Protein detail

BAKOR

Beclin 1-associated autophagy-related key regulator (Barkor) (Autophagy-related protein 14-like protein) (Atg14L)

Entry name BAKOR	UniProt ID Q6ZNE5	EVMP confidence score 0.50
Supporting publications (n) 3	Transmembrane count	Protein classification Predicted intracellular proteinsTransporters

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

Beclin 1-associated autophagy-related key regulator (Barkor) (Autophagy-related protein 14-like protein) (Atg14L)

Protein Class (2)

Predicted intracellular proteinsTransporters

Protein Function (2)

Transporters
Predicted intracellular proteins

Ensembl

ENSG00000126775

Entrez Gene Symbol

ATG14

Gene Synonym (2)

ATG14LKIAA0831

Gene Description

Autophagy related 14

Chromosome

Position

55366391-55411830

Supporting publications (n)

EVMP confidence score

0.50

Fluorescence & Localization2

Tissue Specificbone marrowCell SpecificNeutrophil progenitors

Function & Pathway7

Protein Function (2)

Transporters
Predicted intracellular proteins

Cellular Component (12)

Page 1 of 2

Molecular Function (5)

Biological Process (3)

KEGG (8)

Reactome (13)

Page 1 of 2

Mediation Categories (5)

Clinical-translation mediationFusion and delivery mediationImmune mediationMetabolism mediationReceptor-signaling mediation

Relations & Evidence18

Enzyme-Mediated Modification (1)

1 record.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
ATG14	ULK1	O75385	S	29	phosphorylation	Sparser_ProtMapperProtMapperRLIMS-P_ProtMapperREACH_ProtMapperPhosphoSite	ProtMapper:27938392 ProtMapper:30397185 ProtMapper:29371398 ProtMapper:29313410

Ligand-Receptor Signaling (4)

4 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No

Regulatory Interaction Network (2)

2 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
ULK1	O75385	BAKOR	Q6ZNE5	Yes	Yes	No	Sparser_ProtMapperARNSIGNORProtMapperRLIMS-P_ProtMapperHINTREACH_ProtMapper	ProtMapper:27938392 HINT:27046250 ProtMapper:30397185 ProtMapper:29313410 SIGNOR:27938392 ProtMapper:31554253 ARN:23685627 ProtMapper:29371398 HINT:27938392
BAKOR	Q6ZNE5	PK3C3	Q8NEB9	Yes	Yes	No	InnateDBHINTSIGNORIntAct	HINT:23332761 InnateDB:19050071 HINT:35271311 IntAct:19050071 HINT:20562859 HINT:19050071 IntAct:22493499 SIGNOR:19270693 InnateDB:18843052 HINT:33961781 HINT:33422265 HINT:22493499 HINT:25490155 HINT:24785657 HINT:18843052 HINT:28757208

Protein Complex Composition (10)

10 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
HT_DM_Cluster294	ALDOA ALDOB ALDOC ATG14	P04075 P05062 P09972 Q6ZNE5	1:1:1:1	Compleat	Compleat:HC2013	22036573
ULK1-ATG13-ATG14-PIK3C3 complex	ATG13 ATG14 PIK3C3 ULK1	O75143 O75385 Q6ZNE5 Q8NEB9	0:0:0:0	CORUM	CORUM:6816	27046250
Vps34 Complex I	ATG14 BECN1 PIK3C3 PIK3R4	Q14457 Q6ZNE5 Q8NEB9 Q99570	1:1:1:1	SIGNORComplexPortalCompleatCFinderPDB	Compleat:HC7390SIGNOR:SIGNOR-C242intact:EBI-10687239PDB:8sor	14755292 16467569
	ATG14 BECN1 PIK3C3 PIK3R4 RAB1A	P62820 Q14457 Q6ZNE5 Q8NEB9 Q99570	1:1:1:1:1	PDB	PDB:9mhhPDB:9mhgPDB:9mhf
	ATG14 BECN1 MTCL3 NRBF2 PIK3C3 PIK3R4	Q14457 Q5TF21 Q6ZNE5 Q8NEB9 Q96F24 Q99570	0:0:0:0:0:0	hu.MAP2
	ATG14 BECN1 PIK3C3	Q14457 Q6ZNE5 Q8NEB9	0:0:0	KEGG-MEDICUS
	ATG14 BECN1 NRBF2 PIK3C3 PIK3R4	Q14457 Q6ZNE5 Q8NEB9 Q96F24 Q99570	0:0:0:0:0	KEGG-MEDICUShu.MAP2
	AMBRA1 ATG14 BECN1 PIK3C3 PIK3R4	Q14457 Q6ZNE5 Q8NEB9 Q99570 Q9C0C7	0:0:0:0:0	KEGG-MEDICUS
	ATG14 MTCL3 NRBF2	Q5TF21 Q6ZNE5 Q96F24	0:0:0	hu.MAP
	ATG14 GABARAPL1	Q6ZNE5 Q9H0R8	2:2	PDB	PDB:6hol

