Protein detail

SEM4D

Semaphorin-4D (A8) (BB18) (GR3) (CD antigen CD100)

Entry name SEM4D	UniProt ID Q92854	EVMP confidence score 0.50
Supporting publications (n) 1	Transmembrane count 1	Protein classification CD markersPlasma proteinsPredicted intracellular proteinsPredicted membrane proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information13

Protein Names

Semaphorin-4D (A8) (BB18) (GR3) (CD antigen CD100)

Protein Class (4)

CD markersPlasma proteinsPredicted intracellular proteinsPredicted membrane proteins

Protein Function (2)

CD markers
Predicted intracellular proteins

Transmembrane

735..755; Helical

Transmembrane Count

Ensembl

ENSG00000187764

Entrez Gene Symbol

SEMA4D

Gene Synonym (5)

C9orf164CD100coll-4FLJ39737SEMAJ

Gene Description

Semaphorin 4D

Chromosome

Position

89360787-89498130

Supporting publications (n)

EVMP confidence score

0.50

Function & Pathway7

Protein Function (2)

CD markers
Predicted intracellular proteins

Cellular Component (5)

Molecular Function (9)

Biological Process (3)

KEGG

hsa04360 Axon guidance

Reactome (6)

Mediation Categories (3)

Adhesion and uptake mediationClinical-translation mediationReceptor-signaling mediation

Relations & Evidence49

Ligand-Receptor Signaling (42)

42 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
transmembrane_sosui	transmembrane_predicted	Almen2009	No	No	No	Yes	No
transmembrane_tmhmm	transmembrane_predicted	Almen2009	No	No	No	Yes	No

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Regulatory Interaction Network (1)

1 record.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
SEM4D	Q92854	PLXB1	O43157	Yes	Yes	No	iTALKICELLNETSIGNORHINTCellPhoneDB_CellinkerDIPHPMR_talklrHPMRCellChatDBHPRD_LRdbtalklrHPRDIntActRamilowski2015_Baccin2019WangRamilowski2015HPMR_LRdbHPRD_talklrBaccin2019CellinkerSTRING_talklrEMBRACECellTalkDBFantom5_LRdbHPMR_CellinkerconnectomeDB2020LRdb	Cellinker:10520995 CellTalkDB:10520995 ICELLNET:11245885 DIP:20877282 Cellinker:24043639 LRdb:10520995 Cellinker:12198496 connectomeDB2020:10520995 connectomeDB2020:11937491 Baccin2019:10520995 LRdb:11937491 Cellinker:27533782 SIGNOR:12198496 Baccin2019:12198496 HPRD:11937491 HPMR:10520995 HINT:20877282 LRdb:12198496 Baccin2019:11937491 connectomeDB2020:12198496 CellChatDB:27533782 IntAct:20877282 Cellinker:11937491

Protein Complex Composition (5)

5 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
PlexinC1-SEMA7A complex	PLXNB1 SEMA4D	O43157 Q92854	1:1	CompleatCORUMPDB	PDB:3ol2CORUM:5661Compleat:HC2843	10520995
	CHST3 CRLF1 ECEL1 GNPAT IDUA NDST2 NXPE3 SCGB2A2 SEMA3C SEMA4D VCAM1	O15228 O75462 O95672 P19320 P35475 P52849 Q13296 Q7LGC8 Q92854 Q969Y0 Q99985	0:0:0:0:0:0:0:0:0:0:0	hu.MAP2
	ECEL1 SCGB2A2 SEMA3C SEMA4D	O95672 Q13296 Q92854 Q99985	0:0:0:0	hu.MAP2
	SEMA4D VCAM1	P19320 Q92854	0:0	hu.MAP2
	SEMA4D	Q92854	2	PDB	PDB:1olz

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion Chromatography	Mass spectrometryWestern blottingELISA	3	38731868 30550287 40311616

