Protein detail

MRCKA

Serine/threonine-protein kinase MRCK alpha (EC 2.7.11.1) (CDC42-binding protein kinase alpha) (DMPK-like alpha) (Myotonic dystrophy kinase-related CDC42-binding kinase alpha) (MRCK alpha) (Myotonic dystrophy protein kinase-like alpha)

Entry name MRCKA	UniProt ID Q5VT25	EVMP score 0.38
Frequency 2	Transmembrane count	Protein classification EnzymesPredicted intracellular proteins

EVMP score: annotation confidence score.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information

Protein Names

Protein Class

EnzymesPredicted intracellular proteins

Protein Function

ENZYME proteins:Transferases
Enzymes
Kinases:AGC Ser/Thr protein kinases
Predicted intracellular proteins

Ensembl

ENSG00000143776

Entrez Gene Symbol

CDC42BPA

Gene Synonym

FLJ23347KIAA0451MRCKMRCKAPK428

Gene Description

CDC42 binding protein kinase alpha

Chromosome

Position

226989865-227318492

Frequency

EVMP Score

0.38

Fluorescence & Localization

Tissue Specificheart muscleCell SpecificCardiomyocytesSingle-Nuclei Brain Specificchoroid plexus epithelial cellBlood Cell SpecificbasophilBlood Lineage Specificgranulocytes

Function & Pathway

Protein Function

ENZYME proteins:Transferases
Enzymes
Kinases:AGC Ser/Thr protein kinases
Predicted intracellular proteins

Cellular Component

Molecular Function

Biological Process

Reactome

Mediation Categories

Clinical-translation mediationFusion and delivery mediationReceptor-signaling mediation

Relations & Evidence

Enzyme-Mediated Modification

3 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
CDC42BPA	CDC42	P60953	T	240	phosphorylation	NCI-PID_ProtMapperProtMapper	ProtMapper:15882626
CDC42BPA	CDC42	P60953	S	234	phosphorylation	NCI-PID_ProtMapperProtMapper	ProtMapper:15882626
CDC42BPA	CDC42	P60953	S	222	phosphorylation	NCI-PID_ProtMapperProtMapper	ProtMapper:15882626

Ligand-Receptor Signaling

6 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
receptor	receptor	OmniPath	No	Yes	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
receptor	receptor	scConnect	No	Yes	No	No	No

Regulatory Interaction Network

6 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
MRCKA	Q5VT25	LIMK1	P53667	Yes	Yes	No	HPRD_MIMPSIGNORProtMapperPhosphoSite_KEAphosphoELM_KEAHPRDHPRD-phosphosphoELM_MIMPPhosphoSite_MIMPMIMPiPTMnetPhosphoPointKEAHPRD_KEAphosphoELMSIGNOR_ProtMapperACSNSPIKE_LCSPIKE	ProtMapper:11340065 SPIKE:11340065 ProtMapper:10652353 HPRD-phos:10559936 ACSN:17522712 HPRD-phos:11340065 HPRD:10652353 KEA:11340065 ACSN:11340065 HPRD:10559936 KEA:10559936 HPRD-phos:10652353 HPRD:11340065 ACSN:11950587 KEA:10652353 ACSN:22154077 ACSN:10461188 phosphoELM:11340065 SPIKE_LC:11340065 ACSN:10559936 ACSN:11413130 ACSN:10613909 ProtMapper:10559936 ACSN:11018042 SIGNOR:11340065
MRCKA	Q5VT25	LIMK2	P53671	Yes	Yes	No	HPRD_MIMPSIGNORProtMapperRLIMS-P_ProtMapperPhosphoSite_KEAphosphoELM_KEAHPRDphosphoELM_MIMPPhosphoSite_MIMPMIMPiPTMnetPhosphoPointKEAHPRD_KEAphosphoELMSIGNOR_ProtMapperREACH_ProtMapperACSNSPIKE_LCSPIKE	KEA:11171090 KEA:11340065 ProtMapper:11340065 ACSN:11340065 SPIKE:11340065 ACSN:10613909 phosphoELM:11340065 SPIKE_LC:11340065 ACSN:10559936 HPRD:11340065 ACSN:11950587 ACSN:11413130 ACSN:10461188 KEA:11018042 SIGNOR:11340065
MRCKA	Q5VT25	PP12C	Q9BZL4	Yes	No	Yes	PhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetSIGNORProtMapperHPRDKEAHPRD_KEASIGNOR_ProtMapperHPRD-phos	KEA:11399775 HPRD:11399775 HPRD-phos:18077418 ProtMapper:18077418 ProtMapper:11399775 SIGNOR:11399775 HPRD-phos:11399775
MRCKA	Q5VT25	MYL9	P24844	Yes	Yes	No	WangphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetSIGNORProtMapperHPRDSIGNOR_ProtMapperPhosphoSite_ProtMapper	ProtMapper:19851336 HPRD:11283256 SIGNOR:19851336
CDC42	P60953	MRCKA	Q5VT25	Yes	Yes	No	WangNCI-PID_ProtMapperSIGNORProtMapperHINTACSNIntActSPIKE_LCLit-BM-17	ACSN:10934474 SPIKE_LC:17145710 ACSN:11340065 IntAct:9418861 ACSN:11950587 ACSN:10461188 HINT:9418861 HINT:35271311 Lit-BM-17:9418861 ACSN:18719708 IntAct:20936779 HINT:15194684 ACSN:10559936 Lit-BM-17:15194684 ACSN:11248548 ACSN:11413130 ACSN:10613909 SIGNOR:9418861 ACSN:15003621 ACSN:15020669 IntAct:15194684 Lit-BM-17:20936779 ProtMapper:15882626
MRCKA	Q5VT25	MLRV	P10916	Yes	Yes	No	PhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetSIGNORProtMapperHPRDKEAHPRD_KEASIGNOR_ProtMapperHPRD-phos	HPRD:9418861 HPRD-phos:9418861 KEA:9418861 SIGNOR:9418861 ProtMapper:9418861

