Protein detail

PKHA4

Pleckstrin homology domain-containing family A member 4 (PH domain-containing family A member 4) (Phosphoinositol 3-phosphate-binding protein 1) (PEPP-1)

Entry name PKHA4	UniProt ID Q9H4M7	EVMP confidence score 0.50
Supporting publications (n) 4	Transmembrane count	Protein classification Predicted intracellular proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

Pleckstrin homology domain-containing family A member 4 (PH domain-containing family A member 4) (Phosphoinositol 3-phosphate-binding protein 1) (PEPP-1)

Protein Class

Predicted intracellular proteins

Protein Function

Predicted intracellular proteins

Ensembl

ENSG00000105559

Entrez Gene Symbol

PLEKHA4

Gene Synonym

PEPP1

Gene Description

Pleckstrin homology domain containing A4

Chromosome

Position

48837092-48868617

Supporting publications (n)

EVMP confidence score

0.50

Fluorescence & Localization2

Tissue SpecificbrainSingle-Nuclei Brain SpecificBergmann glia

Function & Pathway5

Protein Function

Predicted intracellular proteins

Cellular Component (3)

Molecular Function (6)

Reactome (4)

Mediation Categories (2)

Metabolism mediationReceptor-signaling mediation

Relations & Evidence6

Ligand-Receptor Signaling (4)

4 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No

Protein Complex Composition (1)

1 record.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
	KLHL12 PLEKHA4	Q53G59 Q9H4M7	4:4	PDB	PDB:8oio

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationUltrafiltration / Tangential Flow FiltrationSize Exclusion ChromatographyImmunoaffinity Capture	Mass spectrometryWestern blotting	32	34889606 38938674 39290459 23376485 26538482 31508500 3467568 39443544 37702715 32795414 29562735 28562335 24700864 2353145 25471207 26801919 28242843 30071318 30608700 32927759 33580265 33729255 22106071 22418980 36635400 37686366 40098346 41201090 34131392 30760538 40311616 41315767

Sequence, Structure & Domains11

Sequences

Length

779

Mass

85,401

Sequence

MEGSRPRSSLSLASSASTISSLSSLSPKKPTRAVNKIHAFGKRGNALRRDPNLPVHIRGWLHKQDSSGLRLWKRRWFVLSGHCLFYYKDSREESVLGSVLLPSYNIRPDGPGAPRGRRFTFTAEHPGMRTYVLAADTLEDLRGWLRALGRASRAEGDDYGQPRSPARPQPGEGPGGPGGPPEVSRGEEGRISESPEVTRLSRGRGRPRLLTPSPTTDLHSGLQMRRARSPDLFTPLSRPPSPLSLPRPRSAPARRPPAPSGDTAPPARPHTPLSRIDVRPPLDWGPQRQTLSRPPTPRRGPPSEAGGGKPPRSPQHWSQEPRTQAHSGSPTYLQLPPRPPGTRASMVLLPGPPLESTFHQSLETDTLLTKLCGQDRLLRRLQEEIDQKQEEKEQLEAALELTRQQLGQATREAGAPGRAWGRQRLLQDRLVSVRATLCHLTQERERVWDTYSGLEQELGTLRETLEYLLHLGSPQDRVSAQQQLWMVEDTLAGLGGPQKPPPHTEPDSPSPVLQGEESSERESLPESLELSSPRSPETDWGRPPGGDKDLASPHLGLGSPRVSRASSPEGRHLPSPQLGTKAPVARPRMSAQEQLERMRRNQECGRPFPRPTSPRLLTLGRTLSPARRQPDVEQRPVVGHSGAQKWLRSSGSWSSPRNTTPYLPTSEGHRERVLSLSQALATEASQWHRMMTGGNLDSQGDPLPGVPLPPSDPTRQETPPPRSPPVANSGSTGFSRRGSGRGGGPTPWGPAWDAGIAPPVLPQDEGAWPLRVTLLQSSF

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q9H4M7-1; Sequence=Displayed; Name=2; IsoId=Q9H4M7-2; Sequence=VSP_009770, VSP_009771, VSP_009772

Alternative Sequence

325..349; Missing (in isoform 2); 582..608; APVARPRMSAQEQLERMRRNQECGRPF -> SKEHHPLLADFRRSPGAGSQPLPSPGY (in isoform 2); 609..779; Missing (in isoform 2)

3D Structural Models

Helix

101..103; 138..152

Beta Strand

55..64; 66..69; 72..80; 83..89; 97..100; 105..108; 116..124; 131..134

3D Structure

X-ray crystallography (3)

Domain & Motif Annotations

Compositional Bias

184..193; Basic and acidic residues; 315..332; Polar residues; 525..535; Low complexity; 536..551; Basic and acidic residues; 594..603; Basic and acidic residues; 647..663; Polar residues; 704..724; Pro residues

Domain (FT)

54..153; PH

Region

152..352; Disordered; 492..670; Disordered; 691..764; Disordered

Clinical Relevance1

Antibody (2)

HPA042978 HPA048473

Supporting Publications4

PMID	Title	Abstract
28986585	Quantitation of putative colorectal cancer biomarker candidates in serum extracellular vesicles by targeted proteomics.	No abstract available
33204424	Proteomic analysis of extracellular vesicles reveals an immunogenic cargo in rheumatoid arthritis synovial fluid.	No abstract available
34817906	Proteomic dissection of large extracellular vesicle surfaceome unravels interactive surface platform.	No abstract available
37786918	Rapid and in-depth proteomic profiling of small extracellular vesicles for ultralow samples.	No abstract available