Protein detail

MARK2

Serine/threonine-protein kinase MARK2 (EC 2.7.11.1) (EC 2.7.11.26) (ELKL motif kinase 1) (EMK-1) (MAP/microtubule affinity-regulating kinase 2) (PAR1 homolog) (PAR1 homolog b) (Par-1b) (Par1b)

Protein symbol MARK2	UniProt ID Q7KZI7	EVMP score 0.50
Frequency 5	Transmembrane count	Protein classification EnzymesPredicted intracellular proteins

Basic Information

Protein Names

Serine/threonine-protein kinase MARK2 (EC 2.7.11.1) (EC 2.7.11.26) (ELKL motif kinase 1) (EMK-1) (MAP/microtubule affinity-regulating kinase 2) (PAR1 homolog) (PAR1 homolog b) (Par-1b) (Par1b)

Protein Class

EnzymesPredicted intracellular proteins

Protein Function

ENZYME proteins:Transferases
Enzymes
Predicted intracellular proteins
Kinases:CAMK Ser/Thr protein kinases

Ensembl

ENSG00000072518

Entrez Gene Symbol

MARK2

Gene Synonym

EMK1PAR-1PAR-1BPar1b

Gene Description

Microtubule affinity regulating kinase 2

Chromosome

Position

63838928-63911020

Frequency

EVMP Score

0.50

Fluorescence & Localization

Tissue Specificblood vesselCell SpecificAlveolar cells type 1Single-Nuclei Brain Specificendothelial cell

Function & Pathway

Protein Function

ENZYME proteins:Transferases
Enzymes
Predicted intracellular proteins
Kinases:CAMK Ser/Thr protein kinases

Cellular Component

Molecular Function

Biological Process

Mediation Categories

Receptor-signaling mediation

Relations & Evidence

Enzyme-Mediated Modification

20 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
MARK2	STK11	Q15831	T	208	phosphorylation	BEL-Large-Corpus_ProtMapperPhosphoNetworksphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperdbPTMKEAphosphoELMSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	dbPTM:17192257 phosphoELM:14976552 dbPTM:19369195 ProtMapper:14976552 dbPTM:14976552 KEA:17192257 KEA:14976552 SIGNOR:14976552
MARK2	STK11	Q15831	T	175	phosphorylation	KEA	KEA:14976552
MARK2	PRKCA	P17252	T	596	phosphorylation	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPKEAphosphoELM	KEA:15324659 phosphoELM:15324659
MARK2	PRKCA	P17252	S	409	phosphorylation	PhosphoNetworks
MARK2	PRKCA	P17252	T	603	phosphorylation	PhosphoNetworks
MARK2	PRKCA	P17252	T	508	phosphorylation	KEA	KEA:15324659
MARK2	PRKCA	P17252	T	562	phosphorylation	KEA	KEA:15324659
MARK2	PRKCA	P17252	T	563	phosphorylation	KEA	KEA:15324659
MARK2	TAOK1	Q7L7X3	T	208	phosphorylation	dbPTM	dbPTM:17192257 dbPTM:14976552 dbPTM:19369195
MARK2	PRKCZ	Q05513	T	596	phosphorylation	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperKEASIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	KEA:15324659 SIGNOR:15084291 ProtMapper:15084291

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Ligand-Receptor Signaling

12 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
plasma_membrane	plasma_membrane	OmniPath	No	No	No	No	No
receptor	receptor	scConnect	No	Yes	No	No	No

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Regulatory Interaction Network

