Protein detail

ARHG1

Rho guanine nucleotide exchange factor 1 (115 kDa guanine nucleotide exchange factor) (p115-RhoGEF) (p115RhoGEF) (Sub1.5)

Entry name ARHG1	UniProt ID Q92888	EVMP confidence score 0.50
Supporting publications (n) 3	Transmembrane count	Protein classification Disease related genesHuman disease related genesPlasma proteinsPredicted intracellular proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

Rho guanine nucleotide exchange factor 1 (115 kDa guanine nucleotide exchange factor) (p115-RhoGEF) (p115RhoGEF) (Sub1.5)

Protein Class (4)

Disease related genesHuman disease related genesPlasma proteinsPredicted intracellular proteins

Protein Function (3)

Human disease related genes:Immune system diseases:Primary immunodeficiency
Disease related genes
Predicted intracellular proteins

Ensembl

ENSG00000076928

Entrez Gene Symbol

ARHGEF1

Gene Synonym (3)

LBCL2P115-RHOGEFSUB1.5

Gene Description

Rho guanine nucleotide exchange factor 1

Chromosome

Position

41883173-41930150

Supporting publications (n)

EVMP confidence score

0.50

Function & Pathway7

Protein Function (3)

Human disease related genes:Immune system diseases:Primary immunodeficiency
Disease related genes
Predicted intracellular proteins

Cellular Component (3)

Molecular Function (5)

Biological Process (3)

KEGG (10)

Reactome (11)

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Mediation Categories

Receptor-signaling mediation

Relations & Evidence18

Enzyme-Mediated Modification (3)

3 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
ARHGEF1	JAK2	O60674	Y	738	phosphorylation	Sparser_ProtMapperSIGNORProtMapperdbPTMSIGNOR_ProtMapperREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper	SIGNOR:20098430 dbPTM:20098430 ProtMapper:20098430 ProtMapper:25981295
ARHGEF1	JAK2	O60674	Y	753	phosphorylation	MIMPPhosphoSite_MIMP
ARHGEF1	PRKCA	P17252	S	240	phosphorylation	PhosphoSite

Ligand-Receptor Signaling (5)

5 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No

Regulatory Interaction Network (4)

4 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
GNA13	Q14344	ARHG1	Q92888	Yes	Yes	No	WangKEGG-MEDICUSSIGNORHPRDCui2007HINTCA1SPIKE_LC	HINT:20300064 CA1:9641916 SIGNOR:9641915 HPRD:12681510 SIGNOR:9641916 SIGNOR:14607242 HINT:15735747 HPRD:9641916 HINT:14634662 HINT:12681510 SPIKE_LC:16189514
KPCA	P17252	ARHG1	Q92888	Yes	Yes	No	PhosphoPointSIGNORProtMapperHPRDPhosphoSitePhosphoSite_ProtMapper	HPRD:12754211 PhosphoSite:32881857 SIGNOR:32881857
JAK2	O60674	ARHG1	Q92888	Yes	Yes	No	PhosphoSite_MIMPMIMPiPTMnetSIGNORProtMapperdbPTMSIGNOR_ProtMapperREACH_ProtMapperPhosphoSitePhosphoSite_ProtMapper	dbPTM:20098430 ProtMapper:20098430 ProtMapper:25981295 SIGNOR:20098430 PhosphoSite:20098430
ARHG1	Q92888	RHOA	P61586	Yes	Yes	No	KEGG-MEDICUSSIGNORCui2007HINTBioGRIDACSNWang	ACSN:9113980 HINT:12748184 ACSN:9789025 HINT:34591642 SIGNOR:32203420 HINT:32203420 SIGNOR:10836144 BioGRID:12748184 ACSN:11149925 ACSN:9641915 HINT:33961781

Protein Complex Composition (5)

5 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
GNA14-p115RhoGEF complex	ARHGEF1 GNA14	O95837 Q92888	1:1	CompleatCORUM	CORUM:3177Compleat:HC2665	15735747
	ARCN1 ARHGEF1 BET1L COPA COPB1 COPB2 COPE COPG1 COPG2 COPZ1 GBF1 GOSR2 RHOB SEC22B UBC	O14579 O14653 O75396 P0CG48 P35606 P48444 P53618 P53621 P61923 P62745 Q92538 Q92888 Q9NYM9 Q9UBF2 Q9Y678	1:1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC9874
	ARHGEF1	Q92888	2	PDB	PDB:3odoPDB:3odwPDB:3p6aPDB:3odx
	ARHGEF17 RASL12 RERG	Q96A58 Q96PE2 Q9NYN1	0:0:0	hu.MAP2
	ARHGEF17 RASL12	Q96PE2 Q9NYN1	0:0	hu.MAP

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential Ultracentrifugation	Mass spectrometry	1	37354366