Sequence, Structure & Domains14

Sequences

Length

862

Mass

96,150

Sequence

MRMCTPIRGLLMALAVMFGTAMAFAPIPRITWEHREVHLVQFHEPDIYNYSALLLSEDKDTLYIGAREAVFAVNALNISEKQHEVYWKVSEDKKAKCAEKGKSKQTECLNYIRVLQPLSATSLYVCGTNAFQPACDHLNLTSFKFLGKNEDGKGRCPFDPAHSYTSVMVDGELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPSFVFADVIRKSPDSPDGEDDRVYFFFTEVSVEYEFVFRVLIPRIARVCKGDQGGLRTLQKKWTSFLKARLICSRPDSGLVFNVLRDVFVLRSPGLKVPVFYALFTPQLNNVGLSAVCAYNLSTAEEVFSHGKYMQSTTVEQSHTKWVRYNGPVPKPRPGACIDSEARAANYTSSLNLPDKTLQFVKDHPLMDDSVTPIDNRPRLIKKDVNYTQIVVDRTQALDGTVYDVMFVSTDRGALHKAISLEHAVHIIEETQLFQDFEPVQTLLLSSKKGNRFVYAGSNSGVVQAPLAFCGKHGTCEDCVLARDPYCAWSPPTATCVALHQTESPSRGLIQEMSGDASVCPDKSKGSYRQHFFKHGGTAELKCSQKSNLARVFWKFQNGVLKAESPKYGLMGRKNLLIFNLSEGDSGVYQCLSEERVKNKTVFQVVAKHVLEVKVVPKPVVAPTLSVVQTEGSRIATKVLVASTQGSSPPTPAVQATSSGAITLPPKPAPTGTSCEPKIVINTVPQLHSEKTMYLKSSDNRLLMSLFLFFFVLFLCLFFYNCYKGYLPRQCLKFRSALLIGKKKPKSDFCDREQSLKETLVEPGSFSQQNGEHPKPALDTGYETEQDTITSKVPTDREDSQRIDDLSARDKPFDVKCELKFADSDADGD

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q92854-1; Sequence=Displayed; Name=2; IsoId=Q92854-2; Sequence=VSP_039483, VSP_039484

Alternative Sequence

555..738; DKSKGSYRQHFFKHGGTAELKCSQKSNLARVFWKFQNGVLKAESPKYGLMGRKNLLIFNLSEGDSGVYQCLSEERVKNKTVFQVVAKHVLEVKVVPKPVVAPTLSVVQTEGSRIATKVLVASTQGSSPPTPAVQATSSGAITLPPKPAPTGTSCEPKIVINTVPQLHSEKTMYLKSSDNRLLMS -> ASSPKPLPPPGSSSLSCLGHVGDRRLSSPWTPWPASGAGPDSSSRVSLLPPFLSDQAQHVHALGNFYLFCQATGPADIRFVWEKNGRALETCVPVQTHALPDGRAHALSWLQDAIRESAEYRCSVLSSAGNKTSKVQVAVMRPEVTHQERWTRELSAWRAVAGEHDRMMQSWRKAWESCSKDTL (in isoform 2); 739..862; Missing (in isoform 2)

3D Structural Models

Turn

34..36; 104..107; 129..131; 140..143; 153..155; 203..205; 349..351; 377..379; 524..527; 532..534; 604..607

Helix

91..99; 284..286; 330..339; 373..376; 383..385; 388..396; 407..409; 503..505; 509..514; 539..541; 550..552; 616..618

Beta Strand

29..32; 40..42; 52..55; 59..65; 67..74; 77..86; 111..117; 119..127; 134..139; 164..169; 172..180; 185..191; 193..195; 210..217; 230..238; 249..257; 264..267; 275..279; 292..299; 308..314; 316..318; 320..329; 342..346; 354..357; 399..404; 412..417; 420..429; 431..433; 435..443; 446..453; 455..465; 475..478; 481..484; 486..490; 495..500; 520..523; 528..531; 553..555; 560..567; 572..574; 584..593; 598..602; 609..611; 620..629; 631..647

3D Structure

X-ray crystallography (2)

Domain & Motif Annotations

Compositional Bias

827..837; Basic and acidic residues

Domain (FT)

22..500; Sema; 502..551; PSI; 554..636; Ig-like C2-type

Region

794..837; Disordered

Protein Families

Semaphorin family

Sequence Similarities

Belongs to the semaphorin family.

Clinical Relevance4

Related Diseases (4)

Choreiform disorder Lung cancer Metastatic lymph node neoplasm Multiple sclerosis

Biomarker

Phase 2; Phase 1; Phase 1/2

Drugs (2)

PEPINEMAB NULL

Antibody (2)

HPA015662 HPA023277

Supporting Publications1

PMID	Title	Abstract
27894104	Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.	No abstract available