Protein detail

RSPH3

Radial spoke head protein 3 homolog (A-kinase anchor protein RSPH3) (Radial spoke head-like protein 2)

Entry name RSPH3	UniProt ID Q86UC2	EVMP confidence score 0.38
Supporting publications (n) 2	Transmembrane count	Protein classification Human disease related genesPredicted intracellular proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

Radial spoke head protein 3 homolog (A-kinase anchor protein RSPH3) (Radial spoke head-like protein 2)

Protein Class (2)

Human disease related genesPredicted intracellular proteins

Protein Function (2)

Human disease related genes:Respiratory diseases:Tracheobronchial diseases
Predicted intracellular proteins

Ensembl

ENSG00000130363

Entrez Gene Symbol

RSPH3

Gene Synonym (3)

dJ111C20.1RSHL2RSP3

Gene Description

Radial spoke head 3

Chromosome

Position

158972871-159000202

Supporting publications (n)

EVMP confidence score

0.38

Fluorescence & Localization3

Tissue Specificlymphoid tissueCell SpecificB-cells

Function & Pathway5

Protein Function (2)

Human disease related genes:Respiratory diseases:Tracheobronchial diseases
Predicted intracellular proteins

Cellular Component (3)

Molecular Function

GO:0005515 protein binding

Biological Process (3)

Mediation Categories

Other mediation

Relations & Evidence12

Enzyme-Mediated Modification (3)

3 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
RSPH3	MAPK1	P28482	T	286	phosphorylation	SIGNOR	SIGNOR:19684019
RSPH3	MAPK1	P28482	T	243	phosphorylation	SIGNOR	SIGNOR:19684019
RSPH3	MAPK3	P27361	T	286	phosphorylation	PhosphoSitePhosphoSite_ProtMapperProtMapper

Ligand-Receptor Signaling (4)

4 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No

Regulatory Interaction Network (2)

2 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
MK01	P28482	RSPH3	Q86UC2	Yes	Yes	No	SIGNOR_ProtMapperiPTMnetSIGNORProtMapper	SIGNOR:19684019 ProtMapper:19684019
MK03	P27361	RSPH3	Q86UC2	Yes	No	No	iPTMnetPhosphoSitePhosphoSite_ProtMapperProtMapper	PhosphoSite:19684019

Protein Complex Composition (2)

2 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
	ATXN7L3 ENY2 KAT2A RSPH3 UBC USP22	P0CG48 Q14CW9 Q86UC2 Q92830 Q9NPA8 Q9UPT9	1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC5499
	ROPN1L RSPH14 RSPH3	Q86UC2 Q96C74 Q9UHP6	0:0:0	hu.MAP2

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion Chromatography	Mass spectrometry	1	38716512

Sequence, Structure & Domains11

Sequences

Length

560

Mass

63,687

Sequence

MTVKPAKAASLARNLAKRRRTYLGGAAGRSQEPEVPCAAVLPGKPGDRNCPEFPPPDRTLGCWATDAAPAAGLCGAGSEPSIAPTSCAGNLPSRPPPLLSPLLASRNPCPWHYLHLSGSHNTLAPTCFKAKLHRKRGSQPPDMASALTDRTSRAPSTYTYTSRPRALPCQRSRYRDSLTQPDEEPMHYGNIMYDRRVIRGNTYALQTGPLLGRPDSLELQRQREARKRALARKQAQEQLRPQTPEPVEGRKHVDVQTELYLEEIADRIIEVDMECQTDAFLDRPPTPLFIPAKTGKDVATQILEGELFDFDLEVKPVLEVLVGKTIEQSLLEVMEEEELANLRASQREYEELRNSERAEVQRLEEQERRHREEKERRKKQQWEIMHKHNETSQKIAARAFAQRYLADLLPSVFGSLRDSGYFYDPIERDIEIGFLPWLMNEVEKTMEYSMVGRTVLDMLIREVVEKRLCMYEHGEDTHQSPEPEDEPGGPGAMTESLEASEFLEQSMSQTRELLLDGGYLQRTTYDRRSSQERKFMEERELLGQDEETAMRKSLGEEELS

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q86UC2-1; Sequence=Displayed; Name=2; IsoId=Q86UC2-2; Sequence=VSP_030128

Alternative Sequence

212..307; Missing (in isoform 2)

3D Structural Models

3D Structure

Electron microscopy (1)

Domain & Motif Annotations

Compositional Bias

153..162; Polar residues

Coiled Coil

215..239; 331..385

Region

134..186; Disordered; 225..249; Disordered; 354..375; Disordered; 473..498; Disordered; 526..560; Disordered

Protein Families

Flagellar radial spoke RSP3 family

Sequence Similarities

Belongs to the flagellar radial spoke RSP3 family.

Clinical Relevance1

Antibody

HPA039109

Supporting Publications2

PMID	Title	Abstract
34265469	Proteomic Landscape of Exosomes Reveals the Functional Contributions of CD151 in Triple-Negative Breast Cancer.	Furthermore, utilizing quantitative proteomics approach to reveal the proteomes of CD151-deleted exosomes and cells, we found that exosomal CD151 facilitated secretion of ribosomal proteins via exosomes while inhibiting exosome secretion of complement proteins. Moreover, we proved that CD151-deleted exosomes significantly decreased the migration and invasion of TNBC cells. Most importantly, we found that the tetraspanin CD151 expression levels in TNBC-derived serum exosomes were significantly higher than those exosomes from healthy subjects, and we validated our findings with samples from 16 additional donors. This is the first comparative study of the proteomes of TNBC patient-derived and CD151-deleted exosomes.
36573687	Proteomic and phosphoproteomic landscape of salivary extracellular vesicles to assess OSCC therapeutical outcomes.	No abstract available