Protein detail
NEB2
Neurabin-2 (Neurabin-II) (Protein phosphatase 1 regulatory subunit 9B) (Spinophilin)
Protein symbol NEB2 | UniProt ID | EVMP score 0.38 |
Frequency | Transmembrane count | Protein classification Predicted intracellular proteins |
Basic Information
Protein Names
Neurabin-2 (Neurabin-II) (Protein phosphatase 1 regulatory subunit 9B) (Spinophilin)
Protein Class
Predicted intracellular proteins
Protein Function
Predicted intracellular proteins
Ensembl
Entrez Gene Symbol
Gene Synonym
PPP1R6PPP1R9SPINOSpn
Gene Description
Protein phosphatase 1 regulatory subunit 9B
Chromosome
17
Position
50133737-50150677
EVMP Score
0.38
Fluorescence & Localization
Tissue SpecificbrainCell SpecificAstrocytesSingle-Nuclei Brain SpecificastrocyteBlood Cell SpecificbasophilBlood Lineage SpecificB-cells
Function & Pathway
Protein Function
Predicted intracellular proteins
Cellular Component
- GO:0000164 protein phosphatase type 1 complex
- GO:0005654 nucleoplasm
- GO:0005737 cytoplasm
- GO:0005886 plasma membrane
- GO:0005912 adherens junction
- GO:0014069 postsynaptic density
- GO:0015629 actin cytoskeleton
- GO:0030027 lamellipodium
- GO:0030175 filopodium
- GO:0030425 dendrite
- GO:0030426 growth cone
- GO:0030864 cortical actin cytoskeleton
- GO:0032587 ruffle membrane
- GO:0043025 neuronal cell body
- GO:0044326 dendritic spine neck
- GO:0044327 dendritic spine head
- GO:0097444 spine apparatus
- GO:0098890 extrinsic component of postsynaptic membrane
- GO:0098978 glutamatergic synapse
- GO:1990780 cytoplasmic side of dendritic spine plasma membrane
Molecular Function
Biological Process
Mediation Categories
Fusion and delivery mediationReceptor-signaling mediation
Relations & Evidence
Enzyme-Mediated Modification
9 records.
| Substrate Gene Symbol | Enzyme Gene Symbol | Enzyme UniProt ID | Residue Type | Residue Offset | Modification | Database | References |
|---|---|---|---|---|---|---|---|
| PPP1R9B | MAPK1 | P28482 | S | 15 | phosphorylation | SIGNORProtMapperHPRDKEASIGNOR_ProtMapperREACH_ProtMapper | ProtMapper:15728359SIGNOR:15728359ProtMapper:15980451HPRD:15728359KEA:15728359ProtMapper:21704645 |
| PPP1R9B | MAPK1 | P28482 | S | 17 | phosphorylation | REACH_ProtMapperProtMapper | ProtMapper:15980451 |
| PPP1R9B | MAPK1 | P28482 | S | 205 | phosphorylation | KEA | KEA:15728359 |
| PPP1R9B | PRKACA | P17612 | S | 94 | phosphorylation | SIGNORProtMapperHPRDKEASIGNOR_ProtMapper | ProtMapper:12417592HPRD:20068231KEA:12417592HPRD:12417592KEA:16300646SIGNOR:12417592 |
| PPP1R9B | PRKACA | P17612 | S | 100 | phosphorylation | HPRDKEA | KEA:12417592HPRD:12417592HPRD:20068231HPRD:18669648 |
| PPP1R9B | CDK5 | Q00535 | S | 15 | phosphorylation | REACH_ProtMapperProtMapper | ProtMapper:15980451 |
| PPP1R9B | CDK5 | Q00535 | S | 17 | phosphorylation | REACH_ProtMapperKEASparser_ProtMapperProtMapper | ProtMapper:28634551ProtMapper:15980451KEA:15728359 |
| PPP1R9B | PRKCB | P05771 | S | 94 | phosphorylation | KEA | KEA:17570479 |
| PPP1R9B | RPS6KA3 | P51812 | S | 100 | phosphorylation | KEA | KEA:17570479 |
Ligand-Receptor Signaling
10 records.
| Category | Parent | Database | Transmitter | Receiver | Secreted | Plasma Membrane (Transmembrane) | Plasma Membrane (Peripheral) |
|---|---|---|---|---|---|---|---|
| intracellular | intracellular | LOCATE | No | No | No | No | No |
| intracellular | intracellular | ComPPI | No | No | No | No | No |
| intracellular | intracellular | GO_Intercell | No | No | No | No | No |
| intracellular | intracellular | UniProt_location | No | No | No | No | No |
| intracellular | intracellular | OmniPath | No | No | No | No | No |
| ion_channel | ion_channel | DGIdb | No | Yes | No | No | No |
| transporter | transporter | OmniPath | No | Yes | No | No | No |
| ion_channel | ion_channel | OmniPath | No | Yes | No | No | No |
| plasma_membrane | plasma_membrane | UniProt_location | No | No | No | No | No |
| plasma_membrane | plasma_membrane | OmniPath | No | No | No | No | No |
Regulatory Interaction Network
6 records.
