Protein detail

PCMD1

Protein-L-isoaspartate O-methyltransferase domain-containing protein 1

Entry name PCMD1	UniProt ID Q96MG8	EVMP confidence score 0.50
Supporting publications (n) 1	Transmembrane count	Protein classification Predicted intracellular proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

Protein-L-isoaspartate O-methyltransferase domain-containing protein 1

Protein Class

Predicted intracellular proteins

Protein Function

Predicted intracellular proteins

Ensembl

ENSG00000168300

Entrez Gene Symbol

PCMTD1

Gene Synonym

FLJ10883

Gene Description

Protein-L-isoaspartate (D-aspartate) O-methyltransferase domain containing 1

Chromosome

Position

51817575-51899186

Supporting publications (n)

EVMP confidence score

0.50

Fluorescence & Localization2

Tissue Specificadrenal glandCell SpecificAdrenal cortex cells

Function & Pathway6

Protein Function

Predicted intracellular proteins

Cellular Component (3)

Molecular Function (3)

Biological Process (3)

Canonical Pathways (3)

M92 Pid angiopoietin receptor pathway
M122 Pid il2 1pathway
M28 Pid il4 2pathway

Mediation Categories

Other mediation

Relations & Evidence6

Ligand-Receptor Signaling (4)

4 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No

Protein Complex Composition (1)

1 record.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
	CUL5 ELOB ELOC PCMTD1 RNF7	Q15369 Q15370 Q93034 Q96MG8 Q9UBF6	1:1:1:1:1	PDB	PDB:9oma

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion ChromatographyImmunoaffinity Capture	Western blotting	2	38731868 40098346

Sequence, Structure & Domains11

Sequences

Length

357

Mass

40,675

Sequence

MGGAVSAGEDNDDLIDNLKEAQYIRTERVEQAFRAIDRGDYYLEGYRDNAYKDLAWKHGNIHLSAPCIYSEVMEALKLQPGLSFLNLGSGTGYLSTMVGLILGPFGINHGIELHSDVVEYAKEKLESFIKNSDSFDKFEFCEPAFVVGNCLQIASDSHQYDRIYCGAGVQKDHENYMKILLKVGGILVMPIEDQLTQIMRTGQNTWESKNILAVSFAPLVQPSKNDNGKPDSVGLPPCAVRNLQDLARIYIRRTLRNFINDEMQAKGIPQRAPPKRKRKRVKQRINTYVFVGNQLIPQPLDSEEDEKMEEDNKEEEEKDHNEAMKPEEPPQNLLREKIMKLPLPESLKAYLTYFRDK

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q96MG8-1; Sequence=Displayed; Name=2; IsoId=Q96MG8-2; Sequence=VSP_061574

Alternative Sequence

1..137; MGGAVSAGEDNDDLIDNLKEAQYIRTERVEQAFRAIDRGDYYLEGYRDNAYKDLAWKHGNIHLSAPCIYSEVMEALKLQPGLSFLNLGSGTGYLSTMVGLILGPFGINHGIELHSDVVEYAKEKLESFIKNSDSFDK -> MPGPRRLRSPGGSGCSSRGSGPRLSTRRWRPCCYCGGSGVWLLRTPPSPSAWPARRCPRHR (in isoform 2)

3D Structural Models

3D Structure

Electron microscopy (2)

Domain & Motif Annotations

Compositional Bias

301..317; Acidic residues; 318..333; Basic and acidic residues

Domain (CC)

At its N-terminus, contains L-isoaspartate and S-adenosylmethionine (AdoMet) binding motifs. Also contains an extended SOCS box motif, where the Cul-box is separated from the BC-box by ~90 residues, within its C-terminus.

Region

85..94; AdoMet binding motif; 160..164; AdoMet binding motif; 181..191; AdoMet binding motif; 240..250; BC-box; 299..333; Disordered; 341..344; CUL-box

Protein Families (2)

Methyltransferase superfamily
L-isoaspartyl/D-aspartyl protein methyltransferase family

Sequence Similarities

Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.

Clinical Relevance1

Antibody (2)

HPA025696 HPA061444

Supporting Publications1

PMID	Title	Abstract
24069378	Proteome profiling of neuroblastoma-derived exosomes reveal the expression of proteins potentially involved in tumor progression.	We found that the large majority of the proteins identified in NB derived exosomes are present in Exocarta database including tetraspanins, fibronectin, heat shock proteins, MVB proteins, cytoskeleton-related proteins, prominin-1 (CD133), basigin (CD147) and B7-H3 (CD276).