Protein detail

ARG28

Rho guanine nucleotide exchange factor 28 (190 kDa guanine nucleotide exchange factor) (p190-RhoGEF) (p190RhoGEF) (Rho guanine nucleotide exchange factor)

Entry name ARG28	UniProt ID Q8N1W1	EVMP confidence score 0.38
Supporting publications (n) 3	Transmembrane count	Protein classification Predicted intracellular proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

Rho guanine nucleotide exchange factor 28 (190 kDa guanine nucleotide exchange factor) (p190-RhoGEF) (p190RhoGEF) (Rho guanine nucleotide exchange factor)

Protein Class

Predicted intracellular proteins

Protein Function

Predicted intracellular proteins

Ensembl

ENSG00000214944

Entrez Gene Symbol

ARHGEF28

Gene Synonym (3)

p190RhoGEFRGNEFRIP2

Gene Description

Rho guanine nucleotide exchange factor 28

Chromosome

Position

73626158-73941993

Supporting publications (n)

EVMP confidence score

0.38

Fluorescence & Localization2

Cell SpecificCone photoreceptor cellsSingle-Nuclei Brain Specificleukocyte

Function & Pathway8

Protein Function

Predicted intracellular proteins

Cellular Component (2)

Molecular Function (4)

Biological Process (3)

KEGG

hsa05135 Yersinia infection

Reactome (8)

Canonical Pathways (3)

M269 Pid ras pathway
M88 Pid cd8 tcr pathway
M34 Pid tcr pathway

Mediation Categories

Receptor-signaling mediation

Relations & Evidence12

Ligand-Receptor Signaling (6)

6 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
plasma_membrane	plasma_membrane	UniProt_location	No	No	No	No	No
plasma_membrane	plasma_membrane	OmniPath	No	No	No	No	No

Regulatory Interaction Network (4)

4 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
GNAQ	P50148	ARG28	Q8N1W1	Yes	Yes	No	SIGNOR	SIGNOR:25922072
FAK1	Q05397	ARG28	Q8N1W1	Yes	Yes	No	KEGG-MEDICUSWangSIGNOR	SIGNOR:21643014
ARG28	Q8N1W1	RHOA	P61586	Yes	Yes	No	KEGG-MEDICUSWangHINTSIGNOR	HINT:29196061 SIGNOR:32203420 HINT:32203420
GNA13	Q14344	ARG28	Q8N1W1	Yes	Yes	No	SIGNOR	SIGNOR:25922072

Protein Complex Composition (1)

1 record.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
	ARHGEF28 RAC1	P63000 Q8N1W1	2:2	PDB	PDB:6bc1

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential Ultracentrifugation	Mass spectrometryWestern blottingFlow cytometry	3	38225453 34202855 40091455

Sequence, Structure & Domains13

Sequences

Length

1,705

Mass

191,891

Sequence

MELSCSEAPLYGQMMIYAKFDKNVYLPEDAEFYFTYDGSHQRHVMIAERIEDNVLQSSVPGHGLQETVTVSVCLCSEGYSPVTMGSGSVTYVDNMACRLARLLVTQANRLTACSHQTLLTPFALTAGALPALDEELVLALTHLELPLEWTVLGSSSLEVSSHRESLLHLAMRWGLAKLSQFFLCLPGGVQALALPNEEGATPLDLALREGHSKLVEDVTNFQGRWSPSFSRVQLSEEASLHYIHSSETLTLTLNHTAEHLLEADIKLFRKYFWDRAFLVKAFEPEARPEERTAMPSSGAETEEEIKNSVSSRSAAEKEDIKRVKSLVVQHNEHEDQHSLDLDRSFDILKKSKPPSTLLAAGRLSDMLNGGDEVYANCMVIDQVGDLDISYINIEGITATTSPESRGCTLWPQSSKHTLPTETSPSVYPLSENVEGTAHTEAQQSFMSPSSSCASNLNLSFGWHGFEKEQSHLKKRSSSLDALDADSEGEGHSEPSHICYTPGSQSSSRTGIPSGDELDSFETNTEPDFNISRAESLPLSSNLQSKESLLSGVRSRSYSCSSPKISLGKTRLVRELTVCSSSEEQRAYSLSEPPRENRIQEEEWDKYIIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKTKSKDAKDKEKLNRHQFAPGTFSGVLQCLVCDKTLLGKESLQCSNCNANVHKGCKDAAPACTKKFQEKYNKNKPQTILGNSSFRDIPQPGLSLHPSSSVPVGLPTGRRETVGQVHPLSRSVPGTTLESFRRSATSLESESDHNSCRSRSHSDELLQSMGSSPSTESFIMEDVVDSSLWSDLSSDAQEFEAESWSLVVDPSFCNRQEKDVIKRQDVIFELMQTEMHHIQTLFIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHRHFFYSMKERRQESCAGSDRNFVIDRIGDILVQQFSEENASKMKKIYGEFCCHHKEAVNLFKELQQNKKFQNFIKLRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKERTEEHKDLRKALCLIKDMIATVDLKVNEYEKNQKWLEILNKIENKTYTKLKNGHVFRKQALMSEERTLLYDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNNWMRRIQQAVESCPEEKGGRTSESDEDKRKAEARVAKIQQCQEILTNQDQQICAYLEEKLHIYAELGELSGFEDVHLEPHLLIKPDPGEPPQAASLLAAALKEAESLQVAVKASQMGAVSQSCEDSCGDSVLADTLSSHDVPGSPTASLVTGGREGRGCSDVDPGIQGVVTDLAVSDAGEKVECRNFPGSSQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHRLVLQQQEGLSLGHSILRGGPLQDQKSRDADRQHEELANVHQLQHQLQQEQRRWLRRCEQQQRAQATRESWLQERERECQSQEELLLRSRGELDLQLQEYQHSLERLREGQRLVEREQARMRAQQSLLGHWKHGRQRSLPAVLLPGGPEVMELNRSESLCHENSFFINEALVQMSFNTFNKLNPSVIHQDATYPTTQSHSDLVRTSEHQVDLKVDPSQPSNVSHKLWTAAGSGHQILPFHESSKDSCKNDLDTSHTESPTPHDSNSHRPQLQAFITEAKLNLPTRTMTRQDGETGDGAKENIVYL

