Protein detail

MARK2

Serine/threonine-protein kinase MARK2 (EC 2.7.11.1) (EC 2.7.11.26) (ELKL motif kinase 1) (EMK-1) (MAP/microtubule affinity-regulating kinase 2) (PAR1 homolog) (PAR1 homolog b) (Par-1b) (Par1b)

Protein symbol MARK2	UniProt ID Q7KZI7	EVMP score 0.50
Frequency 5	Transmembrane count	Protein classification EnzymesPredicted intracellular proteins

Basic Information

Protein Names

Serine/threonine-protein kinase MARK2 (EC 2.7.11.1) (EC 2.7.11.26) (ELKL motif kinase 1) (EMK-1) (MAP/microtubule affinity-regulating kinase 2) (PAR1 homolog) (PAR1 homolog b) (Par-1b) (Par1b)

Protein Class

EnzymesPredicted intracellular proteins

Protein Function

ENZYME proteins:Transferases
Enzymes
Predicted intracellular proteins
Kinases:CAMK Ser/Thr protein kinases

Ensembl

ENSG00000072518

Entrez Gene Symbol

MARK2

Gene Synonym

EMK1PAR-1PAR-1BPar1b

Gene Description

Microtubule affinity regulating kinase 2

Chromosome

Position

63838928-63911020

Frequency

EVMP Score

0.50

Fluorescence & Localization

Tissue Specificblood vesselCell SpecificAlveolar cells type 1Single-Nuclei Brain Specificendothelial cell

Function & Pathway

Protein Function

ENZYME proteins:Transferases
Enzymes
Predicted intracellular proteins
Kinases:CAMK Ser/Thr protein kinases

Cellular Component

Molecular Function

Biological Process

Mediation Categories

Receptor-signaling mediation

Relations & Evidence

Enzyme-Mediated Modification

20 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
MARK2	PRKCD	Q05655	T	596	phosphorylation	PhosphoSite
MARK2	PRKACA	P17612	S	409	phosphorylation	PhosphoSite
MARK2	PRKCI	P41743	T	596	phosphorylation	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPProtMapperPhosphoSitePhosphoSite_ProtMapper
MARK2	PRKD1	Q15139	S	400	phosphorylation	Sparser_ProtMapperPhosphoSite_MIMPMIMPProtMapperRLIMS-P_ProtMapperREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper	ProtMapper:19011111 ProtMapper:26848698
MARK2	GSK3B	P49841	S	212	phosphorylation	Sparser_ProtMapperPhosphoSite_MIMPMIMPHPRD_MIMPProtMapperRLIMS-P_ProtMapperREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper	ProtMapper:16257959 ProtMapper:28236972
MARK2	PRKD2	Q9BZL6	S	400	phosphorylation	PhosphoSite_MIMPMIMPProtMapperPhosphoSitePhosphoSite_ProtMapper
MARK2	PKD1	P98161	S	400	phosphorylation	Sparser_ProtMapperProtMapper	ProtMapper:26848698
MARK2	MAPK10	P53779	S	483	phosphorylation	KEA	KEA:17570479
MARK2	MAPK9	P45984	S	422	phosphorylation	KEA	KEA:17570479
MARK2	PPP2CB	P62714	T	596	phosphorylation	KEA	KEA:15324659

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Ligand-Receptor Signaling

12 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
receptor	receptor	OmniPath	No	Yes	No	No	No
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
transmembrane	transmembrane	LOCATE	No	No	No	No	No
transmembrane	transmembrane	OmniPath	No	No	No	No	No
plasma_membrane	plasma_membrane	UniProt_location	No	No	No	No	No
lateral_cell_membrane	plasma_membrane	UniProt_location	No	No	No	No	No

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Regulatory Interaction Network

