Protein detail
GRDN
Girdin (Akt phosphorylation enhancer) (APE) (Coiled-coil domain-containing protein 88A) (G alpha-interacting vesicle-associated protein) (GIV) (Girders of actin filament) (Hook-related protein 1) (HkRP1)
Entry name GRDN | UniProt ID | EVMP confidence score 0.38 |
Supporting publications (n) 3 | Transmembrane count | Protein classification Disease related genesHuman disease related genesPredicted intracellular proteins |
EVMP confidence score
Annotation confidence score; open for threshold definitions.
Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40Basic Information11
Protein Names
Girdin (Akt phosphorylation enhancer) (APE) (Coiled-coil domain-containing protein 88A) (G alpha-interacting vesicle-associated protein) (GIV) (Girders of actin filament) (Hook-related protein 1) (HkRP1)
Protein Class (3)
Disease related genesHuman disease related genesPredicted intracellular proteins
Protein Function (3)
- Human disease related genes:Nervous system diseases:Neurodegenerative diseases
- Disease related genes
- Predicted intracellular proteins
Ensembl
Entrez Gene Symbol
Gene Synonym (6)
APEFLJ10392GIVGRDNHkRP1KIAA1212
Gene Description
Coiled-coil domain containing 88A
Chromosome
2
Position
55287842-55419895
Supporting publications (n)
3
EVMP confidence score
0.38
Fluorescence & Localization4
Tissue Specificbone marrowBrain Regional Specificchoroid plexusCell SpecificChoroid plexus epithelial cellsSingle-Nuclei Brain Specificcentral nervous system macrophage
Function & Pathway6
Protein Function (3)
- Human disease related genes:Nervous system diseases:Neurodegenerative diseases
- Disease related genes
- Predicted intracellular proteins
Cellular Component (12)
- GO:0005737 cytoplasm
- GO:0005783 endoplasmic reticulum
- GO:0005794 Golgi apparatus
- GO:0005813 centrosome
- GO:0005814 centriole
- GO:0005829 cytosol
- GO:0005886 plasma membrane
- GO:0016020 membrane
- GO:0030027 lamellipodium
- GO:0030142 COPI-coated Golgi to ER transport vesicle
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Molecular Function (16)
- GO:0001965 G-protein alpha-subunit binding
- GO:0003779 actin binding
- GO:0005080 protein kinase C binding
- GO:0005085 guanyl-nucleotide exchange factor activity
- GO:0005092 GDP-dissociation inhibitor activity
- GO:0005154 epidermal growth factor receptor binding
- GO:0005158 insulin receptor binding
- GO:0008017 microtubule binding
- GO:0031682 G-protein gamma-subunit binding
- GO:0035091 phosphatidylinositol binding
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Biological Process (3)
Reactome (4)
Mediation Categories (2)
Fusion and delivery mediationReceptor-signaling mediation
Relations & Evidence26
Enzyme-Mediated Modification (14)
14 records.
| Substrate Gene Symbol | Enzyme Gene Symbol | Enzyme UniProt ID | Residue Type | Residue Offset | Modification | Database | References |
|---|---|---|---|---|---|---|---|
| CCDC88A | AKT1 | P31749 | S | 1,417 | phosphorylation | SIGNORProtMapperHPRDdbPTMKEASIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper | dbPTM:16139227HPRD:16139227HPRD:20068231KEA:16139227SIGNOR:16139227ProtMapper:16139227 |
| CCDC88A | AKT1 | P31749 | S | 1,416 | phosphorylation | MIMPHPRD_MIMPPhosphoSite_MIMP | |
| CCDC88A | PRKCQ | Q04759 | S | 1,690 | phosphorylation | REACH_ProtMapperPhosphoSitePhosphoSite_ProtMapperProtMapper | ProtMapper:26378251 |
| CCDC88A | SRC | P12931 | Y | 1,799 | phosphorylation | Sparser_ProtMapperPhosphoSitePhosphoSite_ProtMapperProtMapper | ProtMapper:25707853 |
| CCDC88A | SRC | P12931 | Y | 1,765 | phosphorylation | PhosphoSiteSparser_ProtMapperProtMapper | ProtMapper:25707853 |
| CCDC88A | MAPK1 | P28482 | S | 233 | phosphorylation | PhosphoSitePhosphoSite_ProtMapperProtMapper | |
| CCDC88A | MAPK1 | P28482 | S | 237 | phosphorylation | PhosphoSitePhosphoSite_ProtMapperProtMapper | |
| CCDC88A | CDK5 | Q00535 | S | 1,675 | phosphorylation | PhosphoSitePhosphoSite_ProtMapperProtMapper | |
| CCDC88A | EGFR | P00533 | Y | 1,799 | phosphorylation | PhosphoSiteSparser_ProtMapperProtMapper | ProtMapper:25707853 |
| CCDC88A | EGFR | P00533 | Y | 1,765 | phosphorylation | PhosphoSiteSparser_ProtMapperProtMapper | ProtMapper:25707853 |
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Ligand-Receptor Signaling (7)
7 records.