24 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
MARK2	Q7KZI7	HDAC7	Q8WUI4	Yes	No	Yes	Sparser_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetSIGNORProtMapperSIGNOR_ProtMapperPhosphoSite_ProtMapper	SIGNOR:16980613 ProtMapper:32818796 ProtMapper:16980613
MARK2	Q7KZI7	KI13B	Q9NQT8	Yes	No	Yes	Sparser_ProtMapperPhosphoSite_norefSIGNORiPTMnetProtMapperdbPTMHINTSIGNOR_ProtMapperREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper	PhosphoSite:20194617 dbPTM:17525332 dbPTM:20194617 dbPTM:19690332 HINT:20194617 SIGNOR:20194617 ProtMapper:20194617 HINT:19615732 ProtMapper:24244223
TAOK1	Q7L7X3	MARK2	Q7KZI7	Yes	Yes	No	dbPTMiPTMnetSIGNOR	SIGNOR:18424437 dbPTM:14976552 dbPTM:17192257 dbPTM:19369195
KAPCA	P17612	MARK2	Q7KZI7	Yes	No	Yes	PhosphoSite_norefSIGNORPhosphoSite_ProtMapperProtMapper	SIGNOR:25512381
MARK2	Q7KZI7	RFIP1	Q6WKZ4	Yes	Yes	No	PhosphoSiteSIGNOR	SIGNOR:28396819 PhosphoSite:28396819
MARK2	Q7KZI7	UTRN	P46939	Yes	Yes	No	PhosphoSite_MIMPMIMPiPTMnetSIGNORProtMapperRLIMS-P_ProtMapperHINTSIGNOR_ProtMapperREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper	PhosphoSite:19945424 HINT:35271311 SIGNOR:19945424 HINT:19945424 ProtMapper:19945424 HINT:19615732
MARK2	Q7KZI7	PARD3	Q8TEW0	Yes	Yes	No	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPPhosphoSite_norefSIGNORiPTMnetProtMapperSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	SIGNOR:22883624 PhosphoSite:22883624 PhosphoSite:14653998 ProtMapper:22883624
KPCD	Q05655	MARK2	Q7KZI7	Yes	No	No	PhosphoSitePhosphoSite_ProtMapperProtMapper	PhosphoSite:15324659 PhosphoSite:33705388 PhosphoSite:19011111
MARK2	Q7KZI7	IF2A	P05198	Yes	Yes	No	Sparser_ProtMapperProtMapperWangPhosphoSitePhosphoSite_ProtMapper	PhosphoSite:16987104 PhosphoSite:17553788 PhosphoSite:25660019 PhosphoSite:25020061 PhosphoSite:25589675 PhosphoSite:24920670 PhosphoSite:34426706 PhosphoSite:11432733 ProtMapper:33705388 PhosphoSite:12483527 PhosphoSite:25843719 PhosphoSite:21151176 PhosphoSite:34244887 PhosphoSite:32054729 PhosphoSite:16952278 PhosphoSite:15060162 PhosphoSite:33400734 PhosphoSite:16179258 PhosphoSite:24092754 PhosphoSite:34615996 PhosphoSite:25759478 PhosphoSite:16179259 PhosphoSite:14676213 PhosphoSite:32205445 PhosphoSite:7622470 PhosphoSite:15684421 PhosphoSite:26100016 PhosphoSite:9930704 PhosphoSite:27259258 PhosphoSite:17522227 PhosphoSite:21577201 PhosphoSite:15314157 PhosphoSite:7641700 PhosphoSite:28683312 PhosphoSite:19109397 PhosphoSite:24942733 PhosphoSite:27375025 PhosphoSite:20385091 PhosphoSite:18562315
MARK2	Q7KZI7	HDAC4	P56524	Yes	No	No	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetProtMapperPhosphoSitePhosphoSite_ProtMapper	PhosphoSite:28319066 PhosphoSite:10869435 PhosphoSite:24140020 PhosphoSite:10958686 PhosphoSite:29472387 PhosphoSite:24904057

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Protein Complex Composition

13 records.

Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs
ARHGEF2 GAK MARK1 MARK2 MARK3 MARK4 PRKAA1 UBC USP21 YWHAB YWHAE YWHAG YWHAH YWHAQ YWHAZ	O14976 P0CG48 P27348 P27448 P31946 P61981 P62258 P63104 Q04917 Q13131 Q7KZI7 Q92974 Q96L34 Q9P0L2 Q9UK80	1:1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC9584
AKT1 MAP2K5 MAP3K5 MARK2 MARK3 PDPK1 PRKCI PRKCQ PRKCZ YWHAB YWHAE YWHAG YWHAH YWHAQ YWHAZ	O15530 P27348 P27448 P31749 P31946 P41743 P61981 P62258 P63104 Q04759 Q04917 Q05513 Q13163 Q7KZI7 Q99683	1:1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC7511
CAMK1D GMFG HDAC4 HDAC5 HDAC7 MARK1 MARK2 MARK3 SFN YWHAB YWHAE YWHAG YWHAH YWHAQ YWHAZ	O60234 P27348 P27448 P31946 P31947 P56524 P61981 P62258 P63104 Q04917 Q7KZI7 Q8IU85 Q8WUI4 Q9P0L2 Q9UQL6	1:1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC7982
FUCA1 MARK2	P04066 Q7KZI7	0:0	hu.MAP2
HSPA4 MARK1 MARK2 MARK3 MARK4 PMPCA PRKAA1 STK11 UBC USP21 YWHAB YWHAE YWHAG YWHAH YWHAZ	P0CG48 P27448 P31946 P34932 P61981 P62258 P63104 Q04917 Q10713 Q13131 Q15831 Q7KZI7 Q96L34 Q9P0L2 Q9UK80	1:1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC5977
ABHD17A ARHGEF2 HSPA13 MARK1 MARK2 MARK3 MARK4 PRKAA1 UBC USP21 YWHAB YWHAE YWHAG YWHAH YWHAZ	P0CG48 P27448 P31946 P48723 P61981 P62258 P63104 Q04917 Q13131 Q7KZI7 Q92974 Q96GS6 Q96L34 Q9P0L2 Q9UK80	1:1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC8431
CDK4 HCFC1 HCFC2 MARK1 MARK2 PPP1CC RIPK3	P11802 P36873 P51610 Q7KZI7 Q9P0L2 Q9Y572 Q9Y5Z7	1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC4817
EXO1 HDAC4 HDAC7 MARK1 MARK2 MARK3 PI4KB RALGPS2 SFN YWHAB YWHAE YWHAG YWHAH YWHAQ YWHAZ	P27348 P27448 P31946 P31947 P56524 P61981 P62258 P63104 Q04917 Q7KZI7 Q86X27 Q8WUI4 Q9P0L2 Q9UBF8 Q9UQ84	1:1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC6763
EXO1 HDAC7 MARK1 MARK2 MARK3 MARK4 PRKAA1 SFN USP21 YWHAB YWHAE YWHAG YWHAH YWHAQ YWHAZ	P27348 P27448 P31946 P31947 P61981 P62258 P63104 Q04917 Q13131 Q7KZI7 Q8WUI4 Q96L34 Q9P0L2 Q9UK80 Q9UQ84	1:1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC7270
ARHGEF2 HSPA4 MARK1 MARK2 MARK3 MARK4 PMPCA PRKAA1 WDR89 YWHAB YWHAE YWHAG YWHAH YWHAQ YWHAZ	P27348 P27448 P31946 P34932 P61981 P62258 P63104 Q04917 Q10713 Q13131 Q7KZI7 Q92974 Q96FK6 Q96L34 Q9P0L2	1:1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC7278

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Isolation & Detection Technology

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationUltrafiltration / Tangential Flow FiltrationSize Exclusion Chromatography	Mass spectrometry	9	28211531 33169421 31414377 29441425 25776846 28240915 32331267 33017416 34202855

Sequence, Structure & Domains

Sequences

Length

788

Mass

87,911

Sequence

MSSARTPLPTLNERDTEQPTLGHLDSKPSSKSNMIRGRNSATSADEQPHIGNYRLLKTIGKGNFAKVKLARHILTGKEVAVKIIDKTQLNSSSLQKLFREVRIMKVLNHPNIVKLFEVIETEKTLYLVMEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKFIVHRDLKAENLLLDADMNIKIADFGFSNEFTFGNKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKKFLILNPSKRGTLEQIMKDRWMNVGHEDDELKPYVEPLPDYKDPRRTELMVSMGYTREEIQDSLVGQRYNEVMATYLLLGYKSSELEGDTITLKPRPSADLTNSSAPSPSHKVQRSVSANPKQRRFSDQAAGPAIPTSNSYSKKTQSNNAENKRPEEDRESGRKASSTAKVPASPLPGLERKKTTPTPSTNSVLSTSTNRSRNSPLLERASLGQASIQNGKDSLTMPGSRASTASASAAVSAARPRQHQKSMSASVHPNKASGLPPTESNCEVPRPSTAPQRVPVASPSAHNISSSGGAPDRTNFPRGVSSRSTFHAGQLRQVRDQQNLPYGVTPASPSGHSQGRRGASGSIFSKFTSKFVRRNLSFRFARRNLNEPESKDRVETLRPHVVGSGGNDKEKEEFREAKPRSLRFTWSMKTTSSMEPNEMMREIRKVLDANSCQSELHEKYMLLCMHGTPGHEDFVQWEMEVCKLPRLSLNGVRFKRISGTSMAFKNIASKIANELKL