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationUltrafiltration / Tangential Flow FiltrationSize Exclusion ChromatographyImmunoaffinity Capture	Mass spectrometry	21	40985879 26826536 37926756 36064647 38113368 40465195 37322475 33304478 36497184 29891991 34064677 40189497 33592500 40940449 32916986 41307968 33309826 31805958 38607062 38207106 37670049

Sequence, Structure & Domains15

Sequences

Length

492

Mass

55,309

Sequence

MASPSGKGARALEAPGCGPRPLARDLVDSVDDAEGLYVAVERCPLCNTTRRRLTCAKCVQSGDFVYFDGRDRERFIDKKERLSRLKSKQEEFQKEVLKAMEGKWITDQLRWKIMSCKMRIEQLKQTICKGNEEMEKNSEGLLKTKEKNQKLYSRAQRHQEKKEKIQRHNRKLGDLVEKKTIDLRSHYERLANLRRSHILELTSVIFPIEEVKTGVRDPADVSSESDSAMTSSTVSKLAEARRTTYLSGRWVCDDHNGDTSISITGPWISLPNNGDYSAYYSWVEEKKTTQGPDMEQSNPAYTISAALCYATQLVNILSHILDVNLPKKLCNSEFCGENLSKQKFTRAVKKLNANILYLCFSQHVNLDQLQPLHTLRNLMYLVSPSSEHLGRSGPFEVRADLEESMEFVDPGVAGESDESGDERVSDEETDLGTDWENLPSPRFCDIPSQSVEVSQSQSTQASPPIASSSAGGMISSAAASVTSWFKAYTGHR

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q6ZNE5-1; Sequence=Displayed; Name=2; IsoId=Q6ZNE5-2; Sequence=VSP_013931

Alternative Sequence

1..113; Missing (in isoform 2)

3D Structural Models

Turn

387..390

Helix

57..61; 75..204; 277..280; 299..321; 331..334; 341..361; 366..368; 374..382; 399..405

Beta Strand

208..210; 260..265; 268..271; 335..337; 435..437

3D Structure

Electron microscopy (5); X-ray crystallography (1)

Domain & Motif Annotations

Compositional Bias

415..433; Acidic residues; 448..473; Low complexity

Coiled Coil

71..180

Domain (CC)

The coiled-coil domain is required for BECN1- and PIK3C3-binding and for autophagy.; DOMAIN: The final 80 residues in the C-terminus define a minimum required region for autophagosome binding called BATS.; DOMAIN: The N-terminal cysteine repeats are required for proper localization to the endoplasmic reticulum.

Region

43..58; Cysteine repeats; 410..473; Disordered; 413..492; BATS

Protein Families

ATG14 family

Sequence Similarities

Belongs to the ATG14 family.

Clinical Relevance4

Antibody (2)

CAB037242 CAB080659

Interaction Protein (5)

ENSG00000078142ENSG00000085449ENSG00000126581ENSG00000148572ENSG00000196455

Interaction Count

Interaction Dataset (3)

intact_biogrid_opencell_bioplexbiogrid_bioplexbiogrid_opencell_bioplex

Supporting Publications3

PMID	Title	Abstract
27894104	Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.	No abstract available
33709510	Unbiased proteomic profiling of host cell extracellular vesicle composition and dynamics upon HIV-1 infection.	No abstract available
37786918	Rapid and in-depth proteomic profiling of small extracellular vesicles for ultralow samples.	No abstract available