Protein detail

AP2M1

AP-2 complex subunit mu (AP-2 mu chain) (Adaptin-mu2) (Adaptor protein complex AP-2 subunit mu) (Adaptor-related protein complex 2 subunit mu) (Clathrin assembly protein complex 2 mu medium chain) (Clathrin coat assembly protein AP50) (Clathrin coat-associated protein AP50) (HA2 50 kDa subunit) (Plasma membrane adaptor AP-2 50 kDa protein)

Entry name AP2M1	UniProt ID Q96CW1	EVMP confidence score 0.50
Supporting publications (n) 2	Transmembrane count	Protein classification Disease related genesEssential proteinsHuman disease related genesPlasma proteinsPotential drug targetsPredicted intracellular proteinsTransporters

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

Protein Class (7)

Disease related genesEssential proteinsHuman disease related genesPlasma proteinsPotential drug targetsPredicted intracellular proteinsTransporters

Protein Function (5)

Transporters
Human disease related genes:Other diseases:Mental and behavioural disorders
Predicted intracellular proteins
Potential drug targets
Disease related genes

Ensembl

ENSG00000161203

Entrez Gene Symbol

AP2M1

Gene Synonym (3)

AP50CLAPM1mu2

Gene Description

Adaptor related protein complex 2 subunit mu 1

Chromosome

Position

184174689-184184214

Supporting publications (n)

EVMP confidence score

0.50

Fluorescence & Localization5

Tissue Specificlymphoid tissueCell SpecificcDCBlood Cell Specificmyeloid DCBlood Lineage Specificdendritic cells

Function & Pathway8

Protein Function (5)

Transporters
Human disease related genes:Other diseases:Mental and behavioural disorders
Predicted intracellular proteins
Potential drug targets
Disease related genes

Cellular Component (16)

Page 1 of 2

Molecular Function (7)

Biological Process (3)

KEGG (5)

Reactome (43)

Page 1 of 5

Canonical Pathways (3)

M165 Pid syndecan 4 pathway
M232 Pid ecadherin stabilization pathway
M71 Pid ilk pathway

Mediation Categories (5)

Adhesion and uptake mediationClinical-translation mediationFusion and delivery mediationImmune mediationReceptor-signaling mediation

Relations & Evidence27

Enzyme-Mediated Modification (4)

4 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
AP2M1	AAK1	Q2M2I8	T	156	phosphorylation	SIGNORProtMapperphosphoELMSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	ProtMapper:11877461 SIGNOR:11877461 phosphoELM:11877457 phosphoELM:11877461
AP2M1	AAK1	Q2M2I8	T	154	phosphorylation	MIMPHPRD_MIMPphosphoELM_MIMPPhosphoSite_MIMP
AP2M1	BMP2K	Q9NSY1	T	156	phosphorylation	PhosphoSite
AP2M1	LRRK2	Q5S007	T	156	phosphorylation	PhosphoSite

Ligand-Receptor Signaling (6)

6 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
plasma_membrane	plasma_membrane	UniProt_location	No	No	No	No	No
plasma_membrane	plasma_membrane	OmniPath	No	No	No	No	No

Regulatory Interaction Network (6)

6 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
AP2M1	Q96CW1	NCKX2	Q9UI40	Yes	No	Yes	SIGNOR	SIGNOR:23431067
LRRK2	Q5S007	AP2M1	Q96CW1	Yes	Yes	No	SIGNORSparser_ProtMapperProtMapper	ProtMapper:34315807 SIGNOR:34315807
AP2M1	Q96CW1	EGFR	P00533	Yes	No	Yes	Lit-BM-17SIGNORIntAct	SIGNOR:19351721 IntAct:31980649 IntAct:20029029 Lit-BM-17:23956138 Lit-BM-17:24797263 Lit-BM-17:20029029 Lit-BM-17:24189400
AAK1	Q2M2I8	AP2M1	Q96CW1	Yes	Yes	No	Sparser_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetSIGNORProtMapperELMHINTphosphoELMSIGNOR_ProtMapperPhosphoSite_ProtMapper	SIGNOR:11877461 ProtMapper:34315807 ProtMapper:31905947 phosphoELM:11877457 ELM:11877457 ELM:34315807 ELM:33137362 ProtMapper:11877461 phosphoELM:11877461 HINT:25852190 HINT:33961781
RN111	Q6ZNA4	AP2M1	Q96CW1	Yes	Yes	No	HuRISIGNOR	SIGNOR:20965945
BMP2K	Q9NSY1	AP2M1	Q96CW1	Yes	No	No	REACH_ProtMapperPhosphoSitePhosphoSite_ProtMapperProtMapper	PhosphoSite:18158287 PhosphoSite:11877457 ProtMapper:32792513 PhosphoSite:33137362 PhosphoSite:16498080

Protein Complex Composition (10)

