Protein detail

M3K21

Mitogen-activated protein kinase kinase kinase 21 (EC 2.7.11.25) (Mitogen-activated protein kinase kinase kinase MLK4) (Mixed lineage kinase 4)

Entry name M3K21	UniProt ID Q5TCX8	EVMP confidence score 0.38
Supporting publications (n) 2	Transmembrane count	Protein classification EnzymesPredicted intracellular proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

Mitogen-activated protein kinase kinase kinase 21 (EC 2.7.11.25) (Mitogen-activated protein kinase kinase kinase MLK4) (Mixed lineage kinase 4)

Protein Class (2)

EnzymesPredicted intracellular proteins

Protein Function (4)

ENZYME proteins:Transferases
Enzymes
Kinases:STE Ser/Thr protein kinases
Predicted intracellular proteins

Ensembl

ENSG00000143674

Entrez Gene Symbol

MAP3K21

Gene Synonym (2)

KIAA1804MLK4

Gene Description

Mitogen-activated protein kinase kinase kinase 21

Chromosome

Position

233327724-233385148

Supporting publications (n)

EVMP confidence score

0.38

Fluorescence & Localization1

Cell SpecificBreast lactating cells

Function & Pathway6

Protein Function (4)

ENZYME proteins:Transferases
Enzymes
Kinases:STE Ser/Thr protein kinases
Predicted intracellular proteins

Cellular Component (2)

Molecular Function (6)

Biological Process (3)

KEGG

hsa04010 MAPK signaling pathway

Mediation Categories

Other mediation

Relations & Evidence8

Ligand-Receptor Signaling (3)

3 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No

Regulatory Interaction Network (3)

3 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
M3K21	Q5TCX8	IKKA	O15111	Yes	No	Yes	SIGNOR	SIGNOR:26859459
M3K21	Q5TCX8	MP2K1	Q02750	Yes	Yes	No	SIGNOR_ProtMapperiPTMnetSIGNORProtMapper	SIGNOR:23319808 ProtMapper:23319808
M3K21	Q5TCX8	I17RB	Q9NRM6	Yes	No	No	PhosphoSitePhosphoSite_ProtMapperProtMapper	PhosphoSite:33658352

Protein Complex Composition (1)

1 record.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
	GPBP1 MAP3K21 SLX1B	Q5TCX8 Q86WP2 Q9BQ83	0:0:0	hu.MAP2

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential Ultracentrifugation	Mass spectrometry	1	38225453

Sequence, Structure & Domains14

Sequences

Length

1,036

Mass

113,957

Sequence

MALRGAAGATDTPVSSAGGAPGGSASSSSTSSGGSASAGAGLWAALYDYEARGEDELSLRRGQLVEVLSQDAAVSGDEGWWAGQVQRRLGIFPANYVAPCRPAASPAPPPSRPSSPVHVAFERLELKELIGAGGFGQVYRATWQGQEVAVKAARQDPEQDAAAAAESVRREARLFAMLRHPNIIELRGVCLQQPHLCLVLEFARGGALNRALAAANAAPDPRAPGPRRARRIPPHVLVNWAVQIARGMLYLHEEAFVPILHRDLKSSNILLLEKIEHDDICNKTLKITDFGLAREWHRTTKMSTAGTYAWMAPEVIKSSLFSKGSDIWSYGVLLWELLTGEVPYRGIDGLAVAYGVAVNKLTLPIPSTCPEPFAKLMKECWQQDPHIRPSFALILEQLTAIEGAVMTEMPQESFHSMQDDWKLEIQQMFDELRTKEKELRSREEELTRAALQQKSQEELLKRREQQLAEREIDVLERELNILIFQLNQEKPKVKKRKGKFKRSRLKLKDGHRISLPSDFQHKITVQASPNLDKRRSLNSSSSSPPSSPTMMPRLRAIQLTSDESNKTWGRNTVFRQEEFEDVKRNFKKKGCTWGPNSIQMKDRTDCKERIRPLSDGNSPWSTILIKNQKTMPLASLFVDQPGSCEEPKLSPDGLEHRKPKQIKLPSQAYIDLPLGKDAQRENPAEAESWEEAASANAATVSIEMTPTNSLSRSPQRKKTESALYGCTVLLASVALGLDLRELHKAQAAEEPLPKEEKKKREGIFQRASKSRRSASPPTSLPSTCGEASSPPSLPLSSALGILSTPSFSTKCLLQMDSEDPLVDSAPVTCDSEMLTPDFCPTAPGSGREPALMPRLDTDCSVSRNLPSSFLQQTCGNVPYCASSKHRPSHHRRTMSDGNPTPTGATIISATGASALPLCPSPAPHSHLPREVSPKKHSTVHIVPQRRPASLRSRSDLPQAYPQTAVSQLAQTACVVGRPGPHPTQFLAAKERTKSHVPSLLDADVEGQSRDYTVPLCRMRSKTSRPSIYELEKEFLS

Alternative Products

Event=Alternative splicing; Named isoforms=3; Name=1; Synonyms=MLK4beta; IsoId=Q5TCX8-1; Sequence=Displayed; Name=2; Synonyms=MLK4alpha; IsoId=Q5TCX8-2; Sequence=VSP_051733, VSP_051734; Name=3; IsoId=Q5TCX8-3; Sequence=VSP_051731, VSP_051732

Alternative Sequence

1..554; Missing (in isoform 3); 555..559; RAIQL -> MFFLV (in isoform 3); 559..570; LTSDESNKTWGR -> CELSALPRGLLC (in isoform 2); 571..1036; Missing (in isoform 2)

3D Structural Models

Turn

343..346

Helix

121..123; 165..175; 208..212; 234..252; 266..268; 313..316; 323..339; 349..357; 373..380; 391..399; 411..436

Beta Strand

125..129; 138..143; 146..152; 186..190; 197..201; 255..257; 269..273; 285..287

3D Structure

X-ray crystallography (1)

Domain & Motif Annotations

Compositional Bias

15..36; Low complexity; 748..763; Basic and acidic residues

Domain (FT)

38..102; SH3; 124..401; Protein kinase

Region

1..36; Disordered; 425..446; Leucine-zipper 1; 460..481; Leucine-zipper 2; 517..551; Disordered; 748..791; Disordered; 923..954; Disordered

Protein Families (3)

Protein kinase superfamily
STE Ser/Thr protein kinase family
MAP kinase kinase kinase subfamily

Sequence Similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

Clinical Relevance4

Antibody

HPA007636

Interaction Protein (8)

ENSG00000096060ENSG00000108953ENSG00000121905ENSG00000128245ENSG00000130758ENSG00000164924ENSG00000166913ENSG00000170027

Interaction Count

Interaction Dataset (4)

biogrid_opencellbiogrid_bioplexintact_biogridintact_biogrid_opencell

Supporting Publications2

PMID	Title	Abstract
33919183	Multiple-Cycle Polymeric Extracellular Vesicle Precipitation and Its Evaluation by Targeted Mass Spectrometry.	No abstract available
40311616	Integrative proteomic profiling of tumor and plasma extracellular vesicles identifies a diagnostic biomarker panel for colorectal cancer.	No abstract available