Protein detail

LITAF

Lipopolysaccharide-induced tumor necrosis factor-alpha factor (LPS-induced TNF-alpha factor) (Small integral membrane protein of lysosome/late endosome) (p53-induced gene 7 protein)

Entry name LITAF	UniProt ID Q99732	EVMP confidence score 0.50
Supporting publications (n) 6	Transmembrane count	Protein classification Disease related genesHuman disease related genesPredicted intracellular proteinsPredicted membrane proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

Lipopolysaccharide-induced tumor necrosis factor-alpha factor (LPS-induced TNF-alpha factor) (Small integral membrane protein of lysosome/late endosome) (p53-induced gene 7 protein)

Protein Class (4)

Disease related genesHuman disease related genesPredicted intracellular proteinsPredicted membrane proteins

Protein Function (3)

Human disease related genes:Nervous system diseases:Neurodegenerative diseases
Disease related genes
Predicted intracellular proteins

Ensembl

ENSG00000189067

Entrez Gene Symbol

LITAF

Gene Synonym (4)

FLJ38636PIG7SIMPLETP53I7

Gene Description

Lipopolysaccharide induced TNF factor

Chromosome

Position

11547722-11636381

Supporting publications (n)

EVMP confidence score

0.50

Fluorescence & Localization4

Tissue Specificlymphoid tissueCell SpecificcDCSingle-Nuclei Brain Specificcentral nervous system macrophage

Function & Pathway6

Protein Function (3)

Human disease related genes:Nervous system diseases:Neurodegenerative diseases
Disease related genes
Predicted intracellular proteins

Cellular Component (11)

Page 1 of 2

Molecular Function (6)

Biological Process (3)

KEGG

hsa04142 Lysosome biogenesis

Mediation Categories (3)

Fusion and delivery mediationImmune mediationReceptor-signaling mediation

Relations & Evidence13

Ligand-Receptor Signaling (10)

10 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
transmembrane	transmembrane	LOCATE	No	No	No	No	No
transmembrane	transmembrane	Ramilowski_location	No	No	No	No	No
transmembrane	transmembrane	OmniPath	No	No	No	No	No
plasma_membrane	plasma_membrane	UniProt_location	No	No	No	No	No
plasma_membrane	plasma_membrane	OmniPath	No	No	No	No	No

Protein Complex Composition (2)

2 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
SIMPLE-HRS-STAM1 complex	HGS LITAF STAM	O14964 Q92783 Q99732	0:0:0	CORUM	CORUM:7287	23166352
	LITAF MDC1 SMAD4 SOD1	P00441 Q13485 Q14676 Q99732	1:1:1:1	NetworkBlastCompleat	Compleat:HC8174

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion Chromatography	Mass spectrometryWestern blotting	2	34265469 36573687

Sequence, Structure & Domains11

Sequences

Length

161

Mass

17,107

Sequence

MSVPGPYQAATGPSSAPSAPPSYEETVAVNSYYPTPPAPMPGPTTGLVTGPDGKGMNPPSYYTQPAPIPNNNPITVQTVYVQHPITFLDRPIQMCCPSCNKMIVSQLSYNAGALTWLSCGSLCLLGCIAGCCFIPFCVDALQDVDHYCPNCRALLGTYKRL

Alternative Products

Event=Alternative splicing; Named isoforms=3; Name=1; IsoId=Q99732-1; Sequence=Displayed; Name=2; IsoId=Q99732-2; Sequence=VSP_016461; Name=3; IsoId=Q99732-3; Sequence=VSP_045701

Alternative Sequence

127..161; CIAGCCFIPFCVDALQDVDHYCPNCRALLGTYKRL -> VHSGLLLHPLLRGCPAGRGPLLSQLQSSPGHLQAFVGLSQTWREPGAAGSPFHLSSSFTPGGGSALVVSPLQGAHLHVFFWGEYVAKLTNLQTPEIAAWSRA (in isoform 2); 127..161; CIAGCCFIPFCVDALQDVDHYCPNCRALLGTYKRL -> QECSGTIVALRSFDLLGSCNPPSSAS (in isoform 3)

Domain & Motif Annotations

Motif

17..20; PSAP motif; important for interaction with TSG101; 20..23; PPxY motif

Domain (CC)

The PPxY motif mediates interaction with WWOX and NEDD4..; DOMAIN: The LITAF domain is stabilized by a bound zinc ion (PubMed:27582497, PubMed:27927196). The LITAF domain contains an amphipathic helix that mediates interaction with lipid membranes (PubMed:23166352, PubMed:27582497, PubMed:27927196). It interacts specifically with phosphatidylethanolamine lipid headgroups, but not with phosphoglycerol, phosphocholine, phosphoserine or inositolhexakisphosphate (PubMed:27927196).

Domain (FT)

76..160; LITAF

Region

1..22; Disordered; 111..134; Membrane-binding amphipathic helix

Protein Families

CDIP1/LITAF family

Sequence Similarities

Belongs to the CDIP1/LITAF family.

Clinical Relevance5

Disease Involvement (4)

Charcot-Marie-Tooth diseaseDisease variantNeurodegenerationNeuropathy

Antibody

HPA006960

Interaction Protein (5)

ENSG00000069869ENSG00000074319ENSG00000116983ENSG00000151929ENSG00000153179

Interaction Count

Interaction Dataset

intact_biogrid

Supporting Publications6

PMID	Title	Abstract
29436780	Autosomal Tubulointerstitial Kidney Disease-MUC1 Type: Differential Proteomics Suggests that Mutated MUC1 (insC) Affects Vesicular Transport in Renal Epithelial Cells.	No abstract available
32284825	Unravelling the proteomic landscape of extracellular vesicles in prostate cancer by density-based fractionation of urine.	No abstract available
33204424	Proteomic analysis of extracellular vesicles reveals an immunogenic cargo in rheumatoid arthritis synovial fluid.	No abstract available
35611462	Extracellular vesicles expressing CEACAM proteins in the urine of bladder cancer patients.	No abstract available
37786918	Rapid and in-depth proteomic profiling of small extracellular vesicles for ultralow samples.	No abstract available
38321535	Identification of specific markers for human pluripotent stem cell-derived small extracellular vesicles.	No abstract available