Alternative Products

Event=Alternative promoter usage, Alternative splicing; Named isoforms=16; Name=1; Synonyms=Alpha; IsoId=Q7KZI7-1; Sequence=Displayed; Name=2; IsoId=Q7KZI7-2; Sequence=VSP_051705, VSP_051706; Name=3; IsoId=Q7KZI7-3; Sequence=VSP_051705, VSP_051707; Name=4; Synonyms=Par-1Balpha; IsoId=Q7KZI7-4; Sequence=VSP_051706, VSP_051707; Name=5; IsoId=Q7KZI7-5; Sequence=VSP_051706, VSP_051708; Name=6; Synonyms=Beta; IsoId=Q7KZI7-6; Sequence=VSP_051705; Name=7; IsoId=Q7KZI7-7; Sequence=VSP_051705, VSP_051706, VSP_051707; Name=8; IsoId=Q7KZI7-8; Sequence=VSP_051707; Name=9; IsoId=Q7KZI7-9; Sequence=VSP_051706; Name=10; IsoId=Q7KZI7-10; Sequence=VSP_051705, VSP_051706, VSP_051708; Name=11; IsoId=Q7KZI7-11; Sequence=VSP_051708; Name=12; IsoId=Q7KZI7-12; Sequence=VSP_051705, VSP_051708; Name=13; IsoId=Q7KZI7-13; Sequence=VSP_051705, VSP_039872, VSP_051706, VSP_051707; Name=14; IsoId=Q7KZI7-14; Sequence=VSP_051705, VSP_039872, VSP_051707; Name=15; IsoId=Q7KZI7-15; Sequence=VSP_039872, VSP_051706, VSP_041853; Name=16; IsoId=Q7KZI7-16; Sequence=VSP_039872, VSP_051706, VSP_051707

Alternative Sequence

1..33; Missing (in isoform 2, isoform 3, isoform 6, isoform 7, isoform 10, isoform 12, isoform 13 and isoform 14); 412; Missing (in isoform 13, isoform 14, isoform 15 and isoform 16); 505..558; Missing (in isoform 2, isoform 4, isoform 5, isoform 7, isoform 9, isoform 10, isoform 13, isoform 15 and isoform 16); 644..652; Missing (in isoform 3, isoform 4, isoform 7, isoform 8, isoform 13, isoform 14 and isoform 16); 645..668; Missing (in isoform 15); 654..668; Missing (in isoform 5, isoform 11, isoform 10 and isoform 12)

3D Structural Models

Turn

73..75

Helix

86..88; 93..106; 137..144; 149..168; 178..180; 213..215; 218..222; 230..245; 255..264; 275..284; 289..291; 295..298; 303..305; 326..335; 339..347; 353..360

Beta Strand

53..60; 63..72; 78..85; 115..120; 122..130; 181..183; 189..191; 309..311

3D Structure

X-ray crystallography (5)

Domain & Motif Annotations

Compositional Bias

27..45; Polar residues; 418..432; Polar residues; 433..445; Basic and acidic residues; 467..486; Polar residues; 495..504; Polar residues; 511..525; Low complexity

Domain (CC)

The UBA domain does not seem to bind ubiquitin and ubiquitin-like and might play a role in regulating the enzyme conformation and localization. Activation of the kinase activity following phosphorylation at Thr-208 is accompanied by a conformational change that alters the orientation of the UBA domain with respect to the catalytic domain.; DOMAIN: The KA1 domain mediates binding to phospholipids and targeting to membranes.

Domain (FT)

53..304; Protein kinase; 323..362; UBA; 739..788; KA1

Region

1..46; Disordered; 373..632; Disordered

Protein Families

Protein kinase superfamily
CAMK Ser/Thr protein kinase family
SNF1 subfamily

Sequence Similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.

Clinical Relevance

Antibody

HPA038790 HPA074905

Interaction Protein

ENSG00000108953ENSG00000128245ENSG00000134308ENSG00000152818ENSG00000164924ENSG00000166913ENSG00000168502ENSG00000170027

Interaction Count

Interaction Dataset

biogrid_opencellintact_biogrid_opencellintact_biogrid