Protein detail

LRTM3

Leucine-rich repeat transmembrane protein 3 (Coiled-coil domain-containing protein 168)

Entry name LRTM3	UniProt ID Q8NDH2	EVMP confidence score 0.50
Supporting publications (n) 1	Transmembrane count 1	Protein classification Predicted membrane proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information12

Protein Names

Leucine-rich repeat transmembrane protein 3 (Coiled-coil domain-containing protein 168)

Protein Class

Predicted membrane proteins

Transmembrane

37..57; Helical

Transmembrane Count

Ensembl

ENSG00000175820

Entrez Gene Symbol

CCDC168

Gene Synonym (2)

C13orf40FLJ40176

Gene Description

Coiled-coil domain containing 168

Chromosome

Position

102729367-102759072

Supporting publications (n)

EVMP confidence score

0.50

Fluorescence & Localization5

Cell SpecificErythrocyte progenitorsSingle-Nuclei Brain Specificendothelial cellBlood Cell Specificnon-classical monocyteSecretome LocationSecreted to bloodSecretome FunctionReceptor

Function & Pathway3

Cellular Component

GO:0016020 membrane

Biological Process (3)

Mediation Categories

Other mediation

Relations & Evidence5

Ligand-Receptor Signaling (4)

4 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
transmembrane	transmembrane	UniProt_location	No	No	No	No	No
transmembrane	transmembrane	UniProt_topology	No	No	No	No	No
transmembrane	transmembrane	UniProt_keyword	No	No	No	No	No
transmembrane	transmembrane	OmniPath	No	No	No	No	No

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion Chromatography	Mass spectrometryMass spectrometry [LTQ-FT Ultra]Western blotting	4	36963204 37288703 37368911 38204202

