Protein detail

ARAP1

Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 (Centaurin-delta-2) (Cnt-d2)

Entry name
ARAP1
UniProt ID
EVMP confidence score
0.72
Supporting publications (n)
11
Transmembrane count
Protein classification
Predicted intracellular proteins
EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40
Basic Information11
Protein Names
Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 (Centaurin-delta-2) (Cnt-d2)
Protein Class
Predicted intracellular proteins
Protein Function
Predicted intracellular proteins
Entrez Gene Symbol
Gene Synonym
CENTD2
Gene Description
ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1
Chromosome
11
Position
72685069-72793599
Supporting publications (n)
11
EVMP confidence score
0.72
Fluorescence & Localization4
ARAP1 fluorescence
Cell SpecificChoroid plexus epithelial cellsBlood Cell SpecificbasophilBlood Lineage Specificgranulocytes
Function & Pathway7
Relations & Evidence14

Enzyme-Mediated Modification (2)

2 records.

Substrate Gene SymbolEnzyme Gene SymbolEnzyme UniProt IDResidue TypeResidue OffsetModificationDatabaseReferences
ARAP1PTK6Q13882Y231phosphorylationphosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperRLIMS-P_ProtMapperPhosphoSitePhosphoSite_ProtMapperProtMapper:20554524SIGNOR:20554524
ARAP1HIPK4Q8NE63S648phosphorylationPhosphoNetworks

Ligand-Receptor Signaling (6)

6 records.

CategoryParentDatabaseTransmitterReceiverSecretedPlasma Membrane (Transmembrane)Plasma Membrane (Peripheral)
intracellularintracellularComPPINoNoNoNoNo
intracellularintracellularGO_IntercellNoNoNoNoNo
intracellularintracellularUniProt_locationNoNoNoNoNo
intracellularintracellularOmniPathNoNoNoNoNo
plasma_membraneplasma_membraneUniProt_locationNoNoNoNoNo
plasma_membraneplasma_membraneOmniPathNoNoNoNoNo

Regulatory Interaction Network (3)

3 records.

Source Protein SymbolSource UniProt IDTarget Protein SymbolTarget UniProt IDIs DirectedIs StimulationIs InhibitionDatabaseReferences
ARAP1Q96P48CDC42P60953YesNoYesSIGNORSIGNOR:32203420
PTK6Q13882ARAP1Q96P48YesYesNophosphoELM_MIMPPhosphoSite_MIMPMIMPHPRD_MIMPiPTMnetSIGNORProtMapperRLIMS-P_ProtMapperSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapperPhosphoSite:20554524ProtMapper:20554524SIGNOR:20554524
ARAP1Q96P48RAC1P63000YesNoYesWangSIGNORSIGNOR:32203420

Protein Complex Composition (2)

2 records.

Component NameComponent Gene SymbolsComponent UniProt IDStoichiometryDatabaseDatabase IDsReferences
ARAP1DOK1NCK2WIPF3A6NGB9O43639Q96P48Q997040:0:0:0hu.MAP
ARAP1GRB2INPP5DSH3KBP1P62993Q92835Q96B97Q96P481:1:1:1CompleatCFinderCompleat:HC8471

Isolation & Detection Technology (1)

1 record.

EV Isolation MethodDetection MethodNumber of ReferencesReferences
Differential UltracentrifugationSize Exclusion ChromatographyImmunoaffinity CaptureMass spectrometry1338113368373224753359250032854315380145954130796840840701322309703457624639001700374386383920704741068253
Sequence, Structure & Domains11