Alternative Products

Event=Alternative splicing; Named isoforms=6; Name=1; IsoId=Q8N1W1-1; Sequence=Displayed; Name=2; IsoId=Q8N1W1-2; Sequence=VSP_032140, VSP_032141; Name=3; IsoId=Q8N1W1-3; Sequence=VSP_032142, VSP_032143; Name=4; IsoId=Q8N1W1-4; Sequence=VSP_032144; Name=5; IsoId=Q8N1W1-5; Sequence=VSP_044737, VSP_044738; Name=6; IsoId=Q8N1W1-6; Sequence=VSP_032143

Alternative Sequence

1..28; MELSCSEAPLYGQMMIYAKFDKNVYLPE -> MDSDSDSPFNYSWPSFPKMKIRRRTSKQ (in isoform 5); 29..341; Missing (in isoform 5); 583..602; EQRAYSLSEPPRENRIQEEE -> GKHHLTFGFENTLFQNRFFS (in isoform 2); 603..1705; Missing (in isoform 2); 1282..1325; Missing (in isoform 3); 1649; N -> NGSSMTKCSCTLTSPPGLWTGTTSTLK (in isoform 3 and isoform 6); 1650..1705; DLDTSHTESPTPHDSNSHRPQLQAFITEAKLNLPTRTMTRQDGETGDGAKENIVYL -> GN (in isoform 4)

3D Structural Models

Helix

1052..1060; 1077..1080; 1138..1140; 1173..1184

Beta Strand

1066..1068; 1074..1076; 1088..1096; 1102..1109; 1114..1120; 1123..1126; 1134..1136; 1141..1145; 1152..1157; 1164..1169

3D Structure

X-ray crystallography (2)

Domain & Motif Annotations

Compositional Bias

501..510; Polar residues; 640..649; Basic and acidic residues; 710..721; Polar residues; 759..775; Polar residues; 777..791; Basic and acidic residues; 1191..1207; Basic and acidic residues; 1641..1655; Basic and acidic residues; 1656..1669; Polar residues; 1688..1699; Basic and acidic residues

Coiled Coil

1488..1525

Zinc Finger

652..699; Phorbol-ester/DAG-type

Domain (FT)

849..1044; DH; 1086..1188; PH

Region

287..316; Disordered; 473..524; Disordered; 630..649; Disordered; 710..800; Disordered; 1187..1207; Disordered; 1295..1304; Interaction with PTK2/FAK1; required for regulation of axonal branching and synapse formation; 1312..1339; Disordered; 1372..1383; Mediates cytoplasmic retention and interaction with YWHAH; 1425..1705; Interaction with microtubules; 1496..1527; RNA-binding; 1566..1579; Mediates cytoplasmic retention and interaction with MAPK8IP1; 1638..1705; Disordered

Clinical Relevance2

Drugs

METHYLPHENIDATE HYDROCHLORIDE

Antibody

HPA037602

Supporting Publications3

PMID	Title	Abstract
38716512	Assessment of urine sample collection and processing variables for extracellular vesicle-based proteomics.	No abstract available
40189497	Small extracellular vesicle-based one-step high-throughput microfluidic platform for epithelial ovarian cancer diagnosis.	No abstract available
41068253	Identification of plasma extracellular vesicle protein biomarkers in diabetic retinopathy progression.	No abstract available