Protein detail

TRPM4

Transient receptor potential cation channel subfamily M member 4 (hTRPM4) (Calcium-activated non-selective cation channel 1) (Long transient receptor potential channel 4) (LTrpC-4) (LTrpC4) (Melastatin-4)

Protein symbol TRPM4	UniProt ID Q8TD43	EVMP score 0.63
Frequency 6	Transmembrane count 6	Protein classification Disease related genesHuman disease related genesPotential drug targetsPredicted intracellular proteinsPredicted membrane proteinsTransportersVoltage-gated ion channels

Basic Information

Protein Names

Protein Class

Disease related genesHuman disease related genesPotential drug targetsPredicted intracellular proteinsPredicted membrane proteinsTransportersVoltage-gated ion channels

Protein Function

Voltage-gated ion channels:Transient Receptor Potential Channels
Predicted intracellular proteins
Potential drug targets
Transporters:Transporter channels and pores
Human disease related genes:Congenital malformations:Congenital malformations of skin
Disease related genes
Human disease related genes:Cardiovascular diseases:Cardiac diseases

Transmembrane

783..803; Helical; 815..835; Helical; 864..884; Helical; 887..910; Helical; 931..951; Helical; 1020..1040; Helical

Transmembrane Count

Ensembl

ENSG00000130529

Entrez Gene Symbol

TRPM4

Gene Synonym

FLJ20041

Gene Description

Transient receptor potential cation channel subfamily M member 4

Chromosome

Position

49157741-49211836

Frequency

EVMP Score

0.63

Fluorescence & Localization

Tissue SpecificesophagusCell SpecificEndometrial ciliated cellsSingle-Nuclei Brain Specificependymal cell

Function & Pathway

Protein Function

Voltage-gated ion channels:Transient Receptor Potential Channels
Predicted intracellular proteins
Potential drug targets
Transporters:Transporter channels and pores
Human disease related genes:Congenital malformations:Congenital malformations of skin
Disease related genes
Human disease related genes:Cardiovascular diseases:Cardiac diseases

Cellular Component

Molecular Function

Biological Process

KEGG

hsa04911 Insulin secretion

Reactome

Mediation Categories

Fusion and delivery mediationImmune mediationMetabolism mediationReceptor-signaling mediation

Relations & Evidence

Enzyme-Mediated Modification

5 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
TRPM4	PRKCA	P17252	S	1,152	phosphorylation	PhosphoSite_MIMPMIMPSIGNORProtMapperSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	ProtMapper:15590641 SIGNOR:15590641
TRPM4	PRKCA	P17252	S	1,145	phosphorylation	PhosphoSite_MIMPMIMPSIGNORProtMapperSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	ProtMapper:15590641 SIGNOR:15590641
TRPM4	CSNK1E	P49674	S	839	phosphorylation	PhosphoSitePhosphoSite_ProtMapperProtMapper
TRPM4	KRT1	P04264	S	839	phosphorylation	REACH_ProtMapperProtMapper	ProtMapper:25441424
TRPM4	CSNK1A1L	Q8N752	S	839	phosphorylation	RLIMS-P_ProtMapperProtMapper	ProtMapper:25231975

Ligand-Receptor Signaling

22 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
receptor	receptor	scConnect	No	Yes	No	No	No
transmembrane	transmembrane_predicted	Phobius	No	No	No	No	No

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Regulatory Interaction Network

2 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
KPCA	P17252	TRPM4	Q8TD43	Yes	Yes	No	PhosphoSite_MIMPMIMPPhosphoSite_norefSIGNORiPTMnetProtMapperSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	ProtMapper:15590641 SIGNOR:15590641 PhosphoSite:15590641
KC1E	P49674	TRPM4	Q8TD43	Yes	No	No	iPTMnetProtMapperELMPhosphoSitePhosphoSite_ProtMapper	PhosphoSite:25231975 ELM:25231975

Protein Complex Composition

1 record.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
	TRPM4	Q8TD43	4	PDB	PDB:8rcrPDB:9b93PDB:9b8yPDB:5wp6PDB:6bqrPDB:9b90PDB:9b94PDB:8rd9PDB:9b8wPDB:8rcuPDB:6bwiPDB:6bqvPDB:9b92

Isolation & Detection Technology

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationUltrafiltration / Tangential Flow FiltrationSize Exclusion ChromatographyImmunoaffinity Capture	R SequencingMass spectrometry	18	33718342 39949490 37702715 32795414 38731868 31222168 26801919 40784529 22106071 37686366 40098346 41201090 41216884 40311616 39835386 40091455 39532703 41315767