24 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
MARK2	Q7KZI7	TAU	P10636	Yes	No	Yes	WangSparser_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPNCI-PID_ProtMapperHPRD_MIMPiPTMnetSIGNORProtMapperREACH_ProtMapperBioGRIDIntActSPIKE_LCLit-BM-17SPIKE	Lit-BM-17:23666762 IntAct:23001711 ProtMapper:28066266 SIGNOR:10090741 ProtMapper:22952452 Lit-BM-17:23001711 ProtMapper:17573348 BioGRID:23001711 ProtMapper:7706316 ProtMapper:24860424 IntAct:23666762 SPIKE:9108484 ProtMapper:21629754 ProtMapper:30287790 ProtMapper:20067632 SPIKE_LC:9108484 Lit-BM-17:24251416
GSK3B	P49841	MARK2	Q7KZI7	Yes	Yes	Yes	Sparser_ProtMapperPhosphoSite_MIMPMIMPHPRD_MIMPPhosphoSite_norefSIGNORiPTMnetProtMapperHPRDRLIMS-P_ProtMapperSignaLink3REACH_ProtMapperPhosphoSite_ProtMapper	SignaLink3:16257959 SIGNOR:18424437 SIGNOR:16257959 ProtMapper:32471307 ProtMapper:28236972 ProtMapper:16257959 SignaLink3:23331499 HPRD:16257959
STK11	Q15831	MARK2	Q7KZI7	Yes	Yes	No	HPRD_MIMPSIGNORProtMapperdbPTMPhosphoSite_KEASignaLink3phosphoELM_KEAPhosphoNetworksHPRDKinexus_KEAWangPhosphoSite_ProtMapperBEL-Large-Corpus_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPiPTMnetPhosphoPointKEAphosphoELMSIGNOR_ProtMapperREACH_ProtMapperPhosphoSite	dbPTM:17192257 phosphoELM:14976552 PhosphoSite:17573348 PhosphoSite:28711359 SignaLink3:14976552 SIGNOR:18424437 SignaLink3:17573348 dbPTM:19369195 ProtMapper:32841502 ProtMapper:14976552 HPRD:14976552 dbPTM:14976552 SignaLink3:23331499 KEA:17192257 KEA:14976552 PhosphoSite:22238344 SIGNOR:17573348 SIGNOR:14976552
MARK2	Q7KZI7	RFIP2	Q7L804	Yes	Yes	No	Sparser_ProtMapperPhosphoSite_MIMPMIMPiPTMnetSIGNORProtMapperdbPTMSIGNOR_ProtMapperREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper	ProtMapper:22553350 dbPTM:16775013 PhosphoSite:16775013 PhosphoSite:22553350 ProtMapper:26792722 ProtMapper:21143914 SIGNOR:16775013 ProtMapper:16775013
MARK2	Q7KZI7	PINK1	Q9BXM7	Yes	Yes	No	PhosphoSite_norefSIGNORiPTMnetProtMapperREACH_ProtMapperLit-BM-17PhosphoSite_ProtMapper	Lit-BM-17:22238344 ProtMapper:29255601 SIGNOR:22238344
MARK2	Q7KZI7	KSR1	Q8IVT5	Yes	No	Yes	REACH_ProtMapperSIGNORSparser_ProtMapperProtMapper	ProtMapper:22206009 ProtMapper:31481497 SIGNOR:22206009
MARK2	Q7KZI7	BAIP2	Q9UQB8	Yes	No	Yes	Sparser_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetSIGNORProtMapperREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper	SIGNOR:21282462 PhosphoSite:21282462 ProtMapper:21282462
MARK2	Q7KZI7	TNK1	Q13470	Yes	No	Yes	SIGNOR	SIGNOR:34504101
KPCZ	Q05513	MARK2	Q7KZI7	Yes	No	Yes	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetSIGNORProtMapperKEAKinexus_KEASIGNOR_ProtMapperPhosphoSite_ProtMapper	KEA:15324659 SIGNOR:15084291 ProtMapper:15084291
MARK2	Q7KZI7	ARHG2	Q92974	Yes	No	Yes	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPPhosphoSite_norefSIGNORiPTMnetProtMapperRLIMS-P_ProtMapperHINTSIGNOR_ProtMapperREACH_ProtMapperPhosphoSite_ProtMapper	HINT:19615732 HINT:21513698 SIGNOR:22072711 ProtMapper:22072711

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Protein Complex Composition

13 records.

Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs
ARHGEF2 GAK MARK1 MARK2 MARK3 MARK4 PRKAA1 UBC USP21 YWHAB YWHAE YWHAG YWHAH YWHAQ YWHAZ	O14976 P0CG48 P27348 P27448 P31946 P61981 P62258 P63104 Q04917 Q13131 Q7KZI7 Q92974 Q96L34 Q9P0L2 Q9UK80	1:1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC9584
AKT1 MAP2K5 MAP3K5 MARK2 MARK3 PDPK1 PRKCI PRKCQ PRKCZ YWHAB YWHAE YWHAG YWHAH YWHAQ YWHAZ	O15530 P27348 P27448 P31749 P31946 P41743 P61981 P62258 P63104 Q04759 Q04917 Q05513 Q13163 Q7KZI7 Q99683	1:1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC7511
CAMK1D GMFG HDAC4 HDAC5 HDAC7 MARK1 MARK2 MARK3 SFN YWHAB YWHAE YWHAG YWHAH YWHAQ YWHAZ	O60234 P27348 P27448 P31946 P31947 P56524 P61981 P62258 P63104 Q04917 Q7KZI7 Q8IU85 Q8WUI4 Q9P0L2 Q9UQL6	1:1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC7982
FUCA1 MARK2	P04066 Q7KZI7	0:0	hu.MAP2
HSPA4 MARK1 MARK2 MARK3 MARK4 PMPCA PRKAA1 STK11 UBC USP21 YWHAB YWHAE YWHAG YWHAH YWHAZ	P0CG48 P27448 P31946 P34932 P61981 P62258 P63104 Q04917 Q10713 Q13131 Q15831 Q7KZI7 Q96L34 Q9P0L2 Q9UK80	1:1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC5977
ABHD17A ARHGEF2 HSPA13 MARK1 MARK2 MARK3 MARK4 PRKAA1 UBC USP21 YWHAB YWHAE YWHAG YWHAH YWHAZ	P0CG48 P27448 P31946 P48723 P61981 P62258 P63104 Q04917 Q13131 Q7KZI7 Q92974 Q96GS6 Q96L34 Q9P0L2 Q9UK80	1:1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC8431
CDK4 HCFC1 HCFC2 MARK1 MARK2 PPP1CC RIPK3	P11802 P36873 P51610 Q7KZI7 Q9P0L2 Q9Y572 Q9Y5Z7	1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC4817
EXO1 HDAC4 HDAC7 MARK1 MARK2 MARK3 PI4KB RALGPS2 SFN YWHAB YWHAE YWHAG YWHAH YWHAQ YWHAZ	P27348 P27448 P31946 P31947 P56524 P61981 P62258 P63104 Q04917 Q7KZI7 Q86X27 Q8WUI4 Q9P0L2 Q9UBF8 Q9UQ84	1:1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC6763
EXO1 HDAC7 MARK1 MARK2 MARK3 MARK4 PRKAA1 SFN USP21 YWHAB YWHAE YWHAG YWHAH YWHAQ YWHAZ	P27348 P27448 P31946 P31947 P61981 P62258 P63104 Q04917 Q13131 Q7KZI7 Q8WUI4 Q96L34 Q9P0L2 Q9UK80 Q9UQ84	1:1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC7270
ARHGEF2 HSPA4 MARK1 MARK2 MARK3 MARK4 PMPCA PRKAA1 WDR89 YWHAB YWHAE YWHAG YWHAH YWHAQ YWHAZ	P27348 P27448 P31946 P34932 P61981 P62258 P63104 Q04917 Q10713 Q13131 Q7KZI7 Q92974 Q96FK6 Q96L34 Q9P0L2	1:1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC7278

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Isolation & Detection Technology

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationUltrafiltration / Tangential Flow FiltrationSize Exclusion Chromatography	Mass spectrometry	9	28211531 33169421 31414377 29441425 25776846 28240915 32331267 33017416 34202855

Sequence, Structure & Domains

Sequences

Length

788

Mass

87,911

Sequence

MSSARTPLPTLNERDTEQPTLGHLDSKPSSKSNMIRGRNSATSADEQPHIGNYRLLKTIGKGNFAKVKLARHILTGKEVAVKIIDKTQLNSSSLQKLFREVRIMKVLNHPNIVKLFEVIETEKTLYLVMEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKFIVHRDLKAENLLLDADMNIKIADFGFSNEFTFGNKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKKFLILNPSKRGTLEQIMKDRWMNVGHEDDELKPYVEPLPDYKDPRRTELMVSMGYTREEIQDSLVGQRYNEVMATYLLLGYKSSELEGDTITLKPRPSADLTNSSAPSPSHKVQRSVSANPKQRRFSDQAAGPAIPTSNSYSKKTQSNNAENKRPEEDRESGRKASSTAKVPASPLPGLERKKTTPTPSTNSVLSTSTNRSRNSPLLERASLGQASIQNGKDSLTMPGSRASTASASAAVSAARPRQHQKSMSASVHPNKASGLPPTESNCEVPRPSTAPQRVPVASPSAHNISSSGGAPDRTNFPRGVSSRSTFHAGQLRQVRDQQNLPYGVTPASPSGHSQGRRGASGSIFSKFTSKFVRRNLSFRFARRNLNEPESKDRVETLRPHVVGSGGNDKEKEEFREAKPRSLRFTWSMKTTSSMEPNEMMREIRKVLDANSCQSELHEKYMLLCMHGTPGHEDFVQWEMEVCKLPRLSLNGVRFKRISGTSMAFKNIASKIANELKL