Protein Complex Composition

3 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
Mob2p/Cbk1p complex	CDC42BPA CDC42BPG MOB2 STK38 STK38L	Q15208 Q5VT25 Q6DT37 Q70IA6 Q9Y2H1	1:1:1:1:1	Compleat	Compleat:HC169	12196508
	CDC42BPA MYL2 MYL9 MYO18A PPIA PPP1R12C	P10916 P24844 P62937 Q5VT25 Q92614 Q9BZL4	1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC5301
	CDC42BPA LURAP1L	Q5VT25 Q8IV03	0:0	hu.MAP2

Isolation & Detection Technology

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationUltrafiltration / Tangential Flow FiltrationSize Exclusion Chromatography	Mass spectrometry	5	28211531 33169421 31414377 28914500 32331267

Sequence, Structure & Domains

Sequences

Length

1,732

Mass

197,307

Sequence

MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFERSPSCTPASKGRRTVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Alternative Products

Event=Alternative splicing; Named isoforms=6; Comment=Additional isoforms seem to exist. They arise due to a two alternate splice sites, the first site involves splicing of exons 21-24 while the second site involves exons 36-40.; Name=1; IsoId=Q5VT25-1; Sequence=Displayed; Name=2; IsoId=Q5VT25-2; Sequence=VSP_051861, VSP_051863; Name=3; IsoId=Q5VT25-3; Sequence=VSP_051859, VSP_051860; Name=4; IsoId=Q5VT25-4; Sequence=VSP_051862; Name=5; IsoId=Q5VT25-5; Sequence=VSP_051860; Name=6; IsoId=Q5VT25-6; Sequence=VSP_051860, VSP_035286

Alternative Sequence

550..630; Missing (in isoform 3); 969..1009; Missing (in isoform 4); 969..981; Missing (in isoform 3, isoform 5 and isoform 6); 969; R -> TDPVENTYVWNPSVKFHIQSRST (in isoform 2); 973..981; CTPASKGRR -> TSSEAEPVK (in isoform 2); 1597; M -> MPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLR (in isoform 6)

3D Structural Models

Domain & Motif Annotations

Compositional Bias

1604..1619; Polar residues; 1625..1640; Low complexity; 1665..1674; Low complexity; 1697..1707; Low complexity

Coiled Coil

437..820; 880..943

Zinc Finger

1012..1062; Phorbol-ester/DAG-type

Domain (FT)

77..343; Protein kinase; 344..414; AGC-kinase C-terminal; 1082..1201; PH; 1227..1499; CNH; 1571..1584; CRIB

Region

968..1003; Disordered; 1591..1732; Disordered

Protein Families

Protein kinase superfamily
AGC Ser/Thr protein kinase family
DMPK subfamily

Sequence Similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.

Clinical Relevance

Drug Targets

Literature-reported target

Drugs

PHA-767491 RKI-1447 ALSTERPAULLONE LAUROGUADINE GSK-269962A NVP-TAE684 KENPAULLONE DASATINIB ANHYDROUS COMPOUND 11D [DOI: 10.1039/C0MD00194E]CENISERTIB PF-562271

Antibody

CAB070414 HPA071252

Interaction Protein

ENSG00000070831ENSG00000108953ENSG00000164924ENSG00000198752

Interaction Count

Interaction Dataset

intact_biogrid_opencellbiogrid_opencell