Protein detail

ROBO4

Roundabout homolog 4 (Magic roundabout)

Entry name ROBO4	UniProt ID Q8WZ75	EVMP confidence score 0.38
Supporting publications (n) 1	Transmembrane count	Protein classification

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information6

Protein Names

Roundabout homolog 4 (Magic roundabout)

Protein Function (2)

Disease related genes
Human disease related genes:Congenital malformations:Congenital malformations of the circulatory system

Ensembl

ENSG00000154133

Entrez Gene Symbol

ROBO4

Supporting publications (n)

EVMP confidence score

0.38

Fluorescence & Localization4

Cell SpecificAstrocytesSecretome LocationIntracellular and membraneSecretome FunctionTransport

Function & Pathway6

Protein Function (2)

Disease related genes
Human disease related genes:Congenital malformations:Congenital malformations of the circulatory system

Cellular Component (4)

Molecular Function (3)

Biological Process (3)

KEGG

hsa04517 IgSF CAM signaling

Mediation Categories

Adhesion and uptake mediation

Relations & Evidence39

Ligand-Receptor Signaling (36)

36 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
receptor	receptor	GO_Intercell	No	Yes	No	Yes	No
receptor	receptor	HPMR	No	Yes	No	Yes	No
receptor	receptor	ICELLNET	No	Yes	No	Yes	No
receptor	receptor	CellTalkDB	No	Yes	No	Yes	No
receptor	receptor	Ramilowski2015	No	Yes	No	Yes	No
receptor	receptor	LRdb	No	Yes	No	Yes	No
ig_like	receptor	Almen2009	No	Yes	No	Yes	No
roundabout	receptor	Almen2009	No	Yes	No	Yes	No
roundabout	receptor	HPMR	No	Yes	No	Yes	No
receptor	receptor	OmniPath	No	Yes	No	Yes	No

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Regulatory Interaction Network (1)

1 record.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
SLIT2	O94813	ROBO4	Q8WZ75	Yes	Yes	No	HPMRFantom5_LRdbCellTalkDBHPMR_LRdbiTALKHPMR_CellinkertalklrRamilowski2015SIGNORconnectomeDB2020CellinkerWangLRdbHPMR_talklr	Cellinker:31217826 HPMR:15894287 SIGNOR:12941633 connectomeDB2020:15894287 LRdb:15894287 CellTalkDB:15894287 Cellinker:15894287

Protein Complex Composition (1)

1 record.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
Robo4\u2013paxillin complex	PXN ROBO4	P49023 Q8WZ75	0:0	CORUM	CORUM:6927	19855388

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationDensity Gradient CentrifugationSize Exclusion Chromatography	Mass spectrometry	6	39766158 36398564 34817906 28986585 27894104 40089067

Sequence, Structure & Domains10

Sequences

Length

1,007

Mass

107,457

Sequence

MGSGGDSLLGGRGSLPLLLLLIMGGMAQDSPPQILVHPQDQLFQGPGPARMSCQASGQPPPTIRWLLNGQPLSMVPPDPHHLLPDGTLLLLQPPARGHAHDGQALSTDLGVYTCEASNRLGTAVSRGARLSVAVLREDFQIQPRDMVAVVGEQFTLECGPPWGHPEPTVSWWKDGKPLALQPGRHTVSGGSLLMARAEKSDEGTYMCVATNSAGHRESRAARVSIQEPQDYTEPVELLAVRIQLENVTLLNPDPAEGPKPRPAVWLSWKVSGPAAPAQSYTALFRTQTAPGGQGAPWAEELLAGWQSAELGGLHWGQDYEFKVRPSSGRARGPDSNVLLLRLPEKVPSAPPQEVTLKPGNGTVFVSWVPPPAENHNGIIRGYQVWSLGNTSLPPANWTVVGEQTQLEIATHMPGSYCVQVAAVTGAGAGEPSRPVCLLLEQAMERATQEPSEHGPWTLEQLRATLKRPEVIATCGVALWLLLLGTAVCIHRRRRARVHLGPGLYRYTSEDAILKHRMDHSDSQWLADTWRSTSGSRDLSSSSSLSSRLGADARDPLDCRRSLLSWDSRSPGVPLLPDTSTFYGSLIAELPSSTPARPSPQVPAVRRLPPQLAQLSSPCSSSDSLCSRRGLSSPRLSLAPAEAWKAKKKQELQHANSSPLLRGSHSLELRACELGNRGSKNLSQSPGAVPQALVAWRALGPKLLSSSNELVTRHLPPAPLFPHETPPTQSQQTQPPVAPQAPSSILLPAAPIPILSPCSPPSPQASSLSGPSPASSRLSSSSLSSLGEDQDSVLTPEEVALCLELSEGEETPRNSVSPMPRAPSPPTTYGYISVPTASEFTDMGRTGGGVGPKGGVLLCPPRPCLTPTPSEGSLANGWGSASEDNAASARASLVSSSDGSFLADAHFARALAVAVDSFGFGLEPREADCVFIDASSPPSPRDEIFLTPNLSLPLWEWRPDWLEDMEVSHTQRLGRGMPPWPPDSQISSQRSQLHCRMPKAGASPVDYS

Alternative Products

Event=Alternative splicing; Named isoforms=3; Name=1; IsoId=Q8WZ75-1; Sequence=Displayed; Name=2; IsoId=Q8WZ75-2; Sequence=VSP_010658, VSP_010659; Name=3; IsoId=Q8WZ75-3; Sequence=VSP_010654, VSP_010657

Alternative Sequence

24..133; Missing (in isoform 3); 813..1007; Missing (in isoform 3); 932..933; DA -> GM (in isoform 2); 934..1007; Missing (in isoform 2)

Domain & Motif Annotations

Compositional Bias

531..548; Low complexity; 725..756; Low complexity; 763..785; Low complexity

Domain (FT)

32..131; Ig-like C2-type 1; 137..224; Ig-like C2-type 2; 248..345; Fibronectin type-III 1; 347..442; Fibronectin type-III 2

Region

531..551; Disordered; 713..793; Disordered; 806..826; Disordered; 971..1007; Disordered

Protein Families (2)

Immunoglobulin superfamily
ROBO family

Sequence Similarities

Belongs to the immunoglobulin superfamily. ROBO family.

Supporting Publications1

PMID	Title	Abstract
36398564	Differential ultracentrifugation enables deep plasma proteomics through enrichment of extracellular vesicles.	No abstract available