Alternative Products

Event=Alternative promoter usage, Alternative splicing; Named isoforms=16; Name=1; Synonyms=Alpha; IsoId=Q7KZI7-1; Sequence=Displayed; Name=2; IsoId=Q7KZI7-2; Sequence=VSP_051705, VSP_051706; Name=3; IsoId=Q7KZI7-3; Sequence=VSP_051705, VSP_051707; Name=4; Synonyms=Par-1Balpha; IsoId=Q7KZI7-4; Sequence=VSP_051706, VSP_051707; Name=5; IsoId=Q7KZI7-5; Sequence=VSP_051706, VSP_051708; Name=6; Synonyms=Beta; IsoId=Q7KZI7-6; Sequence=VSP_051705; Name=7; IsoId=Q7KZI7-7; Sequence=VSP_051705, VSP_051706, VSP_051707; Name=8; IsoId=Q7KZI7-8; Sequence=VSP_051707; Name=9; IsoId=Q7KZI7-9; Sequence=VSP_051706; Name=10; IsoId=Q7KZI7-10; Sequence=VSP_051705, VSP_051706, VSP_051708; Name=11; IsoId=Q7KZI7-11; Sequence=VSP_051708; Name=12; IsoId=Q7KZI7-12; Sequence=VSP_051705, VSP_051708; Name=13; IsoId=Q7KZI7-13; Sequence=VSP_051705, VSP_039872, VSP_051706, VSP_051707; Name=14; IsoId=Q7KZI7-14; Sequence=VSP_051705, VSP_039872, VSP_051707; Name=15; IsoId=Q7KZI7-15; Sequence=VSP_039872, VSP_051706, VSP_041853; Name=16; IsoId=Q7KZI7-16; Sequence=VSP_039872, VSP_051706, VSP_051707

Alternative Sequence

1..33; Missing (in isoform 2, isoform 3, isoform 6, isoform 7, isoform 10, isoform 12, isoform 13 and isoform 14); 412; Missing (in isoform 13, isoform 14, isoform 15 and isoform 16); 505..558; Missing (in isoform 2, isoform 4, isoform 5, isoform 7, isoform 9, isoform 10, isoform 13, isoform 15 and isoform 16); 644..652; Missing (in isoform 3, isoform 4, isoform 7, isoform 8, isoform 13, isoform 14 and isoform 16); 645..668; Missing (in isoform 15); 654..668; Missing (in isoform 5, isoform 11, isoform 10 and isoform 12)

3D Structural Models

Turn

73..75

Helix

86..88; 93..106; 137..144; 149..168; 178..180; 213..215; 218..222; 230..245; 255..264; 275..284; 289..291; 295..298; 303..305; 326..335; 339..347; 353..360

Beta Strand

53..60; 63..72; 78..85; 115..120; 122..130; 181..183; 189..191; 309..311

3D Structure

X-ray crystallography (5)

Domain & Motif Annotations

Compositional Bias

27..45; Polar residues; 418..432; Polar residues; 433..445; Basic and acidic residues; 467..486; Polar residues; 495..504; Polar residues; 511..525; Low complexity

Domain (CC)

The UBA domain does not seem to bind ubiquitin and ubiquitin-like and might play a role in regulating the enzyme conformation and localization. Activation of the kinase activity following phosphorylation at Thr-208 is accompanied by a conformational change that alters the orientation of the UBA domain with respect to the catalytic domain.; DOMAIN: The KA1 domain mediates binding to phospholipids and targeting to membranes.

Domain (FT)

53..304; Protein kinase; 323..362; UBA; 739..788; KA1

Region

1..46; Disordered; 373..632; Disordered

Protein Families

Protein kinase superfamily
CAMK Ser/Thr protein kinase family
SNF1 subfamily

Sequence Similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.

Clinical Relevance

Antibody

HPA038790 HPA074905

Interaction Protein

ENSG00000108953ENSG00000128245ENSG00000134308ENSG00000152818ENSG00000164924ENSG00000166913ENSG00000168502ENSG00000170027

Interaction Count

Interaction Dataset

biogrid_opencellintact_biogrid_opencellintact_biogrid