Protein detail

HMHA1

Rho GTPase-activating protein 45 [Cleaved into: Minor histocompatibility antigen HA-1 (mHag HA-1)]

Entry name HMHA1	UniProt ID Q92619	EVMP confidence score 0.50
Supporting publications (n) 1	Transmembrane count	Protein classification Plasma proteinsPredicted intracellular proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

Rho GTPase-activating protein 45 [Cleaved into: Minor histocompatibility antigen HA-1 (mHag HA-1)]

Protein Class (2)

Plasma proteinsPredicted intracellular proteins

Protein Function

Predicted intracellular proteins

Ensembl

ENSG00000180448

Entrez Gene Symbol

ARHGAP45

Gene Synonym (3)

HA-1HMHA1KIAA0223

Gene Description

Rho GTPase activating protein 45

Chromosome

Position

1065923-1086628

Supporting publications (n)

EVMP confidence score

0.50

Function & Pathway6

Protein Function

Predicted intracellular proteins

Cellular Component (7)

Molecular Function (3)

Biological Process (3)

Reactome (7)

Mediation Categories (2)

Immune mediationReceptor-signaling mediation

Relations & Evidence10

Ligand-Receptor Signaling (9)

9 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
ecm	ecm	MatrixDB	Yes	No	No	No	No
ecm	ecm	OmniPath	Yes	No	No	No	No
extracellular	extracellular	OmniPath	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
fbar_actin_dynamics_endocytosis	intracellular_intercellular_related	HGNC	Yes	No	No	No	No
intracellular_intercellular_related	intracellular_intercellular_related	OmniPath	Yes	No	No	No	No

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationDensity Gradient CentrifugationUltrafiltration / Tangential Flow FiltrationSize Exclusion Chromatography	Mass spectrometryMass spectrometry [LTQ-FT Ultra]	21	23161513 28369848 23161513 32795414 23161513 31018934 41216884 32284825 34623918 39766158 36398564 37786918 34817906 28986585 27894104 29045505 38321535 40089067 32089743 39940641 34186243

Sequence, Structure & Domains12

Sequences

Length

1,136

Mass

124,614

Sequence

MFSRKKRELMKTPSISKKNRAGSPSPQPSGELPRKDGADAVFPGPSLEPPAGSSGVKATGTLKRPTSLSRHASAAGFPLSGAASWTLGRSHRSPLTAASPGELPTEGAGPDVVEDISHLLADVARFAEGLEKLKECVLRDDLLEARRPRAHECLGEALRVMHQIISKYPLLNTVETLTAAGTLIAKVKAFHYESNNDLEKQEFEKALETIAVAFSSTVSEFLMGEVDSSTLLAVPPGDSSQSMESLYGPGSEGTPPSLEDCDAGCLPAEEVDVLLQRCEGGVDAALLYAKNMAKYMKDLISYLEKRTTLEMEFAKGLQKIAHNCRQSVMQEPHMPLLSIYSLALEQDLEFGHSMVQAVGTLQTQTFMQPLTLRRLEHEKRRKEIKEAWHRAQRKLQEAESNLRKAKQGYVQRCEDHDKARFLVAKAEEEQAGSAPGAGSTATKTLDKRRRLEEEAKNKAEEAMATYRTCVADAKTQKQELEDTKVTALRQIQEVIRQSDQTIKSATISYYQMMHMQTAPLPVHFQMLCESSKLYDPGQQYASHVRQLQRDQEPDVHYDFEPHVSANAWSPVMRARKSSFNVSDVARPEAAGSPPEEGGCTEGTPAKDHRAGRGHQVHKSWPLSISDSDSGLDPGPGAGDFKKFERTSSSGTMSSTEELVDPDGGAGASAFEQADLNGMTPELPVAVPSGPFRHEGLSKAARTHRLRKLRTPAKCRECNSYVYFQGAECEECCLACHKKCLETLAIQCGHKKLQGRLQLFGQDFSHAARSAPDGVPFIVKKCVCEIERRALRTKGIYRVNGVKTRVEKLCQAFENGKELVELSQASPHDISNVLKLYLRQLPEPLISFRLYHELVGLAKDSLKAEAEAKAASRGRQDGSESEAVAVALAGRLRELLRDLPPENRASLQYLLRHLRRIVEVEQDNKMTPGNLGIVFGPTLLRPRPTEATVSLSSLVDYPHQARVIETLIVHYGLVFEEEPEETPGGQDESSNQRAEVVVQVPYLEAGEAVVYPLQEAAADGCRESRVVSNDSDSDLEEASELLSSSEASALGHLSFLEQQQSEASLEVASGSHSGSEEQLEATAREDGDGDEDGPAQQLSGFNTNQSNNVLQAPLPPMRLRGGRMTLGSCRERQPEFV

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q92619-1; Sequence=Displayed; Name=2; IsoId=Q92619-2; Sequence=VSP_044707

Alternative Sequence

1..30; MFSRKKRELMKTPSISKKNRAGSPSPQPSG -> MSRGQRVLKGLLGWVCTWTWAWRARLGARGCGLHVLCPRDLPLPPE (in isoform 2)

3D Structural Models

3D Structure

X-ray crystallography (2)

Domain & Motif Annotations

Compositional Bias

646..655; Low complexity; 1095..1109; Polar residues

Coiled Coil

376..412; 440..499

Zinc Finger

702..747; Phorbol-ester/DAG-type

Domain (CC)

Rho-GAP domain is able to regulate RhoGTPase activity, actin cytoskeleton and cell spreading. However N-terminally BAR domain plays an autoinhibitory role.

Domain (FT)

269..539; F-BAR; 761..974; Rho-GAP

Region

1..73; Disordered; 91..110; Disordered; 583..662; Disordered; 1061..1136; Disordered

Clinical Relevance1

Antibody (2)

HPA019816 HPA079252

Supporting Publications1

PMID	Title	Abstract
30646616	Preferential Localization of MUC1 Glycoprotein in Exosomes Secreted by Non-Small Cell Lung Carcinoma Cells.	THBS1, ANXA6, HIST1H4A, COL18A1, MDK, SRGN, ENO1, TUBA4A, SLC3A2, GPI, MIF, MUC1, TALDO1, SLC7A5, ICAM1, HSP90AA1, G6PD, and LRP1 were found to be expressed in exosomes at more than 5-fold higher level as compared to total cellular membrane proteins.