Protein detail

ENAH

Protein enabled homolog

Entry name ENAH	UniProt ID Q8N8S7	EVMP confidence score 0.38
Supporting publications (n) 1	Transmembrane count	Protein classification Predicted intracellular proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

Protein enabled homolog

Protein Class

Predicted intracellular proteins

Protein Function

Predicted intracellular proteins

Ensembl

ENSG00000154380

Entrez Gene Symbol

ENAH

Gene Synonym (3)

FLJ10773MENANDPP1

Gene Description

ENAH actin regulator

Chromosome

Position

225486765-225653142

Supporting publications (n)

EVMP confidence score

0.38

Fluorescence & Localization4

Tissue Specificbone marrowCell SpecificB-cellsSingle-Nuclei Brain Specificleukocyte

Function & Pathway6

Protein Function

Predicted intracellular proteins

Cellular Component (9)

Molecular Function (4)

KEGG (4)

Reactome (5)

Mediation Categories (3)

Adhesion and uptake mediationFusion and delivery mediationImmune mediation

Relations & Evidence15

Ligand-Receptor Signaling (7)

7 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
actin_regulation_adhesome	intracellular_intercellular_related	Adhesome	Yes	No	No	No	No
intracellular_intercellular_related	intracellular_intercellular_related	OmniPath	Yes	No	No	No	No

Regulatory Interaction Network (3)

3 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
KGP1	Q13976	ENAH	Q8N8S7	Yes	No	Yes	SIGNOR	SIGNOR:15066263
RAPH1	Q70E73	ENAH	Q8N8S7	Yes	Yes	No	SIGNOR	SIGNOR:20417104
BAIP2	Q9UQB8	ENAH	Q8N8S7	Yes	Yes	No	HPRDWangSIGNOR	SIGNOR:11696321 HPRD:11696321

Protein Complex Composition (5)

5 records.

Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs
ARC CBX5 ENAH GLOD4 GOT1 HPF1 NUP42 PPIL3 RAE1 RBM4B RPS21 SUPT3H TXN WDR82	O15504 O75486 P10599 P17174 P45973 P63220 P78406 Q6UXN9 Q7LC44 Q8N8S7 Q9BQ04 Q9H2H8 Q9HC38 Q9NWY4	1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC9762
ENAH PTPN1	P18031 Q8N8S7	1:1	PDB	PDB:9c66
ENAH	Q8N8S7	2	PDB	PDB:5n91PDB:5nd0PDB:5nduPDB:5ncfPDB:6xxrPDB:4my6PDB:5negPDB:6rd2PDB:5najPDB:5n9pPDB:5n9cPDB:5ncgPDB:6xvtPDB:5nbx
ENAH TES	Q8N8S7 Q9UGI8	4:4	PDB	PDB:2iyb
ACTL7A ENAH TES	Q8N8S7 Q9UGI8 Q9Y615	1:1:1	PDB	PDB:2xqn

Sequence, Structure & Domains18

Sequences

Length

591

Mass

66,510

Sequence

MSEQSICQARAAVMVYDDANKKWVPAGGSTGFSRVHIYHHTGNNTFRVVGRKIQDHQVVINCAIPKGLKYNQATQTFHQWRDARQVYGLNFGSKEDANVFASAMMHALEVLNSQETGPTLPRQNSQLPAQVQNGPSQEELEIQRRQLQEQQRQKELERERLERERMERERLERERLERERLERERLEQEQLERERQERERQERLERQERLERQERLERQERLDRERQERQERERLERLERERQERERQEQLEREQLEWERERRISSAAAPASVETPLNSVLGDSSASEPGLQAASQPAETPSQQGIVLGPLAPPPPPPLPPGPAQASVALPPPPGPPPPPPLPSTGPPPPPPPPPLPNQVPPPPPPPPAPPLPASGFFLASMSEDNRPLTGLAAAIAGAKLRKVSRMEDTSFPSGGNAIGVNSASSKTDTGRGNGPLPLGGSGLMEEMSALLARRRRIAEKGSTIETEQKEDKGEDSEPVTSKASSTSTPEPTRKPWERTNTMNGSKSPVISRRDSPRKNQIVFDNRSYDSLHRPKSTPLSQPSANGVQTEGLDYDRLKQDILDEMRKELTKLKEELIDAIRQELSKSNTA

Alternative Products

Event=Alternative splicing; Named isoforms=3; Name=1; IsoId=Q8N8S7-1; Sequence=Displayed; Name=2; IsoId=Q8N8S7-2; Sequence=VSP_010564; Name=3; Synonyms=Deltav6; IsoId=Q8N8S7-3; Sequence=VSP_053772, VSP_053773

Alternative Sequence

268..304; Missing (in isoform 3); 513..533; Missing (in isoform 3); 514..534; Missing (in isoform 2)

3D Structural Models

Turn

18..21; 41..44; 53..55

Helix

26..28; 94..110

Beta Strand

3..17; 22..25; 32..40; 45..52; 58..64; 70..72; 74..81; 82..85; 86..93

3D Structure

X-ray crystallography (27)

Domain & Motif Annotations

Compositional Bias

115..136; Polar residues; 221..264; Basic and acidic residues; 275..305; Polar residues; 311..323; Pro residues; 330..373; Pro residues; 432..443; Gly residues; 479..491; Polar residues; 499..509; Polar residues; 538..549; Polar residues

Repeat

156..160; 1; 161..165; 2; 166..170; 3; 171..175; 4; 176..180; 5; 181..185; 6; 186..190; 7; 191..195; 8; 196..200; 9

Motif

400..403; KLKR

Coiled Coil

135..265; 557..587

Domain (CC)

The EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization.

Domain (FT)

1..111; WH1

Region

115..146; Disordered; 156..200; 9 X 5 AA tandem repeats of [LMQ]-E-[QR]-E-[QR]; 221..379; Disordered; 391..588; EVH2; 391..411; EVH2 block A; 405..549; Disordered; 442..459; EVH2 block B; 554..588; EVH2 block C

Protein Families

Ena/VASP family

Sequence Similarities

Belongs to the Ena/VASP family.

Clinical Relevance4

Antibody (2)

HPA028448 HPA028696

Interaction Protein (4)

ENSG00000075624ENSG00000125753ENSG00000135269ENSG00000159840

Interaction Count

Interaction Dataset (3)

biogrid_opencellintact_biogridintact_biogrid_opencell

Supporting Publications1

PMID	Title	Abstract
27487081	Comparative Proteomic Analysis of Extracellular Vesicles Isolated by Acoustic Trapping or Differential Centrifugation.	No abstract available