Protein detail

DCNL2

DCN1-like protein 2 (DCNL2) (DCUN1 domain-containing protein 2) (Defective in cullin neddylation protein 1-like protein 2)

Entry name DCNL2	UniProt ID Q6PH85	EVMP score 0.38
Frequency 3	Transmembrane count	Protein classification Predicted intracellular proteins

EVMP score: annotation confidence score.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information

Protein Names

DCN1-like protein 2 (DCNL2) (DCUN1 domain-containing protein 2) (Defective in cullin neddylation protein 1-like protein 2)

Protein Class

Predicted intracellular proteins

Protein Function

Predicted intracellular proteins

Ensembl

ENSG00000150401

Entrez Gene Symbol

DCUN1D2

Gene Synonym

C13orf17FLJ10704FLJ20092

Gene Description

Defective in cullin neddylation 1 domain containing 2

Chromosome

Position

113455819-113490951

Frequency

EVMP Score

0.38

Fluorescence & Localization

Tissue Specificlymphoid tissueCell SpecificInnate lymphoid cellsSingle-Nuclei Brain Specificcentral nervous system macrophageBlood Cell SpecificMAIT T-cellBlood Lineage Specificdendritic cells

Function & Pathway

Protein Function

Predicted intracellular proteins

Cellular Component

Molecular Function

Biological Process

Reactome

Canonical Pathways

M160 Pid avb3 integrin pathway

Mediation Categories

Metabolism mediation

Relations & Evidence

Enzyme-Mediated Modification

0 records.

Ligand-Receptor Signaling

4 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No

Regulatory Interaction Network

0 records.

Protein Complex Composition

1 record.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
	DCUN1D2 UBE2M	P61081 Q6PH85	4:4	PDB	PDB:4gao

Isolation & Detection Technology

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion Chromatography	Mass spectrometry [LTQ-FT Ultra]Small R sequencing (Illumi HiSeq 2000 (Solexa)Mass spectrometry	7	40189497 40730843 36146834 33309826 31805958 38207106 34576246

Sequence, Structure & Domains

Sequences

Length

259

Mass

30,179

Sequence

MHKLKSSQKDKVRQFMACTQAGERTAIYCLTQNEWRLDEATDSFFQNPDSLHRESMRNAVDKKKLERLYGRYKDPQDENKIGVDGIQQFCDDLSLDPASISVLVIAWKFRAATQCEFSRKEFLDGMTELGCDSMEKLKALLPRLEQELKDTAKFKDFYQFTFTFAKNPGQKGLDLEMAVAYWKLVLSGRFKFLDLWNTFLMEHHKRSIPRDTWNLLLDFGNMIADDMSNYDEEGAWPVLIDDFVEYARPVVTGGKRSLF

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q6PH85-1; Sequence=Displayed; Name=2; IsoId=Q6PH85-2; Sequence=VSP_015315, VSP_015316

Alternative Sequence

174..186; DLEMAVAYWKLVL -> GSPPFLNVKALHH (in isoform 2); 187..259; Missing (in isoform 2)

3D Structural Models

Turn

187..189

Helix

62..70; 83..92; 100..109; 119..128; 134..146; 147..149; 151..165; 175..185; 193..202; 210..222; 238..247; 249..251

Beta Strand

77..81; 171..174

3D Structure

X-ray crystallography (1)

Domain & Motif Annotations

Domain (CC)

The DCUN1 domain, also known as PONY domain, mediates the interaction with different cullins (PubMed:23201271). The DCUN1 domain mediates the interaction with the N-terminally acetylated NEDD8-conjugating E2s enzyme leading to the NEDD8 transfer from N-terminally acetylated NEDD8-conjugating E2s enzyme to different cullin C-terminal domain-RBX complexes; the neddylation efficiency correlates with the DCUN1D5-cullin and DCUN1D5-E2 interaction affinities (PubMed:23201271).

Domain (FT)

8..45; UBA-like; 60..248; DCUN1

Clinical Relevance

Antibody

HPA039349