Protein detail

S12A5

Solute carrier family 12 member 5 (Electroneutral potassium-chloride cotransporter 2) (K-Cl cotransporter 2) (hKCC2) (Neuronal K-Cl cotransporter)

Protein symbol S12A5	UniProt ID Q9H2X9	EVMP score 0.25
Frequency 1	Transmembrane count 12	Protein classification Disease related genesFDA approved drug targetsHuman disease related genesMetabolic proteinsPredicted intracellular proteinsPredicted membrane proteinsTransporters

Basic Information

Protein Names

Solute carrier family 12 member 5 (Electroneutral potassium-chloride cotransporter 2) (K-Cl cotransporter 2) (hKCC2) (Neuronal K-Cl cotransporter)

Protein Class

Disease related genesFDA approved drug targetsHuman disease related genesMetabolic proteinsPredicted intracellular proteinsPredicted membrane proteinsTransporters

Protein Function

Predicted intracellular proteins
Human disease related genes:Nervous system diseases:Epilepsy
Transporters:Electrochemical Potential-driven transporters
Disease related genes
FDA approved drug targets:Small molecule drugs

Transmembrane

99..120; Discontinuously helical; Name=1; 130..151; Helical; Name=2; 175..203; Helical; Name=3; 230..250; Helical; Name=4; 251..276; Helical; Name=5; 403..420; Helical; Name=6; 430..453; Helical; Name=7; 486..513; Helical; Name=8; 535..555; Helical; Name=9; 556..578; Helical; Name=10; 593..615; Helical; Name=11; 616..632; Helical; Name=12

Transmembrane Count

Ensembl

ENSG00000124140

Entrez Gene Symbol

SLC12A5

Gene Synonym

KCC2KIAA1176

Gene Description

Solute carrier family 12 member 5

Chromosome

Position

46021690-46060150

Frequency

EVMP Score

0.25

Fluorescence & Localization

Cell SpecificAstrocytesSingle-Nuclei Brain Specificendothelial cellBlood Cell Specificneutrophil

Function & Pathway

Protein Function

Predicted intracellular proteins
Human disease related genes:Nervous system diseases:Epilepsy
Transporters:Electrochemical Potential-driven transporters
Disease related genes
FDA approved drug targets:Small molecule drugs

Cellular Component

Molecular Function

Biological Process

KEGG

hsa04727 GABAergic synapse

Reactome

Mediation Categories

Clinical-translation mediationFusion and delivery mediation

Relations & Evidence

Enzyme-Mediated Modification

13 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
SLC12A5	LEP	P41159	T	1,030	phosphorylation	REACH_ProtMapperProtMapper	ProtMapper:30106375
SLC12A5	LEP	P41159	T	929	phosphorylation	REACH_ProtMapperProtMapper	ProtMapper:30106375
SLC12A5	OXT	P01178	S	963	phosphorylation	REACH_ProtMapperSparser_ProtMapperProtMapper	ProtMapper:27052180

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Ligand-Receptor Signaling

23 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
cell_surface	cell_surface	OmniPath	No	No	No	No	No
receptor	receptor	scConnect	No	Yes	No	No	No
transmembrane	transmembrane_predicted	Phobius	No	No	No	No	No

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Regulatory Interaction Network

1 record.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
WNK3	Q9BYP7	S12A5	Q9H2X9	Yes	No	Yes	REACH_ProtMapperSIGNORProtMapper	ProtMapper:24139641 SIGNOR:21613606

Protein Complex Composition

2 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
	DCK DGUOK ERC2 SLC12A5	O15083 P27707 Q16854 Q9H2X9	0:0:0:0	hu.MAP
	SLC12A5	Q9H2X9	2	PDB	PDB:7d8zPDB:6m23

Isolation & Detection Technology

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion Chromatography	Mass spectrometry	2	27487081 35763629

