Protein detail

KCNT1

Potassium channel subfamily T member 1 (KCa4.1) (KNa1.1) (Sodium and chloride-activated ATP-sensitive potassium channel Slo2.2)

Entry name KCNT1	UniProt ID Q5JUK3	EVMP confidence score 0.50
Supporting publications (n) 1	Transmembrane count 6	Protein classification Disease related genesHuman disease related genesPotential drug targetsPredicted membrane proteinsTransportersVoltage-gated ion channels

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information13

Protein Names

Potassium channel subfamily T member 1 (KCa4.1) (KNa1.1) (Sodium and chloride-activated ATP-sensitive potassium channel Slo2.2)

Protein Class (6)

Disease related genesHuman disease related genesPotential drug targetsPredicted membrane proteinsTransportersVoltage-gated ion channels

Protein Function (5)

Human disease related genes:Nervous system diseases:Epilepsy
Potential drug targets
Voltage-gated ion channels:Calcium-Activated Potassium Channels
Transporters:Transporter channels and pores
Disease related genes

Transmembrane

94..126; Helical; Name=Segment S1; 154..178; Helical; Name=Segment S2; 193..208; Helical; Name=Segment S3; 216..233; Helical; Name=Segment S4; 247..274; Helical; Name=Segment S5; 305..338; Helical; Name=Segment S6

Transmembrane Count

Ensembl

ENSG00000107147

Entrez Gene Symbol

KCNT1

Gene Synonym (4)

KCa4.1KIAA1422SLACKSlo2.2

Gene Description

Potassium sodium-activated channel subfamily T member 1

Chromosome

Position

135702185-135795508

Supporting publications (n)

EVMP confidence score

0.50

Fluorescence & Localization7

Cell SpecificExtravillous trophoblastsSingle-Nuclei Brain Specificendothelial cellBlood Cell SpecificMAIT T-cellBlood Lineage SpecificT-cellsSecretome LocationSecreted to bloodSecretome FunctionReceptor

Function & Pathway6

Protein Function (5)

Human disease related genes:Nervous system diseases:Epilepsy
Potential drug targets
Voltage-gated ion channels:Calcium-Activated Potassium Channels
Transporters:Transporter channels and pores
Disease related genes

Cellular Component

GO:0005886 plasma membrane

Molecular Function (2)

Biological Process (3)

Canonical Pathways

M159 Pid amb2 neutrophils pathway

Mediation Categories (2)

Clinical-translation mediationFusion and delivery mediation

Relations & Evidence19

Ligand-Receptor Signaling (17)

17 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
transmembrane	transmembrane	OmniPath	No	No	No	No	No
peripheral	peripheral	UniProt_topology	No	No	No	No	No
peripheral	peripheral	OmniPath	No	No	No	No	No
plasma_membrane	plasma_membrane	UniProt_location	No	No	No	No	No
plasma_membrane	plasma_membrane	OmniPath	No	No	No	No	No
receptor	receptor	scConnect	No	Yes	No	No	No
transmembrane	transmembrane_predicted	Phobius	No	No	No	No	No

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Protein Complex Composition (1)

1 record.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
	KCNT1	Q5JUK3	4	PDB	PDB:8hirPDB:8hkqPDB:8hk6PDB:8hkkPDB:8hkmPDB:8hkf

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential Ultracentrifugation	Mass spectrometry	1	28986585

Sequence, Structure & Domains15

Sequences

Length

1,230

Mass

138,343

Sequence

MARAKLPRSPSEGKAGPGGAPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRLPVEDFSLDSSLSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYSFNDSSSEINWAPILWVERKMTLWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFVLEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGENLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRMYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKRKKRAFSGQGLHEGPARLPVHSIIASMGTVAMDLQGTEHRPTQSGGGGGGSKLALPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRKELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKITEGDLWIRTYGRLFQKLCSSSAEIPIGIYRTESHVFSTSESQISVNVEDCEDTREVKGPWGSRAGTGGSSQGRHTGGGDPAEHPLLRRKSLQWARRLSRKAPKQAGRAAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHQNTLSYVLINPPPDTRLEPSDIVYLIRSDPLAHVASSSQSRKSSCSHKLSSCNPETRDETQL

