Protein detail

NTNG2

Netrin-G2 (Laminet-2)

Entry name NTNG2	UniProt ID Q96CW9	EVMP confidence score 0.38
Supporting publications (n) 3	Transmembrane count	Protein classification Disease related genesHuman disease related genesPredicted intracellular proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

Netrin-G2 (Laminet-2)

Protein Class (3)

Disease related genesHuman disease related genesPredicted intracellular proteins

Protein Function (3)

Disease related genes
Human disease related genes:Congenital malformations:Congenital malformations of the nervous system
Predicted intracellular proteins

Ensembl

ENSG00000196358

Entrez Gene Symbol

NTNG2

Gene Synonym (3)

KIAA1857Lmnt2NTNG1

Gene Description

Netrin G2

Chromosome

Position

132162058-132244526

Supporting publications (n)

EVMP confidence score

0.38

Fluorescence & Localization3

Tissue SpecificbrainCell SpecificBrain excitatory neurons

Function & Pathway7

Protein Function (3)

Disease related genes
Human disease related genes:Congenital malformations:Congenital malformations of the nervous system
Predicted intracellular proteins

Cellular Component (7)

Molecular Function (2)

Biological Process (3)

KEGG (2)

Reactome (2)

Mediation Categories (2)

Adhesion and uptake mediationMetabolism mediation

Relations & Evidence39

Ligand-Receptor Signaling (35)

35 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
ligand	ligand	CellTalkDB	Yes	No	Yes	No	No
ligand	ligand	Ramilowski2015	Yes	No	Yes	No	No
ligand	ligand	LRdb	Yes	No	Yes	No	No
ligand	ligand	OmniPath	Yes	No	Yes	No	No
receptor	receptor	CellCellInteractions	No	Yes	Yes	No	No

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Regulatory Interaction Network (1)

1 record.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
NTNG2	Q96CW9	LRRC4	Q9HBW1	Yes	Yes	No	Fantom5_LRdbCellTalkDBiTALKtalklrRamilowski2015_Baccin2019SIGNORconnectomeDB2020HINTBaccin2019IntActLit-BM-17CellinkerLRdbSTRING_talklrEMBRACERamilowski2015	Lit-BM-17:21946559 LRdb:17973922 IntAct:21946559 Ramilowski2015:17973922 SIGNOR:19467332 HINT:21946559 connectomeDB2020:17973922 Cellinker:25411505 talklr:17973922 CellTalkDB:17973922

Protein Complex Composition (2)

2 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
	NTNG2	Q96CW9	2	PDB	PDB:3zyg
	LRRC4 NTNG2	Q96CW9 Q9HBW1	1:1	PDB	PDB:3zyi

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationDensity Gradient CentrifugationSize Exclusion ChromatographyImmunoaffinity Capture	Mass spectrometryWestern blottingELISA	4	24505114 37686366 40098346 31588238

Sequence, Structure & Domains12

Sequences

Length

530

Mass

59,799

Sequence

MLHLLALFLHCLPLASGDYDICKSWVTTDEGPTWEFYACQPKVMRLKDYVKVKVEPSGITCGDPPERFCSHENPYLCSNECDASNPDLAHPPRLMFDKEEEGLATYWQSITWSRYPSPLEANITLSWNKTVELTDDVVMTFEYGRPTVMVLEKSLDNGRTWQPYQFYAEDCMEAFGMSARRARDMSSSSAHRVLCTEEYSRWAGSKKEKHVRFEVRDRFAIFAGPDLRNMDNLYTRLESAKGLKEFFTLTDLRMRLLRPALGGTYVQRENLYKYFYAISNIEVIGRCKCNLHANLCSMREGSLQCECEHNTTGPDCGKCKKNFRTRSWRAGSYLPLPHGSPNACATAGSFGNCECYGHSNRCSYIDFLNVVTCVSCKHNTRGQHCQHCRLGYYRNGSAELDDENVCIECNCNQIGSVHDRCNETGFCECREGAAGPKCDDCLPTHYWRQGCYPNVCDDDQLLCQNGGTCLQNQRCACPRGYTGVRCEQPRCDPADDDGGLDCDRAPGAAPRPATLLGCLLLLGLAARLGR

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q96CW9-1; Sequence=Displayed; Name=2; IsoId=Q96CW9-2; Sequence=VSP_013147, VSP_013148

Alternative Sequence

352..422; NCECYGHSNRCSYIDFLNVVTCVSCKHNTRGQHCQHCRLGYYRNGSAELDDENVCIECNCNQIGSVHDRCN -> TLQTPPPGRSPSALRGSRRGLANVKEPAGSRPQISEMLLGCTVTLHQGSVGPHIPPKLSLPDPGGPWLGSQ (in isoform 2); 423..530; Missing (in isoform 2)

3D Structural Models

Turn

74..76; 86..88; 113..116; 198..204; 224..227; 336..338

Helix

46..49; 92..95; 171..175; 182..184; 187..189; 216..223; 230..239; 243..246; 271..273

Beta Strand

22..28; 31..36; 51..56; 66..68; 71..73; 79..81; 83..85; 107..109; 121..133; 137..143; 147..156; 162..170; 205..207; 210..213; 248..258; 277..282; 284..287; 296..299; 302..305; 311..313; 324..326

3D Structure

X-ray crystallography (3)

Domain & Motif Annotations

Domain (CC)

The laminin N-terminal domain mediates 1:1 binding to NGL ligand with sub-micromolar affinity. Three NGL-binding loops mediate discrimination for LRRC4C/NGL1 among other NGLs by binding specifically to its LRR repeats. This specificity drives the sorting of a mixed population of molecules into discrete cell surface subdomains.

Domain (FT)

35..286; Laminin N-terminal; 287..346; Laminin EGF-like 1; 353..408; Laminin EGF-like 2; 409..453; Laminin EGF-like 3

Region

69..88; NGL discriminant loop I; 201..203; NGL discriminant loop II; 264..267; NGL discriminant loop III

Clinical Relevance2

Disease Involvement (3)

Autism spectrum disorderDisease variantIntellectual disability

Antibody (2)

HPA024306 HPA071290

Supporting Publications3

PMID	Title	Abstract
31805958	Proteomic analysis of cerebrospinal fluid extracellular vesicles reveals synaptic injury, inflammation, and stress response markers in HIV patients with cognitive impairment.	No abstract available
37926756	Extracellular vesicles from non-neuroendocrine SCLC cells promote adhesion and survival of neuroendocrine SCLC cells.	No abstract available
38207106	Proteomic, Metabolomic, and Fatty Acid Profiling of Small Extracellular Vesicles from Glioblastoma Stem-Like Cells and Their Role in Tumor Heterogeneity.	No abstract available