Protein detail

M4K1

Mitogen-activated protein kinase kinase kinase kinase 1 (EC 2.7.11.1) (Hematopoietic progenitor kinase) (MAPK/ERK kinase kinase kinase 1) (MEK kinase kinase 1) (MEKKK 1)

Protein symbol M4K1	UniProt ID Q92918	EVMP score 0.38
Frequency 1	Transmembrane count	Protein classification EnzymesPredicted intracellular proteins

EVMP score: annotation confidence score.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information

Protein Names

Mitogen-activated protein kinase kinase kinase kinase 1 (EC 2.7.11.1) (Hematopoietic progenitor kinase) (MAPK/ERK kinase kinase kinase 1) (MEK kinase kinase 1) (MEKKK 1)

Protein Class

EnzymesPredicted intracellular proteins

Protein Function

ENZYME proteins:Transferases
Enzymes
Kinases:STE Ser/Thr protein kinases
Predicted intracellular proteins

Ensembl

ENSG00000104814

Entrez Gene Symbol

MAP4K1

Gene Synonym

HPK1

Gene Description

Mitogen-activated protein kinase kinase kinase kinase 1

Chromosome

Position

38587641-38618882

Frequency

EVMP Score

0.38

Fluorescence & Localization

Tissue SpecifictestisCell SpecificEarly spermatidsSingle-Nuclei Brain Specificcommitted oligodendrocyte precursorSecretome LocationSecreted in male reproductive systemSecretome FunctionNo annotated function

Function & Pathway

Protein Function

ENZYME proteins:Transferases
Enzymes
Kinases:STE Ser/Thr protein kinases
Predicted intracellular proteins

Cellular Component

Molecular Function

Biological Process

KEGG

hsa04010 MAPK signaling pathway

Mediation Categories

Clinical-translation mediationReceptor-signaling mediation

Relations & Evidence

Enzyme-Mediated Modification

13 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
MAP4K1	SYK	P43405	Y	381	phosphorylation	PhosphoNetworksphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperKEAphosphoELMLi2012SIGNOR_ProtMapper	SIGNOR:11514608 KEA:11487585 ProtMapper:11514608 phosphoELM:11514608 KEA:17192257 KEA:11514608
MAP4K1	LYN	P07948	Y	381	phosphorylation	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperKEASIGNOR_ProtMapper	SIGNOR:11514608 KEA:11487585 ProtMapper:11514608 KEA:17570479 KEA:11514608
MAP4K1	ABL1	P00519	Y	232	phosphorylation	MIMPHPRD_MIMPSIGNORProtMapperHPRDKEASIGNOR_ProtMapper	SIGNOR:11278340 ProtMapper:11278340 KEA:11278340 HPRD:11278340
MAP4K1	INSR	P06213	Y	381	phosphorylation	Li2012KEA	KEA:17570479
MAP4K1	PDGFRB	P09619	Y	381	phosphorylation	Li2012
MAP4K1	SRC	P12931	Y	381	phosphorylation	Li2012
MAP4K1	PRKD1	Q15139	S	171	phosphorylation	phosphoELM_MIMPPhosphoSite_MIMPMIMPProtMapperPhosphoSitePhosphoSite_ProtMapper
MAP4K1	BCR	P11274	Y	381	phosphorylation	REACH_ProtMapperProtMapper	ProtMapper:11514608
MAP4K1	CDK2	P24941	S	368	phosphorylation	KEA	KEA:17570479
MAP4K1	CSF1R	P07333	Y	381	phosphorylation	KEA	KEA:17570479

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Ligand-Receptor Signaling

6 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
receptor	receptor	OmniPath	No	Yes	No	No	No
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
receptor	receptor	scConnect	No	Yes	No	No	No

Regulatory Interaction Network

15 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
M4K1	Q92918	LCP2	Q13094	Yes	Yes	Yes	WangSparser_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPPhosphoSite_norefPhosphoPointSIGNORProtMapperiPTMnetHPRDRLIMS-P_ProtMapperKEAphosphoELM_KEAphosphoELMSIGNOR_ProtMapperPhosphoSite_ProtMapper	KEA:17353368 ProtMapper:17353368 ProtMapper:28107427 SIGNOR:17353368 ProtMapper:21949020 phosphoELM:17353368 ProtMapper:22105350 HPRD:11487585 ProtMapper:33732413
M4K1	Q92918	GRAP2	O75791	Yes	No	Yes	NetPathPhosphoPointSIGNORELMHPRDWangLit-BM-17	NetPath:11313918 HPRD:15100220 Lit-BM-17:11313918 HPRD:11313918 Lit-BM-17:12496419 SIGNOR:22105350 ELM:15100220
ABL1	P00519	M4K1	Q92918	Yes	Yes	No	DOMINOMIMPHPRD_MIMPiPTMnetPhosphoPointSIGNORProtMapperHPRDHINTPhosphoSite_KEAKEAHPRD_KEASIGNOR_ProtMapperLit-BM-17HPRD-phosSPIKE_LC	ProtMapper:11278340 Lit-BM-17:17474147 SIGNOR:11278340 HINT:9346925 HPRD:11278340 SPIKE_LC:17474147 HPRD-phos:11278340 Lit-BM-17:9346925 KEA:11278340 DOMINO:17474147
LYN	P07948	M4K1	Q92918	Yes	Yes	No	phosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetPhosphoPointSIGNORProtMapperHPRDPhosphoSite_KEAKEAHPRD_KEASIGNOR_ProtMapperNetworKIN_KEA	SIGNOR:11514608 KEA:11487585 ProtMapper:11514608 KEA:17570479 KEA:11514608 HPRD:11514608
KPCD1	Q15139	M4K1	Q92918	Yes	No	No	phosphoELM_MIMPPhosphoSite_MIMPMIMPiPTMnetProtMapperPhosphoSitePhosphoSite_ProtMapper	PhosphoSite:24362026 PhosphoSite:15743830