Sequence, Structure & Domains

Sequences

Length

1,214

Mass

134,301

Sequence

MVVPEKEQSWIPKIFKKKTCTTFIVDSTDPGGTLCQCGRPRTAHPAVAMEDAFGAAVVTVWDSDAHTTEKPTDAYGELDFTGAGRKHSNFLRLSDRTDPAAVYSLVTRTWGFRAPNLVVSVLGGSGGPVLQTWLQDLLRRGLVRAAQSTGAWIVTGGLHTGIGRHVGVAVRDHQMASTGGTKVVAMGVAPWGVVRNRDTLINPKGSFPARYRWRGDPEDGVQFPLDYNYSAFFLVDDGTHGCLGGENRFRLRLESYISQQKTGVGGTGIDIPVLLLLIDGDEKMLTRIENATQAQLPCLLVAGSGGAADCLAETLEDTLAPGSGGARQGEARDRIRRFFPKGDLEVLQAQVERIMTRKELLTVYSSEDGSEEFETIVLKALVKACGSSEASAYLDELRLAVAWNRVDIAQSELFRGDIQWRSFHLEASLMDALLNDRPEFVRLLISHGLSLGHFLTPMRLAQLYSAAPSNSLIRNLLDQASHSAGTKAPALKGGAAELRPPDVGHVLRMLLGKMCAPRYPSGGAWDPHPGQGFGESMYLLSDKATSPLSLDAGLGQAPWSDLLLWALLLNRAQMAMYFWEMGSNAVSSALGACLLLRVMARLEPDAEEAARRKDLAFKFEGMGVDLFGECYRSSEVRAARLLLRRCPLWGDATCLQLAMQADARAFFAQDGVQSLLTQKWWGDMASTTPIWALVLAFFCPPLIYTRLITFRKSEEEPTREELEFDMDSVINGEGPVGTADPAEKTPLGVPRQSGRPGCCGGRCGGRRCLRRWFHFWGAPVTIFMGNVVSYLLFLLLFSRVLLVDFQPAPPGSLELLLYFWAFTLLCEELRQGLSGGGGSLASGGPGPGHASLSQRLRLYLADSWNQCDLVALTCFLLGVGCRLTPGLYHLGRTVLCIDFMVFTVRLLHIFTVNKQLGPKIVIVSKMMKDVFFFLFFLGVWLVAYGVATEGLLRPRDSDFPSILRRVFYRPYLQIFGQIPQEDMDVALMEHSNCSSEPGFWAHPPGAQAGTCVSQYANWLVVLLLVIFLLVANILLVNLLIAMFSYTFGKVQGNSDLYWKAQRYRLIREFHSRPALAPPFIVISHLRLLLRQLCRRPRSPQPSSPALEHFRVYLSKEAERKLLTWESVHKENFLLARARDKRESDSERLKRTSQKVDLALKQLGHIREYEQRLKVLEREVQQCSRVLGWVAEALSRSALLPPGGPPPPDLPGSKD

Alternative Products

Event=Alternative splicing; Named isoforms=3; Name=1; Synonyms=TRPM4b; IsoId=Q8TD43-1; Sequence=Displayed; Name=2; Synonyms=TRPM4a; IsoId=Q8TD43-2; Sequence=VSP_021442; Name=3; Synonyms=TRPM4c; IsoId=Q8TD43-3; Sequence=VSP_021443

Alternative Sequence

1..174; Missing (in isoform 2); 738..882; Missing (in isoform 3)

3D Structural Models

Turn

63..66; 197..200; 202..205; 306..308; 323..325; 340..342; 356..360; 367..369; 648..650; 681..683; 914..916; 1089..1092

Helix

8..10; 11..14; 99..108; 132..149; 161..175; 247..258; 265..267; 282..293; 309..316; 326..335; 346..355; 374..385; 391..393; 394..402; 407..411; 422..434; 438..446; 451..454; 457..465; 472..479; 503..511; 558..569; 572..580; 585..602; 608..633; 635..641; 654..661; 664..668; 670..680; 690..698; 700..704; 767..777; 779..802; 812..833; 852..860; 863..883; 888..905; 906..911; 917..925; 927..952; 959..974; 980..983; 985..987; 1017..1032; 1034..1049; 1051..1070; 1077..1079; 1080..1087; 1116..1141; 1144..1176

Beta Strand

16..19; 36..38; 41..44; 68..71; 76..78; 79..84; 89..96; 118..122; 127..129; 155..160; 177..179; 187..190; 193..195; 206..208; 230..236; 238..240; 261..263; 268..270; 273..277; 298..301; 343..345; 362..364; 370..372; 416..418; 604..607; 705..708; 807..809; 884..886; 988..990; 995..998; 1000..1002; 1006..1008

3D Structure

Electron microscopy (16)

Domain & Motif Annotations

Motif

975..977; Selectivity filter

Coiled Coil

1134..1187

Domain (CC)

TRPM4, is a temperature-sensitive ion channel, it adopts distinct conformations at different temperatures, markedly influencing where and how ligands interact with it. Decavanadate (a positive modulator), ATP (an inhibitor) and Ca(2+) bind to different locations of TRPM4 at physiological temperatures or at lower temperatures.

Region

1076..1176; Calmodulin-binding; 1136..1141; Mediates modulation by decavanadate and PIP2-binding

Protein Families

Transient receptor (TC 1.A.4) family
LTrpC subfamily
TRPM4 sub-subfamily

Sequence Similarities

Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM4 sub-subfamily.

Clinical Relevance

Disease Involvement

Disease variantPalmoplantar keratoderma

Drugs

NULL

Antibody

HPA041169