Sequence, Structure & Domains6

Sequences

Length

7,081

Mass

801,912

Sequence

MSKQYYSFKKGVGSGLEDNTFMTLWDFLESWIIQNDWVAIFFIILLGIIFEIILMKACASFWKKPTLPEKGSSDVQETEDSCPKSRKLAPENWSVINSSSGERVGTFLEKRITSSLTSEEKECNFEDRILFSREILWSGTSESEDQVSPSSESHVPSSNGISSSLPLFYSEVEETCLSHTEHPDREYETIQFSSKKLFSMMKTNKNKNSGFSSDLSFSASRFTVENEDLDVAPCPLAHLFLSRDQVRLLEENVRNQIPSKPKTKLGSRTTYQCSRSQESLNQNQPSVGMVISVQAQDSFPGQNAFQNQGLYEVQFTSQAQYINHNQESIKSQPESKASNFAQPEDVMKKPFSSSTQDSFQSQDLDRNQHFVEVPSIVEAKYSVKGLESDEHLGEDQHCVWFIDSNKVKYSIKGQDTIFKNAEFLVLTLNPNLVTEDMPQLRSVKAQGQQQIVSSELNQDSVYSSVPLLSTIKGQKNRRKTPDSKSKLSLNVPSLKAKKTPTSQVFQITVCHTLKNRNELGCKNNTEKKELHERKDISDIALHLISVSKLILPYVKNYSRKQLVKVMPGLIKCGHFLQKQNKSPDTEKINYAGPLEETGISDITKKEKEYDKENKRLKNISPKMLPQLEQSFMVNTVQLKAPCLLVETNGKSKESLKDSITQAKGIGITEFHVLNSKKPFDLHIPKHKTSLEEAISKPMQKLVSSPEMESNNRMKIQEDLQSSENSHLQLSNGEELPTSTPKTQRCFPRENTQKQKDFLELVLELSNVGLLISPGSKMHKSSEELEAIKIQVNTESVNLKESKPLILNVTEDSDLRESEELECNTGSNITNMHQDKETSDAFHSATYTTISQLPDTETHSISKAKADTLRIIRLSHSASKQEKLPDEKETQNAEYIDKSCTFKKPQQCDRKEQEKEANSELTQGFRFSIHLKQKPKYVKFQMEQISSGSSKAPNKEQEVQPQTLSTQTILENSPCPMMDPFQVEKVKQSTDRPTDRESAGDPKNPLTMPENLPVGELLIETTEYSVPFGGNLQKTTDSHIAEEKEDVKRYLPAVALGSFNNHLLTLPYFKRQEIKKKLSETKSVLSVKYVIMKVKKPAISLMPYINICGTSNHRKKMGGNFEIIIKQILQDKIAAGMLLNVIYPPMSILPNTRMYSRLNAENHSHIKLVQEESQIEREEKYPYFINEGNESQNTLDAKLQDEVKGVKETLPKAVLHDSCNLGLDAHLEKEIKTEKEMHQPIPFTETIIESVVSPIMELSHAENVKSTQKTQTDCKCTADSETPSPISGKSLIGDPLNQTRESYIPSNGSDTREMGYCFAEEKTEIPKDLPATSPETFNYCTPVLSCSKVMKKRVTFALTTSTAKPKCVNTKAVKPSISETVSVTSHRKKSELDFKTKFKKINQTKGLVPECLNTLCSPMHSRLQREFCLPASQLKQGETADKTYTDVFAKNSISHDREEKLQDGKEEEHKVLLEAAPQLSQHLGSEAGQMKEIHLESDPVLNCLTLELHINGQRLQHQTGFEQTTLETSLQMGPLEAEELQKANETENDIKVLGGPKIPPPKALQALENSDGLILNAYQKDNELVKSDEELNQPGSTNIQVQPQTHFTQTILKSTSCPTLDQFPFEKVESHVRFSPLKSGEAKVDEIIFYAREGGISSDSSHQKEQAGGTEKKETAIFGSCMPALSTPKTTRNLKQFSDMKTLVNPKCGIIKAKKPSISYMLNIRAGAGPKRRKELSCNLTTKMKELHQGKKGVDETYAFLTMTPDINKYSKVETEKDTLREKRLSSTQVKQDTSPHEDSITSRDIKETLLQDEEQEERKQEALLKVIPQHLQHFMFRSGQGKDLDFHKLENQGSRKILFVTKQDVPQQLQPAEPIQREETKKCLQTQNGTICTVNSKLLPLKSEDSVNGEVLTGAIKRGVPTDRKCMGEQHNSGKGEKAEFNKDLQATVLELQKSPHGGEAQKANLTDMESGSSNAMNMNVQHEREDKNIQKMLTESVPCYSQHLRFSTHQMKDPDPCKSGSEPKSPEGRSWNLSHIVQKTKQETHFRETVLEPISGYMMKQSPHMQEGIKCMEGLKTSFPKTGKSKIGSIPRDTPWDENPRRKWDSSISEKTAWNQKNLQTVLKPLDFSSLMSSEYESRSYTLEFIGKKSMSPKCVTLKAKQLRILQLFNIIRYSTENHRKKKQHRFKYKMKGKQWYTSIGEALLSATEYAKSTTSKSMIDKLLYNTAARCILSNRTRRQNLDGHITEEKEEVQENVAAIFLGLLDFFMPVLSDSKNQRNTAQLSEKEIIFNAKCLTMKEKKSSISQIHKINRESTRKHRKKCKSYLKTVSNRKCQENHGHITEEEEEVQENSPATFLGPLDFFMPVLSDSKNQINTIQLSERKIILNPKCLTMKEKKPPISQIHKISGQFTTKHRKKLESNLKTKLKAMWQGENVADTFPNTTSFTPDSSDIKRQSGFQTEIDMRISGLSHTQPTQIESLAEGIARYSDPIDKRRTSNLVKGAKLHDRESGEEKQEHLTEMDPFYAENFMANTYLRKDRHLGKSEDVLLGETFFSKSQIYKGNSEKNVKIEKNKNGKESLKVGLARMEKSDNCAELSEATDDAISNKYDKQNIGHSVLKENAFCNLAAIVPDSVGRHSPASEEMKRQNGRLKMADRSSPQGRPLQAKQSAVSQSPDTAGYAVVSNNKEQKQNFKAQKTEAEVDLIDQEAKINVAEEFNPESVFFSKIHPLQIENKKEFKTADWKTRADPKTFALPKKQQELCVSGTIWSYPNPYTSISPKIIRHKDKAKTADVESTMHTKQIKLKAKRITVSQLLEYGTASNKKELRGNIQQQKSFQLSKNAVHRVLKAVYDSGYCVSSIKKLTEVKMEKDKPKDRTCILPQPKLEKPLKEMQRSLSGCTDMSSILRKQEQDIREKEQKHQSISEDISQYYIGPLRISSQQINYSSFDAPRIRTDEELEFLIAQRAKEKDVGIAKHSVSIPWEREGSKRLDIPLNSKGQNIFFTELDTSQQKTCQEQELLKQEDISMTNLGSMACPIMEPLHLENTGKVTEEEDVYINRKISSHVLGKEGLKETDIFVGSKGQKFLCTNSEVQHKVPAEQKEQVNPDHVPESILDSESFLSKDPLHLKQAVNTARKENVTISESFNENLWGKEQSKLDITLKSNRQKMDFSKKLRMKHLSNYYQNKENILESVLPCILHQLYIENPKKEGSAEEIMSSKVLSPMVEKASHEVGIPVDQPPCSEGIHLNIKGRKEHPQESTHEAFPASVSHSLMDVLQIKSPKVKKALKAINSLGYLTSNTKGIGLLFPRQAEKEEKYTYKALPKPASHSKTDLFQFNASMQQEKLDAMDIPHYDYLTSQTREAVKQMDVIVGYTQNSKKRQDLLKTGQKWQYLPISYENFWEHISCPQKYPCLLQHLMPQEKEALSEGGNLSSRTPGLDLFSADQLSTITKNRLEWIVPLISPRQMKKQDSMLPLGSYHKTIKYASLLFPKGMKSSDGVQVFDLISNNSSPKLRLGKKIETQKANEKVQKEVCLPITLHSLSASMPILQESKGQKDSVEQVIRKGVICHKRRTSKWKKSVFSHILNTSDCGASSNRLEMQWNMTDKMVNVKHRMSEIDLVAAKICESILSLPHFKLNKETIDGVISSNVKSTKQHISQGKNDRVKAMDMKRIKSPNIILKPRKSSLSHILSIKEFPLLLDIIKQEGKMQEGKGKSSMKLTNLCTSLPSLSHSNSNSRTKAGKDKSGTLKGCLPPLKLQASSNARRVSSAESINRDSLSNVIESKCFPQKKKEDRENIVDVKDVMGLKCITLKGKKSLFRHLLHGKEPQRSNKKLEKMTQEDESNLNVVQNKLCASILSPPHLEWNPRIKEVYMRGITRFCLSSSTQQELSDTMEKCEQPIDDSLSSIEKAKHMPQKDKDRVEKALEKIMHSKRIALEVKQPSIFQELELNIKEKGGKIQEDKEVEIWSKPFASISFLPYSKVGTIEGEEAMRIKMRSSFSQPNLQESSDTEKTAYEKCISDNISNSVKKALESILQKEQRQKMEKIRALKKMKSSISQGIQLDIKEQEKRIEHIKGEPSVLLTNACASIPSPSHLQLDTRREKAEYVTEITRYYLPELSHQKSSEAGEKADGVASKGDITIKVQKAKDYMQQKEDDEVKISAKKDIMHPEDKGLKAKKALSQDLPLNTKEPGKMDQEAQEQGKEDREGEEQGKEDRRGAGQEKVDREDKEQGKMDHEVEEQQKADGVGIEQGKMDGDKNEQERVLFLYLPSNSSLTHYILDTRIEGEEDQQGIIRPGILQPRHQKSSETGKKANGVPSEGDSASEVQKAKDYMQQKEEDEVKISAEKDLMHPEDKDLKGKKALSQNLPLNPKEPGKRDGEGQEQGKEDGEGEEQGNRDGETEEQQQADGVGTEQEKRDGHKSKQETVLFLHLPSESSLTCYELNTRKEGEEDLQGIIKSATLQLRQQKSFDAGKIAHTKSFGVDSSNDVKTVQEYKPQKEVDRGKTVSVDYIMQPEGTIFEAEQLSLPHTLNIPGSSGSKTREVLTNIKEKLRHVQERKSELDVFLTIPSLSHCKLDKRTAGKKEEQGVTRSFLPPSWHMESSDTGKLKYTLSYLNDITGDSNRTKYMAQIQKDKANISEKSVMHPEYIAVKAEKSPLSHILKTKELQVNISQQGEKAQEGEVEIVVLLSKTCPFVTSSAFLELDSIKEEEGEPRITRSFMPHLEIQESLPSRQTAPTKPTESLVKKEKQLLPQKEDRVQTVSMHGLMHPNGAVFKAKTSAPPQVFSITEHSPLSKRKEPQWGMKERAGQKQDRTGRPHVILTKTHPFMPSLSHHRFSPSQPKLPISSGAGKSRLANSNEGISSHKVILKANQQMPYKEAKDRVKIEGREGRILPKRIHLRAEALPLALLCNGKNYSLHIEEQGEGVQESKKEPGVVPRKSASFPPPPFYLNCDTRRNEKEGTLGKTQFSFPPLKIQDSSDSGKKAYTESLHGYTLSNSKGPVQPTAQGEEKGGLRIDMEDKMLPKCTDLKAKQLLLSDILNTKKLQWKSKEQKRKIQEDKNKQVKGLPSINTSLLTPPYLKFDTTEGQENVIRIAKVSLPQSRSKESSDAGRIACPEATHGELSSDVKQLKAHLLQKEEKDREKVADMTSVLDPNKMYLKAKKSPVLHTHSFSDLQWKTREQEEEKVQKVKSGPGVMLSKSPSRSSPLHLNVNTGFQEESIPILTRPSFPLVKLQVSPDTEGGTCIRPIAGDILIYLQKGKHVSQNKEEDDVQIVSILIFPKHQEEKVQECEGEPGVVLTKSTSLPSLSQLELDKETHLGNEMLRLKRPILRRISHIGETVHRESVVGDIPKDVKNEKQHIPQKEERNQKKIIDMRGTDITLKSKKSPRSCMLHRTELHVNIGGQGRKEHEGQDKPPGMIQRKMCILFSKPLPSNLKLERATHADEERLGGKTSFVLPLMPSALPDTEKTADAEARSGDVRKGKPHRSQKENRHEVKTIDMRFRIHCQEARISPMSHILNAKELVLNINKLEKKVHKDKDEACVVLSRTFLSIPSAPPLYLDSGNKTDKDTPGITGSSCPQRTLHVPSNTQKITNRDSVEGVDKNVVKQAEQYVPRPEAEQQLTSNFMISVQQRNQPSRVRSEEDLNQLVLNSRDEDIYFTGFGTIRSGKRPEWLFTGKKAQPVKYKTETLTAFLSYPTMDATKMGGLEEDTEIMDNLNHKISPKASVSLIRKISKELYVTLGTPANSKGFSVSERYAHQQETSSKVSPELAGSCKFDKPKEDGQSNDRISKMFSPKVLAPQTKGSLKKISIVTNWNAPQNIEEQDIVMKKQVIRRCEHGHKTRTNTILSKFPLQSGKQKTPSETDVDKKTTAHLSLQMLPGIHMDMTEIDPAKGGRKQALLISEQEEGVLEFLPKSLFPPWTFQFQSGDLEEKHQTDANTNINLEQKKLEMDNDSTVNQKEGKLKIGTNRALHLQEEKTEMHKARTANLEKERGRMDTSSSAHPHLLSLKAEESQMKTQVITHRENSRLIMQKQKKELEASNAKQSIQLQKLFQRNVLDSFYSYVPLSPKRKDQKGRLTIRDLKRELSTKYLTMKIQNHPIPQMLNITGRGTPSNRKKLEYDVKLKNIASWSKDVSGIFIRSLSISIMRSPHTDPKTNLEREKRICLPKFQEKSPNTSEMSKRDTLTIVKGEQNFTNTVPQDPQPFAVDKQQMQKLPNVKSEANLRSEMNKKYLKAQTKERIVPEHDVSRIIKKPDLRIIEQEEKILKRILTPTECPSMLEDPKLPKQRDQSEPVWDMTTQKVQQQKAFPGTVPIPPQVKSSEVKIVADSTNAEHLLPICEATKAISESQVKNMIQDKVSSDKLDNIQAYKPDDLKSPPFPEGPDTISTAIYPKTQHKSLLEQFTPKEKNKLTSHLESKALEIQLNLIPEMARKSLQMFNFYPKGTISKDNSWRFYSRHKTMNFMSLEGTDTIEPNSKHKHQKDSPLASNMKTLIVDVSSDSEETITKLQSINKLENGTSAVTSASEMLLPHTLQNHSVKEKGKLLMHFSVKTLEIQMKAFPRIVRESYAMTSAHERKKPLSNCIHPGFTGPKRQNRILLLSEEKSLHQIDLDLQYKYLRFLLGLPVGSTFPKPNVLPKHSKLNTIAVCKNVNAGGQSGSLSIDTELLEQHISFKKQSPHENSSLIRKFPQPTLVCASDRDLHSPRKKDTQVLSESEFHVTPEKNKQYHVWFQERNTCESVDLRTQRNATGSAVSCETQISEDFVDIQTDIESPADLDECSCLEVSESEECVFLEANSYLSQESENILFELQTGIPLENVYKITTDLKSFYSEDSGSHCTRECRKETLIITPPSCKSHKSRKYRSSSKMKSPDWLCHSSSNTAEIQSRSSSVSFSEEKISWTTKSRTSYSSAPLTESNIKSHLAKNQGKSHRHPESQERKKARSDLFRKNSSHWDHDYSCTHSKGKRDRKKRVYDYESERLDCFQSKHKSASKPHHDDINFYSERKQNRPFFFACVPADSLEVIPKTIRWTIPPETLRKRNFRIPLVAKISSSWNIWSSSKKLLGSLSGSLTTVFHS

