Protein detail

ROBO3

Roundabout homolog 3 (Roundabout-like protein 3)

Entry name ROBO3	UniProt ID Q96MS0	EVMP confidence score 0.50
Supporting publications (n) 3	Transmembrane count 1	Protein classification

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information8

Protein Names

Roundabout homolog 3 (Roundabout-like protein 3)

Protein Function (3)

Disease related genes
Human disease related genes:Nervous system diseases:Other nervous and sensory system diseases
Predicted intracellular proteins

Transmembrane

892..912; Helical

Transmembrane Count

Ensembl

ENSG00000154134

Entrez Gene Symbol

ROBO3

Supporting publications (n)

EVMP confidence score

0.50

Fluorescence & Localization4

Tissue Specificadrenal glandCell SpecificCardiomyocytesSingle-Nuclei Brain Specificoligodendrocyte precursor cell

Function & Pathway6

Protein Function (3)

Disease related genes
Human disease related genes:Nervous system diseases:Other nervous and sensory system diseases
Predicted intracellular proteins

Cellular Component (3)

Molecular Function (2)

KEGG (2)

Reactome (5)

Mediation Categories

Adhesion and uptake mediation

Relations & Evidence49

Enzyme-Mediated Modification (2)

2 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
ROBO3	PRKCA	P17252	S	1,330	phosphorylation	PhosphoSitePhosphoSite_ProtMapperProtMapper
ROBO3	SRC	P12931	Y	1,019	phosphorylation	Reactome_ProtMapperProtMapper

Ligand-Receptor Signaling (41)

41 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
extracellular	extracellular	HPMR	No	No	No	Yes	No
extracellular	extracellular	OmniPath	No	No	No	Yes	No
intracellular	intracellular	ComPPI	No	No	No	Yes	No
intracellular	intracellular	OmniPath	No	No	No	Yes	No
cell_adhesion	cell_adhesion	Cellinker	Yes	Yes	No	Yes	No
adhesion	adhesion	OmniPath	Yes	Yes	No	Yes	No
cell_adhesion	cell_adhesion	OmniPath	Yes	Yes	No	Yes	No
transmembrane	transmembrane	UniProt_location	No	No	No	Yes	No
transmembrane	transmembrane	UniProt_topology	No	No	No	Yes	No
transmembrane	transmembrane	UniProt_keyword	No	No	No	Yes	No

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Regulatory Interaction Network (3)

3 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
ROBO3	Q96MS0	COF1	P23528	Yes	Yes	No	SIGNOR	SIGNOR:16226035
SLIT2	O94813	ROBO3	Q96MS0	Yes	Yes	No	SIGNOR	SIGNOR:16226035
KPCA	P17252	ROBO3	Q96MS0	Yes	No	No	iPTMnetPhosphoSitePhosphoSite_ProtMapperProtMapper	PhosphoSite:25882844

Protein Complex Composition (2)

2 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
	NELL1 ROBO3	Q92832 Q96MS0	3:3	PDB	PDB:6pol
	NELL2 ROBO3	Q96MS0 Q99435	1:1	PDB	PDB:6pog

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion Chromatography	Mass spectrometryWestern blottingELISA	5	38225453 38731868 26775013 37686366 37922300