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Protein Complex Composition

0 records.

Isolation & Detection Technology

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion Chromatography	Mass spectrometry	5	36982991 37499326 37531777 37612209 38902710

Sequence, Structure & Domains

Sequences

Length

833

Mass

91,296

Sequence

MDVVDPDIFNRDPRDHYDLLQRLGGGTYGEVFKARDKVSGDLVALKMVKMEPDDDVSTLQKEILILKTCRHANIVAYHGSYLWLQKLWICMEFCGAGSLQDIYQVTGSLSELQISYVCREVLQGLAYLHSQKKIHRDIKGANILINDAGEVRLADFGISAQIGATLARRLSFIGTPYWMAPEVAAVALKGGYNELCDIWSLGITAIELAELQPPLFDVHPLRVLFLMTKSGYQPPRLKEKGKWSAAFHNFIKVTLTKSPKKRPSATKMLSHQLVSQPGLNRGLILDLLDKLKNPGKGPSIGDIEDEEPELPPAIPRRIRSTHRSSSLGIPDADCCRRHMEFRKLRGMETRPPANTARLQPPRDLRSSSPRKQLSESSDDDYDDVDIPTPAEDTPPPLPPKPKFRSPSDEGPGSMGDDGQLSPGVLVRCASGPPPNSPRPGPPPSTSSPHLTAHSEPSLWNPPSRELDKPPLLPPKKEKMKRKGCALLVKLFNGCPLRIHSTAAWTHPSTKDQHLLLGAEEGIFILNRNDQEATLEMLFPSRTTWVYSINNVLMSLSGKTPHLYSHSILGLLERKETRAGNPIAHISPHRLLARKNMVSTKIQDTKGCRACCVAEGASSGGPFLCGALETSVVLLQWYQPMNKFLLVRQVLFPLPTPLSVFALLTGPGSELPAVCIGVSPGRPGKSVLFHTVRFGALSCWLGEMSTEHRGPVQVTQVEEDMVMVLMDGSVKLVTPEGSPVRGLRTPEIPMTEAVEAVAMVGGQLQAFWKHGVQVWALGSDQLLQELRDPTLTFRLLGSPRLECSGTISPHCNLLLPGSSNSPASASRVAGITGL

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q92918-1; Sequence=Displayed; Name=2; IsoId=Q92918-2; Sequence=VSP_040340

Alternative Sequence

800..833; LECSGTISPHCNLLLPGSSNSPASASRVAGITGL -> PVVVETRPVDDPTAPSNLYIQE (in isoform 2)

3D Structural Models

Turn

37..39; 214..217; 240..242; 557..560; 682..684; 776..778

Helix

13..15; 57..67; 99..106; 111..130; 140..142; 157..171; 176..178; 181..189; 196..210; 220..227; 245..254; 259..261; 265..268; 272..275; 282..292; 567..572; 638..640

Beta Strand

9..11; 17..26; 29..36; 42..49; 52..54; 77..83; 86..92; 143..145; 151..153; 296..298; 488..494; 498..505; 512..518; 521..528; 531..538; 542..548; 551..556; 561..566; 598..600; 609..613; 622..626; 628..637; 642..650; 661..663; 672..679; 686..691; 707..709; 712..717; 720..725; 728..732; 741..743; 746..748; 753..758; 760..767; 770..774; 784..786; 790..796

3D Structure

X-ray crystallography (40)

Domain & Motif Annotations

Compositional Bias

333..348; Basic and acidic residues; 366..375; Polar residues; 376..385; Acidic residues; 431..445; Pro residues

Domain (FT)

17..274; Protein kinase; 495..800; CNH

Region

296..478; Disordered

Protein Families

Protein kinase superfamily
STE Ser/Thr protein kinase family
STE20 subfamily

Sequence Similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Clinical Relevance

Related Diseases

Solid tumour/cancer

Biomarker

Phase 1/2

Antibody

HPA007419 CAB007754

Interaction Protein

ENSG00000096384ENSG00000099942ENSG00000146648ENSG00000158092ENSG00000167193ENSG00000177885

Interaction Count

Interaction Dataset

intact_biogrid