Protein detail

ATL4

ADAMTS-like protein 4 (ADAMTSL-4) (Thrombospondin repeat-containing protein 1)

Entry name ATL4	UniProt ID Q6UY14	EVMP confidence score 0.38
Supporting publications (n) 2	Transmembrane count	Protein classification Disease related genesHuman disease related genesPredicted secreted proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

ADAMTS-like protein 4 (ADAMTSL-4) (Thrombospondin repeat-containing protein 1)

Protein Class (3)

Disease related genesHuman disease related genesPredicted secreted proteins

Protein Function (3)

Disease related genes
Human disease related genes:Congenital malformations:Congenital malformations of eye
Predicted secreted proteins

Ensembl

ENSG00000143382

Entrez Gene Symbol

ADAMTSL4

Gene Synonym (2)

DKFZP434K1772TSRC1

Gene Description

ADAMTS like 4

Chromosome

Position

150549369-150560937

Supporting publications (n)

EVMP confidence score

0.38

Fluorescence & Localization5

Tissue SpecificintestineCell SpecificCytotrophoblastsSingle-Nuclei Brain Specificmammillary bodySecretome LocationSecreted in other tissuesSecretome FunctionReceptor

Function & Pathway6

Protein Function (3)

Disease related genes
Human disease related genes:Congenital malformations:Congenital malformations of eye
Predicted secreted proteins

Cellular Component (4)

Molecular Function (3)

Biological Process (3)

Reactome (6)

Mediation Categories (2)

Clinical-translation mediationMetabolism mediation

Relations & Evidence20

Ligand-Receptor Signaling (19)

19 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	GO_Intercell	No	No	Yes	No	No
intracellular	intracellular	OmniPath	No	No	Yes	No	No
ecm_regulator	ecm_regulator	Matrisome	Yes	No	Yes	No	No
ecm_regulator	ecm_regulator	OmniPath	Yes	No	Yes	No	No
secreted_peptidase	secreted_peptidase	OmniPath	No	No	Yes	No	No
secreted	secreted	UniProt_keyword	No	No	Yes	No	No
secreted	secreted	UniProt_location	No	No	Yes	No	No
secreted	secreted	HPA_secretome	No	No	Yes	No	No
secreted	secreted	OmniPath	No	No	Yes	No	No

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Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion Chromatography	Mass spectrometry	1	40689422

Sequence, Structure & Domains8

Sequences

Length

1,074

Mass

116,545

Sequence

MENWTGRPWLYLLLLLSLPQLCLDQEVLSGHSLQTPTEEGQGPEGVWGPWVQWASCSQPCGVGVQRRSRTCQLPTVQLHPSLPLPPRPPRHPEALLPRGQGPRPQTSPETLPLYRTQSRGRGGPLRGPASHLGREETQEIRAARRSRLRDPIKPGMFGYGRVPFALPLHRNRRHPRSPPRSELSLISSRGEEAIPSPTPRAEPFSANGSPQTELPPTELSVHTPSPQAEPLSPETAQTEVAPRTRPAPLRHHPRAQASGTEPPSPTHSLGEGGFFRASPQPRRPSSQGWASPQVAGRRPDPFPSVPRGRGQQGQGPWGTGGTPHGPRLEPDPQHPGAWLPLLSNGPHASSLWSLFAPSSPIPRCSGESEQLRACSQAPCPPEQPDPRALQCAAFNSQEFMGQLYQWEPFTEVQGSQRCELNCRPRGFRFYVRHTEKVQDGTLCQPGAPDICVAGRCLSPGCDGILGSGRRPDGCGVCGGDDSTCRLVSGNLTDRGGPLGYQKILWIPAGALRLQIAQLRPSSNYLALRGPGGRSIINGNWAVDPPGSYRAGGTVFRYNRPPREEGKGESLSAEGPTTQPVDVYMIFQEENPGVFYQYVISSPPPILENPTPEPPVPQLQPEILRVEPPLAPAPRPARTPGTLQRQVRIPQMPAPPHPRTPLGSPAAYWKRVGHSACSASCGKGVWRPIFLCISRESGEELDERSCAAGARPPASPEPCHGTPCPPYWEAGEWTSCSRSCGPGTQHRQLQCRQEFGGGGSSVPPERCGHLPRPNITQSCQLRLCGHWEVGSPWSQCSVRCGRGQRSRQVRCVGNNGDEVSEQECASGPPQPPSREACDMGPCTTAWFHSDWSSKCSAECGTGIQRRSVVCLGSGAALGPGQGEAGAGTGQSCPTGSRPPDMRACSLGPCERTWRWYTGPWGECSSECGSGTQRRDIICVSKLGTEFNVTSPSNCSHLPRPPALQPCQGQACQDRWFSTPWSPCSRSCQGGTQTREVQCLSTNQTLSTRCPPQLRPSRKRPCNSQPCSQRPDDQCKDSSPHCPLVVQARLCVYPYYTATCCRSCAHVLERSPQDPS