| Source Protein Symbol | Source UniProt ID | Target Protein Symbol | Target UniProt ID | Is Directed | Is Stimulation | Is Inhibition | Database | References |
|---|---|---|---|---|---|---|---|---|
| NEB2 | Q96SB3 | ARHG2 | Q92974 | Yes | Yes | No | SIGNOR | SIGNOR:15996550 |
| NEB2 | Q96SB3 | SPT13 | Q96N96 | Yes | Yes | No | SIGNOR | SIGNOR:19151759 |
| CDK5 | Q00535 | NEB2 | Q96SB3 | Yes | No | Yes | Sparser_ProtMapperSIGNORProtMapperKEAphosphoELM_KEAREACH_ProtMapper | ProtMapper:28634551ProtMapper:15980451KEA:15728359SIGNOR:29786422ProtMapper:29786422 |
| KAPCA | P17612 | NEB2 | Q96SB3 | Yes | Yes | Yes | WangiPTMnetPhosphoPointSIGNORProtMapperHPRDCui2007PhosphoSite_KEAKEAphosphoELM_KEAHPRD_KEASIGNOR_ProtMapperHPRD-phos | ProtMapper:12417592ProtMapper:20068231HPRD-phos:20068231HPRD-phos:18669648KEA:12417592HPRD:12417592ProtMapper:18669648KEA:16300646SIGNOR:12417592HPRD-phos:12417592 |
| MK01 | P28482 | NEB2 | Q96SB3 | Yes | Yes | No | WangiPTMnetPhosphoPointSIGNORProtMapperHPRDPhosphoSite_KEAKEAphosphoELM_KEAHPRD_KEASIGNOR_ProtMapperREACH_ProtMapperHPRD-phos | ProtMapper:15728359SIGNOR:15728359ProtMapper:15980451HPRD:15728359KEA:15728359ProtMapper:21704645HPRD-phos:15728359 |
| NEB2 | Q96SB3 | TIAM1 | Q13009 | Yes | Yes | No | HPRDSIGNOR | HPRD:12531897SIGNOR:12531897 |
Protein Complex Composition
0 records.
Isolation & Detection Technology
1 record.
| EV Isolation Method | Detection Method | Number of References | References |
|---|---|---|---|
| Size Exclusion Chromatography | Mass spectrometryWestern blotting | 1 | 34265469 |
Sequence, Structure & Domains
Sequences
Length
817
Mass
89,334
Sequence
MMKTEPRGPGGPLRSASPHRSAYEAGIQALKPPDAPGPDEAPKGAHHKKYGSNVHRIKSMFLQMGTTAGPSGEAGGGAGLAEAPRASERGVRLSLPRASSLNENVDHSALLKLGTSVSERVSRFDSKPAPSAQPAPPPHPPSRLQETRKLFERSAPAAAGGDKEAAARRLLRQERAGLQDRKLDVVVRFNGSTEALDKLDADAVSPTVSQLSAVFEKADSRTGLHRGPGLPRAAGVPQVNSKLVSKRSRVFQPPPPPPPAPSGDAPAEKERCPAGQQPPQHRVAPARPPPKPREVRKIKPVEVEESGESEAESAPGEVIQAEVTVHAALENGSTVATAASPAPEEPKAQAAPEKEAAAVAPPERGVGNGRAPDVAPEEVDESKKEDFSEADLVDVSAYSGLGEDSAGSALEEDDEDDEEDGEPPYEPESGCVEIPGLSEEEDPAPSRKIHFSTAPIQVFSTYSNEDYDRRNEDVDPMAASAEYELEKRVERLELFPVELEKDSEGLGISIIGMGAGADMGLEKLGIFVKTVTEGGAAHRDGRIQVNDLLVEVDGTSLVGVTQSFAASVLRNTKGRVRFMIGRERPGEQSEVAQLIQQTLEQERWQREMMEQRYAQYGEDDEETGEYATDEDEELSPTFPGGEMAIEVFELAENEDALSPVDMEPEKLVHKFKELQIKHAVTEAEIQQLKRKLQSLEQEKGRWRVEKAQLEQSVEENKERMEKLEGYWGEAQSLCQAVDEHLRETQAQYQALERKYSKAKRLIKDYQQKEIEFLKKETAQRRVLEESELARKEEMDKLLDKISELEGNLQTLRNSNST
3D Structural Models
Domain & Motif Annotations
Compositional Bias
44..58; Basic residues; 131..141; Pro residues; 252..261; Pro residues; 291..302; Basic and acidic residues; 333..342; Low complexity; 344..356; Basic and acidic residues; 410..425; Acidic residues
Motif
447..451; PP1-binding motif
Coiled Coil
671..788
Domain (CC)
The PP1 binding region is natively unstructured, upon PP1 binding, it acquires structure, blocks a substrate-binding site, and restricts PP1 phosphatase specificity to a subset of substrates.
Domain (FT)
496..584; PDZ
Region
1..165; Disordered; 1..154; Actin-binding; 100..371; Interaction with D(2) dopamine receptor; 164..283; Actin-binding; 169..255; Interaction with ADRA2A, ADRA2B and ADRA2C; 216..447; Disordered; 417..494; Interaction with protein phosphatase 1; 480..525; Interaction with RGS2; 595..816; Interaction with TGN38
Clinical Relevance
Antibody
Interaction Protein
ENSG00000012048ENSG00000130402ENSG00000172531ENSG00000173548ENSG00000186298ENSG00000213639
Interaction Count
6
Interaction Dataset
intact_biogridbiogrid_opencellbiogrid_bioplex