Domain & Motif Annotations

Compositional Bias

718..742; Polar residues; 958..970; Polar residues; 981..999; Basic and acidic residues; 1274..1286; Polar residues; 1295..1304; Polar residues; 1773..1784; Basic and acidic residues; 1793..1804; Basic and acidic residues; 1964..1973; Polar residues; 2668..2678; Polar residues; 4121..4133; Basic and acidic residues; 4147..4176; Basic and acidic residues; 4192..4245; Basic and acidic residues; 4329..4361; Basic and acidic residues; 4375..4401; Basic and acidic residues; 4415..4426; Basic and acidic residues; 4731..4743; Polar residues; 4746..4756; Basic and acidic residues; 4798..4817; Basic and acidic residues; 5203..5212; Polar residues; 5469..5496; Basic and acidic residues; 5779..5792; Basic and acidic residues; 6860..6871; Basic residues; 6937..6950; Basic and acidic residues

Repeat

233..256; LRR 1; 420..445; LRR 2; 865..890; LRR 3; 1050..1075; LRR 4; 1501..1527; LRR 5; 2373..2397; LRR 6; 2727..2749; LRR 7; 2832..2855; LRR 8; 2862..2889; LRR 9; 3433..3458; LRR 10; 3630..3653; LRR 11; 3875..3898; LRR 12; 5311..5336; LRR 13; 5522..5545; LRR 14; 5901..5924; LRR 15; 6259..6282; LRR 16; 6419..6442; LRR 17; 6552..6575; LRR 18; 6613..6637; LRR 19; 7012..7036; LRR 20

Region

717..745; Disordered; 943..1009; Disordered; 1274..1304; Disordered; 1773..1804; Disordered; 1954..1973; Disordered; 2008..2031; Disordered; 2083..2103; Disordered; 2637..2680; Disordered; 3730..3756; Disordered; 4119..4260; Disordered; 4293..4428; Disordered; 4729..4756; Disordered; 4794..4817; Disordered; 4831..4859; Disordered; 4928..4955; Disordered; 4966..4985; Disordered; 5191..5212; Disordered; 5467..5496; Disordered; 5564..5583; Disordered; 5763..5792; Disordered; 6859..6878; Disordered; 6916..6950; Disordered

Clinical Relevance1

Antibody (2)

HPA048184 HPA058793

Supporting Publications1

PMID	Title	Abstract
19837982	Proteomics analysis of A33 immunoaffinity-purified exosomes released from the human colon tumor cell line LIM1215 reveals a tissue-specific protein signature.	A conspicuous finding of this comparative analysis was the presence of host cell-specific (LIM1215 exosome) proteins such as A33, cadherin-17, carcinoembryonic antigen, epithelial cell surface antigen (EpCAM), proliferating cell nuclear antigen, epidermal growth factor receptor, mucin 13, misshapen-like kinase 1, keratin 18, mitogen-activated protein kinase 4, claudins (1, 3, and 7), centrosomal protein 55 kDa, and ephrin-B1 and -B2. Here, we describe an immunoaffinity capture method using the colon epithelial cell-specific A33 antibody to purify colorectal cancer cell (LIM1215)-derived exosomes.