Protein detail

PHLB2

Pleckstrin homology-like domain family B member 2 (Protein LL5-beta)

Entry name
PHLB2
UniProt ID
EVMP confidence score
0.63
Supporting publications (n)
6
Transmembrane count
Protein classification
Predicted intracellular proteinsPredicted membrane proteins
EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40
Basic Information11
Protein Names
Pleckstrin homology-like domain family B member 2 (Protein LL5-beta)
Protein Class (2)
Predicted intracellular proteinsPredicted membrane proteins
Protein Function
Predicted intracellular proteins
Entrez Gene Symbol
Gene Synonym (3)
FLJ21791LL5bLL5beta
Gene Description
Pleckstrin homology like domain family B member 2
Chromosome
3
Position
111732497-111976517
Supporting publications (n)
6
EVMP confidence score
0.63
Fluorescence & Localization4
Tissue SpecificbrainCell SpecificBergmann gliaBlood Cell SpecificneutrophilBlood Lineage Specificgranulocytes
Function & Pathway5
Relations & Evidence8

Enzyme-Mediated Modification (1)

1 record.

Substrate Gene SymbolEnzyme Gene SymbolEnzyme UniProt IDResidue TypeResidue OffsetModificationDatabaseReferences
PHLDB2CSNK2A2P19784S489phosphorylationKEAKEA:17570479

Ligand-Receptor Signaling (4)

4 records.

CategoryParentDatabaseTransmitterReceiverSecretedPlasma Membrane (Transmembrane)Plasma Membrane (Peripheral)
intracellularintracellularComPPINoNoNoNoNo
intracellularintracellularGO_IntercellNoNoNoNoNo
intracellularintracellularUniProt_locationNoNoNoNoNo
intracellularintracellularOmniPathNoNoNoNoNo

Protein Complex Composition (2)

2 records.

Component NameComponent Gene SymbolsComponent UniProt IDStoichiometryDatabaseDatabase IDsReferences
CLASP2PHLDB2O75122Q86SQ01:1PDBPDB:8whk
ACTBACTR3CAPZA1CAPZBHNRNPLLMYO6PHLDB2P47756P52907P60709P61158Q86SQ0Q8WVV9Q9UM540:0:0:0:0:0:0hu.MAP

Isolation & Detection Technology (1)

1 record.

EV Isolation MethodDetection MethodNumber of ReferencesReferences
Differential UltracentrifugationDensity Gradient CentrifugationUltrafiltration / Tangential Flow FiltrationSize Exclusion ChromatographyMass spectrometryWestern blottingMORPH23303581434817906
Sequence, Structure & Domains11