Alternative Products

Event=Alternative splicing; Named isoforms=4; Name=1; IsoId=Q5JUK3-1; Sequence=Displayed; Name=2; IsoId=Q5JUK3-2; Sequence=VSP_015470, VSP_015471; Name=3; IsoId=Q5JUK3-3; Sequence=VSP_015470, VSP_015471, VSP_044476; Name=4; IsoId=Q5JUK3-4; Sequence=VSP_055700, VSP_055701, VSP_015471

Alternative Sequence

1..66; MARAKLPRSPSEGKAGPGGAPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRLPVEDFSLDSSLSQ -> MPLPDGARTPGGVCREARGGGYTNRTFEFDDGQCAPRRPCAGDGALLDTAGFKMSDLDSEVLPLPPRYRFRDLLLGDPSFQNDDR (in isoform 2 and isoform 3); 1..66; MARAKLPRSPSEGKAGPGGAPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRLPVEDFSLDSSLSQ -> MPLPDGARTPGGVCREARGGGYTNRTFEFDDGQCAPR (in isoform 4); 266; G -> GGCR (in isoform 4); 1033; E -> EPHDLRAQ (in isoform 2, isoform 3 and isoform 4); 1142..1162; Missing (in isoform 3)

3D Structural Models

Turn

187..189; 274..278; 522..525; 551..553; 611..614; 963..965

Helix

72..75; 78..86; 92..116; 120..122; 146..149; 155..178; 193..208; 211..214; 219..221; 222..234; 249..273; 282..293; 306..337; 346..352; 364..374; 378..380; 395..402; 404..407; 420..425; 428..430; 445..462; 475..480; 490..503; 507..514; 529..537; 554..556; 561..572; 615..624; 757..760; 774..777; 793..803; 809..811; 825..831; 847..853; 855..857; 868..871; 875..879; 880..892; 905..910; 918..932; 939..941; 943..946; 954..962; 969..976; 995..997; 1003..1013; 1103..1116; 1119..1134

Beta Strand

124..129; 138..140; 184..186; 299..301; 354..358; 384..388; 410..413; 434..437; 440..443; 464..466; 468..474; 484..489; 541..547; 575..580; 587..589; 602..609; 731..733; 748..750; 761..764; 783..790; 815..821; 837..841; 844..846; 858..863; 897..903; 949..952; 987..993; 1017..1026; 1171..1178; 1189..1195

3D Structure

Electron microscopy (6)

Domain & Motif Annotations

Compositional Bias

1057..1072; Gly residues; 1204..1219; Low complexity

Domain (CC)

The cytoplasmic gating ring domain of the closed KCNT1 channel harbors multiple K(+) and Zn(2+) sites, which stabilize the channel in the closed conformation. Under low-Na(+) conditions, the abundant cytoplasmic K(+) ions stabilize the gating ring domain in a closed conformation. KCNT1 contains at least two Na(+)-sensitive sites in the RCKs domain where Na(+) binding induces expansion and rotation of the gating ring that opens the inner gate.; DOMAIN: The cytoplasmic N-terminal domain facilitates the localization of heteromeric KCNT1/KCNT2 channels to the plasma membrane.

Domain (FT)

352..488; RCK N-terminal 1; 781..921; RCK N-terminal 2

Region

1..37; Disordered; 660..689; Disordered; 1048..1078; Disordered; 1204..1230; Disordered

Protein Families (3)

Potassium channel family
Calcium-activated (TC 1.A.1.3) subfamily
KCa4.1/KCNT1 sub-subfamily

Sequence Similarities

Belongs to the potassium channel family. Calcium-activated (TC 1.A.1.3) subfamily. KCa4.1/KCNT1 sub-subfamily.

Clinical Relevance5

Disease Involvement (2)

Disease variantEpilepsy

Related Diseases (2)

Acute myeloid leukaemia Hodgkin lymphoma

Biomarker

Phase 2

Drugs (5)

PHORBOL 12-MYRISTATE 13-ACETATE LOXAPINE QUINIDINE NICLOSAMIDE CLOFILIUM

Antibody (2)

HPA059880 HPA060577

Supporting Publications1

PMID	Title	Abstract
39222826	CCL2/CCR2 axis promotes perineural invasion of salivary adenoid cystic carcinoma via ITGβ5-mediated nerve-tumor interaction.	High levels of ITGβ5 in tissues or plasma exosomes were significantly correlated with CCL2 and CCR2 expression in the tissues and associated with PNI and poor prognosis of SACC cases.