| Category | Parent | Database | Transmitter | Receiver | Secreted | Plasma Membrane (Transmembrane) | Plasma Membrane (Peripheral) |
|---|---|---|---|---|---|---|---|
| intracellular | intracellular | LOCATE | No | No | No | No | No |
| intracellular | intracellular | ComPPI | No | No | No | No | No |
| intracellular | intracellular | GO_Intercell | No | No | No | No | No |
| intracellular | intracellular | UniProt_location | No | No | No | No | No |
| intracellular | intracellular | OmniPath | No | No | No | No | No |
| plasma_membrane | plasma_membrane | UniProt_location | No | No | No | No | No |
| plasma_membrane | plasma_membrane | OmniPath | No | No | No | No | No |
Regulatory Interaction Network (4)
4 records.
| Source Protein Symbol | Source UniProt ID | Target Protein Symbol | Target UniProt ID | Is Directed | Is Stimulation | Is Inhibition | Database | References |
|---|---|---|---|---|---|---|---|---|
| AKT1 | P31749 | GRDN | Q3V6T2 | Yes | No | No | PhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetPhosphoPointSIGNORProtMapperHPRDHINTPhosphoSiteKEAdbPTMHPRD_KEAInnateDBSIGNOR_ProtMapperLit-BM-17HPRD-phosPhosphoSite_ProtMapper | Lit-BM-17:16139227PhosphoSite:25707853PhosphoSite:24662825ProtMapper:16139227InnateDB:16139227HPRD-phos:16139227dbPTM:16139227HINT:15753085HPRD-phos:20068231PhosphoSite:25732845PhosphoSite:30935690HINT:16139227KEA:16139227SIGNOR:16139227PhosphoSite:16139227HPRD:16139227Lit-BM-17:15753085ProtMapper:20068231PhosphoSite:21954290 |
| KPCT | Q04759 | GRDN | Q3V6T2 | Yes | No | No | iPTMnetProtMapperREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper | PhosphoSite:27621449ProtMapper:26378251PhosphoSite:23509302PhosphoSite:31363053 |
| EGFR | P00533 | GRDN | Q3V6T2 | Yes | No | No | Sparser_ProtMapperProtMapperBioGRIDREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper | BioGRID:15882442PhosphoSite:32307399ProtMapper:25707853 |
| CDK5 | Q00535 | GRDN | Q3V6T2 | Yes | No | No | iPTMnetPhosphoSitePhosphoSite_ProtMapperProtMapper | PhosphoSite:27864364PhosphoSite:26286990 |
Isolation & Detection Technology (1)
1 record.
| EV Isolation Method | Detection Method | Number of References | References |
|---|---|---|---|
| Differential UltracentrifugationUltrafiltration / Tangential Flow Filtration | Data-Dependent Acquisition LC-MS/MS | 1 | 32795414 |
Sequence, Structure & Domains16
Sequences
Length
1,871
Mass
216,042
Sequence
MENEIFTPLLEQFMTSPLVTWVKTFGPLAAGNGTNLDEYVALVDGVFLNQVMLQINPKLESQRVNKKVNNDASLRMHNLSILVRQIKFYYQETLQQLIMMSLPNVLIIGKNPFSEQGTEEVKKLLLLLLGCAVQCQKKEEFIERIQGLDFDTKAAVAAHIQEVTHNQENVFDLQWMEVTDMSQEDIEPLLKNMALHLKRLIDERDEHSETIIELSEERDGLHFLPHASSSAQSPCGSPGMKRTESRQHLSVELADAKAKIRRLRQELEEKTEQLLDCKQELEQMEIELKRLQQENMNLLSDARSARMYRDELDALREKAVRVDKLESEVSRYKERLHDIEFYKARVEELKEDNQVLLETKTMLEDQLEGTRARSDKLHELEKENLQLKAKLHDMEMERDMDRKKIEELMEENMTLEMAQKQSMDESLHLGWELEQISRTSELSEAPQKSLGHEVNELTSSRLLKLEMENQSLTKTVEELRTTVDSVEGNASKILKMEKENQRLSKKVEILENEIVQEKQSLQNCQNLSKDLMKEKAQLEKTIETLRENSERQIKILEQENEHLNQTVSSLRQRSQISAEARVKDIEKENKILHESIKETSSKLSKIEFEKRQIKKELEHYKEKGERAEELENELHHLEKENELLQKKITNLKITCEKIEALEQENSELERENRKLKKTLDSFKNLTFQLESLEKENSQLDEENLELRRNVESLKCASMKMAQLQLENKELESEKEQLKKGLELLKASFKKTERLEVSYQGLDIENQRLQKTLENSNKKIQQLESELQDLEMENQTLQKNLEELKISSKRLEQLEKENKSLEQETSQLEKDKKQLEKENKRLRQQAEIKDTTLEENNVKIGNLEKENKTLSKEIGIYKESCVRLKELEKENKELVKRATIDIKTLVTLREDLVSEKLKTQQMNNDLEKLTHELEKIGLNKERLLHDEQSTDDSRYKLLESKLESTLKKSLEIKEEKIAALEARLEESTNYNQQLRQELKTVKKNYEALKQRQDEERMVQSSPPISGEDNKWERESQETTRELLKVKDRLIEVERNNATLQAEKQALKTQLKQLETQNNNLQAQILALQRQTVSLQEQNTTLQTQNAKLQVENSTLNSQSTSLMNQNAQLLIQQSSLENENESVIKEREDLKSLYDSLIKDHEKLELLHERQASEYESLISKHGTLKSAHKNLEVEHRDLEDRYNQLLKQKGQLEDLEKMLKVEQEKMLLENKNHETVAAEYKKLCGENDRLNHTYSQLLKETEVLQTDHKNLKSLLNNSKLEQTRLEAEFSKLKEQYQQLDITSTKLNNQCELLSQLKGNLEEENRHLLDQIQTLMLQNRTLLEQNMESKDLFHVEQRQYIDKLNELRRQKEKLEEKIMDQYKFYDPSPPRRRGNWITLKMRKLIKSKKDINRERQKSLTLTPTRSDSSEGFLQLPHQDSQDSSSVGSNSLEDGQTLGTKKSSMVALKRLPFLRNRPKDKDKMKACYRRSMSMNDLVQSMVLAGQWTGSTENLEVPDDISTGKRRKELGAMAFSTTAINFSTVNSSAGFRSKQLVNNKDTTSFEDISPQGVSDDSSTGSRVHASRPASLDSGRTSTSNSNNNASLHEVKAGAVNNQSRPQSHSSGEFSLLHDHEAWSSSGSSPIQYLKRQTRSSPVLQHKISETLESRHHKIKTGSPGSEVVTLQQFLEESNKLTSVQIKSSSQENLLDEVMKSLSVSSDFLGKDKPVSCGLARSVSGKTPGDFYDRRTTKPEFLRPGPRKTEDTYFISSAGKPTPGTQGKIKLVKESSLSRQSKDSNPYATLPRASSVISTAEGTTRRTSIHDFLTKDSRLPISVDSPPAAADSNTTAASNVDKVQESRNSKSRSREQQSS
Alternative Products
Event=Alternative splicing; Named isoforms=5; Name=1; IsoId=Q3V6T2-1; Sequence=Displayed; Name=2; IsoId=Q3V6T2-2; Sequence=VSP_052409; Name=3; IsoId=Q3V6T2-3; Sequence=VSP_040129; Name=4; IsoId=Q3V6T2-4; Sequence=VSP_040129, VSP_052409; Name=5; IsoId=Q3V6T2-5; Sequence=VSP_040129, VSP_044943
Alternative Sequence
952; Missing (in isoform 3, isoform 4 and isoform 5); 1463..1491; MVALKRLPFLRNRPKDKDKMKACYRRSMS -> T (in isoform 2 and isoform 4); 1733..1806; Missing (in isoform 5)
3D Structural Models
Helix
1683..1690
Beta Strand
1679..1682
3D Structure
X-ray crystallography (1)
Domain & Motif Annotations
Compositional Bias
1026..1035; Basic and acidic residues; 1407..1416; Basic and acidic residues; 1417..1430; Polar residues; 1445..1459; Polar residues; 1559..1578; Polar residues; 1743..1763; Basic and acidic residues; 1787..1799; Polar residues; 1807..1818; Polar residues; 1820..1830; Basic and acidic residues; 1838..1851; Low complexity; 1854..1871; Basic and acidic residues
Motif
1672..1702; GBA
Coiled Coil
196..425; 458..1385
Domain (CC)
The GBA (G-alpha binding and activating) motif mediates binding to the alpha subunits of guanine nucleotide-binding proteins (G proteins).; DOMAIN: In the presence of tyrosine-autophosphorylated growth factor receptors, the C-terminus folds into an SH2-like region which promotes the stable recruitment of CCDC88A to the growth factor receptors. The SH2-like region is phosphorylated by the growth factor receptors prior to completion of folding.
Domain (FT)
12..132; Calponin-homology (CH)
Region
816..842; Disordered; 1010..1035; Disordered; 1390..1408; Phosphoinositide-binding; 1407..1459; Disordered; 1559..1601; Disordered; 1713..1823; SH2-like; required for interaction with growth factor receptors; 1736..1871; Disordered
Protein Families
CCDC88 family
Sequence Similarities
Belongs to the CCDC88 family.
Clinical Relevance2
Supporting Publications3
| PMID | Title | Abstract |
|---|---|---|
| 32111925 | Rigorous characterization of urinary extracellular vesicles (uEVs) in the low centrifugation pellet - a neglected source for uEVs. | No abstract available |
| 32795414 | Extracellular Vesicle and Particle Biomarkers Define Multiple Human Cancers. | Among traditional exosome markers, CD9, HSPA8, ALIX, and HSP90AB1 represent pan-EVP markers, while ACTB, MSN, and RAP1B are novel pan-EVP markers. |
| 41216884 | Extracellular Vesicles From Multiple Sclerosis White Matter Exhibit Synaptic, Mitochondrial, Complement and Ageing-Related Pathway Dysregulation. | No abstract available |