Protein detail

DCNL2

DCN1-like protein 2 (DCNL2) (DCUN1 domain-containing protein 2) (Defective in cullin neddylation protein 1-like protein 2)

Entry name
DCNL2
UniProt ID
EVMP confidence score
0.38
Supporting publications (n)
3
Transmembrane count
Protein classification
Predicted intracellular proteins
EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40
Basic Information11
Protein Names
DCN1-like protein 2 (DCNL2) (DCUN1 domain-containing protein 2) (Defective in cullin neddylation protein 1-like protein 2)
Protein Class
Predicted intracellular proteins
Protein Function
Predicted intracellular proteins
Entrez Gene Symbol
Gene Synonym (3)
C13orf17FLJ10704FLJ20092
Gene Description
Defective in cullin neddylation 1 domain containing 2
Chromosome
13
Position
113455819-113490951
Supporting publications (n)
3
EVMP confidence score
0.38
Fluorescence & Localization5
Tissue Specificlymphoid tissueCell SpecificInnate lymphoid cellsSingle-Nuclei Brain Specificcentral nervous system macrophageBlood Cell SpecificMAIT T-cellBlood Lineage Specificdendritic cells
Function & Pathway7
Relations & Evidence6

Ligand-Receptor Signaling (4)

4 records.

CategoryParentDatabaseTransmitterReceiverSecretedPlasma Membrane (Transmembrane)Plasma Membrane (Peripheral)
intracellularintracellularComPPINoNoNoNoNo
intracellularintracellularGO_IntercellNoNoNoNoNo
intracellularintracellularUniProt_locationNoNoNoNoNo
intracellularintracellularOmniPathNoNoNoNoNo

Protein Complex Composition (1)

1 record.

Component NameComponent Gene SymbolsComponent UniProt IDStoichiometryDatabaseDatabase IDsReferences
DCUN1D2UBE2MP61081Q6PH854:4PDBPDB:4gao

Isolation & Detection Technology (1)

1 record.

EV Isolation MethodDetection MethodNumber of ReferencesReferences
Differential UltracentrifugationSize Exclusion ChromatographyMass spectrometry [LTQ-FT Ultra]Small R sequencing (Illumi HiSeq 2000 (Solexa)Mass spectrometry740189497407308433614683433309826318059583820710634576246
Sequence, Structure & Domains11

Sequences

Length
259
Mass
30,179
Sequence
MHKLKSSQKDKVRQFMACTQAGERTAIYCLTQNEWRLDEATDSFFQNPDSLHRESMRNAVDKKKLERLYGRYKDPQDENKIGVDGIQQFCDDLSLDPASISVLVIAWKFRAATQCEFSRKEFLDGMTELGCDSMEKLKALLPRLEQELKDTAKFKDFYQFTFTFAKNPGQKGLDLEMAVAYWKLVLSGRFKFLDLWNTFLMEHHKRSIPRDTWNLLLDFGNMIADDMSNYDEEGAWPVLIDDFVEYARPVVTGGKRSLF
Alternative Products
Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q6PH85-1; Sequence=Displayed; Name=2; IsoId=Q6PH85-2; Sequence=VSP_015315, VSP_015316
Alternative Sequence
174..186; DLEMAVAYWKLVL -> GSPPFLNVKALHH (in isoform 2); 187..259; Missing (in isoform 2)

3D Structural Models

Turn
187..189
Helix
62..70; 83..92; 100..109; 119..128; 134..146; 147..149; 151..165; 175..185; 193..202; 210..222; 238..247; 249..251
Beta Strand
77..81; 171..174
3D Structure
X-ray crystallography (1)

Domain & Motif Annotations

Domain (CC)
The DCUN1 domain, also known as PONY domain, mediates the interaction with different cullins (PubMed:23201271). The DCUN1 domain mediates the interaction with the N-terminally acetylated NEDD8-conjugating E2s enzyme leading to the NEDD8 transfer from N-terminally acetylated NEDD8-conjugating E2s enzyme to different cullin C-terminal domain-RBX complexes; the neddylation efficiency correlates with the DCUN1D5-cullin and DCUN1D5-E2 interaction affinities (PubMed:23201271).
Domain (FT)
8..45; UBA-like; 60..248; DCUN1
Clinical Relevance1
Antibody
Supporting Publications3
PMIDTitleAbstract
32795414Extracellular Vesicle and Particle Biomarkers Define Multiple Human Cancers.Among traditional exosome markers, CD9, HSPA8, ALIX, and HSP90AB1 represent pan-EVP markers, while ACTB, MSN, and RAP1B are novel pan-EVP markers.
38225453Deep proteomic analysis of obstetric antiphospholipid syndrome by DIA-MS of extracellular vesicle enriched fractions.No abstract available
38490958Proteomic analysis of ascitic extracellular vesicles describes tumour microenvironment and predicts patient survival in ovarian cancer.No abstract available