Protein detail

M4K1

Mitogen-activated protein kinase kinase kinase kinase 1 (EC 2.7.11.1) (Hematopoietic progenitor kinase) (MAPK/ERK kinase kinase kinase 1) (MEK kinase kinase 1) (MEKKK 1)

Protein symbol M4K1	UniProt ID Q92918	EVMP score 0.38
Frequency 1	Transmembrane count	Protein classification EnzymesPredicted intracellular proteins

EVMP score: annotation confidence score.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information

Protein Names

Mitogen-activated protein kinase kinase kinase kinase 1 (EC 2.7.11.1) (Hematopoietic progenitor kinase) (MAPK/ERK kinase kinase kinase 1) (MEK kinase kinase 1) (MEKKK 1)

Protein Class

EnzymesPredicted intracellular proteins

Protein Function

ENZYME proteins:Transferases
Enzymes
Kinases:STE Ser/Thr protein kinases
Predicted intracellular proteins

Ensembl

ENSG00000104814

Entrez Gene Symbol

MAP4K1

Gene Synonym

HPK1

Gene Description

Mitogen-activated protein kinase kinase kinase kinase 1

Chromosome

Position

38587641-38618882

Frequency

EVMP Score

0.38

Fluorescence & Localization

Tissue SpecifictestisCell SpecificEarly spermatidsSingle-Nuclei Brain Specificcommitted oligodendrocyte precursorSecretome LocationSecreted in male reproductive systemSecretome FunctionNo annotated function

Function & Pathway

Protein Function

ENZYME proteins:Transferases
Enzymes
Kinases:STE Ser/Thr protein kinases
Predicted intracellular proteins

Cellular Component

Molecular Function

Biological Process

KEGG

hsa04010 MAPK signaling pathway

Mediation Categories

Clinical-translation mediationReceptor-signaling mediation

Relations & Evidence

Enzyme-Mediated Modification

13 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
MAP4K1	PDK1	Q15118	S	171	phosphorylation	KEA	KEA:15743830
MAP4K1	PRKCA	P17252	S	366	phosphorylation	KEA	KEA:17570479
MAP4K1	RPS6KA3	P51812	S	368	phosphorylation	KEA	KEA:17570479

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Ligand-Receptor Signaling

6 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
receptor	receptor	OmniPath	No	Yes	No	No	No
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
receptor	receptor	scConnect	No	Yes	No	No	No

Regulatory Interaction Network

15 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
M4K1	Q92918	CAR11	Q9BXL7	Yes	Yes	No	PhosphoSite_MIMPMIMPNetPathPhosphoSite_norefSIGNORiPTMnetProtMapperSPIKE_LCREACH_ProtMapperSPIKEPhosphoSite_ProtMapper	SIGNOR:19706536 SPIKE:19706536 NetPath:19706536 ProtMapper:33603723 SPIKE_LC:19706536
CRKL	P46109	M4K1	Q92918	Yes	Yes	No	WangNetPathSIGNORHPRDHINTSPIKE_LCSPIKE	HINT:9788432 SPIKE_LC:11279207 SPIKE_LC:17145710 HPRD:11279207 SPIKE:17145710 SPIKE:11279207 SIGNOR:9891069 NetPath:11279207 HINT:9891069 HPRD:9891069
CRK	P46108	M4K1	Q92918	Yes	Yes	No	DOMINOWangNetPathSIGNORHPRDCui2007HINTSPIKE_LCLit-BM-17SPIKE	HINT:9788432 SPIKE:16713569 Lit-BM-17:17474147 SPIKE_LC:11279207 Lit-BM-17:11279207 HINT:9891069 SPIKE_LC:17474147 Lit-BM-17:9891069 SPIKE:11279207 SPIKE:17474147 SIGNOR:9891069 SPIKE_LC:16713569 HPRD:11432831 NetPath:11279207 DOMINO:17474147 Lit-BM-17:9788432 HPRD:9891069
GRB2	P62993	M4K1	Q92918	Yes	Yes	No	DOMINOWangNetPathSIGNORHPRDHINTIntActSPIKE_LCLit-BM-17SPIKE	Lit-BM-17:17474147 HPRD:9346925 SPIKE:11279207 SPIKE:17474147 IntAct:9346925 HINT:32707033 HPRD:9891069 Lit-BM-17:9891069 Lit-BM-17:9346925 NetPath:11279207 HINT:9891069 Lit-BM-17:9788432 SPIKE_LC:11279207 Lit-BM-17:11279207 NetPath:9346925 SPIKE_LC:17474147 HPRD:9788432 SIGNOR:9891069 HINT:9788432 HINT:9346925 IntAct:17474147 DOMINO:17474147
M4K1	Q92918	M3K7	O43318	Yes	Yes	No	WangNetPathPhosphoPointHPRDSignaLink3InnateDBSPIKE_LCSPIKE	InnateDB:10224067 HPRD:10224067 NetPath:10224067 SignaLink3:10224067 SPIKE:10224067 SignaLink3:23331499 SPIKE_LC:10224067
M4K1	Q92918	M3K11	Q16584	Yes	Yes	No	HPRD_MIMPSIGNORProtMapperRLIMS-P_ProtMapperHINTPhosphoSite_KEASignaLink3dbPTMphosphoELM_KEALit-BM-17HPRDKinexus_KEAWangPhosphoSite_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPPhosphoSite_norefPhosphoPointiPTMnetKEAHPRD_KEAphosphoELMSIGNOR_ProtMapper	HINT:11053428 SignaLink3:18988627 KEA:11053428 SignaLink3:11053428 phosphoELM:11053428 HPRD:11053428 SIGNOR:11053428 HINT:9346925 SignaLink3:9003777 ProtMapper:11053428 Lit-BM-17:9346925 SignaLink3:23331499 Lit-BM-17:11053428 dbPTM:11053428
M4K1	Q92918	M3K1	Q13233	Yes	Yes	No	InnateDBWangSIGNORSignaLink3	SignaLink3:9003777 SIGNOR:8824585 InnateDB:8824585 SignaLink3:23331499
JIP1	Q9UQF2	M4K1	Q92918	Yes	Yes	No	WangSignaLink3	SignaLink3:9868371 SignaLink3:23331499
KSYK	P43405	M4K1	Q92918	Yes	Yes	No	HPRD_MIMPSIGNORProtMapperPhosphoSite_KEAphosphoELM_KEALi2012PhosphoNetworksHPRDKinexus_KEAWangphosphoELM_MIMPPhosphoSite_MIMPMIMPiPTMnetPhosphoPointKEAHPRD_KEAphosphoELMSIGNOR_ProtMapper	SIGNOR:11514608 KEA:11487585 ProtMapper:11514608 phosphoELM:11514608 KEA:17192257 KEA:11514608 HPRD:11514608
M4K1	Q92918	PSMD2	Q13200	Yes	Yes	No	SIGNORPhosphoSite_ProtMapperProtMapper	SIGNOR:31843888

