Protein detail
SHRPN
Sharpin (Shank-associated RH domain-interacting protein) (Shank-interacting protein-like 1) (hSIPL1)
Entry name SHRPN | UniProt ID | EVMP score 0.38 |
Frequency 2 | Transmembrane count | Protein classification Predicted intracellular proteins |
EVMP score: annotation confidence score.
Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40
Basic Information
Protein Names
Sharpin (Shank-associated RH domain-interacting protein) (Shank-interacting protein-like 1) (hSIPL1)
Protein Class
Predicted intracellular proteins
Protein Function
Predicted intracellular proteins
Ensembl
Entrez Gene Symbol
Gene Synonym
DKFZP434N1923SIPL1
Gene Description
SHANK associated RH domain interactor
Chromosome
8
Position
144098633-144108124
Frequency
2
EVMP Score
0.38
Fluorescence & Localization
Tissue SpecificliverCell SpecificHepatocytes
Function & Pathway
Protein Function
Predicted intracellular proteins
Cellular Component
Molecular Function
- GO:0004842 ubiquitin-protein transferase activity
- GO:0005515 protein binding
- GO:0030674 protein-macromolecule adaptor activity
- GO:0031593 polyubiquitin modification-dependent protein binding
- GO:0042802 identical protein binding
- GO:0043130 ubiquitin binding
- GO:0044877 protein-containing complex binding
- GO:0046872 metal ion binding
Biological Process
Reactome
Mediation Categories
Immune mediationReceptor-signaling mediation
Relations & Evidence
Enzyme-Mediated Modification
Ligand-Receptor Signaling
6 records.
| Category | Parent | Database | Transmitter | Receiver | Secreted | Plasma Membrane (Transmembrane) | Plasma Membrane (Peripheral) |
|---|---|---|---|---|---|---|---|
| intracellular | intracellular | ComPPI | No | No | No | No | No |
| intracellular | intracellular | GO_Intercell | No | No | No | No | No |
| intracellular | intracellular | UniProt_location | No | No | No | No | No |
| intracellular | intracellular | OmniPath | No | No | No | No | No |
| adhesion_cytoskeleton_adaptor | intracellular_intercellular_related | Adhesome | Yes | No | No | No | No |
| intracellular_intercellular_related | intracellular_intercellular_related | OmniPath | Yes | No | No | No | No |
Regulatory Interaction Network
1 record.
| Source Protein Symbol | Source UniProt ID | Target Protein Symbol | Target UniProt ID | Is Directed | Is Stimulation | Is Inhibition | Database | References |
|---|---|---|---|---|---|---|---|---|
| MK03 | P27361 | SHRPN | Q9H0F6 | Yes | No | No | PhosphoSitePhosphoSite_ProtMapperProtMapper | PhosphoSite:33392484 |
Protein Complex Composition
6 records.
| Component Name | Component Gene Symbols | Component UniProt ID | Stoichiometry | Database | Database IDs | References |
|---|---|---|---|---|---|---|
| LUBAC complex | RBCK1RNF31SHARPIN | Q96EP0Q9BYM8Q9H0F6 | 1:1:1 | KEGG-MEDICUSComplexPortalCompleatCORUM | PDB:5X0WCORUM:6178PDB:4DBGCompleat:HC1452intact:EBI-2881460 | 1700653733692228147552922145518019136968 |
| LIPFLIPNLRSAM1SHARPIN | P07098Q5VXI9Q6UWE0Q9H0F6 | 0:0:0:0 | hu.MAP2 | |||
| LIPFLRSAM1RNF31SHARPIN | P07098Q6UWE0Q96EP0Q9H0F6 | 0:0:0:0 | hu.MAP | |||
| LIPFLRSAM1SHARPIN | P07098Q6UWE0Q9H0F6 | 0:0:0 | hu.MAP2 | |||
| RNF31SHARPIN | Q96EP0Q9H0F6 | 4:4 | PDB | PDB:5x0w | ||
| SHARPIN | Q9H0F6 | 4 | PDB | PDB:4emoPDB:8k6p |
Isolation & Detection Technology
1 record.
| EV Isolation Method | Detection Method | Number of References | References |
|---|---|---|---|
| Differential Ultracentrifugation | Western blotting | 1 | 39996590 |
Sequence, Structure & Domains
Sequences
Length
387
Mass
39,949
Sequence
MAPPAGGAAAAASDLGSAAVLLAVHAAVRPLGAGPDAEAQLRRLQLSADPERPGRFRLELLGAGPGAVNLEWPLESVSYTIRGPTQHELQPPPGGPGTLSLHFLNPQEAQRWAVLVRGATVEGQNGSKSNSPPALGPEACPVSLPSPPEASTLKGPPPEADLPRSPGNLTEREELAGSLARAIAGGDEKGAAQVAAVLAQHRVALSVQLQEACFPPGPIRLQVTLEDAASAASAASSAHVALQVHPHCTVAALQEQVFSELGFPPAVQRWVIGRCLCVPERSLASYGVRQDGDPAFLYLLSAPREAPATGPSPQHPQKMDGELGRLFPPSLGLPPGPQPAASSLPSPLQPSWSCPSCTFINAPDRPGCEMCSTQRPCTWDPLAAAST
Alternative Products
Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q9H0F6-1; Sequence=Displayed; Name=2; Synonyms=hSIPL1A; IsoId=Q9H0F6-2; Sequence=VSP_023837, VSP_023838
Alternative Sequence
309..326; TGPSPQHPQKMDGELGRL -> QLVLSFLHLHQCPRPPWL (in isoform 2); 327..387; Missing (in isoform 2)
3D Structural Models
Turn
3..5
Helix
12..15; 31..33; 74..76; 106..119; 250..261; 265..267; 283..285
Beta Strand
7..11; 20..30; 40..48; 56..60; 70..73; 77..83; 86..90; 99..105; 219..226; 239..244; 268..271; 293..299
3D Structure
X-ray crystallography (3)
Domain & Motif Annotations
Compositional Bias
122..132; Polar residues; 339..349; Low complexity
Zinc Finger
348..377; RanBP2-type
Domain (CC)
The Ubiquitin-like domain is required for the interaction with RNF31..; DOMAIN: The RanBP2-type zinc fingers mediate the specific interaction with ubiquitin. Binds preferentially linear polyubiquitin chains and 'Lys-63'-linked polyubiquitin chains over 'Lys-48'-linked polyubiquitin chains. Also binds monoubiquitin.
Domain (FT)
219..288; Ubiquitin-like
Region
1..180; Self-association; 122..169; Disordered; 175..310; Interaction with SHANK1; 305..349; Disordered