Sequence, Structure & Domains14

Sequences

Length

1,386

Mass

148,209

Sequence

MLRYLLKTLLQMNLFADSLAGDISNSSELLLGFNSSLAALNHTLLPPGDPSLNGSRVGPEDAMPRIVEQPPDLLVSRGEPATLPCRAEGRPRPNIEWYKNGARVATVREDPRAHRLLLPSGALFFPRIVHGRRARPDEGVYTCVARNYLGAAASRNASLEVAVLRDDFRQSPGNVVVAVGEPAVLECVPPRGHPEPSVSWRKDGARLKEEEGRITIRGGKLMMSHTLKSDAGMYVCVASNMAGERESAAAEVMVLERPSFLRRPVNQVVLADAPVTFLCEVKGDPPPRLRWRKEDGELPTGRYEIRSDHSLWIGHVSAEDEGTYTCVAENSVGRAEASGSLSVHVPPQLVTQPQDQMAAPGESVAFQCETKGNPPPAIFWQKEGSQVLLFPSQSLQPTGRFSVSPRGQLNITAVQRGDAGYYVCQAVSVAGSILAKALLEIKGASLDGLPPVILQGPANQTLVLGSSVWLPCRVTGNPQPSVRWKKDGQWLQGDDLQFKTMANGTLYIANVQEMDMGFYSCVAKSSTGEATWSGWLKMREDWGVSPDPPTEPSSPPGAPSQPVVTEITKNSITLTWKPNPQTGAAVTSYVIEAFSPAAGNTWRTVADGVQLETHTVSGLQPNTIYLFLVRAVGAWGLSEPSPVSEPVRTQDSSPSRPVEDPWRGQQGLAEVAVRLQEPIVLGPRTLQVSWTVDGPVQLVQGFRVSWRVAGPEGGSWTMLDLQSPSQQSTVLRGLPPGTQIQIKVQAQGQEGLGAESLSVTRSIPEEAPSGPPQGVAVALGGDGNSSITVSWEPPLPSQQNGVITEYQIWCLGNESRFHLNRSAAGWARSAMLRGLVPGLLYRTLVAAATSAGVGVPSAPVLVQLPSPPDLEPGLEVGAGLAVRLARVLREPAFLAGSGAACGALLLGLCAALYWRRKQRKELSHYTASFAYTPAVSFPHSEGLSGASSRPPMGLGPAPYSWLADSWPHPSRSPSAQEPRGSCCPSNPDPDDRYYNEAGISLYLAQTARGTAAPGEGPVYSTIDPAGEELQTFHGGFPQHPSGDLGPWSQYAPPEWSQGDSGAKGGKVKLLGKPVQMPSLNWPEALPPPPPSCELSCLEGPEEELEGSSEPEEWCPPMPERSHLTEPSSSGGCLVTPSRRETPSPTPSYGQQSTATLTPSPPDPPQPPTDMPHLHQMPRRVPLGPSSPLSVSQPMLGIREARPAGLGAGPAASPHLSPSPAPSTASSAPGRTWQGNGEMTPPLQGPRARFRKKPKALPYRRENSPGDLPPPPLPPPEEEASWALELRAAGSMSSLERERSGERKAVQAVPLAAQRVLHPDEEAWLPYSRPSFLSRGQGTSTCSTAGSNSSRGSSSSRGSRGPGRSRSRSQSRSQSQRPGQKRREEPR

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; Synonyms=Robo3.1; IsoId=Q96MS0-1; Sequence=Displayed; Name=2; IsoId=Q96MS0-2; Sequence=VSP_010650, VSP_010651

Alternative Sequence

1025..1034; AGEELQTFHG -> LTTPLLILTT (in isoform 2); 1035..1386; Missing (in isoform 2)

3D Structural Models

Turn

596..598

Helix

696..698; 796..799; 815..817

Beta Strand

563..567; 572..575; 588..594; 603..609; 611..616; 624..633; 672..675; 686..694; 701..708; 717..721; 728..731; 738..747; 759..762; 773..779; 782..785; 787..792; 805..811; 819..824; 829..832; 840..849; 852..856; 860..863

3D Structure

X-ray crystallography (3)

Domain & Motif Annotations

Compositional Bias

546..559; Pro residues; 1067..1083; Low complexity; 1099..1112; Acidic residues; 1158..1169; Pro residues; 1178..1191; Low complexity; 1202..1229; Low complexity; 1294..1304; Basic and acidic residues; 1333..1344; Polar residues; 1345..1361; Low complexity

Domain (FT)

64..160; Ig-like C2-type 1; 166..253; Ig-like C2-type 2; 258..342; Ig-like C2-type 3; 347..440; Ig-like C2-type 4; 450..531; Ig-like C2-type 5; 558..652; Fibronectin type-III 1; 671..766; Fibronectin type-III 2; 771..869; Fibronectin type-III 3

Region

541..563; Disordered; 639..662; Disordered; 965..989; Disordered; 1028..1310; Disordered; 1327..1386; Disordered

Protein Families (2)

Immunoglobulin superfamily
ROBO family

Sequence Similarities

Belongs to the immunoglobulin superfamily. ROBO family.

Supporting Publications3

PMID	Title	Abstract
24505114	Proteomics analysis of cancer exosomes using a novel modified aptamer-based array (SOMAscan™) platform.	These included proteins of known association with cancer exosomes such as MFG-E8, integrins, and MET, and also those less widely reported as exosomally associated, such as ROR1 and ITIH4.
31588238	Dual-platform affinity proteomics identifies links between the recurrence of ovarian carcinoma and proteins released into the tumor microenvironment.	No abstract available
40098346	Toward Identification of Markers for Brain-Derived Extracellular Vesicles in Cerebrospinal Fluid: A Large-Scale, Unbiased Analysis Using Proximity Extension Assays.	No abstract available