Protein detail

KCNS1

Delayed-rectifier potassium channel regulatory subunit KCNS1 (Delayed-rectifier K(+) channel alpha subunit 1) (Delayed-rectifier potassium channel subunit Kv9.1) (hKv9.1) (Potassium voltage-gated channel modifier subfamily S member 1)

Entry name KCNS1	UniProt ID Q96KK3	EVMP confidence score 0.50
Supporting publications (n) 1	Transmembrane count 6	Protein classification

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information8

Protein Names

Protein Function (2)

Transporters:Transporter channels and pores
Voltage-gated ion channels:Voltage-Gated Potassium Channels

Transmembrane

218..239; Helical; Name=Segment S1; 271..293; Helical; Name=Segment S2; 305..322; Helical; Name=Segment S3; 338..358; Helical; Voltage-sensor; Name=Segment S4; 374..395; Helical; Name=Segment S5; 436..464; Helical; Name=Segment S6

Transmembrane Count

Ensembl

ENSG00000124134

Entrez Gene Symbol

KCNS1

Supporting publications (n)

EVMP confidence score

0.50

Fluorescence & Localization1

Function & Pathway6

Protein Function (2)

Transporters:Transporter channels and pores
Voltage-gated ion channels:Voltage-Gated Potassium Channels

Cellular Component (4)

Molecular Function (2)

Biological Process (3)

Reactome (3)

Mediation Categories

Fusion and delivery mediation

Relations & Evidence29

Ligand-Receptor Signaling (28)

28 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
potassium	ion_channel	Almen2009	No	Yes	No	No	No
voltage_gated	ion_channel	Almen2009	No	Yes	No	No	No
voltage_gated	ion_channel	Surfaceome	No	Yes	No	No	No
transporter	transporter	OmniPath	No	Yes	No	No	No
ion_channel	ion_channel	OmniPath	No	Yes	No	No	No
transmembrane	transmembrane	UniProt_location	No	No	No	No	No
transmembrane	transmembrane	UniProt_topology	No	No	No	No	No
transmembrane	transmembrane	UniProt_keyword	No	No	No	No	No
transmembrane	transmembrane	Ramilowski_location	No	No	No	No	No
transmembrane	transmembrane	OmniPath	No	No	No	No	No

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Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationUltrafiltration / Tangential Flow FiltrationSize Exclusion Chromatography	Mass spectrometryWestern blottingMORPHELISA	6	39873726 32795414 38731868 40784529 37686366 39835386

Sequence, Structure & Domains9

Sequences

Length

526

Mass

58,372

Sequence

MLMLLVRGTHYENLRSKVVLPTPLGGRSTETFVSEFPGPDTGIRWRRSDEALRVNVGGVRRQLSARALARFPGTRLGRLQAAASEEQARRLCDDYDEAAREFYFDRHPGFFLSLLHFYRTGHLHVLDELCVFAFGQEADYWGLGENALAACCRARYLERRLTQPHAWDEDSDTPSSVDPCPDEISDVQRELARYGAARCGRLRRRLWLTMENPGYSLPSKLFSCVSISVVLASIAAMCIHSLPEYQAREAAAAVAAVAAGRSPEGVRDDPVLRRLEYFCIAWFSFEVSSRLLLAPSTRNFFCHPLNLIDIVSVLPFYLTLLAGVALGDQGGKEFGHLGKVVQVFRLMRIFRVLKLARHSTGLRSLGATLKHSYREVGILLLYLAVGVSVFSGVAYTAEKEEDVGFNTIPACWWWGTVSMTTVGYGDVVPVTVAGKLAASGCILGGILVVALPITIIFNKFSHFYRRQKALEAAVRNSNHQEFEDLLSSIDGVSEASLETSRETSQEGQSADLESQAPSEPPHPQMY

Domain & Motif Annotations

Compositional Bias

505..517; Polar residues

Motif

421..426; Selectivity filter

Domain (CC)

The transmembrane segment S4 functions as a voltage-sensor and is characterized by a series of positively charged amino acids at every third position. Channel opening and closing is effected by a conformation change that affects the position and orientation of the voltage-sensor paddle formed by S3 and S4 within the membrane. A transmembrane electric field that is positive inside would push the positively charged S4 segment outwards, thereby opening the pore, while a field that is negative inside would pull the S4 segment inwards and close the pore. Changes in the position and orientation of S4 are then transmitted to the activation gate formed by the inner helix bundle via the S4-S5 linker region.

Region

493..526; Disordered

Protein Families (3)

Potassium channel family
S (TC 1.A.1.2) subfamily
Kv9.1/KCNS1 sub-subfamily

Sequence Similarities

Belongs to the potassium channel family. S (TC 1.A.1.2) subfamily. Kv9.1/KCNS1 sub-subfamily.

Clinical Relevance1

Drugs (6)

NERISPIRDINE DALFAMPRIDINE AMIFAMPRIDINE GUANIDINE HYDROCHLORIDE TEDISAMIL AMIFAMPRIDINE PHOSPHATE

Supporting Publications1

PMID	Title	Abstract
19837982	Proteomics analysis of A33 immunoaffinity-purified exosomes released from the human colon tumor cell line LIM1215 reveals a tissue-specific protein signature.	A conspicuous finding of this comparative analysis was the presence of host cell-specific (LIM1215 exosome) proteins such as A33, cadherin-17, carcinoembryonic antigen, epithelial cell surface antigen (EpCAM), proliferating cell nuclear antigen, epidermal growth factor receptor, mucin 13, misshapen-like kinase 1, keratin 18, mitogen-activated protein kinase 4, claudins (1, 3, and 7), centrosomal protein 55 kDa, and ephrin-B1 and -B2. Here, we describe an immunoaffinity capture method using the colon epithelial cell-specific A33 antibody to purify colorectal cancer cell (LIM1215)-derived exosomes.