP_025247; Name=3; IsoId=Q2PPJ7-3; Sequence=VSP_025248

Alternative Sequence

1832..1873; FYEERALYLEAIIQNHREVMTFEDFAAQVFSPSPSYSLSGTD -> LYLFALNV (in isoform 2); 1832..1873; FYEERALYLEAIIQNHREVMTFEDFAAQVFSPSPSYSLSGTD -> PRVR (in isoform 3)

3D Structural Models

3D Structure

Electron microscopy (1); X-ray crystallography (1)

Domain & Motif Annotations

Compositional Bias

445..469; Basic and acidic residues; 755..769; Polar residues; 776..799; Polar residues; 801..811; Basic and acidic residues; 825..844; Basic and acidic residues; 845..855; Polar residues

Domain (FT)

1635..1843; Rap-GAP

Region

341..370; Disordered; 445..481; Disordered; 734..856; Disordered

Clinical Relevance1

Antibody (2)

HPA042707 HPA043622

Supporting Publications1

PMID	Title	Abstract
32384937	Alzheimer's disease progression characterized by alterations in the molecular profiles and biogenesis of brain extracellular vesicles.	No abstract available