10 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
AP-1 adaptor complex	AP1B1 AP1G1 AP1G2 AP1M1 AP1M2 AP1S1 AP1S2 AP1S3 AP2B1 AP2M1	O43747 O75843 P56377 P61966 P63010 Q10567 Q96CW1 Q96PC3 Q9BXS5 Q9Y6Q5	1:1:1:1:1:1:1:1:1:1	Compleat	Compleat:HC679	10564262
HT_SC_Cluster66	AP1B1 AP1G1 AP1G2 AP1M1 AP1M2 AP1S1 AP1S2 AP1S3 AP2B1 AP2M1 TMCO4	O43747 O75843 P56377 P61966 P63010 Q10567 Q5TGY1 Q96CW1 Q96PC3 Q9BXS5 Q9Y6Q5	1:1:1:1:1:1:1:1:1:1:1	Compleat	Compleat:HC259
Stargazin-Glu1-mu2 complex	AP2M1 CACNG2 GRIA1	P42261 Q96CW1 Q9Y698	0:0:0	CORUM	CORUM:6196	24217640
	AP1B1 AP1G1 AP1M1 AP1M2 AP1S1 AP2B1 AP2M1 CLINT1 CLTB CLTC GAK GGA2 TGOLN2 UBC	O14976 O43493 O43747 P09497 P0CG48 P61966 P63010 Q00610 Q10567 Q14677 Q96CW1 Q9BXS5 Q9UJY4 Q9Y6Q5	1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC7816
	AP1B1 AP1G1 AP1M1 AP1M2 AP1S1 AP1S2 AP2B1 AP2M1 CLINT1 CLTC GAK GGA3 RAP2B RUNDC3A UBC	O14976 O43747 P0CG48 P56377 P61225 P61966 P63010 Q00610 Q10567 Q14677 Q59EK9 Q96CW1 Q9BXS5 Q9NZ52 Q9Y6Q5	1:1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC5973
	AP2M1 GULP1 KIF13A MAGED4 PJA2 REPS2 REXO1 SNX29 SYNJ1	O43164 O43426 Q8N1G1 Q8NFH8 Q8TEQ0 Q96CW1 Q96JG8 Q9H1H9 Q9UBP9	0:0:0:0:0:0:0:0:0	hu.MAP
	AP2B1 AP2M1 GRB2 TGOLN2 UBC	O43493 P0CG48 P62993 P63010 Q96CW1	1:1:1:1:1	CompleatCFinder	Compleat:HC7560
	AP2A2 AP2B1 AP2M1 SHC1 UBC	O94973 P0CG48 P29353 P63010 Q96CW1	1:1:1:1:1	CompleatCFinder	Compleat:HC9774
	AP2M1 CTLA4	P16410 Q96CW1	1:1	PDB	PDB:1h6e
	AP2M1 IRS1	P35568 Q96CW1	1:1	PDB	PDB:6bnt

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion Chromatography	ELISA	1	26775013

Sequence, Structure & Domains12

Sequences

Length

435

Mass

49,655

Sequence

MIGGLFIYNHKGEVLISRVYRDDIGRNAVDAFRVNVIHARQQVRSPVTNIARTSFFHVKRSNIWLAAVTKQNVNAAMVFEFLYKMCDVMAAYFGKISEENIKNNFVLIYELLDEILDFGYPQNSETGALKTFITQQGIKSQHQTKEEQSQITSQVTGQIGWRREGIKYRRNELFLDVLESVNLLMSPQGQVLSAHVSGRVVMKSYLSGMPECKFGMNDKIVIEKQGKGTADETSKSGKQSIAIDDCTFHQCVRLSKFDSERSISFIPPDGEFELMRYRTTKDIILPFRVIPLVREVGRTKLEVKVVIKSNFKPSLLAQKIEVRIPTPLNTSGVQVICMKGKAKYKASENAIVWKIKRMAGMKESQISAEIELLPTNDKKKWARPPISMNFEVPFAPSGLKVRYLKVFEPKLNYSDHDVIKWVRYIGRSGIYETRC

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q96CW1-1; Sequence=Displayed; Name=2; IsoId=Q96CW1-2; Sequence=VSP_034599

Alternative Sequence

141..142; Missing (in isoform 2)

3D Structural Models

Turn

297..299; 346..349

Helix

28..35; 75..93; 98..103; 106..115; 415..417

Beta Strand

4..8; 14..22; 45..50; 53..60; 63..71; 172..185; 191..205; 207..209; 211..216; 245..248; 263..265; 269..279; 287..296; 300..309; 315..325; 330..345; 350..372; 386..394; 401..407; 409..412; 418..433

3D Structure

X-ray crystallography (3)

Domain & Motif Annotations

Domain (FT)

170..434; MHD

Protein Families

Adaptor complexes medium subunit family

Sequence Similarities

Belongs to the adaptor complexes medium subunit family.

Clinical Relevance6

Disease Involvement (3)

Disease variantEpilepsyIntellectual disability

Drugs (2)

HISTIDINE [14C]HISTIDINE

Antibody (2)

HPA036849 HPA069870

Interaction Protein (16)

ENSG00000004975ENSG00000006125ENSG00000017797ENSG00000042753ENSG00000085832ENSG00000089818ENSG00000108309ENSG00000114416ENSG00000117597ENSG00000129245ENSG00000146648ENSG00000157191ENSG00000175416ENSG00000198925ENSG00000205726ENSG00000206560

Interaction Count

Interaction Dataset (3)

intact_biogridintact_biogrid_opencellbiogrid_opencell

Supporting Publications2

PMID	Title	Abstract
37027237	Aquaporin 5-in Extracellular Vesicles of Human Vitreous as a Potential Marker for Fungal Endophthalmitis.	No abstract available
37268950	Dental pulp stem cell-derived exosomes revitalize salivary gland epithelial cell function in NOD mice via the GPER-mediated cAMP/PKA/CREB signaling pathway.	Pathway enrichment analysis revealed that DEGs were mainly attributed to estrogen 16 alpha-hydroxylase activity, extracellular exosome function, cAMP signaling, salivary secretion, and estrogen signaling.