Protein detail

NKD2

Protein naked cuticle homolog 2 (Naked-2) (hNkd2)

Entry name NKD2	UniProt ID Q969F2	EVMP confidence score 0.38
Supporting publications (n)	Transmembrane count	Protein classification Predicted intracellular proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information10

Protein Names

Protein naked cuticle homolog 2 (Naked-2) (hNkd2)

Protein Class

Predicted intracellular proteins

Protein Function

Predicted intracellular proteins

Ensembl

ENSG00000145506

Entrez Gene Symbol

NKD2

Gene Synonym

Naked2

Gene Description

NKD inhibitor of WNT signaling pathway 2

Chromosome

Position

1008802-1038943

EVMP confidence score

0.38

Fluorescence & Localization4

Tissue Specificparathyroid glandCell SpecificMacrophagesSecretome LocationSecreted to bloodSecretome FunctionGrowth factor

Function & Pathway6

Protein Function

Predicted intracellular proteins

Cellular Component (7)

Molecular Function (7)

Biological Process (3)

KEGG (2)

Mediation Categories (3)

Fusion and delivery mediationMetabolism mediationReceptor-signaling mediation

Relations & Evidence15

Enzyme-Mediated Modification (2)

2 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
NKD2	PRKCQ	Q04759	S	31	phosphorylation	PhosphoSite
NKD2	PRKACA	P17612	S	223	phosphorylation	PhosphoSite

Ligand-Receptor Signaling (8)

8 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
ecm	ecm	OmniPath	Yes	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
plasma_membrane	plasma_membrane	UniProt_location	No	No	No	No	No
plasma_membrane	plasma_membrane	OmniPath	No	No	No	No	No
ecm	ecm	Cellinker	Yes	No	No	No	No

Regulatory Interaction Network (4)

4 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
RNF25	Q96BH1	NKD2	Q969F2	Yes	No	Yes	SIGNOR	SIGNOR:18757723
TGFA	P01135	NKD2	Q969F2	Yes	Yes	No	CellinkerHINTSIGNORHPRD	HINT:15064403 HINT:18757723 Cellinker:27635238 SIGNOR:18757723 HPRD:15064403
KAPCA	P17612	NKD2	Q969F2	Yes	No	No	PhosphoSitePhosphoSite_ProtMapperProtMapper	PhosphoSite:30941853
KPCT	Q04759	NKD2	Q969F2	Yes	No	No	PhosphoSitePhosphoSite_ProtMapperProtMapper	PhosphoSite:34433025

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential Ultracentrifugation	Western blottingMass spectrometry	1	34185707

Sequence, Structure & Domains11

Sequences

Length

451

Mass

50,055

Sequence

MGKLQSKHAAAARKRRESPEGDSFVASAYASGRKGAEEAERRARDKQELPNGDPKEGPFREDQCPLQVALPAEKAEGREHPGQLLSADDGERAANREGPRGPGGQRLNIDALQCDVSVEEDDRQEWTFTLYDFDNCGKVTREDMSSLMHTIYEVVDASVNHSSGSSKTLRVKLTVSPEPSSKRKEGPPAGQDREPTRCRMEGELAEEPRVADRRLSAHVRRPSTDPQPCSERGPYCVDENTERRNHYLDLAGIENYTSRFGPGSPPVQAKQEPQGRASHLQARSRSQEPDTHAVHHRRSQVLVEHVVPASEPAARALDTQPRPKGPEKQFLKSPKGSGKPPGVPASSKSGKAFSYYLPAVLPPQAPQDGHHLPQPPPPPYGHKRYRQKGREGHSPLKAPHAQPATVEHEVVRDLPPTPAGEGYAVPVIQRHEHHHHHEHHHHHHHHHFHPS

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q969F2-1; Sequence=Displayed; Name=2; IsoId=Q969F2-2; Sequence=VSP_027900, VSP_027901

Alternative Sequence

265..311; PPVQAKQEPQGRASHLQARSRSQEPDTHAVHHRRSQVLVEHVVPASE -> QLCEKRSSAPRTHSGDKARGVGLCRELWSQAGHPQWPGPFPSGLVAV (in isoform 2); 312..451; Missing (in isoform 2)

Domain & Motif Annotations

Compositional Bias

34..63; Basic and acidic residues; 89..99; Basic and acidic residues; 180..215; Basic and acidic residues; 332..351; Low complexity

Domain (CC)

The N-terminal domain comprising the first 217 amino acid residues is mostly unstructured.

Domain (FT)

119..154; EF-hand

Region

1..108; Disordered; 2..173; Targeting to the basolateral cell membrane; 113..178; Interaction with DVL1, DVL2 and DVL3; 162..237; Disordered; 256..408; Disordered; 300..385; Interaction with TGFA

Protein Families

NKD family

Sequence Similarities

Belongs to the NKD family.

Clinical Relevance4

Antibody

HPA049463

Interaction Protein (3)

ENSG00000108953ENSG00000163743ENSG00000164924

Interaction Count

Interaction Dataset (2)

biogrid_opencellintact_biogrid