Alternative Products

Event=Alternative splicing; Named isoforms=3; Name=1; IsoId=Q6UY14-1; Sequence=Displayed; Name=2; IsoId=Q6UY14-2; Sequence=VSP_052183, VSP_052184; Name=3; IsoId=Q6UY14-3; Sequence=VSP_055759

Alternative Sequence

411; E -> EAPLLPLRHAFFLLPGAGSGDSTG (in isoform 3); 854..877; CSAECGTGIQRRSVVCLGSGAALG -> VSPEPPAISCILGNHAQDTSAFPA (in isoform 2); 878..1074; Missing (in isoform 2)

Domain & Motif Annotations

Compositional Bias

103..119; Polar residues; 132..152; Basic and acidic residues; 206..226; Polar residues; 310..323; Gly residues

Domain (FT)

48..93; TSP type-1 1; 723..782; TSP type-1 2; 783..842; TSP type-1 3; 845..909; TSP type-1 4; 910..969; TSP type-1 5; 970..1026; TSP type-1 6; 1029..1066; PLAC

Region

77..342; Disordered

Clinical Relevance1

Antibody

HPA006279

Supporting Publications2

PMID	Title	Abstract
36443833	UBE2O ubiquitinates PTRF/CAVIN1 and inhibits the secretion of exosome-related PTRF/CAVIN1.	And PTRF in exosomes has been identified as a potential biomarker in multiple malignancies such as glioma and renal cell carcinoma. Functionally, we found that UBE2O inhibited the effects of PTRF on exosome secretion via decreasing caveolae formation. Furthermore, we found that SDPR promotes PTRF expression in exosomes. However, the mechanisms of how to regulate the secretion of exosome-related PTRF remain unknown. Importantly, UBE2O decreased exosome secretion, resulting in downregulating PTRF secretion via exosomes. Interestingly, even in the presence of SDPR, UBE2O still inhibited the secretion of exosome-related PTRF. It is known that Polymerase I and Transcript Release Factor (PTRF, also known as Caveolin-associated Protein-1, CAVIN1) plays an important role in caveolae formation and exosome secretion. Our study demonstrated that UBE2O downregulated exosome release and controlled the secretion of exosome-related PTRF through ubiquitinating PTRF. Since exosomes play an important role in malignant tumor growth and PTRF included in exosomes is a biomarker for several malignant tumors, increasing UBE2O expression in cells has the potential to be developed as a novel approach for cancer treatment. The effect of UBE2O on the secretion of exosome-related PTRF was analyzed by western blot, and the effect of UBE2O on exosome secretion was evaluated by exosome markers and the total protein content of exosomes.
38385960	Urinary exosomal mRNA as a biomarker for the diagnosis of bladder cancer.	The combined detection of urinary exosome SDPR and ACBD7 has a certain diagnostic value for bladder cancer.