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Protein Complex Composition

0 records.

Isolation & Detection Technology

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion Chromatography	Mass spectrometry	5	36982991 37499326 37531777 37612209 38902710

Sequence, Structure & Domains

Sequences

Length

833

Mass

91,296

Sequence

MDVVDPDIFNRDPRDHYDLLQRLGGGTYGEVFKARDKVSGDLVALKMVKMEPDDDVSTLQKEILILKTCRHANIVAYHGSYLWLQKLWICMEFCGAGSLQDIYQVTGSLSELQISYVCREVLQGLAYLHSQKKIHRDIKGANILINDAGEVRLADFGISAQIGATLARRLSFIGTPYWMAPEVAAVALKGGYNELCDIWSLGITAIELAELQPPLFDVHPLRVLFLMTKSGYQPPRLKEKGKWSAAFHNFIKVTLTKSPKKRPSATKMLSHQLVSQPGLNRGLILDLLDKLKNPGKGPSIGDIEDEEPELPPAIPRRIRSTHRSSSLGIPDADCCRRHMEFRKLRGMETRPPANTARLQPPRDLRSSSPRKQLSESSDDDYDDVDIPTPAEDTPPPLPPKPKFRSPSDEGPGSMGDDGQLSPGVLVRCASGPPPNSPRPGPPPSTSSPHLTAHSEPSLWNPPSRELDKPPLLPPKKEKMKRKGCALLVKLFNGCPLRIHSTAAWTHPSTKDQHLLLGAEEGIFILNRNDQEATLEMLFPSRTTWVYSINNVLMSLSGKTPHLYSHSILGLLERKETRAGNPIAHISPHRLLARKNMVSTKIQDTKGCRACCVAEGASSGGPFLCGALETSVVLLQWYQPMNKFLLVRQVLFPLPTPLSVFALLTGPGSELPAVCIGVSPGRPGKSVLFHTVRFGALSCWLGEMSTEHRGPVQVTQVEEDMVMVLMDGSVKLVTPEGSPVRGLRTPEIPMTEAVEAVAMVGGQLQAFWKHGVQVWALGSDQLLQELRDPTLTFRLLGSPRLECSGTISPHCNLLLPGSSNSPASASRVAGITGL

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q92918-1; Sequence=Displayed; Name=2; IsoId=Q92918-2; Sequence=VSP_040340

Alternative Sequence

800..833; LECSGTISPHCNLLLPGSSNSPASASRVAGITGL -> PVVVETRPVDDPTAPSNLYIQE (in isoform 2)

3D Structural Models

Turn

37..39; 214..217; 240..242; 557..560; 682..684; 776..778

Helix

13..15; 57..67; 99..106; 111..130; 140..142; 157..171; 176..178; 181..189; 196..210; 220..227; 245..254; 259..261; 265..268; 272..275; 282..292; 567..572; 638..640

Beta Strand

9..11; 17..26; 29..36; 42..49; 52..54; 77..83; 86..92; 143..145; 151..153; 296..298; 488..494; 498..505; 512..518; 521..528; 531..538; 542..548; 551..556; 561..566; 598..600; 609..613; 622..626; 628..637; 642..650; 661..663; 672..679; 686..691; 707..709; 712..717; 720..725; 728..732; 741..743; 746..748; 753..758; 760..767; 770..774; 784..786; 790..796

3D Structure

X-ray crystallography (40)

Domain & Motif Annotations

Compositional Bias

333..348; Basic and acidic residues; 366..375; Polar residues; 376..385; Acidic residues; 431..445; Pro residues

Domain (FT)

17..274; Protein kinase; 495..800; CNH

Region

296..478; Disordered

Protein Families

Protein kinase superfamily
STE Ser/Thr protein kinase family
STE20 subfamily

Sequence Similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Clinical Relevance

Related Diseases

Solid tumour/cancer

Biomarker

Phase 1/2

Antibody

HPA007419 CAB007754

Interaction Protein

ENSG00000096384ENSG00000099942ENSG00000146648ENSG00000158092ENSG00000167193ENSG00000177885

Interaction Count

Interaction Dataset

intact_biogrid