Sequence, Structure & Domains14

Sequences

Length

912

Mass

102,435

Sequence

MEDFARGAASPGPSRPGLVPVSIIGAEDEDFENELETNSEEQNSQFQSLEQVKRRPAHLMALLQHVALQFEPGPLLCCLHADMLGSLGPKEAKKAFLDFYHSFLEKTAVLRVPVPPNVAFELDRTRADLISEDVQRRFVQEVVQSQQVAVGRQLEDFRSKRLMGMTPWEQELAQLEAWVGRDRASYEARERHVAERLLMHLEEMQHTISTDEEKSAAVVNAIGLYMRHLGVRTKSGDKKSGRNFFRKKVMGNRRSDEPAKTKKGLSSILDAARWNRGEPQVPDFRHLKAEVDAEKPGATDRKGGVGMPSRDRNIGAPGQDTPGVSLHPLSLDSPDREPGADAPLELGDSSPQGPMSLESLAPPESTDEGAETESPEPGDEGEPGRSGLELEPEEPPGWRELVPPDTLHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDLFFQPMAECLFFPLEELQNIFPSLDELIEVHSLFLDRLMKRRQESGYLIEEIGDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQRKDPRFCAFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIGQNTEEPTEREKVELAAECCREILHHVNQAVRDMEDLLRLKDYQRRLDLSHLRQSSDPMLSEFKNLDITKKKLVHEGPLTWRVTKDKAVEVHVLLLDDLLLLLQRQDERLLLKSHSRTLTPTPDGKTMLRPVLRLTSAMTREVATDHKAFYVLFTWDQEAQIYELVAQTVSERKNWCALITETAGSLKVPAPASRPKPRPSPSSTREPLLSSSENGNGGRETSPADARTERILSDLLPFCRPGPEGQLAATALRKVLSLKQLLFPAEEDNGAGPPRDGDGVPGGGPLSPARTQEIQENLLSLEETMKQLEELEEEFCRLRPLLSQLGGNSVPQPGCT

Alternative Products

Event=Alternative splicing; Named isoforms=4; Name=1; IsoId=Q92888-1; Sequence=Displayed; Name=2; IsoId=Q92888-2; Sequence=VSP_008125; Name=3; IsoId=Q92888-3; Sequence=VSP_037766; Name=4; IsoId=Q92888-4; Sequence=VSP_037766, VSP_008125, VSP_057289

Alternative Sequence

1; M -> MASLSTWSSPAEPREM (in isoform 3 and isoform 4); 76..108; Missing (in isoform 2 and isoform 4); 831..912; VLSLKQLLFPAEEDNGAGPPRDGDGVPGGGPLSPARTQEIQENLLSLEETMKQLEELEEEFCRLRPLLSQLGGNSVPQPGCT -> GVGGGILPPETPPVSAWGELCPPAWLHLRFPPRKAFCKKERNGGEDVRDHPHPHSCRSISHPEGLRRGSCGPRLGGAQLGLLAPHEPRPSLPPALCLGDSGLHSGGHHGDPGHLSIACGGHPSTPTPKCLRSVFIP (in isoform 4)

3D Structural Models

Turn

26..33; 127..129; 399..401

Helix

49..52; 56..69; 73..84; 89..103; 116..122; 132..144; 147..162; 169..176; 183..203; 205..207; 212..228; 404..407; 413..441; 443..449; 454..460; 464..484; 493..500; 502..517; 519..532; 534..544; 547..549; 554..557; 560..577; 582..621; 625..629; 633..635; 710..712; 745..760

Beta Strand

18..20; 636..638; 644..646; 648..661; 663..681; 684..686; 694..697; 706..709; 713..717; 719..721; 724..729; 737..741

3D Structure

X-ray crystallography (7)

Domain & Motif Annotations

Compositional Bias

283..313; Basic and acidic residues; 365..381; Acidic residues; 777..789; Low complexity

Coiled Coil

865..896

Domain (CC)

The RGSL domain, also known as rgRGS domain, is necessary but not sufficient for GAP activity.; DOMAIN: The DH domain is involved in interaction with CCPG1..

Domain (FT)

41..232; RGSL; 416..605; DH; 647..760; PH

Region

248..413; Disordered; 763..802; Disordered; 841..865; Disordered

Clinical Relevance4

Antibody (3)

CAB009502 HPA012924 HPA060784

Interaction Protein (2)

ENSG00000120063ENSG00000143878

Interaction Count

Interaction Dataset

intact_biogrid

Supporting Publications3

PMID	Title	Abstract
37457308	Platelet-derived extracellular vesicles formulated with hyaluronic acid gels for application at the bone-implant interface: An animal study.	No abstract available
37516879	Exosomes promote hFOB1.19 proliferation and differentiation via LINC00520.	After human osteoblasts hFOB1.19 were exposed to the obtained exosomes, cell survival, cell cycle, apoptosis and calcium deposits of hFOB1.19 cell were detected by MTT, 7-aminoactinomycin D, Annexin V-FITC/propidium iodide and Alizarin red staining, respectively.
39155501	The efficacy of adipose stem cell-derived exosomes in hair regeneration based on a preclinical and clinical study.	ASC-Exosomes impact hDPCs, increasing proliferation and the upregulation of hair growth-related genes, including ALP, VCAN, β-catenin, and LEF-1. First, using human hair follicle (HF) dermal papilla cells (hDPCs) treated with ASC-Exosomes, ALP, VCAN, β-catenin, and LEF-1 levels with RT-PCR and p-GSK3β, GSK3β, β-catenin, ALP, and β-actin levels with western blot analysis were assessed.