Sequences

Length
1,253
Mass
142,158
Sequence
MEEHSYIQKELDLQNGSLEEDSVVHSVENDSQNMMESLSPKKYSSSLRFKANGDYSGSYLTLSQPVPAKRSPSPLGTSVRSSPSLAKIQGSKQFSYDGTDKNIPMKPPTPLLNTTSSLSGYPLGRADFDHYTGRDSERALRLSEKPPYSKYSSRHKSHDNVYSLGGLEGRKASGSLLAMWNGSSLSDAGPPPISRSGAASMPSSPKQARKMSIQDSLALQPKLTRHKELASENINLRTRKYSSSSLSHMGAYSRSLPRLYRATENQLTPLSLPPRNSLGNSKRTKLGEKDLPHSVIDNDNYLNFSSLSSGALPYKTSASEGNPYVSSTLSVPASPRVARKMLLASTSSCASDDFDQASYVGTNPSHSLLAGESDRVFATRRNFSCGSVEFDEADLESLRQASGTPQPALRERKSSISSISGRDDLMDYHRRQREERLREQEMERLERQRLETILSLCAEYTKPDSRLSTGTTVEDVQKINKELEKLQLSDEESVFEEALMSPDTRYRCHRKDSLPDADLASCGSLSQSSASFFTPRSTRNDELLSDLTRTPPPPSSTFPKASSESSYLSILPKTPEGISEEQRSQELAAMEETRIVILNNLEELKQKIKDINDQMDESFRELDMECALLDGEQKSETTELMKEKEILDHLNRKIAELEKNIVGEKTKEKVKLDAEREKLERLQELYSEQKTQLDNCPESMREQLQQQLKRDADLLDVESKHFEDLEFQQLEHESRLDEEKENLTQQLLREVAEYQRNIVSRKEKISALKKQANHIVQQAQREQDHFVKEKNNLIMMLQREKENLCNLEKKYSSLSGGKGFPVNPNTLKEGYISVNEINEPCGNSTNLSPSTQFPADADAVATEPATAVLASQPQSKEHFRSLEERKKQHKEGLYLSDTLPRKKTTSSISPHFSSATMGRSITPKAHLPLGQSNSCGSVLPPSLAAMAKDSESRRMLRGYNHQQMSEGHRQKSEFYNRTASESNVYLNSFHYPDHSYKDQAFDTLSLDSSDSMETSISACSPDNISSASTSNIARIEEMERLLKQAHAEKTRLLESREREMEAKKRALEEEKRRREILEKRLQEETSQRQKLIEKEVKIRERQRAQARPLTRYLPVRKEDFDLRSHVETAGHNIDTCYHVSITEKTCRGFLIKMGGKIKTWKKRWFVFDRNKRTFSYYADKHETKLKGVIYFQAIEEVYYDHLKNANKSPNPLLTFSVKTHDRIYYMVAPSPEAMRIWMDVIVTGAEGYTHFLL
Alternative Products
Event=Alternative splicing; Named isoforms=3; Name=1; IsoId=Q86SQ0-1; Sequence=Displayed; Name=2; IsoId=Q86SQ0-2; Sequence=VSP_016745; Name=3; IsoId=Q86SQ0-3; Sequence=VSP_016744, VSP_016745
Alternative Sequence
1; M -> MEEEDTKREVPKEDGVGDVQHFDSSKIM (in isoform 3); 668..710; Missing (in isoform 2 and isoform 3)

3D Structural Models

3D Structure
X-ray crystallography (3)

Domain & Motif Annotations

Compositional Bias
1..12; Basic and acidic residues; 29..43; Polar residues; 74..96; Polar residues; 126..144; Basic and acidic residues
Coiled Coil
584..696; 722..807; 1032..1098
Domain (CC)
The PH domain mediates the binding to phosphoinositides.
Domain (FT)
1143..1246; PH
Region
1..43; Disordered; 60..159; Disordered; 187..212; Disordered; 265..286; Disordered; 525..567; Disordered
Clinical Relevance4
Interaction Protein (5)
ENSG00000108953ENSG00000164924ENSG00000166913ENSG00000170027ENSG00000187555
Interaction Count
5
Interaction Dataset (2)
biogrid_opencellintact_biogrid
Supporting Publications6
PMIDTitleAbstract
33613873Proteomic profile of melanoma cell-derived small extracellular vesicles in patients' plasma: a potential correlate of melanoma progression.No abstract available
34265469Proteomic Landscape of Exosomes Reveals the Functional Contributions of CD151 in Triple-Negative Breast Cancer.Furthermore, utilizing quantitative proteomics approach to reveal the proteomes of CD151-deleted exosomes and cells, we found that exosomal CD151 facilitated secretion of ribosomal proteins via exosomes while inhibiting exosome secretion of complement proteins. Moreover, we proved that CD151-deleted exosomes significantly decreased the migration and invasion of TNBC cells. Most importantly, we found that the tetraspanin CD151 expression levels in TNBC-derived serum exosomes were significantly higher than those exosomes from healthy subjects, and we validated our findings with samples from 16 additional donors. This is the first comparative study of the proteomes of TNBC patient-derived and CD151-deleted exosomes.
35741093Proteomic and Metabolomic Profiles of T Cell-Derived Exosomes Isolated from Human Plasma.No abstract available
36573687Proteomic and phosphoproteomic landscape of salivary extracellular vesicles to assess OSCC therapeutical outcomes.No abstract available
39408670Proteomic Characterization of Corneal Epithelial and Stromal Cell-Derived Extracellular Vesicles.No abstract available
39948040Quantitative proteomics identifies possible flow of metastatic cues between progressive stages of colorectal cancer via transfer of ceramide-dependent exosomal cargoes.No abstract available