Sequence, Structure & Domains

Sequences

Length

1,139

Mass

126,184

Sequence

MSRRFTVTSLPPAGPARSPDPESRRHSVADPRHLPGEDVKGDGNPKESSPFINSTDTEKGKEYDGKNMALFEEEMDTSPMVSSLLSGLANYTNLPQGSREHEEAENNEGGKKKPVQAPRMGTFMGVYLPCLQNIFGVILFLRLTWVVGIAGIMESFCMVFICCSCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLAYLFPAMAIFKAEDASGEAAAMLNNMRVYGTCVLTCMATVVFVGVKYVNKFALVFLGCVILSILAIYAGVIKSAFDPPNFPICLLGNRTLSRHGFDVCAKLAWEGNETVTTRLWGLFCSSRFLNATCDEYFTRNNVTEIQGIPGAASGLIKENLWSSYLTKGVIVERSGMTSVGLADGTPIDMDHPYVFSDMTSYFTLLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPTGTILAIATTSAVYISSVVLFGACIEGVVLRDKFGEAVNGNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAISRDGIVPFLQVFGHGKANGEPTWALLLTACICEIGILIASLDEVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFRYYHWTLSFLGMSLCLALMFICSWYYALVAMLIAGLIYKYIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLVRVDQDQNVVHPQLLSLTSQLKAGKGLTIVGSVLEGTFLENHPQAQRAEESIRRLMEAEKVKGFCQVVISSNLRDGVSHLIQSGGLGGLQHNTVLVGWPRNWRQKEDHQTWRNFIELVRETTAGHLALLVTKNVSMFPGNPERFSEGSIDVWWIVHDGGMLMLLPFLLRHHKVWRKCKMRIFTVAQMDDNSIQMKKDLTTFLYHLRITAEVEVVEMHESDISAYTYEKTLVMEQRSQILKQMHLTKNEREREIQSITDESRGSIRRKNPANTRLRLNVPEETAGDSEEKPEEEVQLIHDQSAPSCPSSSPSPGEEPEGEGETDPEKVHLTWTKDKSVAEKNKGPSPVSSEGIKDFFSMKPEWENLNQSNVRRMHTAVRLNEVIVKKSRDAKLVLLNMPGPPRNRNGDENYMEFLEVLTEHLDRVMLVRGGGREVITIYS

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; Synonyms=KCC2a; IsoId=Q9H2X9-1; Sequence=Displayed; Name=2; Synonyms=KCC2b; IsoId=Q9H2X9-2; Sequence=VSP_029909

Alternative Sequence

1..40; MSRRFTVTSLPPAGPARSPDPESRRHSVADPRHLPGEDVK -> MLNNLTDCEDGDGGANP (in isoform 2)

3D Structural Models

Turn

152..154; 156..162; 192..194; 300..302; 371..373; 477..479; 489..495; 521..524; 572..574; 577..579; 618..620; 757..760; 808..810; 904..906; 923..927; 946..948; 953..957; 1107..1110; 1118..1120

Helix

82..88; 99..102; 103..105; 122..125; 127..133; 137..141; 143..149; 163..177; 188..191; 198..201; 203..228; 245..270; 272..277; 279..299; 341..344; 356..359; 378..380; 423..430; 431..433; 437..441; 450..463; 466..476; 482..485; 500..502; 508..520; 525..538; 543..549; 559..567; 569..571; 581..605; 621..634; 638..644; 646..663; 667..681; 713..722; 738..741; 743..756; 773..782; 812..823; 834..836; 859..868; 894..903; 931..940; 1078..1087; 1104..1106; 1111..1117

Beta Strand

241..243; 308..314; 326..328; 332..334; 348..352; 364..369; 395..397; 412..414; 442..446; 503..505; 555..558; 614..616; 664..666; 685..687; 688..691; 698..700; 729..736; 763..772; 793..796; 803..806; 827..832; 850..852; 854..856; 871..876; 880..885; 887..889; 910..915; 949..951; 1088..1091; 1093..1096; 1122..1128

3D Structure

Electron microscopy (2)

Domain & Motif Annotations

Compositional Bias

19..45; Basic and acidic residues; 46..55; Polar residues; 98..111; Basic and acidic residues; 945..962; Basic and acidic residues; 982..994; Acidic residues; 1003..1012; Low complexity; 1023..1042; Basic and acidic residues

Region

1..63; Disordered; 92..116; Disordered; 667..681; Scissor helix; 942..1052; Disordered

Protein Families

SLC12A transporter family
K/Cl co-transporter subfamily

Sequence Similarities

Belongs to the SLC12A transporter family. K/Cl co-transporter subfamily.

Clinical Relevance

Disease Involvement

Disease variantEpilepsyFDA approved drug targets

Related Diseases

Epilepsy/seizure

Biomarker

Investigative

Drug Targets

FDA approved drug targets

Drugs

BUMETANIDE

Antibody

HPA072058