Sequences

Length
1,450
Mass
162,192
Sequence
MAEAGDAALSVAEWLRALHLEQYTGLFEQHGLVWATECQGLSDTRLMDMGMLLPGHRRRILAGLLRAHTSPAPAPRPTPRPVPMKRHIFRSPPVPATPPEPLPTTTEDEGLPAAPPIPPRRSCLPPTCFTTPSTAAPDPVLPPLPAKRHLAELSVPPVPPRTGPPRLLVSLPTKEEESLLPSLSSPPQPQSEEPLSTLPQGPPQPPSPPPCPPEIPPKPVRLFPEFDDSDYDEVPEEGPGAPARVMTKKEEPPPSRVPRAVRVASLLSEGEELSGDDQGDEEEDDHAYEGVPNGGWHTSSLSLSLPSTIAAPHPMDGPPGGSTPVTPVIKAGWLDKNPPQGSYIYQKRWVRLDTDHLRYFDSNKDAYSKRFISVACISHVAAIGDQKFEVITNNRTFAFRAESDVERKEWMQALQQAMAEQRARARLSSAYLLGVPGSEQPDRAGSLELRGFKNKLYVAVVGDKVQLYKNLEEYHLGIGITFIDMSVGNVKEVDRRSFDLTTPYRIFSFSADSELEKEQWLEAMQGAIAEALSTSEVAERIWAAAPNRFCADCGAPQPDWASINLCVVICKRCAGEHRGLGAGVSKVRSLKMDRKVWTETLIELFLQLGNGAGNRFWAANVPPSEALQPSSSPSTRRCHLEAKYREGKYRRYHPLFGNQEELDKALCAAVTTTDLAETQALLGCGAGINCFSGDPEAPTPLALAEQAGQTLQMEFLRNNRTTEVPRLDSMKPLEKHYSVVLPTVSHSGFLYKTASAGKLLQDRRAREEFSRRWCVLGDGVLSYFENERAVTPNGEIRASEIVCLAVPPPDTHGFEHTFEVYTEGERLYLFGLESAEQAHEWVKCIAKAFVPPLAEDLLARDFERLGRLPYKAGLSLQRAQEGWFSLSGSELRAVFPEGPCEEPLQLRKLQELSIQGDSENQVLVLVERRRTLYIQGERRLDFMGWLGAIQKAAASMGDTLSEQQLGDSDIPVIVYRCVDYITQCGLTSEGIYRKCGQTSKTQRLLESLRQDARSVHLKEGEQHVDDVSSALKRFLRDLPDGLFTRAQRLTWLEASEIEDEEEKVSRYRELLVRLPPVNRATVKALISHLYCVQCFSDTNQMNVHNLAIVFGPTLFQTDGQDYKAGRVVEDLINHYVVVFSVDEEELRKQREEITAIVKMRVAGTASGTQHAGDFICTVYLEEKKAETEQHIKVPASMTAEELTLEILDRRNVGIREKDYWTCFEVNEREEAERPLHFAEKVLPILHGLGTDSHLVVKKHQAMEAMLLYLASRVGDTKHGMMKFREDRSLLGLGLPSGGFHDRYFILNSSCLRLYKEVRSQRPWSGAPETSHRPEKEWPIKSLKVYLGVKKKLRPPTCWGFTVVHETEKHEKQQWYLCCDTQMELREWFATFLFVQHDGLVWPSEPSRVSRAVPEVRLGSVSLIPLRGSENEMRRSVAAFTADPLSLLRNV
Alternative Products
Event=Alternative splicing; Named isoforms=7; Name=6; Synonyms=ARAP1b; IsoId=Q96P48-6; Sequence=Displayed; Name=1; IsoId=Q96P48-1; Sequence=VSP_036607, VSP_036608; Name=2; IsoId=Q96P48-2; Sequence=VSP_036607, VSP_036608, VSP_000311; Name=3; IsoId=Q96P48-3; Sequence=VSP_000311; Name=4; IsoId=Q96P48-4; Sequence=VSP_015000; Name=5; IsoId=Q96P48-5; Sequence=VSP_014998, VSP_015001, VSP_000311; Name=7; IsoId=Q96P48-7; Sequence=VSP_015000, VSP_043530, VSP_000311
Alternative Sequence
1..760; Missing (in isoform 5); 1..245; Missing (in isoform 4 and isoform 7); 1..240; Missing (in isoform 1 and isoform 2); 241..249; APARVMTKK -> MTLSGSRGQ (in isoform 1 and isoform 2); 604..664; Missing (in isoform 7); 761..767; QDRRARE -> MDASGKG (in isoform 5); 1320..1330; Missing (in isoform 2, isoform 3, isoform 5 and isoform 7)

3D Structural Models

3D Structure
X-ray crystallography (1)

Domain & Motif Annotations

Compositional Bias
92..102; Pro residues; 190..199; Low complexity; 200..219; Pro residues; 225..236; Acidic residues; 269..286; Acidic residues
Zinc Finger
550..576; C4-type
Domain (CC)
The first PH domain, PH 1, interacts with PtdIns(3,4,5)P3 which stimulates ARAP1 GTPase-activating activity and is also required for ARAP1-mediated regulation of endocytic trafficking of EGFR (PubMed:18939958, PubMed:19666464). It does not mediate PtdIns(3,4,5)P3-dependent recruitment of ARAP1 to membranes although this may be mediated by other PH domains (PubMed:19666464).
Domain (FT)
6..70; SAM; 327..419; PH 1; 440..529; PH 2; 535..660; Arf-GAP; 743..850; PH 3; 954..1139; Rho-GAP; 1172..1261; Ras-associating; 1274..1396; PH 4
Region
81..90; Required for interaction with SH3KBP1; 89..144; Disordered; 173..302; Disordered
Clinical Relevance5
Interaction Protein (2)
ENSG00000077549ENSG00000196419
Interaction Count
2
Interaction Dataset (2)
biogrid_opencellintact_biogrid
Supporting Publications11
PMIDTitleAbstract
32854315Proteomic Profiling of Extracellular Vesicles Derived from Cerebrospinal Fluid of Alzheimer's Disease Patients: A Pilot Study.Recent studies have highlighted the importance of Aβ and tau-containing extracellular vesicles (EVs) in AD.
36064647Systemic proteomics and miRNA profile analysis of exosomes derived from human pluripotent stem cells.No abstract available
37322475Comprehensive profiling of extracellular vesicles in uveitis and scleritis enables biomarker discovery and mechanism exploration.No abstract available
38113368In-Depth Proteome Profiling of Small Extracellular Vesicles Isolated from Cancer Cell Lines and Patient Serum.No abstract available
38207106Proteomic, Metabolomic, and Fatty Acid Profiling of Small Extracellular Vesicles from Glioblastoma Stem-Like Cells and Their Role in Tumor Heterogeneity.No abstract available
39207047The trajectory of vesicular proteomic signatures from HBV-HCC by chitosan-magnetic bead-based separation and DIA-proteomic analysis.No abstract available
40189497Small extracellular vesicle-based one-step high-throughput microfluidic platform for epithelial ovarian cancer diagnosis.No abstract available
40465195Extracellular vesicle proteomics uncovers energy metabolism, complement system, and endoplasmic reticulum stress response dysregulation postexercise in males with myalgic encephalomyelitis/chronic fatigue syndrome.No abstract available
40840701Multiomics analysis to evaluate the enrichment of extracellular vesicles from human plasma.No abstract available
40940449Extracellular vesicle-associated transcriptomic and proteomic biomarkers show in vitro potential for vandetanib treatment monitoring in anaplastic thyroid cancer.No abstract available
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